Objective To observe the morphological changes in alcohol-induced pregnanted golden hamster neural tube defects and eye abnormalities, and to study quantitatively the alterations of apoptosis during teratogenesis. Methods The pregnanted golden hamsters were killed 4,8,16,24, 48 and 72 hours after teratogenic dose alcohol exposure respectively, and both embryos and fetuses were obtained. The LM, SEM and stereomicroscope were used to observe the histological alterations during the teratogenesis of the neural tube and the eye. With the TUNEL and TEM methods, the apoptosis were studied qualitatively. Results 1^The closure of the golden hamster neural tube and the formation of the optic cup were significantly retardation in the experimental groups. 2^After 8h of maternal alcohol exposure, the apoptosis of neural epithelial cells increased apparently, especially in the prosencephalon and neural crest.At either 16h or 24h after treatment, the index of apoptosis was also higher than that in the control groups, and the apoptosis chiefly located in the neural crest and optic grooves.After 48h, this index gradually declined to the level of control groups.Conclusion Alcohol can delay the closure of the golden hamster neural tube and the formation of the optic cup. Meanwhile it can cause excessive apoptosis on these structures.These alterations are probably partially involved in the mechanism of alcohol-induced neural tube defects and eye abnormalities.

This paper discusses the problem of the periods of Medical Ethics. The writers suggest that Medical Ethics should be distingwished three periods: Medical Ethics, Biomedical Ethics and Bioethics.

Objective The androgen signaling pathway is involved in the regulation of early follicular growth and follicular atresia, and sex hormone binding globulin (SHBG) is an important factor in regulating the level of local ovarian androgen.This study was to investigate the effects of androgen on the expression of SHBG in ovarian granulosa cells.Methods Human ovarian granule cancer cells were cultured with 0 nmol/L dihydrotestosterone (DHT), 500 nmol/L DHT, or 500 nmol/L DHT + 60 μmol/L Flutamide.The expression of SHBG was detected by Western blot and immunofluorescence staining.Hyperandrogenism (HA) was induced in 6 SD rats by subcutaneous injection of dehydroepiandrosterone (DHEA) and another 6 rats were included in the vehicle control group.At 35 days after modeling, all the rats were sacrificed for measurement of the level of SHBG in the blood from the inferior caval vein by ELISA, the expression of SHBG in the ovarian granulosa cells by immunohistochemistry, and expressions of androgen receptor (AR) and SHBG in the liver by Western blot.Results At 24 hours after modeling, the expression of AR was significantly upregulated in the 300, 400, and 500 nmol/L DHT groups as compared with the 0 nmol/L DHT group (1.06±0.02, 1.61±0.11, and 2.38±0.14 vs 1.06±0.03, P<0.05), and so was that of SHBG, increasing in a concentration-dependent manner, most significantly at 500 nmol/L (P<0.01).Both the expressions of AR and SHBG proteins remarkably elevated in the 500 nmol/L DHT group in comparison with the 0 nmol/L DHT group (P<0.01), but markedly downregulated as compared with the 500 nmol/L DHT + 60 μmol/L Flutamide group (P<0.05).Immunofluorescence staining showed that DHT promoted while the addition of Flutamide inhibited the expressions of AR and SHBG.Immunohistochemical staining of the ovarian tissue revealed a high level of SHBG in the HA rats.Compared with the control group, the HA animals exhibited a significantly decreased expression of serum SHBG (4.80±0.35 vs 2.41±0.14, P<0.01) and that in the liver, but a markedly increased level of the AR protein (P<0.05).Conclusion Activation of the androgen signaling pathway by DHT can promote the expression of SHBG in rat ovarian granulosa cells.

Objective To establish a method for determination of emodin content in Shengfa granule.Methods HPLC was used.Mobile phase consisted of methanol-0.25 %phosphoric acid (80 ∶20) with UV detection at 254 nm,the analytical column was YMC-Pack ODS-A.Results The linear range of emodin was 0.021 ?g～0.155 ?g(r=0.9999)while the average recovery rate was 99.03 %(RSD=1.40 %,n=6).Conclusion The methods is suitable for determination of emodin content in Shengfa granule.

Firstly, the essay introduces the definition of bio-burden, the significance of determination of bio-burden and the specific performance requirements. Then, the essay introduces the method selection and validation on the bio-burden determination of surgical drapes, gowns and clean air suits.
Equipment Contamination ; Surgical Attire ; microbiology ; standards ; Surgical Drapes ; microbiology ; standards

Objective To discuss the effect of early combined therapy of sertraline and psychotherapy on the rehabilitation of the patients with post-stroke depression (PSD)and observe its effect on plasma and platelet serotonin(5-HT). Methods All of 130 patients with PSD were divided into sertraline treatment group (65 cases) and routine treatment group (65 eases). Every patient was given routine treatment according to treatment guideline of cerebrovascular disease after admission. The sertraline treatment group received twelve-week sertraline treatment and psychotherapy, while the routine treatment group only received psychotherapy. Patients were evaluated by HAmilton rating scale for depression (HRSD) and serf-rating depression scale (SDS) before treatment and at the ends of the 4th and 8th week treatment, respectively. Meanwhile, the levels of plasma and playlet 5-HT of every patient were measured by the enzyme immunoassay methods. Results The scores of HRSD and SDS were lower in sertraline treatment group than those in routine tretment group at the ends of the 4th and 8th week. The rate of decrease of HRSD and SDS in sertraline treatment group was higher than that in routine treatment group at the end of the 8th week (Pall<0.01).The levels of platelet 5-HT in sertraline treatment group was lower at the ends of the 4th and 8th week than those before, treatment (P< 0.01).No significant change in plasma 5- HT level could be observed before and after treatment in two groups(P> 0.05). Conclusions Early treatment by sertraline can release the symptom of PSD and promote rehabilitation of PSD patients. The sertraline treatment can reduce the level of platelet 5- HT in PSD patients. However, the effect of plasma 5-HT value is not clear.

Objective To find out the microalgae species in the spring water and the pollution route in the production process of drinking spring water. Methods 42 samples were collected from the workshop, the spring source, the product line and the repeated barrels in a water plant. The microalgae were examined by microscope, CCD imaging system and scanning electron microscope. Results 18 microalgae species were detected from the 42 samples including 4 species in Cyanophyta, 9 in Chlorophyta, 4 in Bacillariophyta and 1 in Euglenophyta. The dominant species occurred in samples of the floor around the workshop and the floor beside the wellspring bump were Achnanthes biasolettiana and Cymbella affinis, in the spring source were Chlamydomonas sp. and Ulothrix tenerrima, in the spring water barrel was Scenedesmus obliquus. Chlorella vulgaris was detected in all processes before the super-filter,and after filtered, no microalgae was detected, but in the product water, Chlorella vulgaris was still detected after enriched culture. Conclusion Usually, the microalgae polluted drinking spring water products is mainly Chlorella vulgaris and the pollution route is reusing of barrels.

Objective To explore the curative effect and indication in coverage of the thumb soft tissue defect by five kinds of minute flaps. Methods From January 1998 to March 2003, 52 cases with skin and soft tissue defect of the thumb were treated by five different kinds of flaps: 5 underwent the wrapping flap with nail from the big toe, 15 with the reversible thumb dorso-ulnar artery island flap, 10 with the thenar flap, 8 with the lateral digital island flap, 14 with the index finger dorsal island flap. There were 40 males and 12 females, aged from 17 to 35 years (average, 25 years). The maximum area of the repair flap was 5.0 cm?2.5 cm, and the minimum area was 2.0 cm?1.5 cm. Results All flaps survived. After 6 to 36 months postoperative follow-up, with an average of 20 months. The blood supply and texture of the flaps were excellent, their outer aspect and function were satisfactory. Among 46 cases of flaps with their proper or connecting nerves, the average distance of the two-point discrimination was 5.8 mm in the wrapping flap with nail from the big toe, 8.5 mm in the reversible thumb dorso-ulnar artery island flap, 7.0 mm in the thenar flap, 6.2 mm in the lateral digital island flap, 8.8 mm in the index finger dorsal island flap. Of the 6 cases that the nerves were not connected, the healed flaps had only the pain and touch sensation, the two-point discrimination was poorly recovered. Conclusion The five kinds of flaps are safe and reliable for repairing thumb soft tissue defect, among which the wrapping flap with nail from the big toe and the lateral digital island flap have the advantage of the best sensation recovery, the reversible thumb dorso-ulnar artery island flap and the thenar flap are easy to perform with minimum damage. Better curative effect can be obtained by choosing different flaps according to the site of tissue defect.

Objective To study the effect of tamoxifen on the proliferation and apoptosis of germ cells and to evaluate its clinical application to idiopathic oligospermia. Methods 41 oligospermia patients were treated with tamoxifen.Testicular tissues obtained by Tur Cut biopsy needle,both before and after the treatment,were observed under electron microscopy,the incidence of apoptosis and PCNA expression in germ cells being detected. Results Testicular pathological changes in different degree were observed on electron microscopy.Compared with the normal controls.PCNA proliferation index (PI) was lower ( P

Objective To study the ultrastructural changes in three different zones of benign prostatic hyperplasia(BPH). Methods The ultrastructural changes in peripheral,transitional and central zones(PZ,TZ and CZ)of prostates from 10 patients with BPH were observed with transmission electron microscopy. Results Glandular epithelia layers in TZ of BPH were increased.The stroma in TZ was mainly composed of smooth muscle cells (SMC) of vigorous proliferation and fibroblasts.Mast cell and lymphocyte with cytoplasma membrane merge were observed in this zone.In PZ and CZ of BPH, SMC showing degenerative changes,such as,nuclear pyknosis,dilatation of perinuclear space and vacuolation of cytoplasma were frequently seen. Conclusions There are significant differences in the ultrastructure of three zones in BPH.