Objective To investigate the correlation between urine protein and VEGF in of IgA nephropathy patients .Methods Selected data of 67 patients of IgA nephropathy patients in Xinxiang Medical University ,VEGF expression was tested by immuno-histochemistry in renal tissue ,enzyme linked immunosorbent assay were used to detect the serum and urine levels of VEGF and ana-lyze the distribution of different pathological parameters .Analyzed correlation between urine protein and VEGF by regression analy-sis ,and analyzed the therapeutic effect and biochemical index in different combination of urinary protein and VEGF .Results (1)u-rine and serum VEGF levels in patients with IgA nephropathy were higher than healthy controls and patients with lupus nephritis group ,expression of VEGF in renal tissue was higher than that of lupus nephritis group (P<0 .01) ,and the difference of VEGF lev-el of IgA nephropathy group in Lee's classification ,renal tubular interstitial lesions and Katafuchi total score had statistically signifi-cant(P<0 .05) ,and increased with the severity of the pathological ;(2)Renal tissue VEGF urine and blood VEGF level were statis-tically significant in different levels of urinary protein of IgA nephropathy ,and increased according to urine protein was less than 1 .0 g ,1 .0 to 3 .0 g and more than 3 .0 g(P<0 .05) .Levels of urinary protein and VEGF expression in the kidney ,urinary VEGF and serum VEGF levels were positively correlated (r=0 .59 ,P<0 .01 ;r=0 .46 ,P<0 .01;r=0 .37 ,P<0 .05);(3) IgA nephropathy with urinary protein level between different levels of VEGF sAlb ,blood IgA ,Cr ,eGFR ,β2-MG ,urine osmotic pressure ,BUN and the effect rate of treatment differences were statistically significant (P<0 .05) .Conclusion Renal tissue VEGF expression in pa-tients with IgA nephropathy enhance ,while levels of VEGF in blood and urine were increased and associated with urinary protein . Pathological factors and treatment effect have statistically significant difference between different urinary protein and VEGF combi-nation .

Objective To prepare the paliperidone long-acting suspension for injection and study the quality of it.Methods The optimum formula was measured by using orthogonal design method regarding the content of paliperidone, Na-CMC,mannitol and the ground time as variable factors. Orthogonal design table L9 ( 34 ) was used in the experiment. The paliperidone long-acting suspension for injection was prepared by grinding the dispersion,and its quality was evaluated. Results The optimum prescription was as follows: using paliperidone 40 g, Na-CMC 15 g, mannitol 60 g, purified water 1 000 mL, appropriate amount of NaH2 PO4 and NaOH, and grinding for 1 h. Paliperidone long-acting suspension for injection was characterized by an average pH of 7. 0,and ratio of average sedimentation volume of 90. 3%. The suspension released smoothly within 1 h in vitro and achieved sustained release. The suspension was stable in a freeze-dried powder. Conclusion The preparation for paliperidone long-acting suspension for injection is simple and stable,which may be a novel drug delivery system.

Objective To explore the presence of biliverdin reductase (BR) activity in adult worms of Schistosoma japonicum in vitro. Methods The soluble fraction was isolated from homogenates of adult worms of S. japonicum, and incubated with biliverdin under different pH conditions and buffers. The time dependency of this enzymatic reaction was also detected. ResultsThe soluble fraction of the homogenates of adult worms could degrade biliverdin in vitro, the BR activity was 43.30 nmol/(mg?min), with its optimal condition at pH 8.7. The rate of reaction peaked at 15 min of incubation for the BR activity. Conclusion The presence of BR activity in adult worms of S. japonicum was firstly demonstrated.

The clinical data of 96 intubated inpatients with restricted cervical movement from June 2012 to June 2014 were retrospectively reviewed.They were divided into fiberoptic bronchoscope, Shikani optical stylet and McCoy laryngoscope groups (n =32 each).Identical anesthesia induction was applied.Compared with McCoy laryngoscope group, the total success rate and one-time success rate of tracheal intubation was higher in Shikani optical stylet group, tracheal intubation time was shorter and postoperative complications were fewer (P ＜ 0.05).Compared with group F, endotracheal intubation time was shorter (P ＜0.05).However, heart rate, mean arterial pressure (MAP), oxygen saturation (SpO2), total success rate of tracheal intubation, one-time success rate of tracheal intubation and complication rate showed no significant inter-group differences at all timepoints (P ＞ 0.05).Shikani optical stylet intubation has a less impact on hemodynamics and intubation time is short so that it is ideal for surgical patients with restricted cervical spine.

Objective To explore the effect of ginseng combined with transplantation of bone marrow mesenchymal stem cells(BMSCs)on functional recovery of rats with spinal cord injury(SCI). Methods Forty-eight female Wistar rats were randomly assigned to following four groups(n=12 per group):the sham operation group was treated by opening the vertebral lamina and exposing spinal cord without SCI;the SCI model group was reproduced by using improved Allen bump method and afterwards no treatment was given;methyl prednisolone(MP)group was treated by MP pulse treatment after SCI,including intravenous injection of MP 30 mg/kg immediately after SCI and 4 hours later the same injection was repeated,and then the same intravenous injection 2 times daily,3 days in total;The ginseng+BMSCs group was treated by orally taking ginseng ultra-microgranules 300 mg/kg after SCI,twice a day for 20 days and BMSCs 5μL(concentration 1×107 cell/μL)transplantation was carried out in SCI region on the 7th day after SCI. In the above 4 groups,the ethological observation(BBB scores)was done regularly and on the 30th day after operation,silver staining was applied to investigate the changes of spinal cord,and neuro-electrophysiological tests including somatosensory evoked potential(SEP)and motor evoked potential(MEP)were performed. Results In sham operation group,after surgery the movement of both hind limbs became temporarily sluggish and on the 7th day their functions recovered to approximately normal. In SCI model group,after injury paralysis of both hind limbs occurred,while in the MP group and ginseng+BMSCs group,different degrees of functional recovery of the injured limbs developed,and the recovery in ginseng + BMSCs group was more significant. Compared with sham operation group,after surgery the BBB score was reduced markedly at various time points in SCI model group;compared to the SCI model group,the BBB scores in MP and ginseng+BMSCs groups were increased significantly,especially more remarkable in ginseng+BMSCs group(all P<0.05),and beginning from the 12th day after operation,the difference became obvious (5.23±1.22 vs. 3.61±1.03, P<0.05). Histological detection showed that in sham operation group,the structure of spinal cord was complete,neurons distributed evenly in the gray matter and a large number of silver staining positive nerve fibers paralleled to each other and arranged regularly;in SCI model group,fragmented construction was present and the defects of gray and white matters were prominent. Compared with the SCI model group, the extents of tissue necrosis in MP and ginseng + BMSCs groups were ameliorated. The neuro-electrophysiological tests demonstrated that in SCI model group,the loss of normal wave form occurred. Compared with SCI model group,in MP and ginseng+BMSCs groups,after treatment latent periods(ms)were shortened prominently in different degrees of SEP and MEP,and their peak-to-peak values(mV)were increased obviously;the improvement in potential in ginseng+BMSCs group was greater than that in MP group〔SEP:latent period(ms):3.31±0.36 vs. 4.66±0.33, peak-to-peak value(mV):0.10±0.01 vs. 0.05±0.01,MEP:latent period(ms):3.40±0.13 vs. 4.24±0.31, peak-to-peak value(mV):41.12±0.56 vs. 16.46±2.83,all P<0.05〕. Conclusion A combined treatment of ginseng and BMSCs transplantation can effectively promote the recovery of neural function for rats with SCI.

Objective To explore the activity of heme oxygenase and immunolocate the enzyme in the adult worms of Schistosoma japonicum .Methods Microsomal protein was isolated from the homogenate of adult S\^japonicum , heme degradation and effect of different pH conditions and buffers on degrading reaction were investigated by incubating microsomal protein with hemin. The slices of whole worm and cells of S\^japonicum were prepared, distribution of HO in schistosome was studied by immunofluorescent and alkaline phosphatase(AP) \|immunocytochemical assays.Results Microsomal protein of adult worms can degrade the heme in vitro , the activity being 56\^7 nmol bilirubin/(mg?min).The optimal pH was 8\^7. Immunofluorescent and AP\|immunocytochemical assays revealed that the HO distributed dispersively in the worm, and located in cytoplasm.Conclusion The presence of HO was firstly proved in S\^japonicum .

Objective To detect the infection of Schistosoma japonicum in mice with a novel test based on agglutination of hybridoma cells and to study the mechanism of the hybridoma cells agglutination. Methods The procedure was developed with a murine cell line H226 producing a monoclonal antibody specific to schistosome 31/32 kDa antigen and sera collected from mice infected with different numbers (10,30,50) of S. japonicum cercariae in different period. Immunofluorescent test was carried out with the hybridoma cells and schistosome-infected sera. Results The circulating antigen was detected by the test as early as 2 weeks after a heavy infection and all mice showed positive results in the test by 5 weeks after infection. The titers of antigen rose along with the lime post infection, and the tilers of sera from heavy infection were statistically higher than that from the mice receiving a lower number of cercariae. Specific yellowish green fluorescence appeared on the membrane of the hybridoma cells; no signal was detected inside. Conclusion Hybridoma cell agglutination test (HCAT) may become useful to diagnose schistosomiasis.

Objective To explore the feasibility, difficulties and indications of video-assisted laparoscopic pancreaticoduodenectomy. Methods Clinical records of 5 cases of laparoscopic pancreaticoduodenectomy in this hospital from November 22, 2002 to April 28, 2005 were analyzed, with respect to preoperative diagnosis, surgical methods and procedures, intraoperative blood loss, operation time, difficulties encountered and countermeasures, and postoperative recovery. Related parameters included patient's temperature, drainage volume, blood routine, liver functions, amylopsin changes, postoperative complications, bowel movement recovery, and length of hospital stay. Results All the 5 cases of operation were successfully performed, with an operation time of 360~660 min (mean, 528 min) and an intraoperative blood loss of 150~2 000 ml (mean, 770 ml). Pathological examinations revealed 3 cases of well-differentiated papillary adenocarcinoma of the duodenum, 1 case of endocrine small-cell carcinoma of the head of pancreas, and 1 case of papillary adenomatoid hyperplasia of the duodenum with malignant changes accompanying intermediated-differentiated adenocarcinoma of upper and middle intrahepatic biliary duct. Postoperatively, a small amount of pancreatic leakage occurred in case number 1 and a digestive tract stress ulcer happened in case number 2, all of which were cured by symptomatic management. In case number 4, a recurrent pancreatitis with pulmonary infection was observed, and the patient died from stress ulcer hemorrhage on the 39th postoperative day. Except for the case number 4 in which the patient's highest temperature reached 39.5 ℃ after the recurrence of pancreatitis, the highest temperatures of the other 4 patients were all

Objective To explore the value of MRI features of the appendixes bone in differentiating vertebral myeloma from metastatic tumor. Methods There were vertebral myelomas and metastatic tumors in 32 cases respectively involved the appendixes bones,the number,positions,forms,size,distribution ranger and T_1 of the lesions,the vertebral pedicles and the diameters of the cross sections of vertebrallaminas were analysed.Results Among the 32 cases of vertebral myeloma,the destruction of appendixe was bilateral in 21 cases andunilateral in 11 cases.Totally 89 lesions were checked out ,of them,the lesions were the form of mass or nodule in 39,patch in 28,mixed form in 12.In compared with its adjacent and normal one,the ratio of evary invaded vertebral pedide , vertebral lamina and spinous process was between 1.048~2.135 . There was significant difference (P0.05).Conclusion The difference of MRI features of the appendixes bone invaded by vertebral myeloma and by metastatic tumor is helpful in distinguishing vertebral myeloma from metastatic tumor.

Objective To investigate the characteristics of interactive effects of magnetic field of two intensities combined with high-temperature and noise simulating ship environment. Methods Orthogonal trial design of three factors and two levels and variance analysis method were employed. The rabbits and rats were grouped into eight combined exposure groups and eight controls, respectively, according to orthogonal table L_8 (2~7). Animals in all groups underwent the experiments and the indices were determined at the same time. Results Analysis of the radius of capillary vessels on rabbit's conjunctiva and HSP70 of rat's liver and spleen showed that magnetic field was the dominant factor (P