Objective:To investigate the prevalence and related factors of dental caries in 3-year-old children in the Development District in Dalian.Methods:A multi-stage,randomized,cluster sampling method was used in the cross-sectional study.All 3-year-old children in the selected 15 kindergartens were invited to take part in the oral health examination conducted by 3 calibrated examiners.The WHO recommended caries diagnosis criteria were used.The structured questionnaire including the information of children's oral hygiene habits,dietary habits,parents' oral health knowledge and socioeconomic status was completed by the parents.Results:1 220 3-year-old children completed the clinical oral examination and 964 children's parents(79.0％) finished the questionnaire.The caries prevalence in 3-year-old children was 62.8％,and the mean dmft and dmfs scores were 3.40 and 7.34,respectively.No differences in the prevalence of caries,mean dmft and dmfs scores were found between boys and girls(P ＞0.05).Logistic regression analysis showed that the frequency of sweets and sugar-containing beverages consumption(P＜0.05),parents'education level(P＜0.05) were significantly related to the caries prevalence.Conclusion:The prevalence and severity of dental caries in 3-year-old children in Dalian Development District were high and the frequency of consumption sugar-containing foods and beverages,parents'education level were related to the decay prevalence.

Objective To study the relationship between handgrip level and characteristics of surface electromyogram(sEMG)signal from forearm muscles.Methods Nine healthy volunteers were asked to do a specific grip test with three force levels of maximum volunteer contraction(MVC),80% MVC and 40% MVC.sEMG signal was recorded from flex digitorum superficial(FDS)and extensor carpi radialis longus(ECRL)simultaneously.The amplitude characteristics of sEMG signal were calculated by the means of root mean square(RMS)and wavelet analysis.Furthermore,to compare the relationship of sEMG and force level between different participants,SPSS was applied to analyze the relative value that was defined as the ratio between sEMG characteristics of MVC condition and that of different grip levels.Results There existed positive correlation between grip level and sEMG activity,stronger grip corresponded the greater sEMG characteristics for every participant,and this correlation revealed significant coherence between different volunteers.Conclusion There is significant correlation between grip force and forearm muscle activities represented as sEMG characteristics.The result suggests that sEMG can be used to predict grip force noninvasively for measuring motor function and assessing the motor function rehabilitation.

AIM: To investigate the effects of salidroside on intracellular free calcium concentration (([Ca~(2+)]i)), apoptosis, mitochondrial membrane potential (MMP) and activity during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells. METHODS: Mitochondrial activity was measured by methylthiazolyl tetrazolium test. MMP, [Ca~(2+)]i and apoptosis were measured by flow cytometry. RESULTS: SH-SY5Y cells were cultured in a hypoxia/hypoglycemia condition for 2, 4, 6 and 12 h, [Ca~(2+)]i and apoptosis rate significantly increased compared with control group (P

OBJECTIVE:To study the curative effects of Shenyanqing tablet.METHODS:1 200 patients with chronic glomerulonephritis,acute glomerulonephritis,latent glomerulonephritis and purpura glomerulonephritis were randomized into the treatment group and control group,and separately studied on curative effects and reduction of adverse reaction of glucocorticosteroid.In the study of curative effects,the control group received no special treatment except that on reducing pressure and diuresis,and the treatment group was given Shenyanqing tablet,8 tablets? d-1,tid,in addition to receiving same treatment as that for the control group.In the study of reducing adverse reaction of glucocorticosteroid,the control group(the treatment group was the same as the method above)was given normal glucocorticosteroid treatment for 3 months and 2 months respectively.RESULTS:It has significant difference(P

Objective To understand the distribution of pathogenic genes in uropathogenic Esche-richia coli (UPEC) from urinary specimen and to analyze the pathogenicity of UPEC and their mechanism of apoptosis to HeLa cells. Methods We have analyzed 6 pathogenic genes among the 28 strains of the clini-cally isolated E. coli from urinary tract infected patients. The 6 pathogenic genes were surveyed by using the PCR amplification of the target genes. The adhesion experiments and tryphan-blue staining was used to screen the phenotype of the pathogenic strains, while Annexin V/PI method was applied to study the strains to cause apoptosis of the HeLa cells, which was further confirmed with electronic microscopy. Results We have detected 6 strains that carried the usp gene. Phenotype screening identified two high virulent isolates (strain 6N and 27N) from the 6 strains. Strain 27N and 6N contained very similar virulence gene profile ex-cept that strain 27N did not contain usp gene. Both strains can destroy HeLa cell within 3 hours causing cell death. Results of apoptosis detected by flow cytometry revealed that strain 6N induced 20.75% of HeLa cells to an early stage apoptosis within 1.5 hours. On the other hand, strain 27N induced only 1.55% of HeLa cells to apeptosis. Conclusion High virulent UPEC strain carrying usp gene can induce HeLa cell rapid early apoptosis.

Objective To evaluate the effectiveness of resin-based sealants and glass ionomer sealants on caries prevention in the first permanent molars.Methods A randomized controlled,examiner-blinded,2-year clinical trial with parallel groups was conducted.A total of 419 children(664 first molars) who had at least one sound first permanent molar with deep fissures or fissures with signs of non-cavitated lesions were recruited.They were randomly allocated into three groups.Helioseal F,a light-cured resin-based sealants was used in group R (136 children with 219 first molars).FUJI Ⅶ,a light-cured glass ionomer sealants was used in group G(130 children with 218 first molars) and group N (153 children with 227 first molars) was blank control.Follow-up examinations were conducted at 6,12,and 24 month after sealing to examine the sealants retention and caries incidence.Chi-square test was used to compare the differences of caries incidence among the 3 groups.Generalized estimating equations (GEE) was used to analyze the effective factors of caries incidence.Results During the 2-year follow-up period,3.0％(6/197) of the teeth in group R,6.7％ (13/193) of the teeth in group G,and 14.7％ (29/197) of the teeth in group N were diagnosed as having developed a cavitated lesion.The difference in the incidence of caries among the three groups was significant (P ＜ 0.05).No significant difference in caries incidence was found between group R and group G (P ＞ 0.05).The tooth-level preventive fractions were 79.6％ for resin-based sealants and 54.4％ for glass ionomer sealants,respectively.Conclusions Both resin-based sealants and glass ionomer sealants can effectively prevent the incidence of caries in the first permanent molars.The two sealants have similar caries prevention effect

Objective:To assess the relationship between the characteristic of drug resistance in Acinetobacter baumannii and the syndrome of traditional Chinese medicine(TCM) in intensive care unit(ICU).Methods:Sixty-six strains of Acinetobacter baumannii were isolated from sputum specimens of patients in our ICU from March 2005 to February 2006.The data of the drug sensitivity test in vitro was analyzed.The relation between the syndrome of TCM and drug resistance in Acinetobacter baumannii was probed.Results:The 66 strains of Acinetobacter baumannii were drug resistant to multiple kinds of anti-bacterial drugs(sensitivity rate

Objective To explore the influence factors on hepatitis B virus (HBV) relapse after nucleos(t)ide analogues (NA) withdrawal in the chronic hepatitis B (CHB) patients who met NA cessation criteria. Methods Eighty-one consecutive CHB patients were treated with NA, 38 with lamivudine (LAM), 25 with adefovir dipivoxil (ADV), 12 with entecavir (ETV), 6 with LAM +ADV. Among recruited patients, 40 were hepatitis B virus e antigen (HBeAg) positive, 41 were HBeAg negative, 67 of them were initial treatment, 14 were retreatment due to resistance to NA at baseline. The treatment was discontinued after meeting China therapeutic end-point criteria. HBV DNA, HBV serological markers, alanine aminotransferase (ALT) were measured respectively at baseline, every month before virological response, every 3 months after virological response, every month within first 6 months and every 2 months over 6 months after drugs withdrawal. Twelve probable influence factors on relapse which were sex, age, HBV family history, baseline HBV DNA,baseline HBeAg status, baseline ALT, virological response time, total duration of treatment, duration of additional treatment, the level of hepatitis B virus surface antigen (HBsAg) at cessation therapy,initial treatment or retreatment, drug category were analyzed with univariate, multivariate Cox regression modle and stratified analysis. The cumulative relapse was calculated by the Kaplan-Meier method. Results A total of 36 patients (44. 4%) relapsed within 1 year. Initial treatment or retreatment, HBV family history, virological response time, the level of HBsAg at cessation therapy were independent risk factors. The relapse rate of retreatment was higher than that of initial treatment (78.6% vs 37. 3% , χ2 = 7. 983, P = 0. 005) , those of patients with HBV family history higher than without family history (64. 5% vs 15.0%, χ2 =12. 096,P = 0.002), those of patients obtained virological response within 3 months lower than after 3 months(34. 0% vs 64. 3% , χ2 =6. 823,P=0. 009) , those of patients with HBsAg≤150 μg/L at cessation therapy lower than >150 μg/L(27. 6% vs 53. 8%, χ2=5. 199,P=0. 023). Conclusions Retreatment, HBV family history, later virological response and higher HBsAg level at cessation therapy are risk factors of relapse after NA withdrawal. Such patients should be treated with prolonged duration after meeting end-point criteria to strengthen the efficacy.

Objective:To understand the oral health status of urban and rural residents in China,the Fourth National Oral Health Epidemiological Survey (FNOHES) was conducted in 2015.Materials and methods:Based on the principles of multi-stage,stratified,equal capacity,and random sampling,the subjects included five groups of residents aged 3-5,12-15,35-44,55-64,and 65-74 years in all 31 provinces,municipalities,and autonomous regions except Hong Kong,Macao,and Taiwan.The total sample size was 172,425.The survey covered two areas,namely oral health status and knowledge,attitude and oral health behavior.Results:Residents in China had serious caries and poor periodontal condition.The prevalence of caries was above 50％ in all age groups,subjects without periodontal disease accounted for ＜50％,and fewer than 50％ of subjects brushed their teeth twice a day.Based on the results of the FNOHES,the following suggestions were proposed for improving the oral health in China in the future:to establish and improve the comprehensive prevention and control system for oral diseases,implement a comprehensive prevention and control strategy for priority groups,strengthen the oral health education for all people to improve oral health literacy,enhance dynamic monitoring to scientifically evaluate oral health status,and coordinate multi-party resources to establish and improve the oral health service security system.Conclusion:The oral health status of Chinese residents and their oral health behavior habits need to be improved.With the support of the administrative departments of the government and unremitting efforts of the oral health staff,all sectors of society must be coordinated to strengthen oral health education for the whole population.Furthermore,comprehensive prevention and control measures must be implemented for priority groups.

Objective This study aims to determine the fissure morphology, prevalence, and severity of caries in the first permanent molars of 7-year-old to 9-year-old children in the Dalian development area. The study also aims to investigate the relationship between fissure morphology and noncavitated lesions. Methods A multi-stage, cluster sampling method was used. Three schools were randomly selected from 20 elementary schools in the Dalian development area. All of the children in the selected schools completed clinical oral examinations. A diagnosis criterion of the International Caries Detection and Assessment System was used to assess the cavitated and noncavitated dental caries in the first permanent molars. Fissure depth and fissure stain were also assessed. Results The total size of the study sample was 1 160. The prevalence of cavitated caries was 19.1%, and the mean D3-6FT score was 0.31. No significant differences were found between urban and rural sub-jects, between men and women, and among different age groups. The prevalence and D1-2FT score of noncavitated caries were 42.1% and 0.86, respectively. The D1-2FT score for rural children was significantly higher than that for urban children, and the D1-2FT score for girls were higher than that for boys. No statistical differences were found among different age groups. The prevalence of deep fissures in the first permanent molars was 44.7%. The prevalence of noncavitated caries increased with increasing fissure depth. Conclusion The prevalences of noncavitated caries and deep fissure in the first permanent molars of 7-year-old to 9-year-old children in Dalian were high. Fissure morphology was found to be related to noncavitated caries.