Based on the pathogenesis and symptom of rheumatoid arthritis (RA), professor LIN Guohua's unique opinion and method for RA in clinical treatment are summarized and analyzed. In the opinion of Professor Lin, RA is considered as "Jinbi" and "Gubi" in TCM, which is caused by deficient root with superficial excess. Based on the symptoms of RA, attention should be focused on lung-kidney diagnosis and treatment, and gold and catgut implantation at acupoint can be mutually combined, which is aimed to provide a special and effective method for clinical treatment of RA.
Acupuncture Points ; Arthritis, Rheumatoid ; therapy ; Female ; Gold ; therapeutic use ; Humans ; Middle Aged ; Prostheses and Implants

Objective To evaluate the value of magnetic resonance spectroscopy (MRS) in the diagnosis of prostate cancer and benign prostate hyperplasia. Methods Fifty-five patients including 36 benign prostate hyperplasia patients and 19 prostate cancer patients,were performed with the scanning of magnetic resonance imaging (MRI) and MRS. MRI observation was focused on the size of the gland,the signal features, the location of the lesion and the extent of the tumor. MRS observation included the chemical shift of citrate(Cit), choline(Cho) and creatine(Cre) spectra, and the ratio of (Cho + Cre) /Cit SPSS 13.0 statistical analysis software was performed for statistic analysis of MRI,MRI + MRS in the diagnosis of prostate cancer to get the sensitivity,specificity and accuracy, respectively. Results Based on the analysis of MRI of prostate cancer and pathological findings, the diagnostic sensitivity,specificity and accuracy of MRI were 78.9%( 15/19),66.7%(24/36),70.9%(39/55), respectively. The diagnostic sensitivity,specificity and accuracy of MRI + MRS were 84.2%(16/19),88.9%(32/36),87.3%(48/55),respectively. There was significant difference between the two methods (P<0.05). Conclusions MRS has made it possible to obtain functional and metabolic information about prostate cancer non-invasively. Combining MRI and MRS are useful for differentiating diagnosis of benign prostate hyperplasia from prostate cancer.

Objective To analyze imaging feature of multislice spiral CT(MSCT)in diagnosis of carotid artery diseases and to evaluate it’s dianostic significance.Methods MSCTA in 80 cases were performed in our study,of them,15 patients were undergone DSA simultaneous.The imaging quality of MSCTA was evaluated and the accuracy of MSCTA in diagnosis of carotid artery diagnosis by comparing the results of CTA and DSA in the same one patient.The calcifications in vessel wall displayed by various CTA reconstruction technique were analysed.Results The results of DSA and MSCTA 60 branches of carotid artery were analysed comparatively.The specificity and sensitivity of MSCTA in diagnosis of carotid artery stenosis was 85.71% and 92.7% respectively,the negative value was 95%,Kappa value was 0.81.Furthermore,CTA could display the artery wall well,it was easy to acquire good imaging quality.Conclusion As one noinvasive method of examination,CTA will play a common role in diagnosis of carotid artery disease.

Objective To compare the efficacy of aspirin and rivaroxaban on prevention of deep vein thrombosis after total knee repalcement.Methods Patients (200 cases) who were given total knee replacement were selected,and the patients in aspirin group (98 cases) were administered with asprin after 12 h treatment,and the patients in rivaroxaban group (102 cases) were administered with rivaroxaban after 12 h treatment.The efficacy of aspirin and rivaroxaban to prevent deep vein thrombosis after total knee repalcement was evaluated by blood coagulation indexes,D-dimer level,and complication during perioperative period.Results Before surgery,there was no statistically significant difference on coagulation indexes between two groups.After 3 d of operation,there was no statistically significant difference in Plt,APTT,and PT between two groups.The FIB level of aspirin group was improved significantly and higher than that of rivaroxaban group (P ＜ 0.05).While the FIB level of rivaroxaban group was close to the normal level.Before operation,there was no statistically significant difference in D-dimer between two groups.After 1,3,and 7 d of operation,the D-dimer levels in two groups were increased.But the D-dimer levels of 3 and 7 d after operation began to decline.After 1,3,and 7 d of operation,the D-dimer ofrivaroxaban group was lower than that of aspirin group (P ＜ 0.05).During perioperative period,there was no statistically significant difference on DVT between two groups.Conclusion Rivaroxaban could prevent the formation of DVT effectively and reduce the D-dimer with good anticoagulation and high safety.It is worthy of clinical application.

Objective Jizhipingchuan soft capsula is mainly used to treat acute bronchitis and acute attack of chronic bron-chitis.This paper investigate the antitussive, antiasthmatic and expectorant effects of Jizhipingchuan soft capsula according to its func-tions. Methods The antitussive effect of Jizhipingchuan soft capsula was observed in cough mouse model induced by ammonia and in guinea cough pig model induced by citric acid, and the latent period of asthma was observed in guinea pig induced by acetylcholine chloride and histamine phosphate.Its expectorant effect was evaluated by the excretion of phenol red in mice and the exudation phlegm in rats. Results Jizhipingchuan soft capsula could decrease the frequence of cough induced by ammonia in mice and induced by cit-ric acid in guinea pig.It could prolong the latent period of cough in guine pig and also prolong the latent period of asthma in guine pig induced by acetylcholine chloride and histamine phosphate.Jizhipingchuan soft capsula could significantly increase the amount of phe-nol red secreted in mice and the phlegm exudation of rats. Conclusion Jizhipingchuan soft capsula showed definite antitussive, an-tiasthmatic and expectorant effects.

Objective To evaluate the necessity and feasibility of low-dose dual-source CT coronary angiography imaging in the evaluation of high body mass index (BMI) patients. Methods Sixty patients with BMI≥28 kg/m2 and heart rate 65-90 beats/min were selected, and they were divided into prospective electrocardiography gated scanning group and retrospective electrocardiography gated scanning group by random digits table method with 30 cases each. The image quality, CT dose weighing index (CTDI) and dose length product (DLP) were compared between 2 groups. Results There was no statistical difference in the score of coronary image quality between retrospective electrocardiography gated scanning group and prospective electrocardiography gated scanning group:(3.47±0.61) scores vs. (3.18±0.94) scores, P>0.05. The CTDI and DLP in prospective electrocardiography gated scanning were significantly lower than those that in retrospective electrocardiography gated scanning: (32.44 ±10.18) mGy vs. (45.86 ±15.34) mGy and (458.00±95.27) mGy/cm vs. (532.15±154.43) mGy/cm, and there were statistical differences (P<0.05). Conclusion For high BMI patients, different low-dose dual-source CT scanning, especially prospective electrocardiography gated scanning, can guarantee image quality and reduce the radiation dose and the radiation damage at the same time.

Objective To discuss the prenatal ultrasound manifestations of Apert syndrome. Methods The prenatal ultrasound features of 4 fetuses identified as Apert syndrome after detailed antenatal ultrasound examinations between January, 2010 to Februry, 2014 in our hospital were analyzed. The ultrasound images and postnatal outcome or autopsy were compared to analysis the sonographic features of Apert syndrome in prenatal. Results The prenatal ultrasound characteristics and pregnant outcome of the 4 fetuses were showed as followings:(1) Skull deformity:4 fetuses were all appeared as acrocephaly, coronal suture premature close and frontal protrusion, while 3 cases (case 1-3) had“clover leaf skull deformity”features. (2) Midfacial malformation deformity:3 cases (case 1-3) were all with hypertelorism and mid-sagittal facial proifle abnormal and only 1 case (case 2) had nose hypoplasia. (3) Limbs abnormal:4 cases were all appeared as bilateral symmetry syndactyly of hands and 1 case (case 2) was diagnosed as bilateral symmetry syndactyly of feet. (4) Associated abnormality:persistent right umbilical vein in 1 case (case1), thoracic spine stenosis in 1 case (case 2), hyperechogenic renal parenchyma in 1 case (case 3) and left-diaphragmatic hernia in 1 case (case 4). (5) The pregnant outcome:3 cases underwent pregnancy termination and 1 case was labored at term. The 3 cases (case1-3) were diagnosed as bilateral symmetry syndactyly of feet after induced labor. The cleft palate and bilateral symmetry syndactyly of feet were misdiagnosed in the full-time infant (case 3), who was died two days after birth. Conclusions The Apert syndrome is a rare syndrome. It generally has typical characteristic of prenatal ultrasound, such as coronal suture premature close, acrocephaly, mid-sagittal facial profile abnormal and bilateral symmetry syndactyly of hands and feet. Prenatal diagnosis of Apert syndrome can play an important role in genetic counseling and postnatal treatment.

Aim To study the effect of chromomycin on MPP~+-induced dopaminergic neuronal apoptosis.Methods An apoptosis model induced by 1-methyl-4-phenylpridnium ion(MPP~+)was established in cultured fetal mesencephalic neurons in vitro.Dopaminergic neuronal apoptosis was detected by Hoechst 33258 staining and phospho-Tau levels were detected by immunofluorescence.Results 10 μmol·L~(-1) MPP~+ hyperphosphorylated Tau at Ser396 and induced dopaminergic neuronal apoptosis.Chromomycin increased the number of TH-positive cells via inhibiting Tau phosphorylation.Conclusion These results indicate thatchromomycin inhibits Tau phosphorylation at Ser396 and therefore protects dopaminergic mesencephalic neurons from apoptosis induced by MPP+,which suggests that Chromomycin may be used to cure neurodegenerative disorders such as Parkinson's disease in clinic.

Objective:To investigate the effect of survivin-siRNA plasmid on survivin expression in human lung cancer cell line A549, and to observe its effect on the apoptosis, proliferation, and chemosensitivity of A549 cells. Methods: pSilencer-survivin-siRNA (survivin-siRNA) plasmid was constructed using pSilencer-U6 plasmid and was transfected into A549 cells. Expression of survivin mRNA and protein was examined by RT-PCR and Western blotting analysis, respec-tively. Apoptosis and proliferation of A549 cells were examined by DAPI staining and MTT, respectively. Results: Sur-vivin-siRNA plasmid was successfully constructed, and it significantly inhibited survivin mRNA and protein expression in A549 cells. Survivin-siRNA transfection induced apoptosis, inhibited proliferation and increased chemosensitivity of A549 cells to cisplatin. Conclusion: pSilencer-survivin-siRNA can silence survivin expression in A549 cells and subsequently inhibit proliferation, promote apoptosis, and enhance chemosensitivity of A549 cells to cisplatin. Survivin may serve as a potential target for gene therapy of lung cancer.

BACKGROUND:Pneumonectomy, extracorporeal circulation, lung transplantation and pulmonary embolism can cause ischemia/reperfusion injury of lung tissue. Lung ischemia/reperfusion injury is an important factor of lung function disorders after lung transplantation. OBJECTIVE:To analyze the effect of ischemic postconditioning on rat models of lung ischemia/reperfusion injury. METHODS:Twenty-four male SD rats were randomly divided into sham, ischemia/reperfusion and ischemic postconditioning groups (n = 8 rats/group) to establish the lung ischemia/reperfusion injury model. The rats in the sham group were only subjected to separation of the hilum of left lung and pulmonary arteries and veins, without blocking. The rats in the ischemia/reperfusion group were subjected to another 2 hours of reperfusion after 1 hour of lung ischemia. The rats in the ischemic postconditioning group were first subjected to 30 seconds of lung ischemia and 30 seconds of reperfusion for three times, and then to 2 hours of reperfusion. After the experiment, the specimens of lung tissue were obtained to detect the wet/dry weight ratio of lung tissue, activities of superoxide dismutase, malondialdehyde and myeloperoxidase, the contentsof inflammatory cytokines tumor necrosis factor α, interleukin-1β and interleukin-6, and the histopathological changes of lung tissue were observed. RESULTS AND CONCLUSION:Compared with the sham group, the wet/dry weight ratio of lung tissue, activities of myeloperoxidase and myeloperoxidase, the levels of tumor necrosis factor α, interleukin-1β and interleukin-6 were significantly increased (P < 0.05) in the ischemia/reperfusion and ischemic postconditioning groups, however, the increase levels of these indices were not significant in the ischemic postconditioning group, and the contents and activities in the ischemic postconditioning group were al significantly decreased (P < 0.05) compared with those in the ischemia/reperfusion group. In the ischemia/reperfusion and ischemic postconditioning groups, the activity of superoxide dismutase was obviously lower than that in the sham group, however, the activity of superoxide dismutase in the ischemic postconditioning group was obviously higher than that in the ischemia/reperfusion group. Pathological examination showed that thickened alveolar wal, edema and a large amont of inflammatory cel infiltrations were observed in the lung tissue of rats in the ischemia/reperfusion group. The degrees of alveolar wal thickening and edema in the lung tissue of rats in the ischemic postconditioning group were mild compared with the ischemia/reperfusion group, and in addition, some inflammatory cels were infiltrated. The histopathological scores of lung tissue in the ischemic postconditioning group were lower than those in the ischemia/reperfusion group. These results suggest that ischemic postconditioning plays its protective role on rat models of ischemia/reperfusion injury by inhibiting inflammatory cel accumulation, oxygen free radical production and pro-inflammatory cytokine release after ischemia/reperfusion injury.