BACKGROUND: Among lots of drugs treating rheumatic arthritis, 99Tc methylenediphosphonate injection is a novel one whose anti-inflammation effect is enabled through electronic acquirement or deprivation of technetium.OBJECTIVE: To observe the effect of 99Tc methylenediphosphonate injection on rheumatic arthritis.DESIGN: Randomized controlled trial.SETTING: A Traditional Chinese Medicine Department of Xinqiao Hospital Affiliated to the Third Military Medical University of Chinese PLA.PARTICIPANTS: There were 85 patients diagnosed with rheumatoid arthritis and admitted to Xinqiao Hospital from March 2000 to December 2001. Sixty-six patients were enrolled in this trial for the manifestation of four or more of the following signs for 6 weeks: morning stiffness for at least one hour, more than 3 joints affected, symmetrical arthritis, positive rheumatoid nodules and serum rheumatoid factor, accelerated erythrocyte sedimentation and typical X-ray signs of rheumatic arthritis. Among the 66 patients, 23 were males and 43 were females with the age of 18-62 years old(mean 42.23 years) . The course varied from 2 months to 12 years. They were randomly assigned into treatment group and control group with 33 each.METHODS: The patients in the treatment group received 99Tc methylenediphosphonate injection injection and oral administration of placebo while those in the control group received placebo injection and oral administration of naproxen for 12 days. The morning stiffness duration, numbers of tender joints and swollen joints, erythrocyte sedimentation, C reactive protein, rheumatoid factor and total effective rate in the two groups were recorded and compared to assess the therapeutic effect.MAIN OUTCOME MEASURES: The morning stiffness duration, numbers of tender joints and swollen joints, erythrocyte sedimentation, C reactive protein and rheumatoid factor in the two groups and adverse effect of 99Tc met hylenediphosphonate injection.RESULTS: According to intention to deal with the analysis, none of the 66 duration and the reduction in the number of tender and swollen joints in the treatment group were better than those in the control group[ (70.39 ± 40. 32) minute, (3.27±2.21), (3.20±2.38) vs(35.82±24.65) minute,erythrocyte sedimentation, C reactive protein and rheumatoid factor in the two significantly higher than that(64% ) in control group(X2 = 4. 89, P ＜ 0.05).CONCLUSION: 99Tc methylenediphosphonate injection is better than naproxen in ameliorating articular swelling and tenderness as well as morning stiffness of rheumatic arthritis while the effect of 99Tc methylenediphosphonate injection on erythrocyte sedimentation, C reactive protein and rheumatoid factor was similar to that of naproxen.Huang WQ. Effect of 99Tc methylenediphosphonate injection on morning stiffness duration and tender swollen index of patients with rheumatoid arthritis.

Objective To evaluate the influence of the placement of instant hand disinfectant in compliance and qualified rate of hand hygiene of clinicians.Methods Hand hygiene compliance of clinicians in departments of gyne-cology,obstetrics,and general surgery were observed after the placement of instant hand disinfectant at the door-way of wards,hand specimens were taken to check hand hygiene effectiveness.Results Before and after placement of instant hand disinfectant at the doorway of wards of above departments,hand hygiene behaviors of 300 person-times were observed.Hand hygiene compliance rate among clinicians in department of gynecology,obstetrics,and general surgery increased from 32.33% to 74.33%,33.67% to 85.67%,and 26.33% to 64.00%,respectively(all P <0.001).Each department selected 98 hand hygiene specimens,the qualified rate was 98.98%,100.00% and 94.90% respectively.Conclusion The placement of instant hand disinfectant at the doorway of each ward can effec-tively improve clinicians’hand hygiene compliance and hand hygiene effectiveness.

Objective To evaluate the clinical outcome of the method of repairing donor site of foot after improved toe-to-thumb reconstruction utilizing superficial circumflex iliac artery perforator (SCIAP) chimeric flap.Methods Fourteen cases of thumb defect were recruited from April,2012 to January,2016.According to Gu Yudong's classification,5 cases met the criterion of type Ⅰ,4 cases met the criterion of type Ⅱ,and 5 cases met the criterion of type Ⅲ.For type Ⅰ,the thumb was reconstructed with the great toe wrap-around flap.For type Ⅱ and Ⅲ,the thumb was reconstructed by the combined tissue with mutual artery (great toe wrap-around flap,and the bonetendon tissue of the second toe).All the donor sites of foot were repaired utilizing SCIAP chimeric flap.Results All the reconstructed thumbs survived.Among 14 free flaps of donor site,1 case suffered venous crisis and survived after exploration and rescue surgery.Dorsal skin necrosis of the second toe was found in 1 case,which was healed by local skin flap transposition.All patients were followed-up ranged from 3 to 30 months (averaged at 16 months).In spite of slightly bloated,the color and texture of all the flaps' was satisfied,and the average healing time of the bone in the donor sites was 2.5 months.All patients did not feel painful and had no adverse effect when walking and running.Three months after the operation,5 slightly bloated flaps in the donor sites under went flap plastic and achieved better appearance.On the part of iliaca,there was only one inconspicuous linear scar without any discomfort.Conclusion Repairing donor site of foot after improved toe-to-thumb reconstruction utilizing SCIAP chimeric flap was an ideal method.Using this method,the reconstructed thumb can achieve good appearance and function,all the toes of donor site were reserved,and the disability of the donor site is minimized.

BACKGROUND:Neuroblastoma is a common solid tumor in children. Pediatric tumors are little affected by environmental factors, but closely related to child development. The suspension method is an effective and reliable method to harvest neoplastic stem cel s.
OBJECTIVE:To culture the cel clones of human neuroblastoma cel line SK-N-SH and to assess the biological properties of the cel clones.
METHODS:Using the suspension method with no serum media, tumor cel clones were obtained. Immunofluorescence method was used to identify whether tumor cel clones exhibit stem cel properties. SK-N-SH neuroblastoma was labeled by luciferase, and tumor cel clones and tumor cel s were seeded onto the back of nude mice to monitor cel proliferative properties in nude mice using in vivo imaging.
RESULTS AND CONCLUSION:Using the suspension culture method, SK-N-SH neuroblastoma cel s could successful y develop into cloning bal s. Under serum-free culture, cloning bal s were immunofluorescently used to detect molecular markers that showed strong positive expression. Cloning bal s subcutaneously implanted into
nude mice showed the strong ability of self-renewal and differentiation as stem cel s. The cel clones cultured by the suspension method strongly expressed Nestin, but weakly expressed glial fibril ary acidic protein, neuron-specific tubulin, possessing stem cel characteristics and strong proliferation and metastasis in nude mice.

Objective To investigate the influence of Addie injection combined with R -CHOP chemotherapy on immune function and quality of life in patients with non -Hodgkin's lymphoma.Methods A total of 76 patients with non-Hodgkin's lymphoma in the People's Hospital of Wenzhou from June 2015 to March 2017 were selected and randomly divided into the control group and the study group according to the digital table , with 38 cases in each group.The control group was treated with R -CHOP chemotherapy ,and the study group was treated with R -CHOP chemotherapy combined with Addie injection .After treatment,the clinical efficacy,toxic and side effects,immune function indicators ( CD3+,CD4+,CD8+,CD4+/CD8+) and quality of life ( KPS) score of the two groups were statistically analyzed.Results The total effective rate of the study group was 86.84％,which was higher than 65.79％ of the control group (χ2 =4.659,P<0.05).The incidence rates of diarrhea (18.42％),vomit and nausea(21.05％),bone marrow inhibition ( 23 .68％) and leukocyte reduction ( 21 .05％) in the study group were lower than those in the control group(42.11％,47.37％,47.37％,42.11％,χ2 =5.050,5.846,4.653,3.897,all P<0.05).There were no statistically significant differences between the two groups in CD 3+, CD4+, CD8+, CD4+/CD8+before treatment ( all P>0.05).After treatment,the CD3+,CD4+and CD4+/CD8+in the study group were higher than those in the control group,and the CD8+in the study group was lower than that in the control group , the differences were statistically significant(t=5.436,8.857,2.515,5.314,all P<0.05).After treatment,the scores of KPS of the two groups were higher than those before treatment(all P<0.05),and the KPS score of the study group was higher than that of the control group[(78.61 ±4.24)points vs.(70.04 ±4.10)points,t=8.957,P<0.05].Conclusion Addie injection combined with R-CHOP chemotherapy in the treatment of patients with non -Hodgkin's lymphoma can effectively improve the immune function of the patients ,reduce the incidence of side effects ,and improve the effect of treatment and the quality of life .

OBJECTIVEIn order to find out the current situation of wild and cultivated resources of Glycyrrhiza uralensis, at the same time, the contents of glycyrrhizic acid and liquiritin were detected by HPLC.

METHODField investigation was carried out, and chemical composition of collected underground part was analyzed in laboratory.

RESULTThe natural distribution range of wild G. uralensis in China has no significant change. We can still find its distribution in the distribution areas recorded ten years ago, but the intensity of species group has greatly changed. Estimated according to the survey data obtained by quadrat reserves and distribution (i.e., reserves per unit area) or cultivation area, that the current wild reserves is less than 500,000 tons and the less than 150,000 tons for cultivated. The analysis results of glycyrrhizic acid and liquiritin contents in 99 wild samples of collected underground part show that the average contents of glycyrrhizic acid and liquiritin were respectively 34.8, 17.3 mg x g(-1), of which 61.6% samples can reach the standard (not less than 20, 10 mg x g(-1)) marked by "Chinese Pharmacopoeia" (2005 edition); The average glycyrrhizic acid and liquiritin contents of cultivated samples were respectively 28.5, 15.3 mg x g(-1), which were much lower than the wild samples.

CONCLUSIONWe should enhance efforts in protecting the wild resources, strive to improve the quality of cultivated herbs, vigorously develop high-quality G. uralensis cultivation industries and promote farmers income to resolve the resource crisis, which is the reasonable way to achieve continuable use of G. uralensis resources.

China ; Chromatography, High Pressure Liquid ; Conservation of Natural Resources ; Ecosystem ; Flavanones ; analysis ; Glucosides ; analysis ; Glycyrrhiza uralensis ; anatomy & histology ; chemistry ; growth & development ; Glycyrrhizic Acid ; analysis ; Plant Structures ; anatomy & histology ; chemistry ; growth & development ; Plants, Medicinal ; anatomy & histology ; chemistry ; growth & development

Alkaloids and lignans from the stems of Piper betle were studied. Compounds were isolated and purified by repeated silica gel, reverse phase silica gel, Sephadex LH-20 column chromatography and preparative thin layer chromatography. The structures were elucidated on the basis of spectral analysis. From the ethyl acetate soluble fractions of the 70% acetone extract, ten compounds were isolated and identified as piperine (1), pellitorine (2), N-isobutyl-2E,4E-dodecadienamide (3), dehydropipernonaline (4), piperdardine (5), piperolein-B (6), guineensine (7), (2E,4E)-N-isobutyl-7-(3',4'-methylenedioxyphenyl)-2,4-heptadienamide (8), syringaresinol-O-beta-D-glucopyranoside (9),pinoresinol (10). All Compounds were isolated from the plant for the first time, and compounds 9 and 10 were isolated firstly from the genus.
Alkaloids ; analysis ; isolation & purification ; Chromatography, Thin Layer ; Lignans ; analysis ; isolation & purification ; Piper betle ; chemistry ; Plant Stems ; chemistry

At present the shortage of the resources of wild and rare medicinal plants is very serious in China. So grasping the situation and change tendency of medicinal plant resources comprehensive and real-timely, is vital significance to realize the sustainable using of medicinal plant resources. In order to use the remote sensing monitor technology and method to study the resources of the wild and rare medicinal plants, this article discusses the principle, method, technology and the principle and standard based on the operation and experiment of remote sensing monitor on the resources of wild rare medicinal plant.
China ; Conservation of Natural Resources ; methods ; Plants, Medicinal ; chemistry ; Satellite Communications

Objective To study the surgical techniques and the clinical curative ettect of the reconstruction of adjacent large finger pulps with the modified sensate free proximal ulnar artery perforator flap.Methods From February,2013 to May,2016,20 fingers in 10 patients with adjacent large finger pulp defects crossing the DIP joint were reconstructed by the modified senate free proximal ulnar artery perforator flap.All the pulps of every patient were recovered by 1 flap in the first stage and the artificial syndactyly of two digits was divided in 7 weeks after the flap transfer in the second stage.In order to reconstruct the sensation of two pulps in each patient,firstly both of the proximal and distal ends of the cutaneous nerve in flaps were dissected and anastomosed with the most lateral and medial palmar digital nerves of two adjacent fingers respectively,which were divided into 2 groups.Secondly the cutaneous nerve in the middle part of flap was cut and the two ends were anastomosed with the other two palmar digital nerves in the second surgery.There were 8 fingers in 4 patients with the index and middle finger pulp defects,12 fingers in 6 patients with the middle and ring finger defects.The flap size was from 5.0 cm×3.5 cm to 5.5 cm×4.0 cm,and the perforator artery was anastomosed with the palmar digital artery in 6 cases and with the joint branch of digital artery in 4 cases.Results Nine flaps survived uneventfully except the venous congestion was found in 1 flap which was salvaged successfully by acupuncture bleeding.Also,no congestion or ischemia of all the 20 pulp flaps occurred right after releasing the artificial syndactyly.The time of followed-up was from 11 months to 32 months with the average of 17 months after the second surgery.The static two point discrimination of the 2 groups of the sensate pulp flap was (7.3 ± 1.2) mm and (8.6 ±2.4) mm respectively.There was no significant difference between groups (P ＞ 0.05).These 20 pulp flaps were also assessed by the Semmes-Weinstein monofilament test with the result of diminished light touch in 14 pulps and diminished protective touch in 6 pulps.The total active motion in all 20 fingers was (248.0±4.5) °.No patients suffered cold intolerance and local pain but 1 had hypersensitiveness in the pulp flap.Conclusion The modified sensate free proximal ulnar arte~ perforator flap is a practical alternative for the reconstruction of two large adjacent pulps with satisfactory functional and aesthetic outcomes.

Objective The study compared the clinical outcomes among cleavage stage embryo transfer,two-step embryo transfer and blastocyst transfer in frozen-thawed cycles.To provide the clinical guidance for the patients with repeated implantation failure(RIF) to make a appropriate embryo transfer protocol.Methods In this retrospective study,a total of 252 patients with RIF were undergoing the frozenthawed embryo transfer (FET) treatment.They were divided into cleavage stage embryo transfer group (group A),two-step embryo transfer group (group B) and blastocyst transfer group (group C) according to the transfer protocols.Groups were compared including implantation rate,clinical pregnancy rate,live birth rate,miscarriage rate,multiple pregnancy rate and ectopic pregnancy rate.Results (1) There were no significant differences among three groups in age,infertility duration,infertility type,endometrial preparation protocols and embryo resuscitation rate.The rate of optimal embryo after resuscitation of group B was lower than that of group A (72.46％ vs 81.50％),showing significant differences (P'＜ 0.0125),but no significant difference were found between group B and C (72.46％ vs 77.42％),A and C (81.50％ vs 77.42％) (P'＞ 0.0125).(2) The implantation rate of embryos in group C was significantly higher than that in group A (38.46％ vs 21.61％) (P'＜0.0125),and there were no significant difference between group B and C (26.85％ vs 38.46％),A and B (21.61％ vs 26.85％).The clinical pregnancy rate of group B was significantly higher than that of group A (62.50％ vs 45.09％) (P'＜0.0125),and there were no significant differences between the others.No statistically differences existed in regard to the live birth rate among three groups,but the group B than the group A (50.00％ vs 35.71％),the group B than the group C (50.00％ vs 34.62％) showed an increasing trend.(3) No statistically differences existed in regard to abortion and multiple pregnancy rate among three groups (P ＞ 0.05).No case of ectopic pregnancy occurred in group B and group C,while in the group A,there were 7 cases of ectopic pregnancy.Conclusions For RIF patients,two-step embryo transfer and blastocyst transfer are relatively safe and effective transplants in frozen-thawed cycles compared to cleavage embryo transfer.