A hundred thirteen ears in 76 cases underwest the traumatic deafness caused by beating at the ear and head were examined with the Evoked Potential System of Nicolet Spirit and the Pure tone audiometry.113 ears were divided into two groups. One group consisted of 32 ears with the tympanal perforation, which were further divided into small, medium and big perforations. The second group consisted of 81 ears without tympanal perforation,which were classified into mild, moderate,serious,severe and profound deafness. The two groups were all examined by EcochG and ABR. The wave latent periods of EcochG and ABR of two groups (small, medium perforation;mild,moderate deafness)were statistically analysed and were compared with those of the normal control group. The results of the square deviation analysis and Q test showed the significant difference (76. 7%, P

OBJECTIVE To investigate the effect of norepinephrine (NE) on expression of interleu?kin-6 (IL-6) in human macrophages and explore its pro-inflammatory and pro-atherosclerotic mecha?nisms. METHODS Murine U937 macrophages were cultured and stimulated with 0.01-10μmol·L-1 of NE for 6, 9, 12, 24 and 48 h. The IL-6 mRNA level of 24 h was analyzed by RT-PCR, and IL-6 protein expression in the supernatant at 0, 6, 9, 12, 24 and 48 h was detected by ELISA. After 24 h, intracellular reactive oxygen species (ROS) generation was observed by DCF fluorescence. The cells were pretreated with antioxidant N-acetylcysteine (NAC), complexⅡinhibitor thenoyltrifluoroacetone (TIFA) and NADPH oxidase inhibitor diphenyleneiodonium (DPI) for 1 h, and stimulated with different concentrations of NE for 24 h, before the level of IL-6 protein was detected by ELISA. RESULTS The expression of IL-6 mRNA and protein increased with the concentration NE 0.01-10 μmol · L-1 and incubation time. IL-6 mRNA and protein levels in macrophages were 2.62 and 4.47-fold those in cell control group when treated with NE 1.0μmol·L-1 for 24 h. Meanwhile, as the concentration of NE increased, the generation of ROS was 1.87, 2.56, 2.91 and 5.36-fold that of cell control group (P<0.01). NAC 10 mmol · L-1 and DPI 10 μmol · L-1 significantly antagonized the effect of NE on IL-6 expression, but TIFA had no effect. CONCLUSION NE upregulates IL-6 expression, which may contribute to the formation and develop?ment of atherosclerosis via ROS mediated by NADPH oxidase in macrophages.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Combined Modality Therapy ; Female ; Humans ; Middle Aged ; Moxibustion ; Needles ; Postoperative Complications ; etiology ; physiopathology ; therapy ; Scalp ; Treatment Outcome ; Urinary Retention ; etiology ; physiopathology ; therapy ; Urination ; Uterine Cervical Neoplasms ; surgery

OBJECTIVE:To get to know the revising tropesis of partial Chinese traditional medicine in Edition2005Pharmacopoeia of PPC so as to provide suggestions for the revising work.METHODS:Revising tropesis about partial new kinds and new items in Edition2005Pharmacopoeia of PRC(Draft showed in public)were analyzed in detail.RESULTS:Edition2005Pharmacopoeia of PRC adopted some new and mature skills besides the traditional method of identifying just from experience,i.e.from the appearances and quality of medicine.CONCLUSIONS:The standard of quality criteria in new edition pharma?copoeia is tend to be more rationalizing and standardizing,thus more close to the international quality criteria of natural medicinal herbs.

To investigate the effects of electro-acupuncture at Shuigou (DU26) on latency and amplitude of motor evoked potential (MEP) in rats after cerebral infarction.

Objective To compare the hemodynamics features of recurrent hepatocellular carcinoma between contrast-enhanced ultrasonography (CEUS) and contrast-enhanced MRI (CEMRI).Methods Forty three recurrent hepatocellular carcinoma lesions in 39 patients were evaluated by CEUS and CEMRI.All lesions were proved by operation or needle biopsy.The dynamic enhancement and washout process on CEUS and CEMRI were analyzed.Results On both two methods,all lesions showed hyperenhancement.Among which,30.2％ (13/43) lesions showed hyperenhancement during arterial phase and no washout was found during portal venous phase,37.2 ％ (16/43) lesions hyperenhanced during arterial phase and washout during portal venous phase.During late phase,the enhancement of 60.5％ (26/43) lesions decreased and 7.0％ (3/43) lesions showed no washout on CEUS or CEMRI.The overall concordance ratio between two methods was 67.4％ (29/43).Difference of hemodynamics features between CEUS and CEMRI was not statistically significant.Conclusions CEUS can accurately display the hemodynamic features of recurrent hepatocellular carcinoma,and enjoy high consistence with CEMRI.

Objective To screen and identify the phage-display random 7 amino acid peptide specific to the systemic lupus erythematosus(SLE) and analyze its practical significance. Methods Using the phage random 7 peptide library screening, the SLE specific phage clones are obtained after binding with the mixture of sera from 30 SLE patients and 30 normal controls as ligand respectively. Then the Dot-ELISA is used to identify the SLE specific phage clones reactive to sera of the SLE patients and normal controls individually. Finally the identified phage-display random 7 amino acid peptides are sequenced and it's homology with the antigenic epitope of human being and other are also analyzed. Results Total 12 of the phage-display random 7 amino acid peptide are obtained by phage peptide library screening and the Dot-ELISA identification. Sequence analysis shows that the identified phage-display random 7 amino acid peptide epitope have homology with E. coli, Salmonella and human immunodeficiency virus, but not with that of human being. Conclusion SLE-specific peptides screened by phage random peptide library maybe used to diagnosis the SLE. Meanwhile, the antibodies in SLE patients which are combined with the Pathogen epitope, suggest that SLE maybe relate to pathogen infection.

Objective:To observe the clinical efficacy of combining electroacupuncture with long needle sacral nerve acupuncture in the treatment of diabetic overactive bladder.Methods:A total of 90 patients with diabetic overactive bladder were randomly divided into an observation group and a control group, each of 45. In addition to the basic treatment for diabetes, the observation group received electroacupuncture combined with long needle sacral nerve acupuncture 5 times a week for 4 weeks, while the control group was given 5mg of oral solinax succinate once a day as a course of treatment. One week before the beginning of the experiment and one week before its end, urination diary cards were used to record the average number of times of daytime and nighttime urination daily, as well as any urgent urination and urinary incontinence during the week. Bladder overactivity syndrome score (OABSS), the bladder overactivity quality of life questionnaire (OAB-q), maximum urine flow rate (Qmax) and mean urine flow rate (Qave) were employed to quantify urination status, life quality and the urodynamics of the 2 groups before and after the treatment.Results:After the treatment, significant improvement was observed in the average weekly incidence of daytime and nighttime urination, of urgent urination and of urinary incontinence. The average OABSS, OAB-q, Qmax and Qave scores improved in both groups, but the experimental group showed significantly better improvement than the control group.Conclusion:Electroacupuncture combined with sacral nerve needling can significantly reduce the frequency of urination, relieve the symptoms of urgent urination and incontinence, and improve the rate of urine flow, improving the life quality of patients with diabetic overactive bladder.

Objective To explore the effect of bee-needle therapy on treatment of patients with rheumatoid arthritis.Methods Seventy five patients with rheumatoid arthritis were randomly divided into bee-needle group,drug group and bee-needle plus drug group.Therapeutic effect was compared.Results After treatment and follow up,there were significant differences in degree of joint pain,the number of swollen joints,tender joint count,morning stiffness time,the number of pain joints,HAQ,ESR,RF,and CRP among three groups (P ＜ 0.05).Conclusion Bee-needle therapy can significantly relieve the clinical symptoms of patients with rheumatoid arthritis.

Objective To explore the effect of bee-needle therapy on treatment of patients with rheumatoid arthritis.Methods Seventy five patients with rheumatoid arthritis were randomly divided into bee-needle group,drug group and bee-needle plus drug group.Therapeutic effect was compared.Results After treatment and follow up,there were significant differences in degree of joint pain,the number of swollen joints,tender joint count,morning stiffness time,the number of pain joints,HAQ,ESR,RF,and CRP among three groups (P ＜ 0.05).Conclusion Bee-needle therapy can significantly relieve the clinical symptoms of patients with rheumatoid arthritis.