Objective Dentritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) is an important receptor for pathogenic microbes, and it acts as pathogen-recognition receptor (PAMP) and provides a bridge between the innate immunity and acquired immunity. It is meaningful to clarify whether DC-SIGN takes part in the course of cutaneous Trichosporon asahii infection and how it manages the other cytokines. Methods Twenty-five male mice were hypodermically injected with 3.2?107 CFU/ml of Trichosporon asahii suspension on one side of the back, the other side of the back received no injection to serve as control. Another five mice were treated with 0.9% saline solution as control. The cutaneous specimens were obtained on the 1st, 3rd, 7th and 14th day after inoculation. The expression of DC-SIGN gene and the other genes related to cutaneous immunity after cutaneous infection of Trichosporon asahii were investigated by RT-PCR and microarray. Results The DC-SIGN products containing 242 bp of nucleic acid from the skin specimens of the inoculated sites were amplified with RT-PCR on the 3rd, 7th and 14th day after inoculation. The results of microarray showed that a series of genes were down-regulated, including complement component 2, TNF-inducible protein cg12-1 (CG12-1), interferon-induced protein with tetratricopeptide repeats 3 (Ifit3), CD53 antigen, CD22 antigen, and prostaglandin E receptor 4. Conclusions The results suggest that DC-SIGN takes part in the course of Trichosporon asahii infection. DC-SIGN not only inhibits Th1 type of cell-mediated immunity, but also inhibits some of complement factors, prostaglandin E receptor and immunoglobulin binding protein 1b, thus resulting in chronic and intractable infection of Trichosporon asahii. The detailed roles of DC-SIGN in the course need to be further studied.

Objective:To investigate the clinicopathological factors affecting the prognosis of colorectal cancer patients with liver metastasis and how to select therapeutic method. Methods:The clinical records of 146 colorectal cancer patients with liver metastases who were admitted in Cancer Hospital of Guizhou Province from March 1997 to March 2007 were collected and made a retrospective analysis. The survival rate of the 146 patients was calculated by using life table method. Kaplan-Meier method and log-rank test were used for univariate analysis on clinicopathological features and therapeutic modalities. The multivariate analysis was performed by using COX regression model. The prognostic index (PI) of patients was calculated based on the result of multivariate analysis. The patients were classified into different risk groups according to PI value and the survival rate was compared between the different groups. Rusults:The overall 1-, 3-, and 5-year survival rates were 62.0%, 15.5%and 6.2%, respectively. Univariate analysis revealed that the following factors were related with the prognosis of colorectal cancer patients with liver metastasis. They included pathological classification, histological grade, serum CEA(carcinoembryonic antigen)level, primary tumor resection, local lymph node metastasis, number and size of liver metastases, distribution and initiation time of liver metastases, extrahepatic invasion, with or without surgery and che-motherapy for liver metastasis, and chemotherapeutic regimen selection. Multivariate analysis showed that the serum CEA level, extrahepatic metastasis, number and size of liver metastases, primary tumor resection, and chemotherapeutic regimen were independent prognostic factors for colorectal patients with liver metastasis.Conclusion:The therapeutic modality had an obvious effect on the prognosis of colorectal cancer with liver metastasis. Active treatment for primary tumor and metastatic lesions increased the survival rate of patients. Serum CEA levels, with or without extrahepatic metastases, and the number and size of liver metastases were prognostic factors. PI value could be used to predict the prognosis of colorectal cancer patients with liver metastasis.

Objective To report the first case of disseminated trichosporonosis in China.Methods A series of clinical,histopatholog ic,and mycologic studies were carried out in a 20year-old-female patien t,who had no definite underlying disease.The causative fungus was identified according to culture,biochemical t est and DNA sequencing.Results Abundant fungal spores,yeasts and h yphae were shown in the specimens tak en from infectious granulomas.The fungal c olonies in the culture of skin lesion s or crusts,liver,oral psedomembra ne,vagina,nasal cavity,urine,and sto ol were the same.The colonies exhibi ted creamy white to yellowish in colo r,rugose on the surface,and hyphae at t he periphery.Under microscopy,there were a lot of rectangular arthrospores,round or oval spores w ith or without buddings,as well as br anched and septate filaments.The isolated fungus was unanimously identified as Trichosporon asahii(No.AS 2.2174)through culture,bio-chemistry and molecular biology tests.The patient's condition was impr oved obviously after the combinatio n treatment of amphotericin B liposom e(Amphotec)and fluconazole.Conclusion The extensive and lasting systemic impairments caused by T.asahii have scarcely been reported at home a nd abroad.Combination therapy with amphotericin B and fluconazole is effective.

Objective To report Trichosporon asahii first isolation and identification of TH chosporon asahii in chain.Methods A clinical infection fungus strain was isolaled from mouth pseudomembrane vaginal,nasal secretions,urine,faces,and live puncture tissue,injured skin tissue of a female patient,who had no clear underlying disease.Through cultured in medium,biochemical assay,identification of the bacterial race molecular biology test and DNA sequencing.Result We demostrated that the fungus strain was unanimously identified as Trichosporon asahii.Conclution The Trichosporon asahii could induce the disseminated Trichosporonosis.

OBJECTIVE: To develop a high performance liquid chromatography method for the determination of imatinib in rat plasma and study its pharmacokinetics. METHODS: Plasma was deposited by perchloric acid. The analytical column was ZORBAX SB-C18 (4.6 mm × 150 mm, 5 μm). The mobile phase was acetonitrile-water-0.1% trifluoroacetic acid (19:61:20) and the flow rate was 1.0 mL · min-1. The LV detection wavelength was 282 nm. Six male SD rats were given a single dose of 50 mg · kg-1 imatinib by garage. Blood samples were collected from tail vein at different time points. The concentrations of imatinib in plasma were determined by the established HPLC method. The pharmacokinetic parameters were analyzed by DAS program. RESULTS: Excellent liner relationship was obtained in the range of 0.10-20.00 mg · L-1 (r=0.9998), and the lower limit of qauntification was 0.05 mg ·L-1. The recoveries were (98.86±2.77)%, (100.35±2.31)% and (100.14±1.66)% respectively at three concentrations (0.25, 5.00, 15.00 mg · L-1), the intra-day RSDs were 2.64%, 2.50% and 1.62% with REs of -1.60%, -1.00% and 0.10%, and the inter-day RSDs were 3.51%, 2.77% and 1.34% with REs of -0.80%, 0.72% and 0.21%, respectively. After oral administration in rats, the pharmacokinetic profile of imatinib was fitted with a two-compartment model, the half-lives of α phase and β phase were (2.40±0.84) h and (7.82±0.87) h, respectively. CONCLUSION: The method is accurate, simple, rapid and can be used to determine imatinib concentration in rat plasma and study its pharmacokinetics.

Objective To investigate the relationship between choroidal telangiectasia and subfoveal choroidal thickness in the patients with central serous chorioretinopathy.Methods Eighty-four patients (84 eyes) with central serous chorioretinopathy in our hospital from February 2015 to April 2016 were selected,based on the fluorescein angiography the patients were divided into 3 groups:Mild group (leakage at 9-10 minutes after injection,26 cases),moderate group (leakage at 5-8 minutes after injection,37 cases),severe group (leakage at 5 minutes after injection,21 cases).The choroidal capillary dilatation was assessed by OCTA,and subfoveal choroidal thickness in three groups and patients with different foveal choroidal expansion degree were measured by EDI-OCT,and correlation analysis was performed.Results The proportion of severe dilation of choriocapillae in mild group,moderate group,severe group were 7.69％,13.52％ and 23.81％,respectively.The subfoveal choroidal thickness was sequentially increased in mild group,moderate group,severe group (P ＜ 0.05).The subfoveal choroidal thickness in patients with mild,moderate and severe dilatation of choroidal capillaries were (306.59 ± 74.18) μm,(367.21 ± 85.04) μm and (416.27 ± 104.56) μm,respectively (P ＜ 0.05).The degree of choroidal telangiectasia in patients with central serous chorioretinopathy had a positive correlation with the thickness of subfoveal choroid (r =0.812,P =0.037).Conclusion The degree of choroidal telangiectasia and subchondral choroidal thickness in patients with central serous chorioretinopathy are significantly increased with the severity of the disease,and there is a positive correlation between them.