Objective To explore the efficacy of lamivudine and interferon in sequential therapy for chronic hepatitis B.Methods 92 cases of chronic hepatitis B patients were randomly divided into treatment group(n=46) and control group(n=46).In the treatment group,the patients were treated with lamivudine 100mg/d for 5 months,then combined with recombinant ?-2b interferon(IFN) 5Mu/dose,muscle injection,once per two days.Then the lamivudine was discontinued at sixth month and the patients were continuously treated with only interferon for 5 months.In the control group,the patients were treated with only lamivudine 100mg/d for 18 months.Results At the end of treatment,the normalization rate of ALT,HBV-DNA negative conversion rate and HBeAg/anti-HBe seroconversion rate were 93.5%,89.1% and 54.3% in the treatment group,then they were 82.6%,71.7% and 19.6% in the control group.Six months later,the sustained response rate of HBV-DNA conversion between treatment group and control group were 84.8% and 32.6%.Conclusion Lamivudine and interferon has better short-term efficacy in sequential therapy for chronic hepatitis B and it is worth further studying.