Objective To investigate blood pressure and blood lipids change in official staff population. Methods Official staffs were divided into normal blood pressure group, pre-hypertension group, hypertension group and hypertension under control group based on their blood pressure, and hypertension incidence in pre-hypertension group,hypertension group were studied, hypertension incidence in pre-hypertension group and normal blood pressure group were compared; all staff were divided into normal blood lipids group and high blood lipids group based on their blood lipids. Results Hypertension incidence of official staffs was increasing year by year; hypertension incidence of pre-hypertension group was apparently higher than that of normal blood pressure group (P < 0.01) ; the incidence of hypertension in high blood lipids group(total cholesterol and triglyceride) was apparently higher than that of normal blood lipids group(P < 0.05). Conclusion Life style intervention should be performed on official staffs, especially for pre-hypertension group, blood pressure and blood lipids tracing should be applied on them,in order to decrease the incidence of hypertension and cardiac and cerebral vascular stroke.

Objective To find a simple and effective way for pericardial effusion.Methods Arterial sheath were applied to patients with pericardial effusion when pericardium puncture were performed,and negative pressure drainage bag were connected and aspirated interruptively to no aspiration.1～2 days later,arterial sheath were drawn out.Results 40 cases of pericardial effusion were effectively cured by this method,and clinical effective rate was 100%,and no complication associated with arterial sheath placement was observed.Conclusion Arterial sheath placement is an effective,safe and convenient method for the treatment for pericardial effusion.This method can be routinely applied to pericardial effusion due to different causes.

Objective To observe the effects of simvastatin o n the prognosis of patients with chronic heart failure with coronary heart diseas e.Methods During 2001-12~2003-12,114 cases were divided randomly into two groups:the treatment group(n=59),who received simvastatin 20mg QN,a nd the control group(n=55) .All patients with heart failure were treated by conventional therapy.Results Compared with the control group,the total cholesterol ( TC),LDL-C,C-RP of the patients in the treatment group were all degraded to dif ferent degrees and the LVEF was elevated obviously.The frequency of hospitaliza tion and the time were lower than that in the control group but were not signifi cantly different;the death rate was significantly lower than that in the control group(P

Oncolytic viruses have made great breakthroughs in cancer treatment, especially for reovirus, which can effectively induce the death of tumor cells without harming the normal tissues. More than 80％ tumor cells are sensitive to reovirus infection. Reovirus induces the apoptosis of tumor cells and exerts anti-tumor immunity to achieve anti-tumor activity, and the curative effect of combination therapy with reovirus and chemotherapeutic drugs exceeds the effect of monotherapy. Reovirus can exert anti-tumor effects through different mechanisms, which is of great significance for the new and effective treatment of tumors in the future.

Objective:To identify the risk factors for postoperative pulmonary complications (PPCs) in pediatric patients undergoing malignant tumor resection.Methods:Medical records of pediatric patients who underwent open abdominal malignant tumor resection at Children′s Hospital affiliated to Zhejiang University School of Medicine from August 2019 to July 2023, with length of hospital stay ≥48 h, were retrospectively collected. Patients were divided into PPC group and non-PPC group based on the occurrence of PPCs within 7 days postoperatively. Variables with P<0.05 in the univariate analysis were included in the binary logistic regression analysis to identify the risk factors for PPCs. Results:A total of 605 pediatric patients who underwent abdominal malignant tumor resection were finally included, among which 391 children developed PPCs, with an incidence of 64.6%. Binary logistic regression analysis showed that body weight ( P=0.001), American Society of Anesthesiologists Physical Status classification ( P<0.001), preoperative hypoalbuminemia ( P=0.013), preoperative chemotherapy ( P=0.003), tumor compression/encasement of major abdominal vessels ( P=0.002), anesthesia duration ( P<0.001), intraoperative fluid intake (ml·kg -1·h -1, P<0.001), intraoperative use of hypotensive agents ( P=0.047), and concurrent resection of mediastinal tumors via abdominal approach ( P<0.001) were risk factors for PPCs in children undergoing malignant tumor resection. Age ( P<0.001) was identified as a protective factor for PPCs. Conclusions:Body weight, American Society of Anesthesiologists Physical Status classification, preoperative chemotherapy, preoperative hypoalbuminemia, tumor compression/encasement of major abdominal vessels, anesthesia duration, intraoperative fluid intake, intraoperative use of hypotensive agents, and concurrent resection of mediastinal tumors via abdominal approach are risk factors for PPCs in pediatric patients undergoing open abdominal malignant tumor resection, whereas age is a protective factor for PPCs.

Objective: To assess the predictive value of the platelet-to-lymphocyte ratio(PLR), neutrophil-to-lymphocyte ratio(NLR)and tumor-related factors on the peritoneal metastasis in advanced gastric cancer patients. Methods: A total of 701 pathologically confirmed gastric cancer patients who underwent surgery at the First Affiliated Hospital of Wenzhou Medical University between Jan 2009 and Jan 2012 were enrolled. Results: Univariate analysis indicated that tumor location, tumor size, serosal invasion, depth of invasion, pathological type, lymph node metastasis and PLR were related to peritoneal metastasis(all P<0.05). According to the area under the ROC curve, the optimal cutoff value of PLR for predicting peritoneal metastasis was 132.43 (sensitivity 71.8%, specificity 50.6%). There were significant differences in tumor size, TNM stage, lymph node metastasis and serosal infiltration between high PLR group and low PLR group. Multivariate Logistic analyses revealed that PLR (HR=2.205, P=0.003), lymph node metastasis(HR=3.113, P=0.010)and tumor size(HR=1.150, P=0.014) were independent risk factors for peritoneal metesfasis. Conclusions Preoperative PLR is valuable in predicting peritoneal metastasis of advanced gastric cancer and it is an independent risk factor for peritoneal metastasis of gastric cancer.

Objective:To analyze the gene variation and evolutionary characteristics of human parainfluenza virus 3 (HPIV3) circulating in China based on hemagglutinin-neuraminidase (HN) gene.Methods:Multiple qPCR was used to screen nucleic acid of common pathogens in throat swabs from acute respiratory tract infection cases in five provinces (Beijing, Zhejiang, Anhui, Henan, Hunan) from 2007 to 2020. Subsequently, the HN gene sequence of HPIV3 positive samples was determined and compared with the HN gene sequence of HPIV3 strains from 10 provinces in China and abroad in the GenBank database. The molecular evolution analysis was carried out using a variety of bioinformatics method.Results:A total of 49 strains of HPIV3 HN gene sequences were obtained from 5 provinces, with nucleotide homology ranging from 96.6% to 100%. Among them, 48 strains were subtype C3 and 1 strain was subtype C5. Phylogenetic analysis showed that there were co-epidemics of C1, C3 and C5 strains in 12 provinces of China during 2003—2020, and the nucleotide and amino acid homology among the strains were 95.4%-99.8% and 97.9%-100%, respectively. Among them, C3 is the dominant subtype in China, which is divided into five evolutionary branches of C3a, C3b, C3c, C3e and C3f, and the C3f has the widest range and time of spread. The evolutionary analysis of the C3 subtype showed that the estimated time to the most recent common ancestor (tMRCA) of it dated back to 1990, and its effective population size tended to be stable after expansion from 2002 to 2010. The HN gene evolution rate of the evolutionary branches of C3 HPIV3 varied from 3.69×10 -4 to 5.82×10 -4 substitutions/sites/year; the HN protein of C3 subtype strains shared four potential N-glycosylation sites N308, N351, N485 and N523, and the selection pressure was mainly negative. Conclusions:The C3 is an endemic dominant genotype, which has been widely spread and continuously circulating in China, and has formed different evolutionary clades during epidemic.