OBJECTIVE: To establish an HPLC method for the determination of Baicalin in Tanreqing injection.METHODS: The determination was performed by HPLC using an CLC-ODS C18 column(150 mm?6.0 mm,5 ?m),with mobile phase consisted of methanol-water(47∶53) at a flow rate of 0.5 mL?min-1 and a detection wavelength of 280 nm.RESULTS: The calibration curve for Baicalin was linear in the concentration range of 2.53～12.65 ?g?mL-1(r=0.999 8).The average recovery was 99.61%(RSD=0.35%,n=9).CONCLUSION: The method is simple,rapid and applicable for the quality control of Tanreqing injection.

OBJECTIVE:To establish the HPLC method for determination of tanshinone Ⅱ A in compound Jinpu tablets METHODS:HPLC was performed on Shim-pack CLC-ODS(4 6mm?250mm,5?m) with methanol-water(15∶5) as mobile phase Flow rate was 1 5ml/min and detecting wavelength was 270nm Tanshinone Ⅱ A in compound Jinpu tablets was extracted by reflux method RESULTS:The calibration curve was linear in the concentration range of 0 01 333～0 10 667?g/ml(r=0 9 998,n=5) CONCLUSION:This method is simple,accurate,repeatable and effective for quality control of compound Jinpu tablets

OBJECTIVE:To establish HPLC method for the determination the centent of tanshinone Ⅱ_A in danshen shuxin capsule.METHODS:The determination was performed on Shim-pack CLC-ODS column,the mobile phase consisted of methanol-water(15∶5),the detection wavelength of270nm,column temperature at40℃,a flow rate of1.5ml/min and.RE_ SULTS:The calibration curve of tanshinone Ⅱ_A is linear when its concentration range was with in 0.05125～0.25625?g/ml(r=0.9996,n=5),the average recovery was 99.05%(RSD=0.92%).CONCLUSION:This method is convenient in operation,precise,reliable in results,and it can be used as quality control for danshen shuxin capsule.

Objective To observe the effect of electroacupuncture on onset time and effectiveness of Paroxetine for mild or moderate depression patients and on the scores of Hamilton depression rating scale for depression(HAMD) and self-rating depression scale(SDS). Methods Fifty-five patients with mild or moderate depression were randomly divided into A group( Paroxetine group,29 patients) and B group (Electroacupuncture combining with Paroxetine group,26 patients). Therapeutic periods for the two groups were 6 weeks. HAMD and SDS were used to evaluate the efficacy before the research and after treatment on 1 week,2 weeks,4 weeks,6 weeks.Results HAMD scores and SDS scores for A group were significant difference after 2 weeks treatment compared with the beginning of the research (P＜ 0.05 ) ;HAMD scores and SDS scores for B group were significant difference after 1 week treatment compared with the beginning of the research(P＜0. 05 ). Total effective rate of HAMD for A group was 71.4% ,total effective rate of SDS for A group was 75% ,total effective rate of HAMD for B group was 88% ,total effective rate of SDS for B group was 84%. The total effective rate between the two groups ordered by the individual chi-square test was significant difference( X2 = 4. 1790, P= 0. 0408). Conclusion Electroacupuncture can shorten the onset time and increase the effectiveness of paroxetine for mild or moderate depression patients.

Low-frequency ultrasound can increase the transdermal delivery of many drugs, including macromolecular drugs. The main mechanism is ultrasonic cavitation. Most researchers pointed out that it could change the form-structure of stratum corneum keratinocytes and, in this way, it can improve the permeability of skin. Low-frequency sonophoresis has been in use for in-vitro experiments and in-vivo animal experiments, and so far, both small-molecules transdermal delivery and macromolecules transdermal delivery have been successfully performed in many experiments. However, there are few reports about the real low-frequency sonophoresis for clinical treatment. A large number of clinical trials are necessary to confirm its safety and practicality. Once its safety is confirmed and the suitable low-frequency sonophoresis devices are developed successfully, Low-frequency Sonophoresis will come to be a safe, effective, controllable, and economic new delivery method.
Administration, Cutaneous ; Animals ; Humans ; Skin ; diagnostic imaging ; metabolism ; Skin Absorption ; radiation effects ; Ultrasonography

Objective: To discuss the strategy of therapeutic management of T3 supraglottic carcinoma. Methods: A retrospective analysis of 459 patients with T3 supraglottic carcinoma treated in our hospital was performed. We evaluated the results of different managements, including surgery alone, preoperative radiotherapy, postoperative radiotherapy and radiotherapy alone. The extent of the lesion was also put into analysis. Statistical analysis of the overall survival (OS), cause-specific survival (CSS), local control (LC), regional control(RC), function-conservation (FC) were performed with the statistical package from SPSS. Results: In all patients, the rates of 5-year OS, CSS, LC, RC and FC were 64.2%, 71.2%, 87.8%, 78.8% and 64.5% respectively. The OS, LC and FC of the patients treated by surgery alone, preoperative radiotherapy and postoperative radiotherapy had no significant difference, and were remarkably better than that of patients treated by radiotherapy alone (P<0.001). In 412 patients treated by surgery, 300 patients received function-conservation laryngectomy. 209 patients (50.7%, 209/412) survived and maintained well-function of larynx for 5 years, which was significantly better than those in the radiotherapy alone group (27.7%, 13/47). The patients with the lesion invading the pre-epiglottic space but limited in supraglottic area had better OS (70.2%), LC (93.5%) and FC (85.1%). The rate of 5-year neck lymphatic metastasis was 56.2%(258/459), and the 5-year OS of patients with N0, N1, N2 and N3 stage were 76.0%, 66.2%, 50.5% and 13.0% respectively. Conclusions Surgical treatment was the best therapeutic approach for T3 supraglottic laryngeal carcinoma. Most patients with T3 lesions are suitable for function-conservation laryngectomy. Surgical procedure was determined by tumor invaded location and extension. The combined therapy of surgery and radiotherapy had no significant advantage.