Objective:To investigate the work requirement and training needs of general practitioners in Shenzhen during the outbreak of coronal virus disease 2019 (COVID-19).Methods:From February 19 to February 29, 2020, the questionnaires of "Coping with COVID-19 epidemic of genereal practitioners in Shenzhen region" were distributed to 4 002 general practitioners in Shenzhen hospitals and communities through the Questionnaire Star software. The changes of job content, education parttern, and the perception of roles before and during the epidemic were surveyed; the different roles of doctors engaged in general medicine before and after 2008 in education and community work were compared; the needs for training in epidemic prevention related knowledge for general practitioners were investigated.Results:A total of 382 questionnaires were recovered, of which 361 were valid, and the effective rate was 94.5%. The 83.1% (300/361) of general practitioners believed that the number of patients during the epidemic was significantly reduced. The main work was to screen community residents in epidemic areas (68.9%, 249/361), telephone counseling (54.0%, 195/361), and screening and follow-up of close contacts (42.9%, 155/361); 98.9% (357/361) of community doctors participated in outpatient education. Experienced doctors (starting work before 2008) were more willing to undertake management work in the epidemic (χ 2=77.370, P<0.05) . General practitioners′ needs for training were psychological interventions for residents under quarantine (80.6%, 291/361), home protection knowledge in the community (74.2%, 268/361), and isolation measures of the family with suspected patients (67.3%, 243/361), wearing and taking off protective gowns (65.1%, 235/361), collecting pharyngeal swab specimens (57.3%, 207/361) and patient referral procedures (52.4%, 189/361). The 60.6% (219/316) of general practitioners preferred information from the government press conference, expert opinion or the intranet of the organization (such as the hospital management department). Conclusion:General practitioners in Shenzhen took important screening, public education and counseling works during the COVID-19 epidemic, meanwile the training in the psychological intervention, the prevention and control knowledge related to the epidemic should be strengthend to better cope with the epidemics.

Organ transplantation is an effective treatment for end-stage organ failure. However, organ shortage has always been a common problem faced by countries around the world. The recognition and active participation of intensive care unit (ICU) medical staff in organ donation contributes to promoting the development of organ donation, thereby alleviating the shortage of donor organ. In this article, the key strategies of ICU donor management to promote organ donation and the key strategies of ICU medical staff management to promote organ donation were summarized, aiming to provide reference for organ donation practitioners (especially ICU medical staff) and jointly facilitate the professional development of organ donation.

Objective At present, the novel coronavirus pneumonia (COVID-19) pandemic is still raging in certain regions around the globe, and the prevention and control of the pandemic should be strengthened. Under the challenges of respective social environment and allocation of medical resources, and support from the inertia and inherent productivity of the system on which the industry depends, extensive attempts are being delivered to push forward the work of organ donation and transplantation in each country. Under the guidance of national experts and committee members, Shanxi Provincial Human Organ Procurement and Allocation Service Center was established on August 28, 2018 approved by the former Shanxi Provincial Health and Family Planning Commission. It is the only independent non-profit medical institution in Shanxi Province. In this article, the system construction of citizen's organ donation and transplantation fitting national and provincial conditions was further explored according to the data analysis of organ donation and transplantation in the United States and Spain during the COVID-19 pandemic combined with the implementation of organ donation work in Shanxi Provincial Human Organ Procurement and Allocation Service Center.

Huntington's disease (HD) is a deadly neurodegenerative disease with abnormal expansion of CAG repeats in the huntingtin gene. Mutant Huntingtin protein (mHTT) forms abnormal aggregates and intranuclear inclusions in specific neurons, resulting in cell death. Here, we tested the ability of a natural heat-shock protein 90 inhibitor, Gedunin, to degrade transfected mHTT in Neuro-2a cells and endogenous mHTT aggregates and intranuclear inclusions in both fibroblasts from HD patients and neurons derived from induced pluripotent stem cells from patients. Our data showed that Gedunin treatment degraded transfected mHTT in Neuro-2a cells, endogenous mHTT aggregates and intranuclear inclusions in fibroblasts from HD patients, and in neurons derived from induced pluripotent stem cells from patients in a dose- and time-dependent manner, and its activity depended on the proteasomal pathway rather than the autophagy route. These findings also showed that although Gedunin degraded abnormal mHTT aggregates and intranuclear inclusions in cells from HD patient, it did not affect normal cells, thus providing a new perspective for using Gedunin to treat HD.