Allergen specific immunotherapy(AIT) is a desensitization treatment for IgE-mediated allergic diseases.Combined with pharmacological therapy,AIT is used to treat allergic asthma,allergic rhinitis and atopic dermatitis caused by allergens that cannot be avoided.AIT is the only treatment modality that can alter the natural course of allergic disease.However,because of the lack of studies,there still exists controversy about the application of immunotherapy in children with allergic diseases.The mechanism ofAIT,its clinical efficacy and safety in children with allergic diseases are reviewed.

Objective To observe the effect of oxygen treatment plus non-invasive positive pressure ventilation on the stabillzation of chronic obstractive pulmonary disease and the influence on exercise tolerance.Methods The blood gas anylisis of and the result of 6 min walk test were observed and compared with self-control method.Results There was significant difference between the beginning and termination of the treatment in 10 patients haying finished the treatment and the results of 6 min walk test(P＜0.05).Conclusion The oxygen treatment plus non-invasive positive pressure ventilation in the stabilization of chronic obstructive pulmonary disease is safe and effective.

Objective To investigate the relationship between TCM syndrome of hyperlipidemia and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Methods 200 patients of hyperlipidemia recruited from the cadre ward in hospital were made TCM syndrome differentiation according to Guiding principles for the clinical study of Chinese Medicines.SPSS16.0 statistical software were adopted for analyzing the relation between TCM syndrome and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Results ①)There are no differences TCM syndrome and the indexes of age,sex,TC,TG,HDL-C,LDL-C,VLDL-C,lipoproteins,the vascular media,coagulation and inflammatory indices.②Compared with the liver kidney yin deficiency syndrome group,apolipoprotein A was higher in the groups of qi stagnation and blood stasis,phlegm stagnation syndrome,and hyperactivity of Yang due to yin deficiency syndrome,the difference was statistically significant; while APOB showed no difference among the groups.Conclusion TCM syndrome of hyperlipidemia had no relation to indexes of age,sex,body mass index,blood lipid,and blood coagulation.The study found a correlation between hyperlipidemia and phlegm and Qi deficiency,which can provide a theoretical basis for syndrome differentiation of TCM.

Objective: To study the relationship between maternal behavior and behavior problem of their children Method: 765 mothers with children aged 6 to 11 were assessed with MBQ (maternal behavior questionnaire) and behaviors of their children were assessed with CBCL (Achenbach children's behavior checklist) Results: 12 0% of children was identified as having behavior problems Maternal supportive behavior decreased behavior problems of their children, while maternal un-supportive behavior increased that Conclusion: Maternal behaviors had a significant effect on children's behavior problem

Objective To study the clinical characteristics of the severe acute respiratory syndrome(SARS) patients who progressed into multiple organ injury Methods Retrospective analysis was performed on the clinical data of 54 severe SARS patients with multiple organ injury in our hospital Results In 54 SARS patients,systemic inflammatory response syndrome(SIRS) developed in all cases and multiple organ injury developed in 49 cases and the mortality of patients with multiple organ injury was 22 2% Among the involved organs,lungs were the most frequently involved organ,followed by liver,heart,brain,kidney and blood system The injury in other organs was rare Conclusions Serious SARS is predisposed to progress into multiple organ injury The mortality was correlated to the number of organs involved Recognization and treatment of multiple organ injury at early stage are critical with improve cure rate of serious SARS with multiple organ injury

A total of 35 patients with hallus valgus (56 feet) received treatment at the Department of Orthopedics, the Sixth People's Hospital, Shanghai Jiao Tong University between January 2004 and June 2005 and were recruited into this study. These patients underwent osteotomy of proximal first metatarsal and internal fixation with poly (L-lactic acid) (PLLA) absorbable pins. All patients were followed up for 4-22 months. Excellent outcomes were found in 27 patients (44 feet) and good results in 8 patients (12 feet). The mean healing time of surgical region was 6 weeks, nonunion or delayed union was not found. There were 3 cases suffering from complications post surgery. Two of them presented with peripheral wound red swelling, considering as a result of extraneous material-caused aseptic inflammatory reaction. This phenomenon would be regressed 2-4 weeks after hydropathic compress of ethanol gauzes and timely renewal of dressings. There was 1 case who suffered from protrution of absorbable pin 1 week after fixation and received reposition and plaster fixation. Bone displacement and nonunion were not found. This complication maybe results from excessively large bone pore due to shaking of electrodrill, which leads to loosening of absorbable pins.

BACKGROUND: It is important to study the methods of culturing bone marrow-derived mesenchymal stem cells (MSCs) to obtain a great amount of high purity MSCs for applying ocular tissues constructed by tissue engineering technique to treat eye diseases.OBJECTTVE: To separate and culture in vitro MSCs from bone marrow of the adult rats by density gradient centrifugation combined with adherence culture, and observe the growing characteristics and the possibility of mass multiplication.DESIGN: A completely randomized grouping design/repetitive measuring experiment.SETTING: Pathological laboratory, Zhongshan Ophthalmic Center, Sun Yat-sen University.MATERIALS: Four six-week-old SD rats about 250 g, grade Ⅱ of cleaning, were provided by the Animal Center of Sun Yat-sen University [certificate number: SCSK(Yue)2004/0011], about 250 g each rat and there was no limit to the sex. The main reagents and instruments included low sugar Dulbecco modified Eagle culture medium (DMEM/F12, American Gibco Corporation), trypsin (fetal bovine serum (FBS, Hangzhou Sijiqing Bio-Engineering Material Research Institute), American Gibco Corporation), disodium edetate, lymphocyte separating medium, fibronectin, CD44, CD34, CD31 monoclonal antibodies, two-step-method kit for immunohistochemistry (Beijing Zhong shan Biotechnology Corporation).METHODS: This experiment was conducted at the Key laboratory of Ophthalmology (Sun Yat-sen University), Ministry of ethanol (750 g/L) for 10 minutes. Under aseptic condition, the medullaris cavitas was exposed, the syringe containing application m edium was directly punctured into the femoral cavity, the cells in the medullaris cavitas were washed out with the culture medium containing heparin and taken as the cell suspension. The bone marrow-derived MSCs were separated and purified by density gradient centrifugation combined with adherence culture, and the growing conditions of the wells. When the cells had generally connected with each other, they were fixed with methanol or dimethy ketone in situ for 10 minutes, and then hematoxylin eosin (HE) staining or immunohistochemical staining. Antibodies against fiinoculated to 96-well culture plate by a cell density of 4.25×107/L, with 200 μL every well; then put the culture plate into culture box. Then from the next day to the sixth day, 5 g/L MTT solution was added into two rows (20 μL every well) every day, continuously cultured for 4 hours, then the supernatant was removed, and 200 μL DMSO was added to each well, agitated for 5 minutes, then detected the absorbance (A) values at 570 nm wave length, and a growth curve was drawn.branes were clear and the cell bodies were lucent. Being cultured for 2 days, there appeared adherent cells. 3 days later,most of the adherent cells extended and appeared to be in polygon, star or long-shuttle shapes. 4 days later, the cells showed to be in division growth stage; and about 12 days later, the cell clones were connected to each other, appearing curve of subcultured MSCs was in S shape. Cells began growing fast on the 2nd day after being passaged, and they entered the growth period for 3-4 days followed by saturation period, and then cells stopped growing.CONCLUSION: It is a simple and practical method to separate MSCs from the bone marrow of adult rats by means of density gradient centrifugation and adherence. MSCs can greatly proliferate in vitro and offer seed cells for the application of tissue engineering technique to treat eye diseases.

OBJECTIVE:To optimize water extraction technology of Shenqi yiqi capsules. METHODS:The water extraction technology of Shenqi yiqi capsules was optimized by L9(34)orthogonal test with amount of water added,decoction times and decoc-tion time as factors using the extraction rate of astragaloside,calyosin-7-O-β-D-glycoside,icariin and bergenin as index,and the verification test was made. RESULTS:The best extraction technology was as follows as 8-fold water,decocting for 3 times,2 h each time;comprehensive score of 3 batches of samples in validation test were 98.95,99.71 and 99.98(RSD<2%,n=3). CON-CLUSIONS:The water extraction technology optimized by multi-index comprehensive score-orthogonal test is stable,scientific and reasonable.

Objective To clone the human bone morphogenetic protein-2(hBMP2) gene cDNA and construct its replication-deficient type 5 adenoviral vector.Methods Using osteoblastic sarcoma tissue to extract cell total RNA,hBMP-2 cDNA was amplified by RT-PCR.The hBMP-2 cDNA was then inserted into cloning vector pGEM-T.The recombinant plasmid was screened and the recombinant plasmid which was confirmed by restriction enzyme,PCR and sequence analysis was gotten.To construct the recombinant adenoviral vector,the BMP-2 cDNA was subcloned into a shuttle vector and the recombinant pShuttle-BMP2 vector was obtained.It was subcloned into Adeno-X adenoviral genomic DNA and the pAd-BMP2 DNA was obtained.Results A length of 1 200 bp BMP-2 cDNA was cloned,and it was subcloned into adenoviral vector,the recombinant adenovirus was constructed successfully.After measuring the titre of virus(1?1011 nfu?L-1),the target gene was evaluated by PCR.Conclusion The hBMP-2 adenoviral is obtained.The successful construction of pAd-BMP2 may provide us useful target for the further research.

Endometrial Neoplasms ; drug therapy ; pathology ; Female ; Humans ; Precancerous Conditions ; drug therapy ; pathology ; Progestins ; therapeutic use