ABSTRACTOBJECTIVE To observe the effect of active components from Lycii cortex on the proliferation of high glucose?in-duced glomerular mesangial cells GMCs and the secretion of extracellular matrix ECM.To investigate the protective mechanism of diabetic nephropathy.METHODS GMCs were cultured and divided into high glucose groupfinal glucose con-centration of 30 mmol/Lblank groupfinal glucose concentration of 5.5 mmol/Lbetaine and kaempferol groups of 5 dif-ferent concentrations of 0.010.1110100 μmol/L.The proliferation of high glucose?induced GMCs were observed by MTT method and secretion of ECM was detected by ELISA assay.RESULTS Different doses of betaine and kaempferol inhib-ited the proliferation of GMCsand decreased the content of ECMcompared with high glucose group P < 0.05 ~ 0.01. CONCLUSION Betaine and kaempferol can protect the high glucose?induced GMCs through inhibiting the proliferation of GMCs and decreasing the content of ECM.