Objective To investigate the expressions and clinical significance of PPAR? and MMP-9 in the genesis and development of hypertensive disorder-complicating pregnancy(HDCP).Methods The expressions of PPAR? and MMP-9 in placenta of 31 normal pregnant women(control group) and 36 pregnant women with hypertensive disorder-complicating pregnancy(experimental group) were detected by using SP method of immunohistochemistry,and the relationship between PPAR? and MMP-9 expressions and hypertensive disorder-complicating pregnancy was explored.Results ① PPAR? expression in placental tissues was significantly higher in the experimental group than in the control group(P

This study aimed at investigating the effects and mechanisms of 7-O-succinyl macrolactin A in inhibi-ting human lung cancer. After treatment of human lung cancer cell lines H460 with 7-O-succinyl macrolactin A, MTS assay was employed to determine cell proliferation;crystal violet staining was used to detect cell adhesion of H460;transwell chamber assay and wound healing assay were performed to evaluate cell invasion and migration;and flow cytometry assay was adopted to evaluate cell cycle. Western blotting and real-time PCR were also employed to determine the expression of β-catenin, c-Myc, Cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2 , Survivin and MMP-2/9. The phosphorylation of AKT and mTOR was determined as well. In vitro prolifera-tion of H460 was inhibited significantly by 7-O-succinyl macrolactin A. Cell adhesion, invasion and migration abilities were also attenuated. Western blot and real-time PCR showed that the expressions of β-catenin, c-Myc, cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9 were down-regulated by 7-O-succinyl macrolactin A. It was also found that phosphorylation of AKT and mTOR was inhibited by 7-O-succinyl macrolactin A. 7-O-succinyl macrolactin A can inhibit the in vitro growth and invasion of human lung cancer cell lines H460.

Objective To understand the prevalence and awareness of Toxoplasma gondii of the pregnant women in Bazhou City,Hebei Province,so as to provide the evidence for formulating effective prevention and control interventions. Methods The pregnant women with prenatal screening in the Fourth People’s Hospital in Langfang City were selected and investigated with questionnaires. A part of the pregnant women were randomly sampled and received the ELISA test of the antibodies against T. gondii. Results Totally 672 pregnant women accepted the questionnaire survey,with an awareness rate of 19.49%(131/672). Totally 526 pregnant women of them were randomly selected to take the ELISA test,with a positive rate of 13.88%(73/526). The awareness rate and infection rate of T. gondii were related to the education level,occupation and place of residence (c2=5.89-17.62,all P<0.05),and the infection rate was negatively related to the awareness rate(c2=5.37,P<0.05). Con?clusion Increasing the awareness rate of toxoplasmosis knowledge is one of the effective methods to control the disease. There?fore,the health education about T. gondii should be carried out actively.

BACKGROUND:Finite element contact analysis was performed in premolars of lower mandible with enamelo-dentinal junction defects, which provided biomechanical evidence for the prevention of wedge-shaped defect and occlusion treatment.
OBJECTIVE:To analyze the effects of undermining damage of the enamelo-dentinal junction on the stress distribution of sclerous tissues of dental cervix.
METHODS:Triangle defects were established and models of wedge-shaped defect were designed in mandibular premolar along the enamelo-dentinal junction. The stresses during tightly biting and masticatory movement were simulated. Nonlinear contact analysis was conducted in sclerous tissues of mandibular premolar in the buccal cervical region.
RESULTS AND CONCLUSION:By simulating the stresses during tightly biting and masticatory movement, the stresses were concentrated in the defected regions of mandibular premolar at the enamelo-dentinal junction. With the defect length increased in size, the stress value and the magnitude of the stress field both increased. These indicated that the destruction of enamelo-dentinal junction altered stress distribution in the buccal cervical region. In the clinic, wedge-shaped defect should be fil ed promptly.

Objective To discusse the expression and clinic opathologic significance of vascular endothelial growth factor(VEGF) and microvessel density(MVD) in primary liver cancer,an immunohistochemical staining.Methods The expressions of VEGF and Ⅷ factor were determined in 60 selected operation specimen from patients with primary liver carcinoma.Results It showed that the positive expression rate of VEGF in primary liver cancer was 63.3%,the MVD was (30.76?11.56).Among them,the MVD in positive VEGF expression was (46.37?15.85),and the MVD in negative expression was (18.97?8.68),there were great statistical difference between them(P0.05).Conclusion This study indicates that VEGF can facilitate the formation of microvessel of the tumor and has correlation with the growth,infiltration and metastasis of primary liver cancer.

Objective To compare the accuracy of intraocular lens(IOL)power calculations by using 6 formulas(SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ)in different axial lengths of the eye,in order to improve the accuracy of predicating IOL powers for patients after cataract surgery.Design Retrospective comparative clinical analysis.Participants 169 patients(169 eyes)with age-related cataract underwent regular phacoemulsification and posterior chamber IOL implantation.Methods 169 eyes with cataract were divided into five groups according to the axial lengths:AXL≤22mm,22mm28.4mm.The axial lengths and keratometry of the 169 eyes were measured with A-mode ultrasound before operation,and the actual postoperative refraction was measured with the methods of computer and retinoscopy optometry when patients could gain best corrected visual acuity one month after surgery or later.Inputting the axial lengths,keratometry and IOL powers into A-mode ultrasound,the predicted postoperative refraction was calculated with SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ formulas,and the differences of predicted and actual postoperative refractions in different axial lengths were compared.Main Outcome Measures The absolute value with the differences of the predicted and actual postoperative refractions,i.e.absolute predicted errors.Results(1)Comparing the difference of predicted and actual postoperative refraction,there was no statistical significance between the five formulas in the groups of axial length(AXL)≤22mm,22mm28.4mm,but the average absolute predicted error of Haigis and SRK-T formulas is significantly smaller than SRK-Ⅱand Binkhorst-Ⅱ formulas.Conclusions In existing sample size,there was no obvious influence on postoperative refraction when using other five formulas except Binkhorst-Ⅱ.But in the extremely long AXL group,Haigis and SRK-T formulas have some ascendancy over the other formulas.

Objective: To evaluate the initial pain and influential factors in orthodontic treatment by VAS(visual analogue scales),and explore the way to alleviate the pain. Methods:107 subjects(47 male and 60 female) were selected. Following insertion of the first archwire (0.36 mm Chinese NiTi wire), visual analogue scale(VAS) was used to record the severity of pain at 4,12,24 h and on day 3,5,7. All the subjects were randomly divided into two groups by ligature with elastomeric rings (in 51 patients) or stainless steel wires (in 56 patients).In all the 107 patients, 30 people need to extract their tooth for relieving crowding while the other 77 were non-extraction cases. Results:No significant differences of pain were found between elastomeric rings ligature cases and stainless steel wires ligature cases at each stage; as well as between extraction and non-extraction cases. But the adults perceived more pain than the adolescents. Patients who were asked to chew gum or to do something (watching TV or listening to music) for distraction perceived less pain.The differences were statistically significant between different groups. Conclusion:The orthodontist should make a good communication with patient about initial pain before the orthodontic treatment, especially for adults.

BACKGROUND: Olfactory ensheathing cell (OEC) transplantation can promote the recovery of neurological function in rats with cerebral infarction, while the migratory pattern of transplanted OECs and the relationship between OECs migrated to various encephalic regions and plasticity upregulation and recovery of neurological function of the encephalic region are still unknown.OBJECTIVE: To observe the migratory pattern and therapeutic value of OEC transplantation in rats with cortical cerebral infarction.DESIGN, TIME AND SETTING: Randomized, controlled, cell transplantation observation experiment was conducted between June 2002 and January 2004 at the Laboratory of Department of Neurology, the First Affiliated Hospital of Sun Yet-San University and Animal Experimental Center of SUN YET-SEN University, Guangzhou, Guangdong Province, China.MATERIALS: Sprague-Dowley rats, 2.5-month-old, were used for olfactory ensheathing cell culture. 150 Sprague-Dowley rats,60-90 days, were used to replicate stroke-prone reuovascular hypertensive rat model using two-kidney two-clip method.METHODS: We purified OECs with differential time adherent method and labeled OECs with Hoechst 33342 before transplantation. Seventy stroke-prone renovascular hypertensive rats were used to prepare middle cerebral artery occlusion model.model rats were randomly allocated to 3 groups to receive OEC transplantation: peri-mfarct cortex transplantation group,contralateral cortex transplantation group and bilateral transplantation group.MAIN OUTCOME MEASURES: The recovery of motor and sensory function was observed at 2 weeks and 6 weeks after transplantation with behavior and sensory function examination; the survival and distribution conditions of transplanted ceils were observed under the fluorescence microscope.RESULTS: The recovery condition of motor and sensory function of rats in bilateral transplantation group was obviously better than that of rats in peri-infarct cortex transplantation group and contralateral cortex transplantation group (P ＜ 0.01), nerve fiber number and positive signal value of growth associated protein-43 in the marginal zone of cerebral infarction were also more than that in peri-infarct cortex transplantation group and contralateral cortex transplantation group. Transplanted cells in peri-infarct cortex transplantation group migrated to infarct and contralateral cortex along corpus callosum, transplanted cells in contralateral cortex transplantation group migrated to midline and infarct cortex along corpus callosum, transplanted ceils in bilateral transplantation group migrated along corpus callosum and could be seen in bilateral cortices while more in infarct cortex.CONCLUSION: Transplanted OECs can survive for a long time period, and these cells not only confine to injection point but also can migrate to infarct and contralateral cortex along corpus caliosum to promote the recovery of neurological function of rats with cerebral infarction, the effect is more significant in bilateral transplantation.

Objective To discuss the diagnosis characteristics and clinical value of posterior vitreous detachment (PVD) by color Doppler flow imaging(CDFI). Methods One thousand and two cases were examed by CDFI for the diagnosis standard and property of PVD. Results Typical acoustic properties of PVD: the B scan may show a very fluid thick line. There were two types complete PVD and part PVD based on the vitreous separated from the optic nerve. Kinetic echography typically showed a very fluid, undulating after movement of the completely PVD. Part PVD had more than one dot with attachments to the retina and less mobile than complete PVD. There was not blood flow signal on the PVD by CDFI. Conclusions Ultrasonography for PVD not only exactitudes,but also provides foundation for clinic.

Objective To study the relationship between fetal growth retardation(FGR) and expression of insulin-like growth factor-2 and H19 imprinted genes.Methods Immunoradioassay was used to detect the insulin-like growth factor-2(IGF2)level in cord blood from 42 pregnant women with FGR(FGR group) and 30 normal pregnant women(normal group).Reverse transcription-polymerase chain reaction method(RT-PCR) was used to detect H19 imprinted genes allele-specific expression level in placenta.Results The level of IGF2 in cord blood from FGR group was(1.52?0.20)?g/L.It was significantly decreased as compared with that from the normal group(1.97?0.21)?g/L(P