In order to investigate some requisite conditions for the formation of mycelial phase of Pityrosporum orbiculare (P. orb) and understand indirectly the pathogenesis of tinea versicolor, we used a semisolid medium containing cholesterol 1. 0g/dl and agar 0.8g/dl, and obtained the mycelial phase of P. orb. Then we studied the relationship between the formation of the mycelial phase and the concentration of cholesterol or agar in the medium. The results showed that when P. orb were cultured on the medium, the hyphae began to grow on the 4-6th day, increased gradually, and peaked on the 10-12th day. The hyphae were mostly blastohyphae, 8-16?m in length and in the shape of racket,and distributed in clustes. It was found that 0. 25g/dl was the minimum concentration of cholesterol to induce the mycelial phase in vitro. Within the range of 0. 25-1.0g/dl the higher the concentration was, the more the hyphae were. When the concentration exceeded 1.0g/dl, the hyphae did not increase markedly. Relative high moisture (0.8g/dl of agar in the medium) was suitable for the production of the hyphae. The results suggest that the occurrence and recurrence of tinea versicolor in summer probably relate to the change of choleserol concentration on the surface of the skin after the evaporation of sweat.

Objective:To improve effectiveness and sustainability of large-scale instruments and equipment sharing platforms in the TCM (Traditional Chinese Medicine) colleges in China.Methods: Inviting SWOT mode to analyze feasibility of the construction of large-scale instruments and equipment platforms, compares both internal and external factors to find out the former’s strengths and weaknesses the latter’s opportunities and challenges.Results: Building large-scale instruments and equipment sharing platforms from a strategic perspective of onset, adjustment, defense and survival.Conclusion: In order to build effective and sustainable large-scale instruments and equipment sharing platforms, TCM colleges should based on the real conditions, cooperating with the successful scientific research platform ,making an effort to obtain the government's support, Innovating management methods, becoming more open to forming a notion of sharing and collaboration, etc.

Scapulohumeral periarthritis,alternate name‘lujianfeng’or‘jianlingzheng’,is a common disease among the old.Balance acupuncture is a science of modern acupuncture based on the traditional theory of Chinese medicine.In order to sum up the references regarding the clinical treatment features and experimental study of balance acupuncture on scapulohumeral periarthritis in the past few decades.The review shows that as a new modern acupuncture method,balance acupuncture treating scapulohumeral periarthritis is worth to master,popularize to develop and innovate.

Objective To investigate the effect of the balance between helper T lymphocyte (Th) 1 cytokines and Th2 cytokines in the serum of hepatic fibrosis rats induced by CCl4 on the expression of β-catenin in their livers. Methods Seventy-five rats were divided into control group,treatment group and model group. Hepatic fibrosis models were built by subcutaneous injection of CCl4in male Wistar rats, then rats in treatment group were treated with pentoxifylline(PTX) by intragastric administration. The model rats without PTX treatment served as treatment control. The serum levels of Th1 cytokines(interferon-γ, IFN-γ; tumor necrosis factor-α, TNF-α), Th2 cytokines (interleukin-4,IL-4;interleukin-6,IL-6) and type Ⅳ collagen were measured by enzyme-linked immunosorbent assay (ELISA). The expression and distribution of β-catenin in liver tissue and the percentage of area of type Ⅳ collagen accounted in the liver were analyzed by immunohistochemical analyses. The changes in serum cytokines were depicted by using One-way ANOVA, and the correlation between β-catenin expression and cytokines levels or hepatic fibrosis development was elucidated by Bivariate.Results The serum levels of Th2 cytokines in model group were significantly higher than those in treatment group, and even higher than those in control group (F=71. 260,91. 732, all P＜0.05).However, the levels of Th1 cytokines in model group were significantly lower than in treatment group while even lower than those in control group(F= 50. 420,10. 625, all P＜0.05). Serum cytokines came out to be Th2 polarized. Meanwhile, the percentage of type Ⅳ collagen area accounted in the liver,which was corresponding to the degree of liver fibrosis, was lower in the treatment group than the model group and even lower in control group (F=3 000,both P<0.05). β-catenin expression in the liver of model group was stronger than that in the treatment group, and even stronger than that in normal group (F=92. 030, both P＜0. 05). According to β-catenin immunohistochemical analyses based on patho-photos, livers from the model group showed dark color in both cell cytoplasm and nucleus, while the liver from the treatment group only showed weak color in cytoplasm and no straining color in nucleus and the liver from normal group showed colors neither in cytoplasm nor in the nucleus.The expression of β-eatenin had a positive correlation with either the level of Th2 cytokines in serum of rats (r=0. 560,P＜0.01) or the percentage of area of type Ⅳ collagen accounted in the liver of rats (r=0. 757, P＜0. 01). Conclusions Th2 polarization in serum cytokines can aggravate the development of liver fibrosis by inducing the expression of β-catenin, which in the livers of rats is a reflection of the degree of the liver fibrosis.

Objective:To establish early warning mechanism for enhancing the using effect of multimedia and guaranteeing quality of teaching so as to satisfy the requirement of morphology course for definition of multimedia in medical college.Methods: The archive of each multimedia equipment was established and the service times of main components and parts which existed fault were analyzed, and the anticipative occurrence time of fault was pre-deduced. And the early prevention and early detection were implemented in the early warning mechanism.Results: The each multimedia equipment could achieved optimal situation for usage, and got rid of the poor effect when equipment was normal.Conclusion: The warning management of life cycle can fast decrease fault rate and effectively reduce the maintenance cost. Therefore, the mechanism can guarantee the teaching quality of medical college and enhance the satisfaction of teachers and students.

PET-CT is the only current medical imaging technology to provide both metabolic and morphologic information in anatomical configuration mode,with high sensitivity and non-invasion.As one of the optimal measures in tumor diagnosis and therapy,abnormally-increased FDG uptake of some subtypes therefore makes an increasing application of PET-CT in lymphoma.The progress of PET-CT in lymphoma will be summarized in this paper based on the reports from the 58th American Society of Hematology Annual Meeting.

Objective To study the effect of low-dose cyclosporine combined with danazol on chronic aplastic anemia,and to analyze the influence on peripheral hemogram.MethodsRetrospectively analyzed the clinical data of 68 patients with chronic aplastic anemia treated from July 2014 to June 2016 in the second hospital of shandong university,according to their treatment methods were divided into control group and observation group,the control group was given conventional dose of cyclosporine combined with danazol,the observation group received low-dose of cyclosporine combined with danazol,four weeks for a course of treatment.After two courses of treatment,observe the curative effect of two groups,the difference of bone marrow,peripheral blood,immunoglobulin levels before and after treatment were compared in the two groups.Results The effective rate of the observation group was 96.97％,which was significantly higher than that of the control group 77.14％(F=6.563,P=0.028).After treatment,the bone marrow of the observation group was significantly improved compared with the control group(P<0.05); the levels of T-bet,GATA-3 and STAT4 of the observation group were lower than those of control group(P<0.05),the levels of WBC,Hb,PLT and RET were higher in the observation group than in the control group(P<0.05); there was no significant difference in serum immunoglobulin level between the two groups after treatment,there was no significant difference in the incidence of liver and renal function,gastrointestinal reaction,hairy and acne between the two groups.Conclusion Low-dose cyclosporine combined with danazol has better therapeutic effect in the treatment of chronic aplastic anemia,can significantly improve the hemogram and myelogram of patients,has good clinical value high security.

Objective To observe the effects of phenytoin and gabapentin in therapeutic dosage on low-dose veratridine-induced epileptiform discharge in rats' hippocampal CA1 neurons,and explore the involved mechanisms.Methods By means of whole-cell patch clamp technique,the epileptiform discharge model of rats' hippocampal CA1 neurons was constructed with extracellular perfusion of 0.5?mol/L veratridine,and the model should be regarded as successfully estabilshed if bursting discharge emerged within 30min perfusion.The effects of phenytoin(2.5,5,10 and 15?mol/L) and gabapentin(2.5,5 and 10?mol/L) on the epileptiform activity were observed under the voltage-clamp configuration,and the current changes for 1 hour in CA1 neurons was also observed.Results Nine-sixteen minutes after veratridine perfusion,the huge,rhythmic slow oscillation emerged,with 100～200Hz high-frequency discharge,in the hippocampal CA1 neurons,which was similar to the paroxysmal depolarization shifts(PDS),implying that the epileptiform activity was reproduced.Therapeutic dose of phenytoin blocked the veratridine-induced epileptiform activity.The bursting interval of the epileptiform activity was prolonged along with the increased phenytoin concentration,and the duration of bursting was not shortened.1h current decreased gradually in the generation of veratridine-induced epileptiform activity.Therapeutic dose of gabapentin did not block the epileptiform activity in this model.Conclusions In the epileptiform discharge model of rats' hippocampal CA1 neurons,phenytoin can block the epileptic activity in a dose-dependent manner,and the effect may be related to the inhibition of 1h currents.Gabapentin shows no influence on the epileptiform activity,and the possible mechanism may be its ineffectiveness to the persistent sodium currents,and vertridine-induced epileptiform activity does not enhance the 1h currents.

This paper mainly introduces the material composition and structure of two kinds of compound tabletop which currently used in large medical imaging equipment, also introduces the proper material choosing and production processing, together with the advantage and shortage of this two kinds of tabletop and different application occasion.
Construction Materials ; Diagnostic Imaging ; instrumentation ; Durable Medical Equipment

Objective To observe the effects of low dose of veratrine on the discharges of rat hippocampus pyramidal neurons,and to elucidate its possible cytological mechanism.Methods The discharge features of hippocampus CA1 pyramidal neurons of 14-day-aged healthy Sprague-Dawley rats induced by low dose(0.3～0.8?mol/L)of veratrine were observed by slice patch-clamp technique.Presynap- tic stimulation was given to Schaffer collaterals.Presynaptic receptor inhibitors such as 6-cyano-7-nitroquinoxaline-2,3-dione(CNQX, 5?mol/L),DL-2-amino-5-phosphonopentanoic acid(AP-5,12.5?mol/L),bicuculline(Bic,10?mol/L)and tetrodotoxin(TTX,40～80nmol/L)were used to investigate the influence on veratrine-induced epilepsy andⅠ-Ⅴcurves were plotted under these conditions.Elec- trophysiological mechanism of veratrine-induced epilepsy was elucidated on the basis of these experiments,Results After a perfusion with low dose of veratrine,the pyramidal neurons were found to discharge relatively fixed-mode slow wave epileptoid bursts accompanied with hyperpolarization of membrane potential.These epileptoid bursts were not blocked by a mixture of CNQX,AP-5 and Bic,but by low dose of TTX.After a perfusion with veratrine,Ⅰ-Ⅴrelationship tended to be nonlinear and the depolarization rectification was enhanced,which were reversed by administration of low dose of TTX.The subthreshold TTX-sensitive persistent sodium current of CA1 pyramidal cells was enhanced by veratrine in a voltage-dependent manner.Conclusion Inducing slow wave epileptoid bursts,the low dose of veratrine can remarkably change the discharge features of CA1 pyramidal neurons.Such epileptoid activities were not influenced by the synaptic receptor inhibitors,and were obviously related to the persistent sodium current.