Objective:To investigate the expression of metastatic suppressor Kangai 1(KAI1) expression in the primary gallbladder carcinoma and its clinical significance.Methods: The clinical stages of 35 primary gallbladder carcinoma patients were determined according to the TNM criteria of International Union Against Cancer(UICC).Histopathologic changes and the expressions of KAI1 were detected by H-E and immunohistochemical methods,respectively.All the patients were followed up for 3 years.Results: It was found that 1 patient had carcinoma in situ(0 stage),13 had early carcinoma(Ⅰ-Ⅱ stage),and 21 had intermediate or advanced carcinoma(Ⅲ-Ⅳ stage).Histopathologic grading showed that 14 patients had well-differentiated carcinomas,10 had moderately differentiated ones,and 11 had poorly differentiated ones.Immunohistochemical results revealed that 60.00%(21/35) of the patients were positive of KAI1,with the positive rate being 92.31%(12/13) in early carcinoma(Ⅰ-Ⅱ stage) and 42.86%(9/21) in intermediate and advanced carcinoma(Ⅲ-Ⅳ stage),and the former was significantly higher than the latter(P_(exact)=0.00 476).The positive rate of KAI1 protein was negatively correlated with the clinical staging of patients(P_(trend)=0.004) and positively correlated with histopathologic grading(P_(trend)=0.001).The positive rate of KAI1 protein in well differentiated carcinoma was 92.86%(13/14),in moderately differentiated carcinoma was 60.00%(6/10),and in poorly differentiated carcinoma was 18.18%(2/11)(P_(exact)=0.000 475 between the 3 groups).The positive rate of KAI1 protein was 89.47%(17/19) in 19 survival patients and 25.00%(4/16) in 16 death cases.Obvious significance of the expression of KAI1 protein was found between the 2 groups(P=0.001).Conclusion: The positive rate of KAI1 is negatively correlated with the clinical staging but positively correlated with the histopathologic grading in patients with primary gallbladder carcinoma.The high expression of KAI1 genes may indicate a better prognosis of gallbladder carcinoma.

Objective:To examine the surface of mineral trioxide aggr eg ate (MTA) when treated in different conditions in vitro. Method: Root blocks were prepared. MTA and other three dental materials (Dycal, Dyr act and amalgam) were respectively filled into the root canals of prepared root blocks. The roots with the materials were maintained in distilled water or simul ated body fluid (SBF) at 37 ℃ for 5 days. Scanning electron microscopy(SEM) and X-ray energy dispersive spectroscopy(EDS) were employed to investigate the mor phological changes and chemical components. Results:There was pr ecipitate of simple crystal units or crystalline structure in the MTA specimens. EDS showed one or two peaks corresponding to calcium or calcium and phosphorus. Conclusions:Apatite may produce and grew by MTA on its surface.

Objective To study the effect of powder of Chinese Angelica and Peony (DSS) on hippocampus proteome of senescence accelerated mice (SAM). Methods Thirty female mice aged 12 months were used in our study, including 10 SAM-resistance/1 (SAMR1) and 20 SAM-prone/8 (SAMP8). The difference of hippocampus proteome among SAMP8, SAMR1, SAMP8 treated with DSS intragastrically at dose of 3.2 g/kg once a day for 30 d (SAMP8+DSS), was analyzed by two dimensional polyacryalmide gel electrophoresis (2DE). Results Compared with SAMR1, the expressions of 13 proteins in hippocampus of SAMP8 were up-regulated and that of 9 proteins were down-regulated significantly. Compared with SAMP8, the expressions of 21 proteins in hippocampus of SAMP8+DSS were up-regulated and that of 14 proteins were down-regulated significantly. Using MALDI-TOF-MS, the proteins with significant changes in expressions were identified by peptide fingerprinting map and the results were searched in MASCOT database, and sorted into 5 function groups including mitochondria-associated proteins, energy metabolism proteins, synaptic plasticity formation proteins, cytoskeletal proteins and others proteins. Conclusion The effects of DSS on hippocampus proteome of senescence accelerated mice were probably exerted via multi-target and multi-pathway mechanism.

Objective To analyze and compare the anti-HCV reactivity,HCV nucleic acid detection results and HCV recom binantion immunoblot assay(RIBA) confirmatory test results in blood donors.Methods The blood samples collected from the volunteer blood donors from October 2013 to March 2015 were performed the HCV screening by using the domestic ELISA reagents from two different manufacturers and an imported nucleic acid detection reagent and matching detection system.The samples of anti-HCV reactivity or/and NAT detection positive were performed RIBA.Then the results of reactivity detected by two kinds of ELISA reagents,nucleic acid detection reagent and RIBA confirmatory test results were analyzed and compared.Results A total of 133959 samples of volunteer blood donors were detected,in which 113 380 samples covered the nucleic acid detection results,the reactivity samples proportion of anti-HCV detection was 0.19 ％ (252/133959),27 cases were positive in NAT detection with the positive detection ratio of 0.02 ％ (27/113 380);the proportion of HCV reactive samples confirmed by RIBA was 19.8 ％ (50/252),the negative proportion was 54.8％ (138/252),and the uncertain proportion was 25.4％ (64/252);27 samples of nucleic acid detection positive were double reagent reactivity in ELISA detection and positive in confirmatory test.The difference among the results of two ELISA reagents,RIBA confirmatory test results and nucleic acid detection results had statistical significance(P＜0.05).Conclusion The detection strategy selecting twice ELISA+1 kind of nucleic acid detection is more secure.Aiming at higher proportion of false positive samples,the follow up system of blood donors should be established for maximizing the retention of blood donors.

Through the excellent experiment of cardiovascular system in pathology, the prac-tice of the teaching reform was carried out such as combining flow model with real specimens, digital sections with light microscope slides, inserting the use of special staining experiments in combination with digital medical image analysis and the simulation of cardiovascular clinical pathological case dis-cussions. Linking theory with experiment teaching and experiment teaching with clinical practice was focused on, which not only stimulated students' interest in learning and their exploring thinking and hands-on ability, but also promoted them to obtain good learning effect, thus improving the teaching quality.

0.05). In RAC group, the Na +,K + ATPase activity of the left kidney decreased compared with that of the right kidney (1.96?0.42 vs 5.33?0.77, P

Department of Regenerative Medicine of Tongji University School of Medicine intro-duced the standardized question library construction-based separation of teaching from testing system into integrated life science course. By establishing the question library, professional teachers in the assessment center are responsible for making and correcting test papers of final exam for students. In addition to the separation of teaching and testing, regular quiz during the semester is also involved in the final grades of students. The results show that high-quality question library effectively promotes implementation of separation of teaching from testing. The question library construction is a dynamic and long-term task that requires real-time updates along with knowledge updates. This preliminary practice of separation of teaching from testing system in the integrated life sciences curriculum has proved to be useful for improving teaching style and the style of study significantly.

Objective To screen monoclonal antibodies (mAbs) for early diagnosis of invisive Aspergillus. Methods Monoclonal antibodies against different antigens of Aspergillus fumigatus were produced. The two pairs of combinations of monoclonal antibodies were selected accoring the distinct epitopes and double-antibody sandwich ELISA based on mAbs above were established. The sensitivity and specificity of the methods were analyzed by detecting culture supernatants of clinical isolates and environmental isolatesof Aspergillus. spp, Penicillium Marneffei, Candidas, and serum from animal models and patients. The epitopes recognized by mAbs were identified by immunobotting. Results A total of 32 hybridoma cell lines that stably produced MAbs were obtained. Two double- antibody sandwich ELISAs were established. One method was specific for 19 clinical isolates and environmental isolates of Aspergillus. spp, whereas the other one was specific for the clinical and environmental isolates of Aspergillus fumigatus without cross-reation with other Aspergillus. spp. For the same kind of medium of Aspergillus fumigatus, the sensitivity of the first method was 10 fold higher than the second method. Conclusions The specific mAbs for early diagnosis of invisive Aspergillus were obtained. Antigen recognized by the specific mAbs was mannoprotein with molecular weights of approximately 25 000-75 000. This antigen was potential early diagnostic marker for invasive Aspergillus.

Objective To investigate whether the proteasomes inhibitor MG262 exerts its anticancer function by inducing apoptosis in human ovarian cancer cells,and whether the extracellular signal regulated kinase (ERK) signaling pathway is involved in the regulation of apoptosis induction.Method Human ovarian cancer cell line SKOV3 was incubated with different concentrations of MG262 for 24 and 48 hours.Cell viability was evaluated with 3-(4,5-dimethyhhiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at different time points of culturing.Flow cytometry was used to detect cell apoptosis rate.The expression of vascular endothelial growth factor (VEGF) was evaluated with western blot and enzyme-linked immtmosorbent assay (ELISA).Western blot was used to detect the expression of phosphorylated ERK(pERK) .Results The viability of SKOV3 cells was decreased by MG262 in a concentration-dependent fashion(P＜0.05).After 24 h incubation with MG262 at 1,10,20,40,60 and 80 nmol/L,the viability rates of SKOV3 were (94.6±3.1)%,(92.7±3.7)%,(89.5±7.7)%,(84.2±5.1)%,(82.0±7.4)%and(76.8±11.0) % respectively,and after 48 h incubation,those figures were further decreased to (91.3±10.1)%,(86.8±4.5)%,(74.6±4.2)%,(56.8±2.1)%,(49.3±4.5)% and (37.4±5.4) %,respectively(P＜0.05).Apoptosis rate of SKOV3 cells induced by MG262,PD98059 or their combination was (30.7±4.3)%,(26.8±8.6)% and (50.3±10.6)%,respectively,which were significantly different compared with controls (P＜0.05).In contrast to SKOV3 cells,apoptosis rate of 293T ceils induced by MG262,PD98059 or their combination was (14.5±5.3) %,(16.2±7.5) % and (10.8±7.3)%,respectively,which were not significantly different compared with controls (P＞0.05).pERK expression decreased gradually in a time-dependent manner. And wild-type p53 expression was not significantly different.There was no significant difference between experimental and control 293T cells(P＜0.05).In addition,MG262 down-regulated VEGF secretion and expression in SKOV3 ceils (P＜0.05).Conclusions Proteasome inhibitors can induce apoptosis and inhibit cell proliferation and angiogenesis through ERK signal pathway in SKOV3 cells.

Objective:To explore the clinical value of stereotactic wire-localized biopsy(SWLB)in digital mammography in the detection of microcalcification of the breast.Methods:A total of 45 patients with nonpalpable breast lesions which were positive for microcalcification by mammography but could not be detected clinically underwent SWLB.Their mammography fndings were analyzed in detail with pathology.Results:Among the 45 cases,13 cases(28.9%)had malignant lesions including ductal carcinoma in situ in 3 cases (20.1%),ductal carcinoma in situ with microinvasion in 4 cases(30.8%),invasive ductal carcinoma in 5 cases (38.5%)and intraductal papillary carcinoma in 1 case(7.7%).Thirty-two cases(71.1%)had benign lesions,2 cases(6.3%)of which were severe atypical hyperplasia.Conclusion:SWLB can accurately guide the surgical excision of nonpalpable breast microcalcification lesions and diagnose microcalcifications exactly,which is helpful for increasing the detection rate of eady-stage breast cancer.