OBJECTIVE: To study the compatibility stability of Chinese material medica injection for reference of clinical rational administration. METHODS: The pertinent literature about the compatibility stabililty of Chinese material medica injection during 1999-2006 retrieved from CHKD were statistically analyzed. RESULTS: The analysis on temperature, pH, corpuscle and concentration et exactly and directly revealed that Chinese material medica injection was stable under any infusion condition, which serves as reference for clinical medication. CONCLUTION: Because of the complexity of the components and process of preparation or extraction, the Chinese material medica injection often induce adverce reaction during intervenous drop infusion, thus great importance should be attached to the compatibility stability in centralized pharmacy admixture to ensure the clinical medication safety and decrease the incidence of adverse drug reaction.

Objective To analyze the surgical management and prognosis of hilar cholangiocarcinoma(HCC).MethodClinical data of 72 patients with HCC were retrospectively analyzed.Results There was a significant difference in the 3- and 5- year survival rates and median survivals among the groups treated with different surgical methods(x2 =28.1,P＜ 0.01 ).Univariate analyses conducted for the 16 factors which might affect prognosis showed the surgical method,lymphatic metastasis,positive margin,as well as the type of pathology and staging were of statistically significant prognostic value (P＜0.05).On a multivariate analysis using the Cox proportional hazards model,surgical management,lymphatic metastasis and the type of pathology and staging were independent prognostic factors (regression coefficient 1.4157,1.1072,1.6435).ConclusionRadical excision is an important prognostic factor for HCCa and extended resection improved the long-term survival rates of radical surgery.

OBJECTIVE:To establish the HPLC method for determination of tanshinone Ⅱ A in compound Jinpu tablets METHODS:HPLC was performed on Shim-pack CLC-ODS(4 6mm?250mm,5?m) with methanol-water(15∶5) as mobile phase Flow rate was 1 5ml/min and detecting wavelength was 270nm Tanshinone Ⅱ A in compound Jinpu tablets was extracted by reflux method RESULTS:The calibration curve was linear in the concentration range of 0 01 333～0 10 667?g/ml(r=0 9 998,n=5) CONCLUSION:This method is simple,accurate,repeatable and effective for quality control of compound Jinpu tablets

Objective To investigate the application values of thymosin α1 in severe sepsis.Methods Selected 94 patients with severe sepsis in our hospital from September 2014 to October 2016,were randomly divided into observation group(n=45)and control group(n=49),the control group was given routine treatment,the observation group was given thymosin α1 on the basis of conventional treatment,observed two groups before and after treatment T cell subsets and CD14+monocyte human leukocyte antigen(HLA-DR)and so on.Results The observation group the duration of ventilator use and ICU treatment were(12.51±3.82)d and(15.81±3.18)d,significantly shorter than the control group(P< 0.05).There was no significant difference in 28d mortality between the observation group and the control group; After treatment,the CD14+monocytes HLA-DR,CD3+and CD4+T cells in the observation group were significantly improved than before treatment(P<0.05);The HLA-DR,CD3+and CD4+T cells in the observation group after treatment were(36.04± 8.90)％,(58.93±8.74)％and(43.20±9.90)％,significantly higher than those in the control group(P<0.05);The observation group after treatment TNF-α,endotoxin and CRP respectively(56.40±11.78)ng/L,(27.83±9.98)ng/L and(53.20±9.73)g/mL,significantly lower than the control group(P< 0.05).Conclusion Application of thymosin α1 in the treatment of severe sepsis,which can improve the cellular immune function,adjust the state of inflammatory response,shorten the duration of mechanical ventilation and ICU stay.

Objective: To study the role of IL-6 in the dental pulp indirect injury by gunshot. Methods: The mandible was wounded by gunshot at (961.18?8.94) m/s in 17 dogs.2 h,6 h,1 d,3 d,7 d after injury molars,caspids,and lateral incisors were sampled respectively for IL-6 detection by immunohistochemical method in dental pulp. Results: The results showed that positive staining of IL-6 was observed predominantly in infiltrating inflammatory cells, vascular endothelial cells and some fibroblasts,IL-6 expression was consistent with the recruitment and activation of inflammatory cells, and with the severity duration of pulpal tissue injury and response. IL-6 level was increased in the dental pulp 6 h～3 d after injury.Conclusion: IL-6 is involved in regulating physiologic responses of dental pulp injuries in maxillofacial trauma by a projectile.

Objective To study the growth inhibition of human hepatoma cells tranfected with type Ⅱ insulin like growth factor receptor (IGF ⅡR) antisense gene. Methods We constructed the recombinant eucaryotic expression plasmids of IGF ⅡR sense and antisense genes, which were transfected into SMMC 7721 human hepatoma cell line using calcium phosphate coprecipitation, and selected in DMEM supplemented with 500 ?g/ml G418(Geneticin) for 2～3 weeks. Then the total number of colonies formed was counted. The effect of IGF ⅡR antisense gene transfection on endogenous IGF ⅡR mRNA levels was examined by Northern blot, and the growth rate and the ability of the transfected cells to form colonies in soft agar medium were also examined. Results The hepatoma cells transfected with IGF ⅡR antisense gene exhibited significant reduction in endogenous IGF ⅡR messenger RNA(mRNA) levels and loss of their ability for anchorage independent growth in soft agar. The IGF ⅡR antisense gene supressed the formation of colonies of SMMC 7721 cells resistant to G418 without alteration on cell growth curve. Conclusion The IGF ⅡR antisense gene expression effectively blocks IGF Ⅱ to IGF ⅡR autocrine and/or paracrine signal transduction pathway and reverses certain aspects of the cell malignant phenotype.

本文在“国际残疾分类”的基础上，围绕康复诊断名称的标准化，进行了初步探讨，力求使之适合于临床康复的应用及康复病案管理。

Objective To apply the oral gastrointestinal ultrasound contrast agents for evaluating the gastric motility abnor‐mality in the patients with anxiety disorder .Methods Twenty patients with anxiety disorder complicating upper digestive tract symptoms without organic pathological changes were enrolled as the anxiety disorder group .Twenty healthy volunteers were en‐rolled as the control group in this study .The two groups orally took gastrointestinal ultrasound contrast agents .The antral contrac‐tion frequency ,antral contraction amplitude amd MI were measured at each time point .GER was calculated .The the gastric motility parameters in the patients with anxiety disorder were evaluated .Results The antral contraction frequency and MI at initial 2 min had no statistical difference between the anxiety group and the control group .The antral contraction amplitude ,antral contraction frequency amd MI at each time point during 5 -10 min after contrast in the anxiety disorder group were significantly decreased compared with the control group ,and the differences were statistically significant (P< 0 .05) .After 20 min ,GER in the control group was significantly higher than that in the anxiety group ,the difference was statistically significant (P<0 .05) .Conclusion O‐ral gastrointestinal ultrasound contrast agents is a economic ,strongly operable ,non‐invasive and highly repeatable method for evalu‐ating the gastric motility .

OBJECTIVE: Amyotrophic lateral sclerosis(ALS) is a chronic progressive degenerative disease selectively infringing upper and lower motoneurons, and its pathogeny and pathogenesis are unclear till now. Its researches are mainly relying on animal models. Currently, there are many methods for the establishment of ALS animal.model and each of them has its own characters,practicability and values.DATA SOURCES: Relative articles during the period from January 1980 to December 2003 were searched on the Medline database by the title of the article with the searching word "amyotrophic lateral sclerosis, model" and the language of the articles was limited to English.STUDY SELECTION: Data were preliminarily screened for the selection of the literatures mainly regarding the researches of ALS animal models; and then literatures regarding the application of ALS animal model in medication effectiveness and pathogenesis were eliminated; the full texts of the rest literatures were searched as inclusive criteria.DATA EXTRACTION: Totally 96articles searched with "amyotrophic lateral sclerosis, model" in title were collected. Fifteen tests were in accordance with the inclusive criteria. In the eliminated 81 tests, 75 literatures were about the researches on the application of ALS model for medication observation and pathogenesis research, and the rest 6 articles were repetitive experiments.DATA SYNTHESIS: ALS neurotoxic animal model, immune mediated animal model, spontaneous animal model, and transgenic animal model were established in the selected literatures, which also investigated the mechanism, features, practicability and value of the animal model.CONCLUSION: The establishment of animal models simulated ALS symptoms or pathogenesis in the above literatures can be used in ALS disease researches as referencing model, which could provide animal experimental basis for the researches of ALS pathogenesis and the confirmation of therapy plan.

Objective To investigate the imbalance of T helper 17 (Th17) / CD4 + CD25 + regulatory cell (Treg) in HAM/TSP patients.Methods Enzyme linked immunosorbent assay (ELISA) was performed to measure the levels of interleukin (IL-17) and IL-10 in CSF,and flow cytometry to determine the percentage of Th17 and Treg cells in peripheral blood of patients with HAM/TSP.Results There was a significant increase in the level of IL-17 [(4.58 ± 0.70)pg/ml vs (0.76 ± 0.17)pg/ml,P ＜ 0.01] in CSF and the percentage of Th17 cells [(2.00 ± 0.64) ％ vs (0.41 ± 0.24) ％,P ＜ 0.01],but a decrease in the level of IL-10 in CSF and the percentage of Treg cells in peripheral blood of patients with HAM/TSP compared with the control group.The ratio of Th17/Treg cell (0.55 ±0.10 vs 0.06 ±0.03) in the peripheral blood of patients with HAM/TSP increased significantly compared with control group (t =12.11,P ＜0.01).Conclusions The imbalance between Thl7 and Treg cells may play an important role in the pathogenesis of the disease.