Objective To investigate the effect of killing the biofilm persisters by miconazole combined with two compound which can inhibit the drug efflux of C. albicans. Methods Lab reference strains of C. albicans YEM30(CAF2-1) formed the mature biofilm in the 96 well plates, and then treated with miconazole combined with Enniatin B (CDR1 inhibitor) and Milbemycins ot25 (CDRI/CDR2 inhibitor) respectively. After incubated for 48 hours by CFU counting on the YPD plates, the analysis of persisters with SAS8.0 software package for q test. Results Miconazole combined with drug efflux inhibitor can kill more persisters than miconazole alone(P <0.001), and combining with Enniatin B have a better results in eliminating the biofilm persisters than with Milbemycins α25. Conclusion Antifungal drugs combined with drug efflux pump inhibitors can kill more strains which can tolerate very high concentration of antifungal drugs. And searching the potential drug efflux pump inhibitors may be a new way for eliminating the persisters in biofilm, and consequently controlling the chronic recurrent fungal infectious diseases.

OBJECTIVE To probe how to avoid the complication after intravenous infusion for the elders. METHODS The cause of the complication in infusion therapy for the elders with a total of 80 cases in the past three years was analyzed retrospectively. RESULTS Among the complications after intravenous infusion for 80 elders,leakage injury accounted for 47 cases(58.5%), and phlebitis accounted for 21 cases(26.8%),respectively. CONCLUSIONS As the elders′ blood vessel wall is very thin,the intravenous infusion often causes injury,the nurses should pay more attention to operating of intravenous infusion for the elders,observing regularly,and prolonging the time for pressing the pinprick after pulling out the dropper,in order to reduce the coming of injury.

Objective:The research investigated the characters of college students' autonomy and its relation to social adjustment.Methods:650 college students from Xi'an completed The Self-Regulatory Questionnaire and The Chinese College Student Adjustment Scale (CCSAS).Results:① Boys' autonomy level was higher than girls';There were no significant differences of autonomy level in the first three years of college,but the senior year appeared a slight reduction.②There were significant correlations between autonomy and social adjustment.③Students with higher autonomy had significant better social adjustment than lower autonomy students.④ Autonomy had direct effect on social adjustment.Conclusion:There are significant correlations between autonomy and social adjustment.

Objective To analyze the relationship between clinical features of infantile cough after infection and microelements in peripheral blood, and to observe the therapeutic of treating this cough with Milkvetch Root and Radix Glycyrrhizae. Methods 75 infantile patients were randomly recruited into a therapeutic group and a reference group. 39 patients in the therapeutic group were treated with Huangqi Zhusheye and Tablete Glycyrrhiza, while 36 patients in the reference group were treated with Azithromycin. The therapeutic effects were evaluated after the treatment. Meanwhile,microelements in peripheral blood of the 75 patients were assayed and made a contrast to 40 healthy children. Results The contents of Fe, Zn, and Mg in peripheral blood of the 75 patients dropped on different levels, comparing to the healthy children.The therapeutic effects of the therapeutic group was much better than the reference group with significant difference (P＜0.05).Liver and renal functional lesion was unseen in both groups after the treatment. Conclusion Infantile past-infection cough may related with the decrease of Mg, Fe, and Zn. Combined usage of Milkvetch Root and Radix Glycyrrhizae clinically has good therapeutic effects to this disease.

With the occurrence of wireless sensor networks,low-load monitoring has a revolutionary change.Using wireless sensor networks,the whole monitoring system is composed of wireless electrocardiogram(ECG)sensors and wireless pulse wave sensors.Pulse wave signals from the temporal artery and ECG signals from the body synchronously were measured.Pulse Wave Transit Time was calculated.Both the wireless ECG sensor and the wireless pulse wave sensor were small sized and powered by button battery;the precision of time synchronization among sensors was less than 1 ms.The calculated Pulse Wave Transit Time changed slowly with deep breathing.The system iS able to work smoothly for continuous monitoting of Pulse Wave Transit Time in working conditions.

Astemizole is nitrasoted with sodium nitrite in 0.12 mo l/L HAc solution at boiling bath for 25 min. The nitrosation product of astemizo le shows a reduction peak by single-sweep oscillopolarography. The peak potenti al is -0.72V (vs.Ag/AgCl). The first-order derivative peak current is propo rtional to the concentration of astemizole in the range of 4.0×10-7～1.6 ×10-5 mol/L (r=0.9997), with detection limit of 2.0×10-7 mol/L . The proposed method has been applied to the determination of astemizole in pha rmaceutical tablets.

0.05). In RAC group, the Na +,K + ATPase activity of the left kidney decreased compared with that of the right kidney (1.96?0.42 vs 5.33?0.77, P

Objective To detect the expressions of Six1 ,TGF‐βand their common induced VEGF‐C in human laryngeal squa‐mous cell carcinoma ,and to explore their significance in the occurrence ,development ,metastasis and prognosis of laryngeal squa‐mous cell carcinoma .Methods The clinical data and preserved paraffin samples of 96 patients with laryngeal squamous cell carci‐noma confirmed by postoperative pathology were collected .The protein expression of Six1 ,TGF‐βand VEGF‐C was determined by adopting immunohistochemistry and Western blotting .Results The positive expression rates of Six1 ,TGF‐β and VEGF‐C in the laryngel squamous cell carcinoma tissue samples were 84 .4% (81/96) ,89 .6% (86/96)and 91 .7% (88/96)respectively .The positive expression rate of Six1 was significantly higher in the patients with poor differentiation and lymph node metastasis ;the positive ex‐pression rate of TGF‐βwas significantly higher in the patients with lymph node metastasis and had no relationship with the degree of differentiation ;the positive expression rate of VEGF‐C was significantly higher in the patients with poor differentiation and lymph node metastasis ;there was a positive correlation between expression of Six 1 and VEGF‐C in the laryngel squamous cell canc‐er tissue .Conclusion The high expression of Six1 ,TGF‐βand VEGF‐C may promote lymphatic metastasis of laryngeal squamous cell carcinoma .Six1 expression may be one of the important influencing factors of the expression change of VEGF‐C .The combined detection of Six1 ,TGF‐βand VEGF‐C has an important clinical significance for judging the metastasis and prognosis of LSCC .

Objective To study the expression and clinical significance of Survivin and PTEN in cervical carcinoma. Methods The expression of Survivin and PTEN in 60 cases of cervical carcinoma, 15 cases of CIN Ⅰ , 15 cases of CIN Ⅱ, 15 cases of CIN Ⅲ and 15 cases of normal cervical tissues were detected by immunohistochemical staining. Results The expression positive rates of Survivin were 86.67 %, 80.00 %,33.33 %, 20.00 % and 0, respectively, and those of PTEN were 21.67 %, 40.00 %, 80.00 %, 86.67 % and 100.00 % in cervical carcinoma, CIN Ⅲ, CIN Ⅱ, CIN Ⅰ and normal cervical tissues, respectively. The expression positive rates of Survivin were increased along with normal cervical epithelium, CIN Ⅰ, CIN Ⅱ,CIN Ⅲ and invasive carcinoma of cervix;while those of PTEN were inverse order.Compared with the expression positive rates of Survivin and PTEN protein in the CIN Ⅱ group, those in the CIN Ⅲ group had significant differences (P <0.01 and P <0.05, respectively). Expressions of Survivin and PTEN were correlated to the size of focus(P <0.05), the depth of tumor and pelvic lymphnode metastasis (P <0.05), but not to clinical staging, pathological types, pathological grading and age(P >0.05). There was a negative correlation between the expression of PTEN and Survivin in cervical carcinoma. Conclusion The expressions of Survivin and PTEN are correlated with invasion and metastasis of cervical carcinoma, and be related to clinical pathotology.Survivin and PTEN may play a important role in the formation, proliferation, differentuation and metastasis of cervical carcinoma. They may be used as markers of early diagnose, efficacy and prognosis evaluation.

Objective To study the toxic effect of exotoxin A of Pseudomonas aeruginosa on keratocytes.Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A(0.1,1.0,10 mg?L-1),cultivated for 24 h at 37℃,the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase(LDH) was measured by enzyme linked immunosorbent assay(ELISA).Results The LDH contents in different concentrations(0.1,1.0,10 mg?L-1)of exotoxin A groups were higher than that in the group without exotoxin A(P