Objective: To evaluate the quality of life,peripheral blood and immune function of patients with malignant tumors treated by PSP.Methods: We searched MEDLINE,Pubmed and other databases by computer.We assessed the identified studies in order to include high quality studies.Results:A total of 7 into the study,701 cases of patients.Meta analysis showed that polysacchariopeptide treatment for patients with certain malignant tumors after chemotherapy might improve the quality of life.Polysacchariopeptide group combined with chemotherapy drugs might improve the white blood cells,hemoglobin,platelet compared to chemotherapy drugs or batyl alcohol drugs combined with chemotherapy.Polysacchariopeptide drug combined with chemotherapy may increase the CD_3~+ ,CD_4~+ ,CD_8~+ and CD_4~+/CD_8~+ ratio,and the NK cells was no significant difference.Conclusion:PSP combined with chemotherapy to treat malignant tumors has a certain effect.However,most of trials included in the review may be of low quality,therefore,it may be neccessity to conduct multi-center randomized-controlled trials of high quality.

Objective Tumor suppressor gene p53 can inhibit tumor cell growth, arrest cell cycle, and promote apoptosis.Howev-er, the effects of p53 on the pathogenesis of breast cancer have not been fully elucidated.The aim of this study was to explore the expression of p53 protein and the correlation with clinical pathologic features in breast cancer.Furthermore, the regulatory effects of 5-aza-2′-deoxycyti-dine on p53 in breast cancer cell line were also studied. Methods The expression of p53 protein in 80 cases of breast cancer and normal and adjacent tissue were determined by the immunohistochemical staining .The expressions of p53 mRNA and p53 protein in breast cancer cell line were determined by RT-PCR and Western blotting. Results The positive rate of p53 in breast cancer (41.25%) was higher than that in the normal and adjacent tissue (22.5%) (P<0.01).The expression of p53 was not significantly correlated with age, grade, stage and lymph node metastasis (P>0.05).The low expression of p53 both in mRNA and in protein levels were found in breast cancer cell line of MCF-7.The expres-sion of p53 increased after 5-aza-2′-deoxycytidine administration . Conclusion p53 is highly expressed in breast cancer , which may play an im-portant role in the development and progression of breast cancer. 5-aza-2′-deoxycytidine, up-regulating the p53 expression in breast cancer cell line, which provides the evidents for the development of therapeutic drugs for the patients with low expression of p53 breast cancer.

Objective: To explore the effect and mechanism of Sacubitril/Valsartan on myocardial remodeling and cardiac function in rats with myocardial infarction. Methods: The acute myocardial infarction (AMI) rat model was established by ligating anterior descending branch of coronary artery for one week.A total of 60 adult male rats in SPF grade with AMI were randomized into the Sacubitril/Valsartan group and the model group, who were gavaged with Sacubitril/Valsartan (68 mg/kg, once daily, n=30) versus with normal saline once daily(n=30) for 4 weeks.Twenty-four hours after the last treatment, the left ventricular cardiac function was examined by echocardiography, and pathological changes of the left ventricle were observed under light microscope.The degree of myocardial fibrosis was quantitatively analyzed by picric acid-sirius scarlet staining.Myocardial cells and fibroblasts from rat pups of the same species were prepared in vitro and were divided into the control group, AngⅡ group, LBQ657 group, valsartan group and LCZ696 group.³[H]-leucine incorporation and ³[H]-proline incorporation were used to detect the myocardial hypertrophy and fibrosis. Results: There was no significant difference in left ventricular function between the the model group and the Sacubitril/Valsartan group before medication (P>0.05). Four weeks after administration of the medications, end-diastolic diameter of left ventricle and end-systolic volume of left ventricle were lower [(9.73±0.26) mm vs.(10.52±0.21) mm, P<0.05; (0.19±0.03) ml vs.( 0.31±0.02) ml, P<0.01], and the left ventricular ejection fraction was higher [(60.17±2.18)% vs.(47.16±5.14)%, P<0.01] in the Sacubitril/Valsartan group than in the model group.The degree of myocardial cell injury in the infarct area was lower, and the area of myocardial fibrosis in the non-infarct zone and peripheral infarcted zone were less in the Sacubitril/Valsartan group than in the model group [(4.0±0.1)% vs. (6.1±0.8)%, P<0.001; (15.7±0.8)% vs. (23.8±1.2)%, P<0.001].³[H]-proline incorporation in cardiac fibroblasts was lower in the Valsartan group than in the Ang Ⅱ group [(152.77±8.46) CPM vs.(221.87±13.41) CPM, P<0.01].³[H]-leucine incorporation in myocardial cells was lower in the Valsartan group than in the Ang Ⅱ group [(113.47±2.33) CPM vs.(127.65±2.38) CPM, P<0.01].³[H]-leucine incorporation in myocardial cells was lower in LBQ657 group than in the Ang Ⅱ group [(119.78±2.98) CPM vs.(127.65±2.38) CPM, P<0.05], and the combined application of valsartan and LBQ657 can further reduce myocardial hypertrophy and fibrosis (P<0.05). Conclusions Sacubitril/Valsartan can effectively alleviate myocardial remodeling and cardiac dysfunction after myocardial infarction, and the mechanism may be related to reducing AngⅡ-induced myocardial hypertrophy and myocardial fibrosis.

Objective:To describe the health-promoting lifestyle of caregivers of children with neurodevelopmental disorders and analyze its influencing factors.Methods:A total of 352 caregivers of children with neurodevelopmental disorders in a hospital in Shenzhen were investigated by questionnaire.The main caregivers were investigated with health-promotion lifestyle scale Ⅱ.Generalized linear model was used to analyze the influencing factors of the health promoting lifestyle score of the caregivers by SPSS 20.0 and R 3.6.0 softwares.Results:The total score of health promotion lifestyle for caregivers of children with neurodevelopmental disorders was (127.66±17.87). The standardized score was (62.05±8.96), with the highest standardized score being (69.62±11.04) for the nutrition dimension and the lowest score being (51.67±11.45) for the physical activity dimension.The difference between the groups was statistically significant ( F=62.780, P<0.01). The results of linear regression analysis showed that the education level ( "junior college" vs "primary and junior high school" : β=14.524, t=3.054, P=0.002; "undergraduate and above" vs "primary and junior high school" : β=18.561, t=3.936, P<0.001), care time ( ">3 years" vs " <1 year" : β=-7.156, t=-3.687, P=0.003), and family income ( "10 000-20 000 yuan/ month" vs "<5 000 yuan/month" : β=14.351, t=3.050, P=0.002) were the influencing factors of health promotion lifestyle of caregivers. Conclusion:The life style of caregivers should be paid attention to.It is necessary to help them improve their health-promoting lifestyle through multiform health promotion methods.

Objective:To explore the effect of collaborative nursing based on Snyder's hope theory in patients with decompensated hepatitis B cirrhosis.Methods:From January 2019 to December 2021, 100 patients with decompensated hepatitis B cirrhosis who were admitted to the Affiliated Hospital of Shaoxing University (Shaoxing Municipal Hospital) were selected as the research object. According to the block randomization, the patients were divided into the control group and the observation group, 50 cases in each group. The patients in the control group were given routine nursing. On the basis of the control group, the patients in the observation group were given collaborative nursing based on Snyder's hope theory. Before and after the intervention, the Chronic Liver Disease Questionnaire (CLDQ) , the Herth Hope Index (HHI) and the Chinese version of the Family Adaptability and Cohesion Evaluation Scale (FACESⅡ-CV) were used to evaluate the quality of life, hope and family function of the two groups of patients.Results:Before nursing, there were no statistical differences in CLDQ, HHI and FACESⅡ-CV scores between the two groups ( P>0.05) . After nursing, the CLDQ, HHI, and FACESⅡ-CV scores of the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Collaborative nursing based on Snyder's hope theory can improve the quality of life, hope and family function of patients with decompensated hepatitis B cirrhosis, which is worthy of clinical application.

Objective Based on latent growth mixture model(LGMM)and China Health and Retirement Longitudinal Survey(CHARLS)Phase 3 tracking data,to investigate the development trajectory and influencing factors of the cognitive level of the elderly.Methods The data were from the data of CHARLS in 2013,2015,and 2018,including a total of 4662 elderly people aged 60 and above as research samples.The cognitive level of the elderly was assessed using the MMSE scale.Using the Mplus tool to construct an LGMM model suitable for cohort data to explore the trajectories of cognitive level changes in different elderly groups,examining time-constant factors(gender,age,education level,marital status,smoking,drinking)and time-varying factors(daily life activity ability,sleep duration,subjective life satisfaction)on the cognitive level of the elderly.Results According to the relevant indicators,the development trend of the cognitive level of the elderly can be divided into three types,namely the C1 slow decline group(35.2%),the C2 rapid decline group(20.6%)and the C3 stable group(44.2%).The cognitive level of people showed an overall downward trend with the passage of time.The cognitive level development trajectories of the three groups had significant time effects.The cognitive level of the C2 rapid decline group declined rapidly with time.The cognitive level of and the C1 slow decline group decreased slowly over time,and the cognitive level of the C3 stable group did not change significantly and was in a steady state.Conclusion The developmental trajectory of the cognitive level of the elderly has significant group heterogeneity and individual differences,and there are different types of development trends.