Objective:To study the expression of matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of metalloproteinase-1(TIMP-1) protein in the implantation window endometrium phase in ovulation normal women and the role of MMP-9/TIMP-1 on embryo implantation.Methods:SEM was used to determine “implantation window” of the mid-secretive phase endometrium in ovulation-normal women.Immunohistochemical analysis was applied to measure the expression of MMP-9/TIMP-1 protein in the implantation window in 15 cases,and MMP-9/TIMP-1 expression in endometrium during different phases were compared.Results:MMP-9/TIMP-1 expression in endometrium was observed in luminal,glandular epithelium and stromal cells in different phases.The expression was lower in proliferative phase,and got to the summit in the implantation window and late secretive phase and it expressed significantly higher in the implantation window phase(P

Objective: To study the relation between the expression of matrix metalloproteinase-9(MMP-9) / tissue inhibitor of metalloproteinase-1(TIMP-1) protein in the implantation window endometrium phase and serum estradiol and progesterone.Methods: 31 women with regular ovulation were included to obtain endometrium samples in implantation window phase,and at the same time serum were abstracted from vein for E2 and P testing.Immunohistochemical analysis was applied to measure the expression of MMP-9/TIMP-1 protein in the implantation window.Analysis the correlationship between MMP-9/TIMP-1 and E2 and P.Results: The light dense of the expression of MMP-9 / TIMP-1 protein in the endometrium of implantation window were 0.163?0.025,0.143?0.015 respectively,and the corresponding serum E2,P were 182.153?45.774pg/ml,22.997?6.697ng/ml respectively.There was negative correlationship between MMP-9 and P(P

0.05).There was the most and the least oocytes in group 1(P/E2200),respectively.No patient in group 5 became pregnant finally,while those in group 3(P/E2100~150) got higher pregnancy rates and single embryo implantation rates than other four groups(P

Objective To study the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) proteins in the decidua and villi of the early pregnancy. Methods Immunohistochemical staining was applied to detect the expression of MMP-9 and TIMP-1 proteins in the decidua and villi of 45 the early pregnant women and in the mid-secretory phase endometrium of 30 normal women. Results The OD of MMP-9 and TIMP-1 proteins expression in the decidua and villi was 0 168?0 055 and 0 155?0 046 respectively, in the mid-secretory phase endometrium were 0 133?0 0210 and 0 113?0 0251 respectively, and the expressions of MMP-9 and TIMP-1 in the early pregnancy increased significantly when compared with the mid-secretory phase endometrium(P

Objective To explore the changes of plasma corticotrophin releasing factor (CRF) levels in the young rats with hypoxic-ischemic brain damage.Methods Two hundred and forty young rats were randomly divided into three groups:hypoxic-ischemic brain damage group ( model group,n =80),sham-operated group ( n =80),and normal control group ( n =80).The plasma CRF levels of rats in three groups were detected at 1 h,3 h,6 h,12 h,l d,3 d,5 d and 18 d after hypoxia-ischemia,per ten rats for each time point.Plasma CRF levels were measured by radioimmunoassay.Results Plasma CRF levels of model group,shamoperated group and normal control group showed no significant difference in the young rats after 1 h,3 h,6 h,12 h of hypoxia-ischemia ( P ＞ 0.05 ).But plasma CRF levels in the model group were respectively significantly lower than those of sham-operated group and normal control group after 1 d and 3 d of hypoxia-ischemia ( P ＜0.001 ),and then recovered to the control group levels after 5 d and 18 d of hypoxia-ischemia ( P ＞0.05 ).Conclusion Hypoxia-ischemia affects plasma CRF levels in the young rats,which is related with the duration after hypoxia-ischemia.

In order to meet the needs of contemporary society for medicine and cultivate high-quality compound medical talents, Chongqing Medical University has carried out the "organ system-centered" urogenital system integration curriculum reform. In the practice of integrated curriculum teaching, students have deepened their systematic understanding of medical knowledge, enhanced their enthusiasm and initiative in classroom learning, cultivated students' logical thinking ability, and improved students' innovative scientific research ability, by reorganizing the curriculum system, rewriting textbooks, improving the teaching environment, forming a teaching team, and reforming teaching methods, which laids the foundation for the further improvement of the medical curriculum.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.