Acupuncture Therapy ; Humans ; Male ; Moyamoya Disease ; therapy ; Posterior Cerebral Artery ; abnormalities ; Young Adult

0.05).Conclusion The hypoxic exposure can accelerate the production of erythrocyte, and treat the exercise-induced anemia effectively. Improvement of some haematopoietic factors and enhancement of hematopoiesis in marrow is thought to be the possible mechanism.

Objective To explore the expression of interleukin 13(IL-13) mRNA in peripheral blood mononuclear cells (PBMCs) and its association with serum C-reaction protein(CRP) in patients with ulcerative colitis(UC). Methods IL-13 mRNA expression in PBMCs was detected by RT-PCR in patients with UC(21 cases of active UC and 10 cases of quiescent UC)and 20 healthy subjects. At the same time, the serum level of CRP was detected by ELISA. Results The expression level of IL-13 mRNA in PBMCs of patients with active UC was significantly lower than that in PBMCs of patients with quiescent UC and healthy subjects (6.45?1.23 vs 14.72?2.12 or 15.17?2.38,P0.05). The expression level of IL-13 mRNA in PBMCs of active UC patients was negative correlation with serum level of CRP (r=-0.589,P

Objective:To prepare anti-H pylori immune milk to prevent the infection of H pylori.Methods:The whole active H pylori as immunogen, cows were immunized and the polyclonal immune milk to H pylori was obtained. Results: With direct agglutination test and doubled agar diffusion detection, the titer of antibody was 1:2 048 and 1:32.Conclusion: The prepared immune milk against H pylori has good polyclonal specificity and high antibody titeration.

Clinical experience of professor YANG Mu-xiang in treating hypertension,hyperplasia of prostate gland,acne and thyroid hyperfunction by brunellae spica were introduced,it was considered that the taste of brunellae spica was bitter and sweet,which was not hurt the spleen and stomach,and its cold nature was not consumption of yang,and it could remove hepatic heat,and have the function of eliminating stagnation.It was used when position of disease was liver,and pathogenesis was stagnation of liver qi or accumulation of heat;the dosage of 10-15g was used when the cooling-relieving heat accumulation therapy,and the dosage of 15-30g was used when eliminating stagnation.

Objective To observe effect of Kechuanning on the expression of IL-5mRNA in lung tissue of bronchial asthmatic rats.Methods Forty rats were randomly divided into 5 groups:the normal group,the model group,Kechuanning high-dose group (27 g/kg),Kechuanning low-dose group (13.5 g/kg),Guilong Kechuanning control group (0.45 g/kg),8 rats in each group.The bronchial asthmatic model was established by egg protein sensibilization and inhalation provocation.The rats of each treatment group were lavage administration each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed and lung tissue were taken to dying of HE to be observed.The expression of IL-5mRNA in lung tissue were determined by RT-PCR.Result Compared with the normal group,the thickness of bronchus wall and bronchus smooth muscle,the numbers of eosinophile granulocyte and leukomonocyte,and expression of IL-5mRNA in lung tissue were increased (P

BACKGROUND: The onset of vascular dementia (VD) is closely related to brain ischemic-hypoxia. Previous research has proved that behavioral disturbance can be obviously attenuated by xingnao qizhi capsule in VD rats; however, it remains unclear whether the improvement of VD is due to the protection on ischemic cells.OBJECTIVE: Pheochromocytoma PC12 cells were cultured in vitro and exposed to Na2S2O4 to induce hypoxia injury. Then serum pharmacological technique was used to investigate the protection of xingnao qizhi drug serum on hypoxia-injured PC12 cells.DESIGN: Randomized controlled study.SETTING: School of Traditional Chinese Medicine of Hebei Medical College.PARTICIPANTS: This study was conduced at the Experimental Animal Laboratory and Cell Culture Laboratory, School of Traditional Chinese Medicine of Hebei Medical College, between June and December 2003.Forty SD rats were randomized into 5 groups, namely serum control group (n=12) and xingnao qizhi serum 25.42, 12.71, 6.35 and 3.18 g/kg groups (n=7). PC12 cell line was purchased form the Cell Center of Chinese Academy of Medical Sciences.serum 25.42, 12.71, 6.35 and 3.18 g/kg groups were exposed through gastric lavage to xingnao qizhi of corresponding dosages (composed of wolfberwhile normal saline was given by gastric lavage to rats in control group in dosage of 10 mL/kg, twice a day at an interval of 12 hours for 3 consecutive days. One hour after the last administration, femoral blood samples were collected when rats were deeply anaesthetized and serum was sepainto 6 groups. Cells in control group were cultured with control serum while in experimental model groups cells were exposed to Na2S2O4 to induce hypoxia injury 1 hour later. Cells in Xingnao Qizhi 25.42, 12.71,6.35 and 3.18 g/kg groups were cultured in 5% drug serum with corresponding dosage of xingnao qizhi drug for 1 hour before being exposed to changes of PC12 cells in the six groups were observed under reversal microscope after exposure to Na2S2O4 for 16 hours; the inhibition rate of lactate dehydrogenase production and PC12 cell injury (MTT method) was calculated for assessing cell activity.nase production and PC12 cell injury.of xingnao qizhi displayed better refraction, with fewer cell fragments that tion: It was 81.6%, 69.6%, 54.4% and 27.8% in xingnao qizhi 25.42,PC12 cell injury: It was 82.9%, 75.6%, 65.9% and 53.7% in Xingnao Qizhi 25.42, 12.71, 6.35 and 3.18 g/kg groups, respectively.CONCLUSION: xingnao qizhi drug serum can markedly attenuate hypoxia injury of PC12 cells in dose-dependant manner, which is presented by strengthened cell activity and decreased lactate dehydrogenase activity.

Several biological characteristics of stem cell such as self-renewal and multi-direc tional differentiation,makes stem cell therapy obtain better clinical efficacy in the treatment of many refractory diseases,Even some of the incurable disease in the treatment of the disease.Stem cell therapy showes a good prospect,brings the clinicians and patients in despair with confidence and hopesometimes.This article introduced the stem cell treatment policy,the management system and the present situation in domestic and foreign,pointed out the main problems of stem cell therapy in china and discussed on the dispute of stem cell therapy in scientific research management and clinical application in China.

Aim To study the differentiation of human bone marrow-derived mesenchymal stem cells ( HM-SCs) into retinal cells in vitro. Methods HMSCs were isolated from human bone marrow after Ficoll den-sity gradient centrifugation. The adherent cells after at least 5 passages were used for study. Immunopheno-type of the cells was analysed by flow cytometer, and cellular differentiation was identified by immunofluores-cence labeling technique. Results The target cells derived from human bone marrow adhered to the plate with fibroblastic-like morphology, whose surface mark-ers were similar to mesenchymal stem cells. Major cells
were positive for CD90 , CD44 , CD147 , while they were all negative for CD34, CD45, HLA-DR. In the differentiation study, HMSCs cultured in induced me-dium can differentiate into nestin ( neural stem cell ) -positive cell, GFAP ( glial fibrillary acidic protein ) -positive glial cells and retina-specific neurons express-ing Rhodopsin with CD90 ( mesenchymal stem cells )-negative. Conclusion HMSCs have the ability to dif-ferentiate into retinal neural cells in vitro.

Objective To evaluate the value of virtual touch tissue imaging quantification (VTIQ),which was combined with ultrasound Breast Imaging Reporting and Data System (BI-RADS),for differentiating benign from malignant breast lesions.Methods Totally 190 patients with 214 breast lesions were enrolled in this study.All lesions were classified according to the BI-RADS for sonography.The maximum,minimum and mean shear wave velocity (SWV) values on VTIQ in the lesions were obtained.The SWV between benign and malignant lesions were analyzed.Receiver operating characteristic(ROC) curves were plotted to determine the cut off value of VTIQ for differential diagnosis of breast lesions. Results Pathology confirmed 166 benign lesions and 48 malignant lesions.The SWVmax,SWVmin,SWVmean of malignant lesions were significantly higher than those of benign lesions (all P<0.001).The SWVmean 3.67 m/s as the best value in the diagnosis of benign and malignant lesions.The sensitivity,specificity,positive predictive value and negative predictive value of the combination of the SWVmean and BI-RADS classification standard in the diagnosis of benign and malignant breast lesions were 77.6%,97.5%,92.7%,91.3%,respectively.Conclusions The combination of VTIQ and ultrasonic BI-RADS can improve the ability of differential diagnosis of benign and malignant breast lesions.