Objective To understand the prevalence and awareness of Toxoplasma gondii of the pregnant women in Bazhou City,Hebei Province,so as to provide the evidence for formulating effective prevention and control interventions. Methods The pregnant women with prenatal screening in the Fourth People’s Hospital in Langfang City were selected and investigated with questionnaires. A part of the pregnant women were randomly sampled and received the ELISA test of the antibodies against T. gondii. Results Totally 672 pregnant women accepted the questionnaire survey,with an awareness rate of 19.49%(131/672). Totally 526 pregnant women of them were randomly selected to take the ELISA test,with a positive rate of 13.88%(73/526). The awareness rate and infection rate of T. gondii were related to the education level,occupation and place of residence (c2=5.89-17.62,all P<0.05),and the infection rate was negatively related to the awareness rate(c2=5.37,P<0.05). Con?clusion Increasing the awareness rate of toxoplasmosis knowledge is one of the effective methods to control the disease. There?fore,the health education about T. gondii should be carried out actively.

This study aimed at investigating the effects and mechanisms of 7-O-succinyl macrolactin A in inhibi-ting human lung cancer. After treatment of human lung cancer cell lines H460 with 7-O-succinyl macrolactin A, MTS assay was employed to determine cell proliferation;crystal violet staining was used to detect cell adhesion of H460;transwell chamber assay and wound healing assay were performed to evaluate cell invasion and migration;and flow cytometry assay was adopted to evaluate cell cycle. Western blotting and real-time PCR were also employed to determine the expression of β-catenin, c-Myc, Cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2 , Survivin and MMP-2/9. The phosphorylation of AKT and mTOR was determined as well. In vitro prolifera-tion of H460 was inhibited significantly by 7-O-succinyl macrolactin A. Cell adhesion, invasion and migration abilities were also attenuated. Western blot and real-time PCR showed that the expressions of β-catenin, c-Myc, cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9 were down-regulated by 7-O-succinyl macrolactin A. It was also found that phosphorylation of AKT and mTOR was inhibited by 7-O-succinyl macrolactin A. 7-O-succinyl macrolactin A can inhibit the in vitro growth and invasion of human lung cancer cell lines H460.

Objective To investigate the expressions and clinical significance of PPAR? and MMP-9 in the genesis and development of hypertensive disorder-complicating pregnancy(HDCP).Methods The expressions of PPAR? and MMP-9 in placenta of 31 normal pregnant women(control group) and 36 pregnant women with hypertensive disorder-complicating pregnancy(experimental group) were detected by using SP method of immunohistochemistry,and the relationship between PPAR? and MMP-9 expressions and hypertensive disorder-complicating pregnancy was explored.Results ① PPAR? expression in placental tissues was significantly higher in the experimental group than in the control group(P

BACKGROUND:Finite element contact analysis was performed in premolars of lower mandible with enamelo-dentinal junction defects, which provided biomechanical evidence for the prevention of wedge-shaped defect and occlusion treatment.
OBJECTIVE:To analyze the effects of undermining damage of the enamelo-dentinal junction on the stress distribution of sclerous tissues of dental cervix.
METHODS:Triangle defects were established and models of wedge-shaped defect were designed in mandibular premolar along the enamelo-dentinal junction. The stresses during tightly biting and masticatory movement were simulated. Nonlinear contact analysis was conducted in sclerous tissues of mandibular premolar in the buccal cervical region.
RESULTS AND CONCLUSION:By simulating the stresses during tightly biting and masticatory movement, the stresses were concentrated in the defected regions of mandibular premolar at the enamelo-dentinal junction. With the defect length increased in size, the stress value and the magnitude of the stress field both increased. These indicated that the destruction of enamelo-dentinal junction altered stress distribution in the buccal cervical region. In the clinic, wedge-shaped defect should be fil ed promptly.

Objective:To establish an HPLC method for the determination of isomeric impurity in fasudil hydrochloride. Meth-ods:The chromatographic method was carried out on a Kromasil 100-5 Phenyl C18 column with phenyl bonded silica as the filler (250 mm × 4. 6 mm, 5 μm), and phosphate buffer (10 mmol· L-1 ammonium dihydrogen phosphate, adjusting pH to 4. 0 with 1% phos-phoric acid) -acetonitrile (80∶ 20) was used as the mobile phase. The detection wavelength was 275nm and the column temperature was 40℃. The flow rate was 1. 0 ml· min-1 , and the injection volume was 10 μl. Results:Fasudil hydrochloride and its derivative was linear within the range of 0. 148-2. 960 μg·ml-1(r=1. 0000) and 0. 101-2. 014μg·ml-1(r=0. 999 9), respectively. The av-erage recovery of isomer impurity in fasudil hydrochloride was 101. 9% with RSD of 0. 98%(n=9). Conclusion:The method is sim-ple,accurate and reproducible,which can be used for the quality control of fasudil hydrochloride and its isomer.

BACKGROUND: Olfactory ensheathing cell (OEC) transplantation can promote the recovery of neurological function in rats with cerebral infarction, while the migratory pattern of transplanted OECs and the relationship between OECs migrated to various encephalic regions and plasticity upregulation and recovery of neurological function of the encephalic region are still unknown.OBJECTIVE: To observe the migratory pattern and therapeutic value of OEC transplantation in rats with cortical cerebral infarction.DESIGN, TIME AND SETTING: Randomized, controlled, cell transplantation observation experiment was conducted between June 2002 and January 2004 at the Laboratory of Department of Neurology, the First Affiliated Hospital of Sun Yet-San University and Animal Experimental Center of SUN YET-SEN University, Guangzhou, Guangdong Province, China.MATERIALS: Sprague-Dowley rats, 2.5-month-old, were used for olfactory ensheathing cell culture. 150 Sprague-Dowley rats,60-90 days, were used to replicate stroke-prone reuovascular hypertensive rat model using two-kidney two-clip method.METHODS: We purified OECs with differential time adherent method and labeled OECs with Hoechst 33342 before transplantation. Seventy stroke-prone renovascular hypertensive rats were used to prepare middle cerebral artery occlusion model.model rats were randomly allocated to 3 groups to receive OEC transplantation: peri-mfarct cortex transplantation group,contralateral cortex transplantation group and bilateral transplantation group.MAIN OUTCOME MEASURES: The recovery of motor and sensory function was observed at 2 weeks and 6 weeks after transplantation with behavior and sensory function examination; the survival and distribution conditions of transplanted ceils were observed under the fluorescence microscope.RESULTS: The recovery condition of motor and sensory function of rats in bilateral transplantation group was obviously better than that of rats in peri-infarct cortex transplantation group and contralateral cortex transplantation group (P ＜ 0.01), nerve fiber number and positive signal value of growth associated protein-43 in the marginal zone of cerebral infarction were also more than that in peri-infarct cortex transplantation group and contralateral cortex transplantation group. Transplanted cells in peri-infarct cortex transplantation group migrated to infarct and contralateral cortex along corpus callosum, transplanted cells in contralateral cortex transplantation group migrated to midline and infarct cortex along corpus callosum, transplanted ceils in bilateral transplantation group migrated along corpus callosum and could be seen in bilateral cortices while more in infarct cortex.CONCLUSION: Transplanted OECs can survive for a long time period, and these cells not only confine to injection point but also can migrate to infarct and contralateral cortex along corpus caliosum to promote the recovery of neurological function of rats with cerebral infarction, the effect is more significant in bilateral transplantation.

Objective To observe the time-intensity curve characteristics of contrast agents in intraocular tumor.Methods A total of 236 patients (238 eyes) with intraocular tumor were enrolled in this study.All the patients received regular ophthalmologic examination,two dimensional ultrasound,color doppler ultrasonography and contrast-enhanced ultrasonography.There were 166 patients (166 eyes) with choroidal melanoma,16 patients (18 eyes) with choroidal metastatic carcinoma,52 patients (52 eyes) with choroidal hemangioma,two patients (two eyes) with retinal hemangioma.The whole process of contrast-enhanced ultrasound were recorded, and exported as t images of Dicom format.These images were processed by Sonoliver software (Tomteck Company, Germany) to drawn the time-intensity curve of contrast agents in the intraocular tumors.Results All intraocular lesions were completely filled with contrast agent,concentric filling from the periphery to the center can be documented in some cases.The time-intensity curve of choroidal hemangioma and retinal hemangioma were basically the same.The time-intensity curve of choroidal melanoma and choroidal metastatic carcinoma were also basically the same.In the filling phase,all tumors were rapid filling type.In the regression phase,contrast agent subsided earlier than in control tissue within the melanoma or metastatic carcinoma lesions,but subsided synchronous or slightly faster than in control tissue within the choroidal hemangioma and retinal hemangioma lesions.Among 166 eyes with choroidal melanoma,138 eyes (83.1％) were in full compliance with the above changes,28 eyes (16.9％) were largely in line with these changes.All the eyes (100.0％) with choroidal metastatic carcinoma,choroidal hemangioma and retinal hemangioma were in full compliance with the above changes.Conclusion Time-intensity curve is quickly filling and fast regression for malignant intraocular tumors,but is quickly filling and slow regression for benign intraocular tumors.

Objective:To study the stress distribution of the mandibular premolar cervix during clenching and mastication. Methods:Models of anisotropic enamel of the mandibular first premolar and the opposite maxillary first premolar crown were establishied. The stress distributions in the cervical region of the mandibular premolar were investigated in different loading conditions: simulating working, nonworking and vertical micromotions. In each case, the stroke was applied to the maxillary first mandibular premolar in a stepping procedure using nonlinear contact analysis. Results:The stress in the cervical region under loading condition simulating vertical micromotion was lower than that under loading conditions simulating working and nonworking micromotions. The stress was mainly concentrated near the buccal or the lingual cervical enamelo-dentinal junction under loading conditions simulating lateral excursion. Conclusion:The stress distribution in the cervical region is closely related to the pattern of occlusal force including location and direction.

Objective To discuss the diagnosis characteristics and clinical value of posterior vitreous detachment (PVD) by color Doppler flow imaging(CDFI). Methods One thousand and two cases were examed by CDFI for the diagnosis standard and property of PVD. Results Typical acoustic properties of PVD: the B scan may show a very fluid thick line. There were two types complete PVD and part PVD based on the vitreous separated from the optic nerve. Kinetic echography typically showed a very fluid, undulating after movement of the completely PVD. Part PVD had more than one dot with attachments to the retina and less mobile than complete PVD. There was not blood flow signal on the PVD by CDFI. Conclusions Ultrasonography for PVD not only exactitudes,but also provides foundation for clinic.

Objective To study the relationship between fetal growth retardation(FGR) and expression of insulin-like growth factor-2 and H19 imprinted genes.Methods Immunoradioassay was used to detect the insulin-like growth factor-2(IGF2)level in cord blood from 42 pregnant women with FGR(FGR group) and 30 normal pregnant women(normal group).Reverse transcription-polymerase chain reaction method(RT-PCR) was used to detect H19 imprinted genes allele-specific expression level in placenta.Results The level of IGF2 in cord blood from FGR group was(1.52?0.20)?g/L.It was significantly decreased as compared with that from the normal group(1.97?0.21)?g/L(P