Objective To prepare the mB7-1-GPI-anchored Lewis vaccine and investigate its antitumor effects. Methods mB7-1-GPI was incorporated on Lewis tumor cells and mB7-1-GPI-anchoring tumor vaccine was prepared. The anti-tumor immunity induced by the prepared mB7-1-GPI-anchored Lewis tumor cell vaccine in tumor-bearing mice was observed. Results Flow cytometric analysis showed that mB7-1-GPI were positively expressed on the surface of Lewis tumor cells. After Lewis tumor cells incubated with mB7-1-GPI, the positive rate (PR) of mB7-1 antigen was 95.8% (0h), 93.6% (4h), 91.1% (8h) and the fluorescence intensity (FI) was 11.2(0h), 10. 6(4h), 9. 8(8h). The IL-2 and IFN-γ production of splenic lymphocytes + lewis cells was (25.9 ± 1.4) pg/ml, (56. 0± 3. 5 ) pg/ml. The IL-2 and IFN-γ production of splenic lymphocytes + lewis/mB7-1-GPI was ( 871.3 ± 10. 4 ) pg/ml, ( 1329. 0 ± 11.9 ) pg/ml. In 25 days, the mean diameter of tumor of Lewis/mB7-1 -GPI was shorter than Lewis( 1.4 ± 0. 21 )cm & ( 2. 5 ± 0. 27 )cm , P ＜ 0. 05 ). Lewis tumor cell-bearing C57BL/6 mice treated with Lewis/mB7-1-GPI vaccine survived much longer than mice treated with Lewis vaccine ( 75.2 ± 2. 0 ) d & (40. 2 ± 2. 0 ) d ( P ＜ 0. 05 ). Conclusion The Lewis tumor vaccine prepared with mB7-1-GPI fusion protein significantly inhibited the tumor growth in Lewis bearing mice. It represented an useful new strategy for attaching immunological factor onto tumor cell surfaces without genetic manipulation.

Objective To study the absorption mechanism of free bone autograft and compare the advantages of the membranous and endocbondral bone autografts in rabbits. Methods Thirty adult male New Zealand white rabbits were randomly divided into two groups of fifteen each. Cranium graft or rib graft was implanted on each side of the snouts of the animal. The onlay grafts were harvested at 12th and 24th week. Then gross observation, volume measurement, histological study and scanning electron microscopical observation were performed.Results Cranium graft underwent less resorption than rib graft and the cortical bone underwent more resorption than cancellous bone. The number and arrangement of collagen fibrils at 24 weeks was more and better than that of 12 weeks. The collagen fibrils of the cranium graft were more numerous and regular than those of the rib graft at 12 weeks, but they were similar at 24 weeks. Although the grafts survived, their volume continued to decrease due to stress. Conclusions Membranous bone implanted on cranium of the rabbit is better than endochondral bone. Remodeling time of a cranium graft is shorter than a rib graft. The graft volume is also relating to stress.

Objective To study the absorption mechanism of free bone autograft and compare the advantages of the membranous and endocbondral bone autografts in rabbits. Methods Thirty adult male New Zealand white rabbits were randomly divided into two groups of fifteen each. Cranium graft or rib graft was implanted on each side of the snouts of the animal. The onlay grafts were harvested at 12th and 24th week. Then gross observation, volume measurement, histological study and scanning electron microscopical observation were performed.Results Cranium graft underwent less resorption than rib graft and the cortical bone underwent more resorption than cancellous bone. The number and arrangement of collagen fibrils at 24 weeks was more and better than that of 12 weeks. The collagen fibrils of the cranium graft were more numerous and regular than those of the rib graft at 12 weeks, but they were similar at 24 weeks. Although the grafts survived, their volume continued to decrease due to stress. Conclusions Membranous bone implanted on cranium of the rabbit is better than endochondral bone. Remodeling time of a cranium graft is shorter than a rib graft. The graft volume is also relating to stress.