Objective To evaluate the impact of low dietary glycemic index on the risk of coronaryheart disease (CHD).Methods We searched the Cochrane Database of Systematic Reviews,JBI Database of Systematic Reviews,MEDLINE,EMbase,CBMdisk,and CNKI to collect cohort studies from 2000 to 2011.The quality of cohort studies was critically appraised and data were extracted by 4 reviewers independently.Meta-analysis were conducted for the eligible cohort studies using RevMan 5.0.Results Five cohort studies were included,and 128 911 samples were researched.The result of meta analysis indicated that low dietary glycemic index was a protective factor of CHD,RR value was 1.25,95％ CI was (1.13,2.15).Further more,the result was only effective to female,RR value was 1.35,95％ CI was (1.16,1.57),but not to male,RR value was 0.96,95％CI was (0.76,1.31).Conclusions Low dietary glycemic index can decrease the risk of CHD,but it is still to be proved whether it is suitable to male.

As the main body of providing medical and health services, public hospitals always bear the important responsibility of maintaining people′s life and health.In the period of normal epidemic prevention and control, public hospitals are also facing a series of new situations, including changes in social environment, demands for reform and development and transformation of target functions. It is urgent to solve many problems, such as inadequate capacity building, continuous heavy operational pressure, impact on traditional modes, and constant adjustment of positioning and mode.Therefore, both the state and the government should adhere to the public welfare as the fundamental standpoint, accelerate the implementation of scientific planning and balanced distribution of medical resources, through the fundamental adjustment of the system and mechanism, and actively help public hospitals to choose the appropriate development mode, as soon as possible to relieve pressure rescue; Public hospitals should actively make use of the extensive application of Internet and other technologies and the huge potential contained in scientific and technological innovation to achieve high-quality development goals of improving quality and efficiency, and provide more powerful support for the overall national strategy.

OBJECTIVE: To explore the origin and mechanism of small supernumerary marker chromosomes (sSMC) in three fetuses. METHODS: The three fetuses were predicted to have carried chromosomal abnormalities by non-invasive prenatal testing (NIPT). G-banding chromosomal karyotyping analysis were carried out on amniotic fluid samples of the fetuses and peripheral blood samples from their parents. Single nucleotide polymorphism array (SNP-array) was used to determine the origin, size and genetic effect of sSMCs. RESULTS: In fetus 1, SNP array has detected two microduplications respectively at 4p16.3p15.2 (24.7 Mb) and 18p11.32q11.2 (20.5 Mb) which, as verified by fluorescence in situ hybridization (FISH), have derived from a balanced 46,XY,t(4;18)(p15.2q11.2) translocation carried by its father. Fetus 2 has carried a de novo microduplication of 15q11.2-q13.3 (9.7 Mb). The sequence of SMC in fetus 3 has derived from 21q11.2-q21.1 (8.3 Mb), which was inherited from its mother. CONCLUSION Both NIPT and SNP-array are highly accurate for the detection of sSMC. SNP-array can delineate the origin and size of abnormal chromosomes, which in turn can help with clarification of sSMC-related genotype-phenotype correlation and facilitate prenatal diagnosis and genetic counseling for the family.
Chromosome Duplication/genetics* ; Female ; Fetus ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Polymorphism, Single Nucleotide ; Pregnancy ; Prenatal Diagnosis ; Translocation, Genetic/genetics*

Objective:To investigate the effects of programmed death-ligand 1 (PD-L1) expression on the biological behaviors of gastric cancer cell line MKN45.Methods:The PD-L1 gene of gastric cancer cell line MKN45 was silenced by RNA interference technique. MKN45 cells were divided into blank control group, si-NC group (transfected with siRNA-NC) and si-PD-L1 group (transfected with siRNA-PD-L1). Quantitative real-time PCR was used to detect the mRNA expressions of PD-L1 and epithelial-mesenchymal transformation (EMT)-related proteins E-cadherin, Vimentin and Snail in MKN45 cells, and Western blotting was used to detect the expression levels of PD-L1 protein in MKN45 cells of each group. Transwell migration test, Transwell invasion test and MTT test were used to detect the migration, invasion and adhesion abilities of MKN45 cells.Results:The relative expression levels of PD-L1 mRNA in the blank control group, si-NC group and si-PD-L1 group were 1.002±0.092, 1.005±0.121 and 0.237±0.017, respectively, with a statistically significant difference ( F=75.61, P<0.001). The protein expression levels of PD-L1 in the three groups were 0.944±0.028, 1.008±0.088 and 0.269±0.015, respectively, with a statistically significant difference ( F=172.99, P<0.001). The mRNA and protein expression levels of PD-L1 in the si-PD-L1 group were lower than those in the other two groups (all P<0.001), but there were no statistically significant differences between the blank control group and si-NC group (all P>0.05). The cell migration rates of the blank control group, si-NC group and si-PD-L1 group were (1.000±0.020)%, (1.012±0.084)% and (0.488±0.050)%, respectively, with a statistically significant difference ( F=80.73, P<0.001). The cell invasion rates of the three groups were (0.929±0.087)%, (0.924±0.208)% and (0.300±0.100)%, respectively, with a statistically significant difference ( F=19.37, P<0.001), and the cell adhesion rates of the three groups were (100.000±5.407)%, (99.280±4.845)% and (59.723±2.674)%, respectively, with a statistically significant difference ( F=79.87, P<0.001). Compared with the blank control group and si-NC group, the migration, invasion and adhesion abilities of MKN45 cells in the si-PD-L1 group decreased significantly (all P<0.001). The expression levels of E-cadherin mRNA of the three groups were 1.000±0.023, 0.981±0.051, 3.618±0.201, the expression levels of Vimentin mRNA were 1.000±0.043, 1.108±0.150, 0.328±0.011, the expression levels of Snail mRNA were 1.061±0.103, 1.090±0.110, 0.304±0.043, respectively, with statistically significant differences ( F=477.17, P<0.001; F=65.97, P<0.001; F=72.70, P<0.001). Compared with the blank control group and si-NC group, the mRNA expression levels of Vimentin and Snail of MKN45 cells in the si-PD-L1 group decreased, while the expression level of E-cadherin mRNA increased, with statistically significant differences (all P<0.001). Conclusion:Silencing the expression of PD-L1 can reduce the migration, invasion and adhesion abilities of MKN45 cells, and the mechanism may be related to the effect of PD-L1 on the EMT pathway of gastric cancer.

The miR-34 family plays an important role in gastric cancer, and the inactivation or reduced expression of the miR-34 family is detected in gastric cancer cell lines and gastric cancer tissues compared with normal gastric mucosa tissues, indicating it is associated with the occurrence and development of gastric cancer. Studies have shown that miR-34 plays a key role in inhibiting gastric cancer progression by regulating IGF2BP3, survivin, Bcl-2 and epithelial-mesenchymal transition-related pathway, indicating that miR-34 is an important target for gastric cancer treatment. In terms of clinical treatment, miR-34 has not only been proved to have radiochemotherapy sensitization, but also achieved good curative effect in tumor clinical trials. With the emergence of miR-34 vectors targeting gastric cancer, it is possible to use it for gastric cancer treatment. Deep understanding of the molecular basis and clinical efficacy of miR-34 for gastric cancer treatment can help to evaluate the potential of the miR-34 family as a new therapeutic target for gastric cancer.

Humans ; Norepinephrine ; Bacterial Infections/drug therapy* ; Immunity, Innate

Percutaneous coronary intervention (PCI) has become an important means of revascularization for patients with coronary heart disease (CHD) . Exercise therapy is the core content of cardiac rehabilitation for patients after PCI. Baduanjin, one of the traditional exercise methods of traditional Chinese medicine that spreads widely and enjoys obvious fitness effects, shows a positive impact on the rehabilitation of patients after PCI. This article reviews the effects of Baduanjin on the cardiopulmonary function, psychological status, quality of life, physical and chemical indicators, and adverse cardiovascular events in patients after PCI from different perspectives, in order to provide a reference for the practice and research of Baduanjin in the rehabilitation of patients after PCI.

Objective To explore application of feedforward control nursing intervention in anesthesia recovery management of patients undergoing robot-assisted thoracoscopic radical resection for lung cancer.Methods Sixty-four patients underwent robot-assisted thoracoscopic radical resection for lung cancer at People's Hospital of Liaocheng City in Shandong Province from December 2021 to May 2022 were selected as research objects.They were randomly divided into control group and experimental group,with 32 cases in each group.Control group received routine nursing measures,while experimental group received nursing intervention under guidance of feedforward control on basis of control group.Pass rate of nurses relevant theoretical knowledge,incidence of perioperative complications,nursing satisfaction,nursing quality score,recovery room stay time,self-rating anxiety scale(SAS)and self-rating depression scale(SDS)scores before operation and when leaving recovery room were compared between two groups.Results Pass rate of nurses relevant theoretical knowledge in experimental group was significantly higher than that in control group(P<0.05).Total incidence rate of pressure injury,incidence rate of fluid circuit problems,agitation and hypothermia in experimental group were significantly lower than those in control group(P<0.05).Nursing effect,nursing professionalism,nursing operation and nursing attitude scores of experimental group were significantly higher than those of control group,and recovery room stay time of experimental group was significantly shorter than that of control group(P<0.05).When leaving recovery room,SAS and SDS scores of experimental group were significantly lower than those of preoperative in this group and control group(P<0.05).Conclusion Application of feedforward control nursing intervention in robot-assisted thoracoscopic lung cancer patient anesthesia recovery management can effectively reduce incidence of perioperative complications,shorten recovery time,improve nursing satisfaction and nursing quality scores,and improve patients'early postoperative anxiety and depression status,which is worthy of clinical application and promotion.

Objective:To investigate the effect of Mediterranean diet on blood glucose control and cardiovascular risk factors in patients with type 2 diabetes.Methods:As to December 2021, the PubMed, Cochrance Central Register of Controlled Trials and Cochrance Database, Cochranc Library, Embase, China National Knowledge Infrastructure and Wanfang Medical Network system were searched for clinical randomized controlled trials(RCTs) of Mediterranean diet in patients with type 2 diabetes to conduct Meta-analysis The main observation index were cardiovascular risk factors, and the mean difference and its 95% confidence interval were used to estimate the effect size.Results:There were six RCTs, and 1181 patients met the inclusion criteria and entered the Meta-analysis. Compared with the control group, the intervention group can significantly reduce the level of systolic blood pressure ( MD=-1.20, 95% CI-2.21 to -0.19) and diastolic blood pressure ( MD=-4.17, 95% CI-7.12 to -1.22) in patients with type 2 diabetes mellitus, but there were no significant difference in the level of TC ( MD=2.92, 95% CI-0.84 to-6.67), HDL ( MD=2.33, 95% CI-0.27 to -4.92) and LDL ( MD=-2.34, 95% CI-5.67 to -0.99) between the two groups (all P>0.05). Conclusions:The meta-analysis provided evidence the Mediterranean diet showed the beneficial improvements in blood pressure glycemic control, but the effect of Mediterranean diet on lipid profile was not significant, which needed further verification.

Objective:To understand the epidemiological characteristics of human adenovirus(HAdv)in Xuzhou area.Methods:A total of 7 257 swab samples from influenza-like illness children in Xuzhou children’s Hospital were collected during 2013-2019. Real-time PCR was used to detect adenovirus, and then the positive specimens were subjected to amplification for the hexon gene by polymerase chain reaction (PCR) for genotypic analysis. Adenovirus 55 was cultured by Hep-2 cells and analyzed by whole gene sequencing.Results:A total of 227 strains of adenoviruses were detected out of 7 257 samples, and the detection rate was 3.13%. Infections were mainly found in children aged 3 to 6, with a peak of detection in May and June. Typing data showed that the infection was mainly adenovirus type 3 and type 7, and the dominant type of adenovirus in different years alternated between type 3 and type 7. Complete gene sequencing showed only a few point mutations in genes of adenovirus type 55 we isolated, and no other gene mutation and recombination were found.Conclusions:The main types of adenovirus were types 3 and 7 among children with inluenza-like illness in Xuzhou from 2013 to 2019, and the infected population was mainly preschool children, and the detection peak was in spring and summer.