Objective To establish the HPLC fingerprint of methanol-resoluble component in Radix Gentianae collected from different habitats and accordingly offer the evidence for quality control and quality evaluation of Radix Gentianae.Methods Ten batches of Radix Gentianae were measured by the RP-HPLC,and their fingerprints were obtained.Chromatographic condition: ODS-column,gradient elution with methanol-water,the detection wavelength was at 254 nm,flow rate was at 1.0 mL/min,and the column temperature was 25 ℃.Results The mutual mode of Radix Gentianae was set up and then the similarity was compared with software which was published by the Committee of Pharmacopeia of China.Conclusion According to the analysis of statistics,this method is steady,accurate with good repeatability,and can be used as standardization implantation and quality control of Radix Gentianae.

OBJECTIVETo establish a RRLC-UV method for simultaneous determination of nine phenolic acids in Salvia miltiorrhiza.

METHOD3,4-Dihydroxyphenylacetic acid was used as internal standard, and analysis was carried out on an Agilent Zorbax Eclipse Plus C18 (2.1 mm x 50 mm, 1.8 microm) column with acetonitrile-0.1% formic acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.3 mL x min(-1), and the UV detector was monitored at 286 nm.

RESULTAll calibration curves showed good linear regression within test ranges (r > or = 0.999 5); and the overall recoveries were in the range of 98.2%-102.6%, with RSD less than 3.1% (n = 3). The overall RSD of precision test were less than 2.4%.

CONCLUSIONThe developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of S. miltiorrhiza.

Chromatography, High Pressure Liquid ; instrumentation ; methods ; Drugs, Chinese Herbal ; analysis ; Hydroxybenzoates ; analysis ; Salvia miltiorrhiza ; chemistry ; Spectrophotometry, Ultraviolet

OBJECTIVETo establish a HPLC method to determine the carnosic acid in the stomach and intestine of rats and study its tissue distribution characteristics.

METHODAfter intragastric administration of carnosic acid (90 mg x kg(-1)), rats for each time-point were sacrificed by decapitation. After removal of the blood, various tissues were rapidly removed and weighted, all tissues were treated with a series of pretreatment before HPLC. Chromatographic separation was achieved on a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) protected by an ODS guard column at 25 degrees C, using acetonitrile-0.1% phosphoric acid solution (55:45) as mobile phase, at a flow rate of 1 mL x min(-1). The wavelength of the UV detector was set at 210 nm for carnosic acid and internal standard.

RESULTGood linearities were obtained in every tissue over a range of 0.3212-160.6 mg x L(-1). The recovery, intra-day and inter-day precision and accuracy of three concentrations of carnosic acid in tissues met the requirements of methodology. And the stability of the tissue samples were also validated. The results of distribution in stomach and intestine showed that the highest concentration was (307.1 +/- 119.2) microg x g(-1) in stomach and (33.32 +/- 17.70) microg x g(-1) in intestine after intragastric administration of carnosic acid.

CONCLUSIONThe HPLC method was established to determine the concentration of carnosic acid in tissues. This method is quick, precise, and reproducible. It is the first time to study the tissue distribution of carnosic acid in rats after intragastric administration.

Animals ; Antioxidants ; pharmacokinetics ; Calibration ; Chromatography, High Pressure Liquid ; Diterpenes, Abietane ; pharmacokinetics ; Intestines ; metabolism ; Linear Models ; Male ; Plant Extracts ; pharmacokinetics ; Rats ; Sensitivity and Specificity ; Stomach ; metabolism ; Time Factors ; Tissue Distribution