Objective To investigate the expression of MMP-9, p53 protein in primary hepatocarcinoma (PHC) and the relationship between the clinicpathological in PHC. Methods The expression of MMP-9 and p53 in 74 eases with PHC and microvessel density(MVD) were detected by immunohistochemistry,the clinical pathological parameters were also analyzed. Results In hepato-cellular carcinoma, the expression of MMP-9 and p53 were closely related to MVD, tumor size, aggressive behavior(vascular metastasis and tumor envelope) ( P < 0.05 ). The positive correlation was found between the expression of two factors in PHC. Conclusion MMP-9 and p53 might be impor-tant markers in the carcinogenesis,progression and prognosis of PHC.

Because of the exists of minimal residual disease(MRD), children with acute lymphoblastic leukemia (ALL) often suffer from recurrence. Dendritic cells (DC) play a pivotal role in the body's anti-tumor immune response through taking up tumor antigens to stimulate the body's immune system.Therefore it has become a hotspot due to DC's immunotherapy of clearing MRD in the body of children with ALL. This review discusses the DC characteristics, the immune escape mechanism associated with DC and the use of DC's immunotherapy in children with ALL.

Objective To explore the effect of process quality monitoring for hospital infection control of ICU. Methods A total of 265 critically ill patients admitted to ICU were chosen as the control group from April 1, 2014 to March 31, 2015, who were strictly enforced three cluster strategies to prevent catheter related blood stream infection (CRBSI), catheter-associated urinary tract infection (CAUTI) and ventilator–associated pneumonia (VAP) with periodical data monitoring in ICU. Another 292 critically ill patients admitted to ICU were chosen as the observation group from April 1, 2015 to March 31,2016, who were added 3 measures of process quality monitoring on the basis of implementation method of the control group. The incidence of CRBSI, CAUTI and VAP time were compared between two groups. Results There was statistically significant difference on the incidence rate of the CRBSI between the two groups (χ2=4.504, P=0.034), which was respectively 1.79%(5/280) and 5.10%(13/255). There was statistically significant difference in the incidence rate of CAUTI between the two groups (χ2=5.708, P=0.017), which was respectively 3.48% (10/287) and 8.27% (21/254). There was statistically significant difference on the incidence rate of VAP between the two groups (χ2=8.291, P=0.004), which was respectively 3.48% (10/287) and 8.27% (21/254). Mean time of mechanical ventilation in the observation group and the control group was respectively (2.14±1.40) days and (3.37±2.01) days, there was no significant difference (P=0.073). Mean time of ICU stay in the observation group and the control group was respectively (6.04±1.04) days and (11.92±1.90) days, there was statistical significance (t=-1.840, P=0.024). Number of qualified central venous catheter maintenance, perineum clean and oral care had negative relevance with number of patients with CRBSI, CAUTI and VAP, correlation coefficient in the observation group was respectively-0.701,-0.618,-0.677, there was statistical significance (P<0.05). Conclusions The implementation of quality monitoring can reduce the number of patients with CRBSI, CAUTI and VAP, which suggests wide application in ICU.

Objective To evaluate the therapeutic effects of dialysis regimen for multiple organ dysfunction syndrome(MODS) due to fish gall bladder poisoning.Methods 16 patients with acute renal failure,liver failure and heart failure caused by fish gall bladder poisoning were investigated.Results They were given synthetic treatments,especially dialysis.14 patients of them recovered and were discharged from the hospital without any complication;while 2 cases died.Conclusion Ingestion of fish gall bladder leads to kidney damage,as well as liver,heart and gastrointestinal tract injury.Immediate dialysis is the most effective treatment.

Glioma is the most common malignant brain tumor with high malignancy and lethality.The specific potential radiolabeled nanoparticles have been applied in the glioma research for non-invasive,dynamic,real-time and quantitative evaluation.Furthermore,radiolabeled nanoparticles have shown great potential in targeted therapy of glioma.The up-to-date application of radiolabeled nanoparticles in SPECT imaging,PET imaging,multimodality imaging and theranostics in glioma are reviewed in this article.

Objective:To investigate the efficacy and safety of peginterferon alpha-2a plus ribavirin in the treatment of chronic hepatitis C. Methods:Totally 85 patients with chronic hepatitis C were randomly divided into the observation group and the control group. Each patient in the observation group was injected subcutaneously peginterferon alpha-2a with the dose of 180μg once a week plus ribavirin 900-1 200 mg/d, po, and each patient in the control was treated subcutaneously with 5 MU standard interferon alpha-2b three times a week plus the same used ribavirin. After the 48-week treatment, 24-week fellow-up was carried out. HCV RNA and ALT were detected at the baseline, 4th week, 12th week and 48th week during the treatment, and 24th week after the treatment, respective-ly. The rate of rapid virological response ( RVR) , early virological response ( EVR) , end of treatment virological response ( ETVR) , sustained virological response (SVR), ALT normalization and adverse reactions were respectively assessed. Results: The rates of EVR,ETVR and SVR in the observation group were 76. 7%(33/43), 86. 0%(37/43) and 79. 1%(34/43), respectively, which were significantly higher than those in the control group [54. 8%(23/43), 66. 7%(28/43) and 57. 1%(24/43),respectively] (P<0. 05). The ALT normalization rate at 12th and 48th week after the treatment in the observation group was 81. 4% and 90. 7%, re-spectively, which were higher than those in the control group [64. 3% and 71. 4%, respectively](P<0. 05). The rate of white cell counts and platelet underlying (58. 1% and 39. 5%) in the observation group were also higher than those in the control group (35. 7%and 19.0%)(P<0.05). The decreased rate of neutrophil (58.1%) in the observation group was higher than that in the control group(19. 0%)(P<0. 01). The other adverse reactions in the two groups were similar, and there was no new or unique adverse event related to peginterferon. Conclusion:Peginterferon alpha-2a has better effect than the standard interferon alpha-2b in the treatment of patients with chronic hepatitis C with promising tolerance.

Objective To discusse the expression and clinic opathologic significance of vascular endothelial growth factor(VEGF) and microvessel density(MVD) in primary liver cancer,an immunohistochemical staining.Methods The expressions of VEGF and Ⅷ factor were determined in 60 selected operation specimen from patients with primary liver carcinoma.Results It showed that the positive expression rate of VEGF in primary liver cancer was 63.3%,the MVD was (30.76?11.56).Among them,the MVD in positive VEGF expression was (46.37?15.85),and the MVD in negative expression was (18.97?8.68),there were great statistical difference between them(P0.05).Conclusion This study indicates that VEGF can facilitate the formation of microvessel of the tumor and has correlation with the growth,infiltration and metastasis of primary liver cancer.

To clarify the clinical significance of alterations of Ha-ras, p53 and RB gene as well as infection with HPV16,18 in human bladder cancer, we examined the state of Ha-ras, p53 and RB genes, sequences of HPV16/18, and their association with clinicopathological parameters in 39 cases of bladder cancer and 7 cases of normal tissue, using nonisotopic PCR-SSCP and dot blot. The overall incidences of Ha-ras, p53 and RB gene mutation and HPV infection in tumor were 61.5% ,36% ,30.8% and 15.4% , respectively. The HPV positive rate was negatively correlated with clinical stage and pathological classification. Rather, the mutations of Ha-ras and p53 gene were positively correlated with the above clinical parameters. The incidence of Ha-ras gene mutation in recurring tumors was significantly higher than that in primary ones. A negative correlation between HPV infection and p53 mutation was also found. The results suggest that the above molecular events and their interaction play important roles in the development of bladder cancer, and that they wonld be of practical assistance in the prognosis and monitoring of bladder cancer.

Objective:To research the TLC identification method for Longdan Xiegan pills( honey pills) . Methods:TLC was used in the identification. The samples were extracted by 70% methanol with a heating reflux method, and then extracted by the agents with dif-ferent polarity, including petroleum ether, ethyl acetate and butanol. The petroleum ether part was detected by fluorescence at 365nm for Angelica sinensis, and 1% vanillin-sulfuric acid color reaction was used to detect Alisma orientale. The ethyl acetate part was determined by fluorescence at 365nm for Scutellaria baicalensis, and the butanol part was detected with chloroform-methanol-water (30∶12∶3) as the developing solvent for Bupleurum and Glycyrrhiza, and with acetone-ethyl acetate-water (6∶6∶1) as the developing solvent for gentiopi-croside, geniposide and liquiritin. Results:The developed TLC spots were clear with good separation, high specificity and promising re-producibility. Conclusion:The method can be exactly used in the qualitative identification and quality control of Longdan Xiegan pills ( honey pills) .

Objective To study the Val247Leu and Trp316Ser polymorphisms of β2-glycoprotein Ⅰ(β2GPⅠ) in systemic lupus erythematosus (SLE) patients and their associations with antiphospholipid antibodies and thrombotic complications.Methods We used DNA sequencing to detect the polymorphisms of Val247Leu and Trp316Ser in 378 SLE patients and 240 normal controls.Anti-β2GP Ⅰ antibodies and anticardiolipin (ACA) were tested by enzyme linked immunosorbent assay (ELISA).Lupus-type anticoagulants(LAC) was performed by diluted Russell's Viper Venom Test.Then the patient group was further analyzed according to APLs (Anti-β2GP Ⅰ antibody,LAC and ACA),thrombosis and obstetrical complications using Logistic regression analysis to confirm whether there are associations between β2GPⅠpolymorphism and those factors.Results For Va1247Leu,the predominant genotype was LL in healthy controls which accounted for 57.08％,while it was VL in SLE patients which accounted for 59.5％ (x2=45.01,P=0.000).Frequency of VV genotype was significantly higher in patients with thrombosis,anti-β2GP Ⅰ,ACA and obstetrical complications (OR=6.79,3.75,2.12 and 3.85,respectively;P=0.000,0.001,0.044 and 0.017,respectively).Those patients with VL genotype tended to have positive anti-β2GPI,LAC,ACA,thrombosis and also obstetrical complications (OR=2.95,1.88,2.47,2.97 and 2.74,respectively;P=0.000,0.007,0.000,0.001 and 0.016,respectively) than those negative ones.The predominant genotype of Trp316Ser was TT,then TS.No correlations could be found between Trp316Ser polymorphism and APLs,neither relation to thrombosis complications.Conclusion The polymorphism of Val247Leu is significantly associated with the presence of APLs,thrombosis and obstetrical complications.Both VV and VL genotype are risk factors for the generation of APLs,occurrence of thrombosis and obstetrical complications.The VV genotype is a high risk factor for thrombosis.Trp316Ser polymorphism does not contribute to the APLs production and also have no correlations with thrombotic complication.