Objective To investigate the effect and recurrence of the esomeprazole combined with trimebutine on treatment for non-erosive reflux disease(NERD).Methods One hundred and twenty-five cases of patients with NERD were randomly divided into the treatment group (n =62) and the control group (n =63).Patients in treatment group were received the esomeprazole 20 mg,twice a day and trimebutine 0.2 g,3 times a day,in control group were received the esomeprazole 20 mg,twice a day and mosapride 5 mg,3 times a day.After 8 weeks treatment,6 months follow-up was conducted and the effects and recurrence were evaluated.Results The clinical curative rates at 4th and 8th weeks treatment in treatment group were 75.8％ (47/62) and 95.2％ (59/62),higher than that of control group (57.1％ (36/63),x2 =4.879,P =0.027 ; 84.1％ (53/63),x2 =4.083,P =0.043).The GERDQ curative rates at 4th and 8th weeks treatment in treatment group were 72.6％ (45/62),93.5％ (58/62) respectively,significantly higher than that of the control group (52.4％ (33/ 63),x2 =5.434,P =0.020 ; 79.4％ (50/63),x2 =5.350,P =0.021).The recurrence rates of 6 months followup were 77.4％ (48/62) in the treatment group and 81.0％ (51/63) in the control group,there was no significant difference between the two groups (P =0.627).Conclusion Esomeprazole combined with trimebutine is safe and effective treatment on non-erosive reflux disease,and the recurrence rates was lower than that in the control group.

Objective To study the feasibility of dual-source CT with dual-energy imaging based on single contrast enhancement. Methods The clinical data of 60 cases of patients who underwent dual-energy CT (DECT)examination of the heart in our hospital were analyzed.statistically.Results The success rate of 60 cases were 100% for displaying all 600 coronary artery segments at all stages.The total mean score of image quality was 4.68 ±0.57,and the good image rate reached 95%.6 cases of patients who ac-cepted DSA examination were unanimous with DECT findings;In 6 cases of patients who accepted SPECT examination,60 segments were no perfusion abnormalities,but DECT iodine figures of 54 segments were normal,and 6 segments showed perfusion defect. Conclusion DECT with dual-energy imaging based on single contrast enhancement can get excellent coronary and myocardial perfu-sion imaging in the circumstance of appropriate heart rate,which has some potentials for clinical application.

Objective:To explore the effect of the intervention model based on the theory of knowledge, belief and action on the prevention of dislocation of prosthesis after hip replacement by nurses, so as to provide guidance for the management of prosthesis dislocation prevention after hip replacement.Methods:The patients were from Shenzhen Luohu People′s Hospital. From January to March 2018, 82 patients who underwent artificial total hip arthroplasty were set as the control group and received routine care. The 76 patients who underwent artificial total hip arthroplasty from May to July 2018 were set as the observation group, and the intervention model based on the theory of knowledge, belief and action was adopted. The incidence of prosthesis dislocation was compared between the two groups at 1, 3, 6 months after operation. At the same time, the Harris hip function score and the activity of daily living (ADL) score were compared between the two groups at the time of discharge, 1, 3, 6 months after operation.Results:The total incidence of prosthesis dislocation at 6 months was 1.32% (1/76) and 9.76% (8/82) in the experimental group and the control group, respectively, and the difference was statistically significant ( χ2=5.23, P<0.05). At discharge, there was no significant difference in the Harris hip function score and ADL scores between the two groups ( P>0.05). The Harris hip function score and ADL scores at 1, 3, 6 months after operation were (80.05 ± 6.72), (88.76 ± 5.84), (94.07 ± 4.43) points and (55.72 ± 16.61), (63.55 ± 14.55), (75.39 ± 12.27) points in the observation group, and (74.41 ± 6.26), (84.48±5.97), (89.30 ± 5.32) points and (47.13 ± 19.77), (56.71 ± 16.01), (63.96 ± 13.78) points in the control group, the differences were statistically significant ( t values were -6.08--2.80, all P<0.05). Conclusions:The intervention model based on the theory of knowledge, belief and action can effectively improve the Harris hip function score and ADL score of patients at 1, 3, 6 months after hip replacement, and reduce the incidence of prosthetic dislocation.

Objective:To develop the Orthopedic Nurses' Prevention of Prosthesis Dislocation after Total Hip Arthroplasty Scale based on the knowledge, attitude and practice model, and to test its reliability and validity.Methods:Guided by the theory of knowledge, attitude and practice, on the basis of the establishment of a research group, literature review, expert consultation and pre-investigation, the Orthopedic Nurses' Knowledge, Attitude and Practice Scale for Prevention of Prosthesis Dislocation after Total Hip Arthroplasty was formed. From October 16, 2019 to March 15, 2020, 272 orthopedic nurses from 15 hospitals in Shenzhen were selected for survey by convenience sampling. Exploratory factor analysis, internal consistency test and other methods were used to test the reliability and validity of the scale.Results:The Orthopedic Nurses' Knowledge, Attitude and Practice Scale for Prevention of Prosthesis Dislocation after Total Hip Arthroplasty included 3 dimensions of knowledge, attitude and practice, with a total of 32 items. Four common factors were extracted by exploratory factor analysis, and the cumulative variance contribution rate was 57.206%. The Cronbach's α coefficients of the three dimensions of knowledge, attitude, and practice were 0.976, 0.897, and 0.956, the test-retest reliability coefficients were 0.701, 0.852 and 0.903, and the content validity index were 0.89, 0.91 and 0.93, respectively.Conclusions:The Orthopedic Nurses' Knowledge, Attitude and Practice Scale for Prevention of Prosthesis Dislocation after Total Hip Arthroplasty has good reliability and validity, and can be used as an evaluation tool for nurses' knowledge, attitude, and practice survey on prosthesis dislocation after total hip arthroplasty.

OBJECTIVETo investigate the effect of macrophages on embryo implantation by observing the distribution of macrophages in mouse uterine tissues during the peri-implantation period.

METHODSUterine tissues were collected from pregnant (n=30) and pseudopregnant mice (n=30) during the peri-implantation period. The distributions of macrophages, iNOS and leukemia inhibitory factor (LIF) were determined by immunohistochemistry and the correlations of macrophages with iNOS and LIF were analyzed.

RESULTSMacrophages were located mainly in the endometrium before D4.5 in the pregnant rats with D0.5 defined as the morning when a vaginal plug was observed. After D4.5, the macrophages was significantly reduced in number (P<0.05) in the endometrium and gradually migrated to the perimetrium. In the psudopregnant mice, macrophages were located mainly in the endometrium. Before D4.5, iNOS-positive cells were detected mainly in the endometrium and the myometrium in the pregnant rats and became significantly reduced on D4.5 (P<0.05); in the pseudopregnant mice, the positive cells were mostly detected in the endometrium. Significant differences were found in the distribution of the macrophages and LIF between the implantation and non-implantation sites (P=0.013). LIF was mostly located in the endometrium in the pregnant mice but scarcely detected in the pseudopregnant mice.

CONCLUSIONMacrophages are located mainly in the endometrium and the implantation site where iNOS and LIF are expressed, suggesting the important role of macrophages in the determination of implantation.

Animals ; Blood Cell Count ; Embryo Implantation ; Endometrium ; cytology ; Female ; Immunohistochemistry ; Leukemia Inhibitory Factor ; metabolism ; Macrophages ; cytology ; Mice ; Nitric Oxide Synthase Type II ; metabolism ; Pregnancy ; Uterus ; cytology

Objective:To investigate the expression level of poly ADP ribose polymerase 14(PARP14) in thyroid cancer and its relationship with the clinicopathologic characteristics of the patient with thyroid cancer and evaluate the role of PARP14 in the progression of thyroid cancer.Methods:The gene expression interaction analysis (GEPIA) database was used to analyze the expression of PARP14 in normal thyroid tissue and thyroid cancer tissue and its relationship with disease-free survival of patients. The expression of PARP14 in thyroid cancer tissue and adjacent tissues of the patient with thyroid cancer was evaluated by immunohistochemistry. According to the staining intensity, the patients were divided into the high expression group and the low expression group, and the correlation between the expression of PARP14 and clinical pathological characteristics was analyzed. The effect of PARP14 on the proliferation of thyroid cancer cells was investigated by clone formation testing and MTT testing.Results:The results of bioinformatics analysis and immunohistochemistry showed that PARP14 was overexpressed in thyroid cancer tissue, and the disease-free survival rate of the patient with high expression was lower. The expression level of PARP14 was correlated with tumor stage and intrathyroidal spread (all P<0.05). The results of the clonogenic assay and the MTT assay showed that the expression of KIF4A could promote the proliferation of thyroid cancer cells ( P<0.05). Conclusions:PARP14 is highly expressed in thyroid cancer and is related to the clinicopathological characteristics of patients, suggesting that it may be a therapeutic target for thyroid cancer.

Objective To investigate the effect of macrophages on embryo implantation by observing the distribution of macrophages in mouse uterine tissues during the peri-implantation period. Methods Uterine tissues were collected from pregnant (n=30) and pseudopregnant mice (n=30) during the peri-implantation period. The distributions of macrophages, iNOS and leukemia inhibitory factor (LIF) were determined by immunohistochemistry and the correlations of macrophages with iNOS and LIF were analyzed. Results Macrophages were located mainly in the endometrium before D4.5 in the pregnant rats with D0.5 defined as the morning when a vaginal plug was observed. After D4.5, the macrophages was significantly reduced in number (P<0.05) in the endometrium and gradually migrated to the perimetrium. In the psudopregnant mice, macrophages were located mainly in the endometrium. Before D4.5, iNOS-positive cells were detected mainly in the endometrium and the myometrium in the pregnant rats and became significantly reduced on D4.5 (P<0.05); in the pseudopregnant mice, the positive cells were mostly detected in the endometrium. Significant differences were found in the distribution of the macrophages and LIF between the implantation and non-implantation sites (P=0.013). LIF was mostly located in the endometrium in the pregnant mice but scarcely detected in the pseudopregnant mice. Conclusion Macrophages are located mainly in the endometrium and the implantation site where iNOS and LIF are expressed, suggesting the important role of macrophages in the determination of implantation.

Objective To investigate the effect of macrophages on embryo implantation by observing the distribution of macrophages in mouse uterine tissues during the peri-implantation period. Methods Uterine tissues were collected from pregnant (n=30) and pseudopregnant mice (n=30) during the peri-implantation period. The distributions of macrophages, iNOS and leukemia inhibitory factor (LIF) were determined by immunohistochemistry and the correlations of macrophages with iNOS and LIF were analyzed. Results Macrophages were located mainly in the endometrium before D4.5 in the pregnant rats with D0.5 defined as the morning when a vaginal plug was observed. After D4.5, the macrophages was significantly reduced in number (P<0.05) in the endometrium and gradually migrated to the perimetrium. In the psudopregnant mice, macrophages were located mainly in the endometrium. Before D4.5, iNOS-positive cells were detected mainly in the endometrium and the myometrium in the pregnant rats and became significantly reduced on D4.5 (P<0.05); in the pseudopregnant mice, the positive cells were mostly detected in the endometrium. Significant differences were found in the distribution of the macrophages and LIF between the implantation and non-implantation sites (P=0.013). LIF was mostly located in the endometrium in the pregnant mice but scarcely detected in the pseudopregnant mice. Conclusion Macrophages are located mainly in the endometrium and the implantation site where iNOS and LIF are expressed, suggesting the important role of macrophages in the determination of implantation.

ObjectiveTo establish the specific chromatogram and thin layer chromatography（TLC） of Qingxin Lianziyin（QXLZY） benchmark samples， in order to clarify the key quality attributes and provide a reference for the quality evaluation of QXLZY. MethodHigh performance liquid chromatography（HPLC） specific chromatogram of QXLZY benchmark samples was developed by using a YMC Hydrosphere C₁₈ column（4.6 mm×250 mm， 5 μm） with the mobile phase of acetonitrile（A）-0.2% formic acid aqueous solution（B） for gradient elution（0-10 min， 5%-20%A； 10-20 min， 20%A； 20-25 min， 20%-24%A； 25-40 min， 24%-30%A； 40-55 min， 30%-50%A； 55-65 min， 50%-100%A； 65-75 min， 100%A； 75-75.1 min， 100%-5%A； 75.1-90 min， 5%A）， and the detection wavelength was 360 nm. Ultra-high performance liquid chromatography-linear ion trap/orbitrap mass spectrometry（UHPLC-LTQ-Orbitrap MS） with electrospray ionization（ESI） was used to identify the components of QXLZY benchmark samples by accurate relative molecular weight and multilevel MS fragment ion information， the detection conditions were positive and negative ion modes and data dependency scanning mode. TLC identification methods for Ophiopogonis Radix， Lycii Cortex， Nelumbinis Semen， Poria， Astragali Radix and Ginseng Radix et Rhizoma in QXLZY were established. ResultA total of 15 characteristic peaks were identified from Glycyrrhizae Radix et Rhizoma， Plantaginis Semen and Scutellariae Radix， and the relative standard deviations of the retention times of 15 characteristic peaks in 15 batches of QXLZY benchmark samples were≤3% with peak 8（baicalin） as the reference peak. A total of 100 compounds， including flavonoids， organic acids， saponins， amino acids and others， were identified in the benchmark samples by UHPLC-LTQ-Orbitrap MS. The established TLC had good separation and was suitable for the identification of Ophiopogonis Radix， Lycii Cortex， Nelumbinis Semen， Poria， Astragali Radix and Ginseng Radix et Rhizoma in QXLZY. ConclusionThe material basis of QXLZY benchmark samples is basically determined by MS designation and source attribution. The established specific chromatogram and TLC of QXLZY are simple， stable and reproducible， which can provide a reference for the development and quality control of QXLZY.

This paper aims to study the accuracy of cardiopulmonary physiological parameters measurement under different exercise intensity in the accompanying (wearable) physiological parameter monitoring system. SensEcho, an accompanying physiological parameter monitoring system, and CORTEX METALYZER 3B, a cardiopulmonary function testing system, were used to simultaneously collect the cardiopulmonary physiological parameters of 28 healthy volunteers (17 males and 11 females) in various exercise states, such as standing, lying down and Bruce treadmill exercise. Bland-Altman analysis, correlation analysis and other methods, from the perspective of group and individual, were used to contrast and analyze the two types of equipment to measure parameters of heart rate and breathing rate. The results of group analysis showed that the heart rate and respiratory rate data box charts collected by the two devices were highly consistent. The heart rate difference was (-0.407 ± 3.380) times/min, and the respiratory rate difference was (-0.560 ± 7.047) times/min. The difference was very small. The Bland-Altman plot of the heart rate and respiratory rate in each experimental stage showed that the proportion of mean ± 2SD was 96.86% and 95.29%, respectively. The results of individual analysis showed that the correlation coefficients of the whole-process heart rate and respiratory rate data were all greater than 0.9. In conclusion, SensEcho, as an accompanying physiological parameter monitoring system, can accurately measure the human heart rate, respiration rate and other key cardiopulmonary physiological parameters under various sports conditions. It can maintain good stability under various sports conditions and meet the requirements of continuous physiological signal collection and analysis application under sports conditions.