Objective To investigate the value of fetal heart monitoring combined with umbilical blood flow for the prediction of perinatal outcome.Methods 2200 women with pregnancy for 26 to 42 weeks were given the detection of S/D value of fetal umbilical artery blood flow and Non Stress Test(NST) of fetal heart monitoring,and the relationship between the NST,S/D and prediction of perinatal outcome.Results When the S/D value ＜3,perinatal anmiotic fluid shortage,amniotic fluid pollution,newborn children hypoxia asphyxia,newborn child deformity and the probability of death were significantly less than S/D ≥ 3(x2 =15.61,6.2.4,12.38,9.73,5.77,all P ＜ 0.05);when the NST ＞7,the probability of adverse perinatal outcome was significantly less than the NST≤7(x2 =4.54,4.12,5.89,5.94,8.10,all P ＜ 0.05).Conclusion The fetal umbilical artery blood flow monitoring and fetal heart monitor could improve the accuracy of prediction of perinatal outcome,avoid the defects of a single method of monitoring,early and timely diagnose the fetal distress,and give reasonable treatment to reduce neonatal mortality.

Objective To explore the cause of acute urinary retention (AUR) ,the functional change of bladder detrusor. Methods The clinical data in 178 patients with AUR combined with urodynamic study were retrospectively analyzed. Results In male patients,the main cause of AUR was bladder oudet obstruction(BOO) ,the percent was 60.63%(77/127) ,in age more than 50 years old patients, while, in age less than 50 years old patients,the main cause was detrusor arcflexia (DUA), the percent was 72.55% (37/51), because of trauma of Iumbosacral vertebrae. In the female patients, the main cause of AUR was DUA. Between male and female patients with age more than 50 years old, there was significant difference in the cases of low compliance and DUA (P < 0.01) ,unstable bladder and BOO (P < 0.05). Conclusions The main cause of AUR in elderly male patients is BOO, which induces low eompliance and unstable bladder. While the main cause of AUR in female patients and young-middle age male is DUA .The function of detrusor changes differently with various causes.

Objective To compare the efficacies of several commonly-used penetrating needling methods in treating post-stroke strephenopodia. Method Eighty-three patients with post-stroke strephenopodia were randomized into four groups, a control group intervened by conventional needling method (n=20), and the rest three groups were additionally intervened by different penetrating needling methods: penetrating group 1 [Qiuxu (GB40) towards Zhaohai (KI6), n=21], penetrating group 2 [Taibai (SP3) towards Shugu (BL65), n=21], and penetrating group 3 [Jiaoxin (KI8) towards Fuyang (BL59), n=21], to compare their effects on the function of ankle and lower extremity. Result The penetrating needling methods and conventional needling method all can significantly improve the function of ankle in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the rest groups (P<0.01), the effectiveness of penetrating group 2 was more significant than that of penetrating group 3 and the control group (P<0.05,P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 3 and the control group (P > 0.05). The penetrating needling methods and conventional needling method all can significantly improve the motor function of lower extremity in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the other groups (P<0.01), the efficacies of penetrating group 2 and 3 were more significant than that of the control (P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 2 and 3 (P>0.05). Conclusion Needling from Qiuxu towards Zhaohai and from Taibai towards Shugu can produce significant effects in improving ankle function and the motor function of lower extremity in patients with post-stroke strephenopodia, while needling from Jiaoxin to Fuyang doesn’t have notable advantage compared to the other penetrating needling methods and conventional needling method.

Objective To construct eukaryotic expression plasmid pEGFP-N1/EPO and to detect its expression in vitro in order to provide experimental evidence for further study on the treatment of hypoxic-ischemic brain damage.Methods The total RNA was extracted from rat kidney.EPO cDNA fragment was obtained by RT-PCR amplication,inserted into pEGFP-N1 vector and identified by restriction endonuclease digestion and nucleotide sequencing.MSCs cells were transfected with the plasmid using Lipofectamine 2000.The expression of EPO protein was detected by immunohistochemistry technique and RT-PCR.Results Confirmed by restriction endonuclease digestion and sequencing,the recombinant plasmid contained the sequence of rat EPO gene was successfully constructed.After transfection with the plasmid,EPO protein could be expressed in MSCs cells.Conclusion The constructed eukaryotic expression vector pEGFP-N1/EPO can express rat EPO in vitro.

OBJECTIVE:To analyze the fatty acids in rinds or seeds of Pittosporum tobira.METHODS:The oil from rinds or seeds of Pittosporum tobira was extracted with organic solvent,and the fatty acids were methyl-esterified with KOH-NaOH solution and identified by GC-MS.RESULTS:13 fatty acids were identified from rinds and seeds of Pittosporum tobira,mostly the palmitic acid and oleic acid.CONCLUSION:Both rinds and seeds of Pittosporum tobira can be exploited as an edible oil for health care.

OBJECTIVE:To analyze the components of the volatile oil in different parts of Lonicera japonica Thunb.from Henan province.METHODS:The volatile oil was extracted from the buds,leaves,stems of L.japonica Thunb.by solid-phase micro-extraction,and the chemical compositions were identified by GC-MS combined with Kvotas retention index.The relative percentage of each constituent was determined by GC area normalization method.RESULTS:Thirty-nine compounds were identified from different parts of L.japonica Thunb.from Henan province,and seven of which were mutual in the buds,leaves,stems of L.japonica Thunb..CONCLUSION:This study serves as a scientific basis for the further development and utilization of L.japonica Thunb..

OBJECTIVE: To analyze the volatile components in the Eriobotrya japonica by headspace solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS) and to optimize the operating conditions for SPME.METHODS: DB-5 MS elastic silica capillary column(30.0 m?250 ?m?0.25 ?m) was used as the chromatographic column with helium of high purity(99.999%) as carrier gas at a flow rate of 1.0 mL?min-1.The injector temperature was 250 ℃;MS condition: ionization mode was EI with electron energy of 70 eV and the mass scan range of 30～440 amu.RESULTS: After headspace absorption for 30 min at 80 ℃ using 65 ?m polydimethylsiloxane-divinyl-benzene extraction head followed by desorption for 1 min at 250 ℃,the E.japonica sample was separated and identified by GC-MS analysis.A total of 34 peaks were obtained and 29 components were identified which accounted for 97.42% of the total peak area,topping the list were benzaldehyde and 4-methoxybenzaldehyde.CONCLUSION: HS-SPME-GC-MS can be used for the rapid analysis of the volatile components in E.japonica.

To evaluate the significance of plasma ACTH, serum cortisol and UFC levels as well as ACTH stimulation test, aminoglutethimide test and insulin induced hypoglycemia test in patients with adrenal insufficiency. Sixteen patients with primary insufficiency (Addison`s disease) and 75 cases with secondary insufficiency (hypopituitarism) diagnosed from 1985 to 1999 were reviewed. It was found that high levels of ACTH were detected in all patients with primary insufficiency, and in most of them the levels of cortisol and UFC were low. No response of cortisol and UFC were observed after ACTH stimulation in the patients with primary insufficiency, but normal or delayed action was observed in the patients with secondary adrenal insufficiency. In most of secondary adrenal insufficiency the ACTH levels were in normal range but the cortisol and UFC levels were low; the elevated action of ACTH by insulin induced hypoglycemia test or aminoglutethimide test did not appear in the patients with secondary adrenal insufficiency. The results suggested that the low levels of cortisol and UFC were a common feature in the most of patients both with primary or secondary adrenal insufficiency. The ACTH level was the key indicator to distinguish the primary adrenal insufficiency from secondary adrenal insufficiency, as the ACTH levels were remarkably elevated in primary patients but were normal or decreased in secondary insufficiency patients. The ACTH stimulation test was useful in the diagnosis of the primary or secondary adrenal insufficiency. The insulin induced hypoglycemia test or aminoglutethimide test was valuable in the diagnosis of the secondary adrenal insufficiency.

To evaluate the significance of plasma ACTH (pACTH), serum cortisol (SC) and urinary free cortisol UFC as well as low or high dose dexamethasone suppression test in Cushing syndrome, the levels of hormones were evaluated in 136 cases of Cushing syndrome. It was found that the levels of SC and UFC were elevated in almost all the 121 patients with Cushing syndrome. The mean levels of pACTH were higher in 86 patients with Cushing disease and lower in 36 cases with adrenocortical tumor or ACTH independent nodular adrenocortical hyperplasia (AINAH) than those in normal subjects. The ACTH levels might be in normal range in many patients with Cushing syndrome, but they were higher in patients with Cushing disease and lower in the patients with adrenocortical tumor or AINAH than those of the high limit at ACTH0am in normal subjects. It was a common feature that the diurnal rhythm of hormone secretion disappeared and the hormone levels were not suppressed by low dose dexamethasone suppression test in the patients with Cushing syndrome. In more than 90% of the patients with adrenocortical tumor or AINAH the levels of SC and UFC were not suppressed by high dose dexamethasone. In 12 of clinically cured patients with Cushing disease the levels of pACTH8am, SC8am and UFC were normal or even low. The pACTH levels were low in two patients with iatrogenic Cushing syndrome. The results suggested that examinations of SC, UFC and low dose dexamethasone suppression test were very important in the diagnosis of Cushing syndrome. The pACTH level was a key parameter to distinguish Cushing disease from adrenocortical tumor or AINAH.

Objective To investigate the efficacy and safety of slow release paclitaxel eluting stents. Methods The 148 lesions of 71 patients were treated and 171 stents were implanted, of which 132 were slow release paclitaxel eluting stents and were implanted in 102 lesions. Results All except 1 of the slow release paclitaxel eluting stents were successfully implanted. No complications occurred during hospitalization. There were no cardic events and ischemic ECG evidence in 48 patients of the 6 months′ follow-up. Conclusion The efficacy and safety of slow release paclitaxel eluting stents within 6 months have been approved.