Objective To investigate the levels of serum neuron-specific enolase (NSE) and C-reactive protein ( CRP ) in patients with acute cerebral infarction, and its value on prognosis. Methods The levels of serum NSE and CRP were compared in 92 acute cerebral infarction patients (observation group) and 45 healthy (control group). The relationship between the levels of NSE, CRP and the degree of neurological damage and infarction location were analyzed. Results The levels of serum NSE and CRP were significantly higher in observation group than those in control group (P< 0.01). In observation group, the levels of serum NSE and CRP were higher in heavy patients [ (35.63 ± 7.12) μg/L, (13.82 ± 3.57) mg/L] than those in mild or medium patients [(17.24 ± 5.32) μg/L, (5.61 ± 2.35) mg/L and (26.08 ±5.91) μg/L, (9.07 ±2.14) mg/L,respectively](P<0.01). There was no significant difference in the levels of serum NSE and CRP in different infarction location(P > 0.05 ). Conclusion The level of serum NSE and CRP is an index in reflecting the degree of neurological damage, and they can be used in evaluating patient's condition and prognosis.

Objective To investigate the T cell clonality in patients with systemic lupus erythematosus (SLE) . Methods T cell clonality in peripheral blood lymphocytes from six patients with active SLE was analyzed using reverse transcriptase- polymerase chain reaction (RT- PCR) and GeneScan technique. Results T cell receptor (TCR) Vβ PCR products from two patients were derived from polyclonal T cells, however, TCR Vβ9, Vβ2, VβI, Vβ2 pioducts from the other four patients were derived from oligoclonal T cells respectively.ConclusionT cells from patients with SLE demonstrated a striking oligoclnality and their clonal expansion may be a specific response driven by autoantigens.

Regional arterial intra-chemotherapy (RAIC) was an effective method of combined therapy for pancreatic carcinoma, which could elevate the regional con-centration of anticancer agents for sufficient dosage in pan-creas, reduce the systemic adverse events, increase the toler-ation of chemotherapy.It could also increase the concentra-tion of anticancer agents in regional lymph node of pancreas, thus decrease the lymph node metastasis.The mechanism of RAIC for pancreatic cancer and the impact for lymph node metastasis needed to be further investigated.

Objective To observe the clinical effect of Shuguanling(Chinese medicine for dredging the oviduct) on salpingemphraxis sterility and to study its mechanism.Methods The diagnosed patients were randomized into 3 groups with 52 in each.The treatment group was administered Shuguanling p.o.and clyster.The control group A was given Xuefu Zhuyu Oral Liquid(Oral liquid for removing blood stasis in the abdomen),and Kangfu Xiaoyan Pills(Pills for recovery of gynecological inflammation) suppository into recta.The control group B was prescribed Xuefu Zhuyu Oral Liquid and vaginal culdocentesis with the mixture of dexamethasone,chymotrypsin,gentamicin and normal saline.After three months,the effect was evaluated and the pelvic blood flow,blood viscosity,plasma collagenase,fibrinogen and fibrinolytic activity of the treatment group were tested for comparison.Results The tubal patency rate and pregnancy rate of treatment group were significantly higher than those of control groups(P

To investigate the predisposing role of HLA-DR genes to systemic lupus erythematosus (SLE), We used polymerase chain reaction and sequence specific oligonucleotide (PCR/SSO) probe hybridization to type HLA-DR subregion in the patients with SLE of Han nationality from Jiangsu province and matched control subjects. The results indicated that DR2 gene frequency was significantly more frequent in patients than that in controls. Whereas DR4 significantly decreased in patients compared with controls. It suggests that DR2 or the other unidentified genes tightly linked to it might be the susceptible genes of SLE. Whereas DR4 might have a protective effect on SLE.

Objective In order to evaluate the therapeutic effect, to screen effective drugs for AIDS, and to find out the changes of patient's condition, it is necessary to develop an objective method to quantitate the levels of viral load of the patients before and after treatment.Method Differential PCR(D PCR) was used to co amplify the target HIV 1 gag gene and reference template ? globin gene, so as to determine the level of HIV replication quantitatively. The levels of HIV DNA in the peripheral blood mononuclear cells of 8 patients with AIDS were determined quantitatively.Results The levels of the provirus of the 8 patients ranged from 0 508 2 210 copies/?g DNA, there were differences in the levels of replication and integration. Conclusion It is the authors′ opinion that this method is easy to control with an advantage of reliability, quickness and reproducibility, It is suitable to measure clinical specimens, and provides an objective evidence for the evaluation of responses to therapeutic intervention.

Objective To investigate the accuracy and reliability of the image automatic matching using X-ray volumetric image (ⅩⅥ) system with the accelerator Elekta Synergy and to compare the matches with different methods and area.Methods A simulation plan using the head and neck phantom was designed and sent to ⅩⅥ system for CBCT scanning.During the scanning,the couch for certain distance was moved and the data of ⅩⅥ image automatic Matching in different matching methods and area was collected.Results The result of ⅩⅥ image automatic matching is consistent and correct to direction and distance which the couch had been moved (x:(0.11 ± 0.41) mm,y:(-0.04± 2.6) mm,z:(0.28 ± 0.74 mm)).There are not significant differences between the results that matching in different matching methods and different matching area.Conclusions The automatic image Matching of ⅩⅥ is accuracy and high reliability in recognition of offset error.But there are some significant differences on the automatic image Matching in different matching methods and different matching area.

Objective To explore the feasibility and safety of breast conserving surgery ( BCS) in patients with early breast cancer after local lumpectomy . Methods Clinical data of 26 patients who previously had received local lumpectomy from January 2009 to December 2011 were retrospectively analyzed .All the patients were diagnosed as solitary invasive ductal carcinoma with preoperative staging of T1N0M0.The interval from lumpectomy to BCS was 6-24 days (mean, 10 days) and the maximum diameter of tumors before first operation was 1-2 cm (mean, 1.6 cm).The shortest distance between nipple and operative incision was 2-6 cm (mean, 4.5 cm). Results The operations were successfully completed in all 26 patients, 15 of which received sentinel lymph node biopsy and 11 of which received axillary lymph node dissection .The hospital stay was 3-5 days with stage-Ⅰhealing of incision .All patients received whole breast radiation therapy after BCS .There was no local recurrence or distant metastasis during 2 -5 year ’ s follow-up (median, 32 months).All the patients were satisfied with the shape of breast and the quality of life . Conclusion For those patients with early breast cancer who have received local lumpectomy , meet the requirement of CBS , and have the willing of breast conserving , BCS is an ideal choice after rigid application of surgical indications .

Objective To evaluate clinical effect of rabeprazole combined with teprenone capsules in treatment of gastric ulcer by marking targeting biopsy and leptin.Methods A total of 118 patients with active gastric ulcer confirmed by endoscopy were randomly divided into two groups.Patients in the treatment group (n=60) were given rabeprazole 10 mg,bid,and teprenone capsules 50 mg,tid.Patients in the control group (n=58) were given rabeprazole 10 mg,bid.Both groups were treated continuously for 56 days.Before and after treatment,2 groups were labeled with biopsy,the clinical efficacy and the healing rate of two groups were recorded,the quality of healing and the expression of leptin were compared.The level of leptin was tested after treatment.Results After 10 days,the difference of clinical curative effect was not statistically significant (P>0.05).After 56 days,the difference of clinical curative effect was statistically significant (P<0.05);ulcer healing rate (93.33%)in treatment group was higher than that of control group (72.41%);ulcer healing quality (93.33%) in treatment group was higher than that of control group (58.62%);leptin level of treatment group was lower than that of the control group;gastric ulcer recurrence rate (3.8%) in treatment group was lower than that of the control group (24.0%) (all P<0.05).Conclusion Rabeprazole combined with teprenone in the treatment of gastric ulcer is better than rabeprazole.Marking targeting biopsy and leptin can be used to evaluate the healing quality of gastric ulcer more accurately,which can be an evaluation index of the quality of gastric ulcer healing and used as an indicator of the quality of gastric ulcer healing.

Objective To study the effect of dexmedetomidine complex with remifentanil in elderly patients with laparoscopic colon cancer resection.Methods 80 cases of elderly patients with laparoscopic colon cancer resec-tion were selected and divided into the study group and the control group with 40 patients in each group according to the random number table method.Two groups were induced conventionally,and intraoperative anesthesia maintained by remifentanil and sevoflurane.The study group were pumped dexmedetomidine 0.8μg/kg 10min before induction, then the doses reduced to 0.2 μg·kg -1 ·h -1 for continuous pumping until to the end of surgery,while the control group was pumped the same dose 0.9% sodium chloride injection,then the heart rate(HR)and mean arterial pres-sure(MAP)of the two groups were compared when entered into surgery room(T0 ),administered 5min(T1 ),after intubation(T2 ),surgical incision(T3 )and after extubation(T4 ),and the compose score,recovery time,and agitation score of two groups were compared.Results HR and MAP of the study group at T1 ,T2 ,T3 and T4 were (62.2 ± 1.1)times/min,(67.3 ±3.2)times/min,(69.2 ±2.3)times/min,(70.2 ±2.5)beats/min and(68.3 ±0.5)mmHg,(70.1 ±0.9)mmHg,(71.5 ±0.9)mmHg,(68.2 ±1.9)mmHg significantly lower than which of the control group (t =9.289,10.293,8.928,9.039,9.278,10.789,9.322,8.399,all P <0.05),and HR and MAP of the control group at T1 ,T2 ,T3 and T4 were significantly higher than T0,the differences were statistically significant(t =8.928, 9.892,8.387,8.927,9,783,9,073,9.039,7.938,all P <0.05);Compose score(4.2 ±1.3)points of the study group was significantly higher than (1.2 ±0.2)points of the control group,but the agitation score of the study group was(1.2 ±0.7)points,which was significantly lower than (2.9 ±1.5)points of the control group,the differences were statistically significant(t =9.38,8.379,all P <0.05).Conclusion Dexmedetomidine complex with remifen-tanil has a anesthetic good effect in elderly patients with laparoscopic colon cancer resection,and which can maintain the more stable hemodynamics.