Aim To investigate the differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide(DATS).Methods Growth inhibition against MGC803 cells was assayed by MTT assay;The apoptosis induced by DATS was assessed by Flow cytometry and fluorescent microscope.The apoptosis-associated gene expression of MGC803 cell treated with DATS was determided by Human Apoptosis Gene Array.Apaf-1 and SODD genes were confirmed by RT-PCR.Results DATS had significant growth inhibitory activity against MGC803 cells,inhibition ratio increased from 11% to 78% at 4,8,12,16 and 24 mg?L-1 for 72 h(P

OBJECTIVETo investigate the effect of tumor necrosis factor-α (TNF-α) on nutritional status and proteolysis of respiratory muscle in a rat model of chronic obstructive pulmonary disease (COPD).

METHODSNinety healthy male adult Wistar rats were randomly divided into model group (A) and normal control group (B). COPD malnutrition rat models were established by cigarettes smoke and nutrient limitation and divided into normal nutrition COPD group (A(1)), malnutrition COPD group (A(2)), and malnutrition COPD intervention group (A(3)). In group A(3), the rats received intravenous injection of TNF-α mAb (0.1 mg/kg). TNF-α levels in the serum and respiratory muscle homogenates were measured using enzyme-linked immunosorbent assay (ELISA), and plasma levels of glucose, albumin, and triglyceride were measured with an automatic biochemistry analyzer. High-performance liquid chromatography was used to measure the contents of 3-methylhistidine and tyrosine in the respiratory muscle homogenates.

RESULTSThe serum TNF-α level and plasma levels of glucose and triglyceride were significantly higher but the plasma albumin level was significantly lower in group A(2) than in groups B, A(1), and A(3) (P<0.01). The contents of 3-MH and Tyr in the respiratory muscle homogenates were significantly higher in group A(2) than in the other 3 groups (P<0.01, P<0.01). TNF-α in the respiratory muscle showed a strong positive correlation to 3-MH and Tyr.

CONCLUSIONTNF-α is one of the causes of increased proteolysis of the respiratory muscle.

Animals ; Lung ; pathology ; Male ; Nutritional Status ; Proteolysis ; Pulmonary Disease, Chronic Obstructive ; metabolism ; pathology ; Rats ; Respiratory Muscles ; metabolism ; Tobacco Products ; Tumor Necrosis Factor-alpha ; pharmacology

OBJECTIVETo observe the effect of Rgl treatment on prognosis of alcoholic hepatitis using a rat model.

METHODSFemale Sprague-Dawley rats were radomly divided into four groups:unmodeled control, untreated model, Rgl-treated model, and dexamethasone (DXM)-treated model. The model groups were generated by intragastric injection of alcohol. The unmodeled control group was given an equal dosage of normal saline by the same route. After model establishment, the Rg1 treatment group and the DXM treatment group were administered a 120-hour treatment of Rgl or DXM; the unmodeled controls were administered normal saline on the same schedule. All rats were then fasted for 120 hours and venous blood samples were collected for detection of serum aspartate aminotransferase (AST), alanine transaminase (ALT), total bilirubin (TBil), albumin (Alb), tumor necrosis factor-alpha (TNFat) and interleukin 6 (IL-6). Markers of liver inflammation were measured by immunohistochemistry, western blotting, and real-time quantitative reverse transcription PCR. Fat and apoptosis indices were assessed by hematoxylin-eosin staining and TUNEL assay, respectively. The t-test and F test were used for statistical analyses.

RESULTSThe model group showed remarkably more liver steatosis (over one-third of the tissue) than the unmodeled control group, indicating proper establishment of alcoholic liver disease in the modeled rats. The AST, ALT, TBil, and IL-6 levels were significantly higher in the untreated model group than in the Rgl-treated group and the DXM-treated group. The values were significantly different between the Rg1-treated group and the DXM-treated group:ALT, 69.19+/-8.00 U/L vs.102.88+/-5.16 U/L; TBil, 0.36+/-0.07 µmol/L vs.1.20+/-0.18 µmol/L; IL-6, 126.50+/-6.50 U/ml vs.169.19+/-7.68 U/ml; TNFa, 268.31+/-13.19 µg/L vs.318.94+/-7.87 µg/L (all P less than 0.05). Expression of caspase3 and caspase8 was significantly higher in the model group than in the Rgltreated group and the DXM-treated group (both P<0.05). The apoptosis index was significantly lower in the Rgltreated group and the DXM-treated group than in the model group (both P<0.05). The mRNA and protein expression of caspase3, caspase8 and NF-kB were significantly lower in the Rgl-treated group and the DXM-treated group than in the model group (allP less than 0.05), and the levels of all were significantly lower in the Rgl-treated group cornered to the DXM-treated group (all P<0.05). Conehision In rats with alcoholic hepatitis, Rg1 can significantly relieve pathological injury, improve liver function by blocking the apoptotic pathway, and inhibit release of inflammatory cytokines.

Alanine Transaminase ; Animals ; Aspartate Aminotransferases ; Bilirubin ; Cytokines ; Disease Models, Animal ; Ethanol ; Female ; Ginsenosides ; Hepatitis, Alcoholic ; NF-kappa B ; Rats ; Rats, Sprague-Dawley

OBJECTIVE: To explore the effect of different concentrations of vitamin C on proliferation and apoptosis of C(2)C(12) myoblasts. METHODS: C(2)C(12) cells cultured in vitro were collected by trypsinization to form monoplast suspension, and then centrifuged to float again. The cellular numbers were counted and the cell suspension density adjusted, and the cells were inoculated into 96-shadow mask according to 200 μL per hollow. All cells were cultured in the normal way. While cell fusion ratio arrived 80% and cells did not differentiate, cells were divided into 6 groups: a negative control group (pure DMEM-F12 medium), an H(2)O(2) group (DMEM-F12 medium containing 500 μmol/L H(2)O(2)) and vitamin C group 1 to 4(DMEM-F12 medium containing 500 μmol/L H(2)O(2) and 10, 20, 60, and 100 mg/L vitamin C, respectively). After each group was treated for 0, 6, 24, 36, 48,and 72 h, respectively, MTT was used to detect C(2)C(12) cell proliferation in each group. Annexin V-PI double staining was applied to detect C(2)C(12) cell apoptosis in each group after treatment for 36 h. RESULTS: After the cells were treated for 36 h and 72 h, the absorbance of vitamin C group 1 to 4 were higher than that of H(2)O(2) group (P<0.001). The absorbance of vitamin C group 4 was the highest among all the groups, significantly higher than that of the negative control group when the cells were treated for 36 h (P<0.05). When the cells were treated for 36 h, the C(2)C(12) cells apoptosis rate of vitamin C group 2 to 4 was lower than that of H(2)O(2) group; The C(2)C(12) cells apoptosis rate of vitamin C group 2 and 3 was higher than that of the negative control group, while the C(2)C(12) cells apoptosis rate of vitamin C group 4 was significantly lower than that of the negative control group (P=0.009). CONCLUSION Vitamin C can efficiently inhibit the apoptosis of C(2)C(12) myoblasts induced by H(2)O(2), and after 36 h intervention, high concentration vitamin C may promote C(2)C(12) the proliferation of myoblasts.
Antioxidants ; pharmacology ; Apoptosis ; drug effects ; Ascorbic Acid ; pharmacology ; Cell Line ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Humans ; Myoblasts ; cytology ; Oxidative Stress ; drug effects

Objective To establish a method for measuring blood phosphatidylethanol by liquid chromatography coupled with tandem mass spectrometry(LC-MS/MS), which can be applied for objective and quantitative of alcohol intake.Methods Whole blood samples were treated with isopropanol to precipitate protein,and phosphopropanol(16:0/16:0)was used as the standard.After centrifugation, the supernatants were transferred and evaporated under a stream of nitrogen until dryness.Then the residuals were analyzed by LC-MS/MS.Various methodological parameters, including linearity, limit of detection (LOD), limit of quantitation(LOQ), recovery, and precision, were investigated.Finally, blood samples from 40 Chinese individuals with more than one year of regular drinking habits were analyzed, and distributions of phosphatidylethanol were evaluated.Results The correlation coefficients were higher than 0.9992.The LOD and LOQ were lower than 0.74 and 2.48 ng/ml, respectively.The inter-and total assay coefficient of variations were 0.77%-3.18% and 2.30%-6.95%, respectively, with recoveries ranged from 96.88% to 102.99%.The relationship between phosphatidylethanol level and self-reported alcohol consumption was significantly and positively correlated(r =0.769, P <0.001).Furthermore, Kruskal-Wallis analysis showed a significant difference in total phosphatidylethanol levels among individuals with different levels of alcohol intake(χ2=18.850,P<0.001).Conclusions An LC-MS/MS method for whole blood phosphatidylethanol detection has been developed.This method is simple,sensitive and accurate and can effectively reflect light,moderate and heavy alcohol intake.The method will be applied to the assessment of alcohol consumption and its association with the risks of drinking related diseases.

Objective Using Disease Diagnosis Related Grouping(DRG)index to assess the professional compe-tence of physicians in the evaluation of health professional and technical titles,and analysing their effectiveness.Methods The DRG indexes of 1 184 physicians were statistically analyzed before and after the implementation of DRG promotion policy.After normal test,Wilcoxon rank sum test was used to compare the CMI,number of DRG groups,total weight,time consumption index and cost consumption index of 1184 physicians in a hospital before and after applying DRG index to assess individual ability.Results The study found that after the introduction of DRG in-dex,CMI,number of DRG groups and total weight of physicians all showed an increasing trend,in which CMI in-creased from 1.17 to 1.20,time consumption index decreased,cost consumption index had no statistical signifi-cance(P＞0.05),and the difficulty of hospital treatment and medical efficiency improved.Conclusion The use of DRG index to assess doctors'professional ability has a positive effect on hospitals,which can improve the quality of diagnosis and treatment,improve efficiency,optimize patient grouping and medical division.DRG index can be used as a tool to assess doctors'professional ability.

Objective Using Disease Diagnosis Related Grouping(DRG)index to assess the professional compe-tence of physicians in the evaluation of health professional and technical titles,and analysing their effectiveness.Methods The DRG indexes of 1 184 physicians were statistically analyzed before and after the implementation of DRG promotion policy.After normal test,Wilcoxon rank sum test was used to compare the CMI,number of DRG groups,total weight,time consumption index and cost consumption index of 1184 physicians in a hospital before and after applying DRG index to assess individual ability.Results The study found that after the introduction of DRG in-dex,CMI,number of DRG groups and total weight of physicians all showed an increasing trend,in which CMI in-creased from 1.17 to 1.20,time consumption index decreased,cost consumption index had no statistical signifi-cance(P＞0.05),and the difficulty of hospital treatment and medical efficiency improved.Conclusion The use of DRG index to assess doctors'professional ability has a positive effect on hospitals,which can improve the quality of diagnosis and treatment,improve efficiency,optimize patient grouping and medical division.DRG index can be used as a tool to assess doctors'professional ability.

Objective Using Disease Diagnosis Related Grouping(DRG)index to assess the professional compe-tence of physicians in the evaluation of health professional and technical titles,and analysing their effectiveness.Methods The DRG indexes of 1 184 physicians were statistically analyzed before and after the implementation of DRG promotion policy.After normal test,Wilcoxon rank sum test was used to compare the CMI,number of DRG groups,total weight,time consumption index and cost consumption index of 1184 physicians in a hospital before and after applying DRG index to assess individual ability.Results The study found that after the introduction of DRG in-dex,CMI,number of DRG groups and total weight of physicians all showed an increasing trend,in which CMI in-creased from 1.17 to 1.20,time consumption index decreased,cost consumption index had no statistical signifi-cance(P＞0.05),and the difficulty of hospital treatment and medical efficiency improved.Conclusion The use of DRG index to assess doctors'professional ability has a positive effect on hospitals,which can improve the quality of diagnosis and treatment,improve efficiency,optimize patient grouping and medical division.DRG index can be used as a tool to assess doctors'professional ability.

Objective Using Disease Diagnosis Related Grouping(DRG)index to assess the professional compe-tence of physicians in the evaluation of health professional and technical titles,and analysing their effectiveness.Methods The DRG indexes of 1 184 physicians were statistically analyzed before and after the implementation of DRG promotion policy.After normal test,Wilcoxon rank sum test was used to compare the CMI,number of DRG groups,total weight,time consumption index and cost consumption index of 1184 physicians in a hospital before and after applying DRG index to assess individual ability.Results The study found that after the introduction of DRG in-dex,CMI,number of DRG groups and total weight of physicians all showed an increasing trend,in which CMI in-creased from 1.17 to 1.20,time consumption index decreased,cost consumption index had no statistical signifi-cance(P＞0.05),and the difficulty of hospital treatment and medical efficiency improved.Conclusion The use of DRG index to assess doctors'professional ability has a positive effect on hospitals,which can improve the quality of diagnosis and treatment,improve efficiency,optimize patient grouping and medical division.DRG index can be used as a tool to assess doctors'professional ability.

Objective Using Disease Diagnosis Related Grouping(DRG)index to assess the professional compe-tence of physicians in the evaluation of health professional and technical titles,and analysing their effectiveness.Methods The DRG indexes of 1 184 physicians were statistically analyzed before and after the implementation of DRG promotion policy.After normal test,Wilcoxon rank sum test was used to compare the CMI,number of DRG groups,total weight,time consumption index and cost consumption index of 1184 physicians in a hospital before and after applying DRG index to assess individual ability.Results The study found that after the introduction of DRG in-dex,CMI,number of DRG groups and total weight of physicians all showed an increasing trend,in which CMI in-creased from 1.17 to 1.20,time consumption index decreased,cost consumption index had no statistical signifi-cance(P＞0.05),and the difficulty of hospital treatment and medical efficiency improved.Conclusion The use of DRG index to assess doctors'professional ability has a positive effect on hospitals,which can improve the quality of diagnosis and treatment,improve efficiency,optimize patient grouping and medical division.DRG index can be used as a tool to assess doctors'professional ability.