1.Mobile Health: IEEE Standard for Wearable Cuffless Blood Pressure Measuring Devices.
Xia ZHOU ; Wenli WU ; Shudi BAO
Chinese Journal of Medical Instrumentation 2015;39(4):285-287
IEEE Std 1708-2014 breaks through the traditional standards of cuff based blood pressure measuring devices and establishes a normative definition of wearable cuffless blood pressure measuring devices and the objective performance evaluation of this kind of devices. This study firstly introduces the background of the new standard. Then, the standard details will be described, and the impact of cuffless blood pressure measuring devices with the new standard on manufacturers and end users will be addressed.
Blood Pressure
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Blood Pressure Monitors
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standards
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Humans
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Reference Standards
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Telemedicine
2.Preparation of long oligonucleotide microarray for detection and sub-typing of human papillomavirus
Min WEI ; Wenli MA ; Bao ZHANG
Medical Journal of Chinese People's Liberation Army 1983;0(02):-
Objective To design a microarray of ~60mer oligonucleotide for detection and sub-typing of human papillomavirus (HPV). Methods The type-specific oligonucleotide probes of 4 different types of HPV (6, 11, 16, 18) were designed by using biological software Arraydesigner 2.0, which analyzed the whole genome sequences of HPV and selected optimal probes with high specificity, identical length and similar melting temperature (Tm). These probes were synthesized and printed onto the surface of glass slides in order to prepare a low-density microarray. HPV samples were labeled with fluorescence dyes Cy3 using a method of restriction display PCR (RD-PCR). HPV plasmid DNA was restricted with Sau3AⅠ to produce multiple fragments which were ligated to adaptors subsequently and used as PCR template. PCR labeling was performed with the fluorescently labeled universal primer (Cy3-UP) whose sequence is designed according to the adaptor of the RD-PCR approaches. The labeled samples were then hybridized with the oligonucleotide microarray. Results Both single and multiple HPV DNA samples could be detected with oligonucleotides microarray, and the corresponding HPV subtypes were recognized as well. And no signals were detected in all the negative and blank control spots. Conclusion 60mer oligonucleotide microarray designed by appropriate bioinformatics software can be applied to HPV detection and genotyping on gene level.
3.Preliminary study on value of lymphocyte function detection in diagnosis and therapy of Henoch-Schonlein purpura
Lijuan BAO ; Wenli LI ; Lei PI ; Baidu ZHANG
International Journal of Laboratory Medicine 2017;38(7):890-891,894
Objective To investigate the changes of peripheral blood lymphocyte function in the children patients with acute phase of Henoch-Schonlein purpura(HSP),and to explore its significance in the diagnosis and therapy of HSP.Methods The peripheral blood lymphocytes subsets CD3+,CD4+,CD8+,CD4+/CD8+,CD19+ and NK cells(CD16++ CD56+) were detected in 51 children patients with acute phase of HSP and 40 children undergoing physical examination by flow cytometry(FCM Results Compared with the control group,the counts of peripheral blood CD4+,CD4+/ CD8+and NK cells CD16++ CD56+) in the acute stage of the HSP group were significantly decreased(P<0.01),while the proportions of CD8+ and CD19+cells were significantly increased(P<0.05,P<0.01).Conclusion The immunity dysfunction exists in the children patients with acute phase of HSP.The peripheral blood lymphocyte function detection has an important significance for the early diagnosis and treatment of HSP.
4.Influence of Maca on Resist-fatigue and T-SOD, Liver Glycogen of the Mice After Exhaustive Sports
Liangwu QIU ; Lu WANG ; Li WANG ; Chunling BAI ; Wenli BAO
Journal of Kunming Medical University 2016;37(6):18-20
Objective To establish the resist-fatigue animal test through using Maca, in order to provide reference for the relevant departments to exploit new sports nutrition . Methods Thirty male KM mice were randomly divided into Quiet control group (A),Exhaustive group (B,high altitude),Maca exhaustive group (C), there were 10 mice in each group. Drinking water containing Maca (Maca =500 mg/L) was supplied in group C. Additional exhaustive group (D,low altitude) was set.Thirty days later, all mice were killed after one exhaustive test, then we tested the exhaustive time, the level of T-SOD and liver glycogen. Results The exhaustive time of group C was significantly longer than group B (P<0.05), the T-SOD level of group C was significantly higher than group A and group B (P<0.05), and the liver glycogen content of group C was significantly higher than group B (P<0.05) . Conclusion Maca can raise T-SOD and liver glycogen level of the mice after exhaustive swimming, and has function of resist-fatigue.
5.Expression of Rabies Virus Glycoprotein Gene in Saccharomyces cerevisiae
Hui ZHAO ; Wenling ZHENG ; Yang GAO ; Jinfang ZHANG ; Yifei PENG ; Bao ZHANG ; Wenli MA
Microbiology 2008;0(11):-
To obtain non-pathogenic rabies virus glycoprotein(RV-G),we expressed RV-G in Saccaromyces Cerevisiae(S.cerevisiae).In our study,tat-G fusion gene was cloned into the expression vector pYes2.0,which allows expression of a foreign gene in the yeast cells under the control of GAl1 promoter.Transforma-tion was performed by using lithium-treated yeast cells and several Ura+-tranformants were isolated.Ac-cording to the relative mobility in SDS-PAGE,we know probably two forms(designated as yGI and yGⅡ) of RV-G analogues produced in S.cerevisiae,their molecular weights were estimated as 66 kD and 56 kD,respectively.On the other hand,there was a specific band about 56 kD shown in western blot result.Com-bining precursors’ achievements,we will draw a conclusion that trans-membrane domain(TD) and cyto-plasmic domain have a negative regulation on RV-G antigen immunogenicity in S.cerevisiae.
6.Ethical issues in clinical laboratory medicine research
Xiaoqiong GU ; Baidu ZHANG ; Wenli LI ; Haifeng LONG ; Lijuan BAO ; Yuan ZHANG
Chinese Journal of Medical Science Research Management 2016;29(1):14-16
Biomedical research involving human body needs to be reviewed and oversight by the Institute Review Board (IRB) is the important international rule,aimed to protect human subjects.Ethics,however,may limited the freedom and innovation of medical research.It is an important task for the medical researchers on how to make the medical research to meet the demands of ethics,to avoid the risk,and to promote the innovation for the better development of the medical research.
7.Effect of propofol on JAK2/STAT3 signaling pathway in hippocampus of rats undergoing orthotopic liver transplantation
Lili JIA ; Yongjuan BAO ; Fei WANG ; Yiqi WENG ; Wenli YU ; Hongyin DU
Chinese Journal of Anesthesiology 2016;36(6):720-724
Objective To evaluate the effect of propofol on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in the hippocampus of rats undergoing orthotopic liver transplantation.Methods Twenty-four healthy male Sprague-Dawley rats,aged 8-10 weeks,weighing 220-250 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S);orthotopic liver transplantation group (group O);propofol group (group P).After the rats were anesthetized with chloral hydrate,the model of orthotopic liver transplantation was established according to the method described by Nozato et al.in O and P groups.In group P,30 min infusion of propofol was started at a rate of 20 mg · kg-1 · h-1 via the right femoral vein immediately after onset of reperfusion.At 6 h of reperfusion,blood samples were collected from the infrahepatic vena cava,and then the rats were sacrificed.The hippocampi were harvested for determination of malondialdehyde (MDA)and nitric oxide (NO) contents and superoxide dismutase (SOD) activity.The serum levels of S100β protein and neuron-specific enolase (NSE) were determined by enzyme-linked immunosorbent assay.The expression of JAK2,STAT3 and inducible nitric oxide synthase (iNOS) mRNA was detected by quantitative real-time polymerase chain reaction.The pathological changes of hippocampal tissues were examined under the light microscope,and the cell apoptosis was measured using TUNEL.The apoptotic index was calculated.Results Compared with group S,the serum levels of S100β protein and NSE were significantly increased,the contents of MDA and NO were significantly increased,the activity of SOD was significantly decreased,the expression of JAK2,STAT3 and iNOS mRNA was significantly up-regulated,the apoptotic index was significantly increased (P<0.05),and the pathological changes of hippocampal tissues were significantly aggravated in the other groups.Compared with group O,the serum levels of S100β protein and NSE were significantly decreased,the contents of MDA and NO were significantly decreased,the activity of SOD was significantly increased,the expression of JAK2,STAT3 and iNOS mRNA was significantly downregulated,the apoptotic index was significantly decreased (P<0.05),and the pathological changes of hippocampal tissues were significantly reduced in P group.Conclusion Propofol reduces injury to the hippocampus through blocking JAK2/STAT3 signaling pathway in the rats undergoing orthotopic liver transplantation.
8.Effects of four drying methods after mechanical cleaning of dental handpiece
Wenli BAO ; Yuqun WANG ; Ying YE
Chinese Journal of Nursing 2018;53(3):318-320
Objective To compare drying methods for dental handpiece,and to provide references and guidance for choosing appropriate handpiece drying methods.Methods Four groups were established according to different methods of drying:vacuum drying,compressed air dryer drying,entire automatic hot cleaner drying,and sterilizing machine drying.Four types (with 50 in each type)of handpieces with the same brand were selected:low-speed straight (EX-6C),low-speed bender (NAC-EC),high speed turbine fast handset,and high speed turbine four hole handset.After cleaning and disinfection,each handpiece was subjected to the aforementioned four drying methods,and the mass was recorded before and after drying.SPSS software was used to calculate and compare the drying rate among these drying methods by analysis of variance.Results There were significant differences in the drying rate among four drying methods(P<0.01).The drying efficiency with vacuum drying was higher than the other three methods.The mean drying efficiency for four types of dentistry handset with vacuum drying method were as follows:low-speed straight(EX-6C)69.93%,low-speed bender(NAC-EC)93.21%,high speed turbine fast handset 92.70%,and high speed turbine four hole handset 94.27%.Conclusion The drying efficiency of vacuum drying method for handset is superior to the other three drying methods.
9.Effect of poly adenosine diphosphate-ribose polymerase-1 inhibitor fluzoparib on the proliferation and migration of pancreatic cancer cell PANC1
Naiyu QIN ; Minxue XU ; Jie WU ; Wenjie ZHENG ; Wenli SAI ; Lingling PAN ; Feng JIANG ; Mingbing XIAO ; Baijun BAO
Chinese Journal of Pancreatology 2022;22(2):118-122
Objective:To investigate the effects of poly adenosine diphosphate ribose polymerase-1(PARP-1) inhibitor fluzoparib on proliferation, apoptosis and migration of pancreatic cancer PANC1 cells.Methods:PANC1 cells cultured in conventional culture medium were used as control group, and PANC1 cells cultured in the medium containing fluzoparib were used as fluzoparib group. The effects of fluzoparib with different concentrations on the proliferation of PANC1 cells were detected by CCK8 method, and the half inhibitory concentration (IC 50) of fluzoparib on PANC1 cells was calculated. The effect of fluzoparib on apoptosis and cell cycle of PANC1 cells was detected by flow cytometry, and the migration ability of PANC1 cells was detected by cell scratch test and Transwell chamber. Results:Compared with control group, with the increase of fluzoparib concentration and the prolongation of the action time, the cell proliferation activity of PANC1 in fluzoparib group was significantly decreased, and the differences were statistically significant (all P values <0.05). IC 50 of fluzoparib on PANC1 cells cultured for 24 h was 0.03 mmol/L. After 24 h culture, the IC 50 apoptosis rate of fluzoparib group was (32.19±2.48)%, and the apoptosis rate of control group was (21.99±6.30)%. The former was greatly higher than the latter, and the difference was statistically significant ( P<0.05). The proportion of cells in G 2/M phase was (16.28±0.62)% in the fluzoparib group and (11.64±0.88)% in the control group, and the difference between the two groups was statistically significant ( P<0.05). The migration rates of PANC1 cells in IC 50 fluzoparib group in 12 h and 24 h culture were (2.59±1.46)% and (19.76±7.84)%; and those in control group were (27.08±2.17)% and (45.92±3.61)%, respectively. The number of transmembrane cells was (348±19) cells/10 visual field in the fluzoparib group and (587±14) cells/10 visual field in the control group. The migration ability of PANC1 cells in fluzoparib group was significantly lower than that in control group ( P<0.05). Conclusions:Fluzoparib can inhibit the proliferation and migration of PANC1 cells and promote the apoptosis of PANC1 in vitro, which may be an effective drug for the treatment of pancreatic cancer.
10.Clinical characteristics of 272 437 patients with different histopathological subtypes of primary esophageal malignant tumors
Lidong WANG ; Liuyu LI ; Xin SONG ; Xueke ZHAO ; Fuyou ZHOU ; Ruihua XU ; Zhicai LIU ; Aili LI ; Jilin LI ; Xianzeng WANG ; Liguo ZHANG ; Fangheng ZHU ; Xuemin LI ; Weixing ZHAO ; Guizhou GUO ; Wenjun GAO ; Xiumin LI ; Lixin WAN ; Jianwei KU ; Quanxiao XU ; Fuguo ZHU ; Aifang JI ; Huixiang LI ; Jingli REN ; Shengli ZHOU ; Peinan CHEN ; Qide BAO ; Shegan GAO ; Haijun YANG ; Jinchang WEI ; Weimin MAO ; Zhanqiang HAN ; Zhiwei CHANG ; Yingfa ZHOU ; Xuena HAN ; Wenli HAN ; Lingling LEI ; Zongmin FAN ; Ran WANG ; Yuanze YANG ; Jiajia JI ; Yao CHEN ; Zhiqiang LI ; Jingfeng HU ; Lin SUN ; Yajie CHEN ; Helin BAI ; Duo YOU
Chinese Journal of Internal Medicine 2022;61(9):1023-1030
Objective:To characterize the histopathological subtypes and their clinicopathological parameters of gender and onset age by common, rare and sparse primary esophageal malignant tumors (PEMT).Methods:A total of 272 437 patients with PEMT were enrolled in this study, and all of the patients were received radical surgery. The clinicopathological information of the patients was obtained from the database established by the State Key Laboratory of Esophageal Cancer Prevention & Treatment from September 1973 to December 2020, which included the clinical treatment, pathological diagnosis and follow-up information of esophagus and gastric cardia cancers. All patients were diagnosed and classified by the criteria of esophageal tumor histopathological diagnosis and classification (2019) of the World Health Organization (WHO). The esophageal tumors, which were not included in the WHO classification, were analyzed separately according to the postoperative pathological diagnosis. The χ 2 test was performed by the SPSS 25.0 software on count data, and the test standard α=0.05. Results:A total of 32 histopathological types were identified in the enrolled PEMT patients, of which 10 subtypes were not included in the WHO classification. According to the frequency, PEMT were divided into common (esophageal squamous cell carcinoma, ESCC, accounting for 97.1%), rare (esophageal adenocarcinoma, EAC, accounting for 2.3%) and sparse (mainly esophageal small cell carcinoma, malignant melanoma, etc., accounting for 0.6%). All the common, rare, and sparse types occurred predominantly in male patients, and the gender difference of rare type was most significant (EAC, male∶ female, 2.67∶1), followed with common type (ESCC, male∶ female, 1.78∶1) and sparse type (male∶ female, 1.71∶1). The common type (ESCC) mainly occurred in the middle thoracic segment (65.2%), while the rare type (EAC) mainly occurred in the lower thoracic segment (56.8%). Among the sparse type, malignant melanoma and malignant fibrous histiocytoma were both predominantly located in the lower thoracic segment (51.7%, 66.7%), and the others were mainly in the middle thoracic segment.Conclusion:ESCC is the most common type among the 32 histopathological types of PEMT, followed by EAC as the rare type, and esophageal small cell carcinoma and malignant melanoma as the major sparse type, and all of which are mainly occur in male patients. The common type of ESCC mainly occur in the middle thoracic segment, while the rare type of EAC mainly in the lower thoracic segment. The mainly sparse type of malignant melanoma and malignant fibrous histiocytoma predominately occur in the lower thoracic segment, and the remaining sparse types mainly occur in the middle thoracic segment.