Objective To evaluate the saf ety and efficiency of phacoemulsificatio n combined with intraocular lens implant and trabeculectomy in patients with glau coma and cataract after filtrating surge ry failure. Methods Thirty-two cases （32 eyes） patients underwent phacoemulsific ation combined with intraocular lens imp lant and trabeculectomy after filtrating surgery failure. Outcome measures inclu ded intraocular pressure （IOP） and visua l acuity. The followed-up periods rang ed from 6 months to 2 years. Results In 21 of the 32 eyes（66％），postoperative vi sual acuity were above 0.5. In 6 of the 32 eyes（18％），postoperative visual acuity were between 0.1 to 0.4. In 27 of 32 e yes（84.3％）， postoperative IOP were between 12.23 to 20.55 mmHg. Conclusion phac oemulsification combined with intraocula r lens implant and trabeculectomy in patients with glaucoma and cataract after f iltrating surgery failure is a safe and effective treatment for these patients.

Objective:According to the theory of Traditional Chinese Medicine pulse diagnosis, we have used a PVDF pulse sensors and a combination of pre-amplifier unit to accurately detect the pulse information.Methods: Based on the principle of electronic technology design a multi-level amplification and filtering circuitry for detecting the pulse signal condition. Design the data acquisition circuit based on AT89C51 microcontroller and analog-digital conversion chip AD0809.Results: Using the signal acquisition and data amplifier circuit to effectively collect, process and amplify the pulse wave, will integrally combine the traditional Chinese medicine theory and modern scientific theories, greatly reduce the misdiagnosis rate, then effectively achieve the objective of pulse diagnosis.Conclusion: The data acquisition circuit which based on AT89C51 single-chip microcomputer and A/D conversion chip AD0809 can basically complete the pulse signal collection and processing, the signal amplification is more noticeable, which has A certain role in promoting to reveal the objective.

OBJECTIVE To investigate the genotype distribution in extended-spectrum ?-lactamases-producing Escherichia coli in our hospital.METHODS Clinical strains of E.coli confirmed to produce ESBLs were collected from our hospital.ESBLs genotype was analyzed by plasmid conjugation,PCR and nucleotide sequencing analysis.RESULTS The susceptiblility rate of ESBLs-producing strains were 100% to imipenem,75.4% to piperacillin/tazobactam.A total of 4 genotypes were identified in the 28 ESBLs-producing strains.CTX-M type was identified in 92.9% of the strains,including CTX-M-14,CTX-M-24 and CTX-M-3.SHV Type was identified in 7.1% of the strains,including SHV-12.CONCLUSIONS ESBLs producers are common in E.coli isolated from our hospital.Most of them are multidrug resistant and CTX-M is the main genotype of ESBLs.Other genotypes of ESBLs are not found.

Objective:To discuss the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image.Methods: To separate anatomical structure and function parameter information from Doppler ultrasound medical image, and then combines with the three-dimensional distribution to reconstruct it and fuse imaging.Results: Ultimately, it reveals the relationship between function parameter and anatomical structure. The dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image is also expounded in this paper.Conclusion: It scientifically completed the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image by combining both tissue Doppler imaging and ultrasound medical image reconstruction technique. It is of great significance in clinical medicine.

Objective To study the effect of renal ischemic reperfusion injury on intracellular ionized calcium level and apoptosis. Methods The model of rat's acute renal ischemic reperfusion injury was established.A total of 30 Wistar rats were divided into 5 groups:control, 1 h ischemia and 1 h,2 h,24 h reperfusion groups (n=6 for each group).Renal intracellular [Ca 2+]i level was determined by Fura-2/AM fluorescence assay,and renal cell apoptosis rates were measured by flow cytometry. Results The renal intracellular [Ca 2+]i levels at 1h,2h and 24h timepoint of reperfusion were 156.2 nmol/L,181.6 nmol/L and 260.6 nmol/L,respectively,which were significantly higher (P

OBJECTIVE To study the diagnosis and treatment of parotid acinic cell carcinoma. METHODS The clinical data of 19 cases with parotid acinic cell carcinoma treated from 1985 to 2007 were retrospectively reviewed.Four patients underwent superficial parotidectomy,5 patients underwent total parotidectomy,6 patients underwent superficial parotidectomy plus partial deep parotidectomy,6 patients underwent total parotidectomy and neck dissection.Eight patients received postoperative radiotherapy.One patient only received radical radiotherapy after biopsy.All patients were followed up for 1 to 20 years.RESULTS Nobody died after operation.Five cases recurred after operation, and the recurrence rate was 26.3%(5/19) CONCLUSION The main treatment for parotid acinic cell carcinoma is complete resection and postoperative radiotherapy.

Objective To evaluate the application value of magnetic resonance cholangiopancreatography (MRCP) in the pa‐tients suffering from obstructive jaundice .Methods 126 patients diagnosed as obstructive jaundice underwent the abdominal MRCP examination and MRI scanning .The diagnostic results were recorded and the follow‐up observation was persisted .The MRCP diag‐nosis results were compared with the pathological or ERCP results obtained by follow up .Results Comparing the MRCP diagnostic results with the follow up results ,the coincidence rate of location diagnosis was 100% .The MRCP and follow up results in the qual‐itative diagnosis had no statistically significant difference .M RCP had the higher sensitivity ,specificity ,positive value and negative value in the judgement of different causes of obstruction .Conclusion M RCP has the higher accuracy for the qualitative and location diagnosis of obstructive juandice and also has the higher diagnostic value for judging the different obstructive causes .

Objective To study the effect of drug combined with psychological intervention on mental status and quality of life in patients with multi drug resistant pulmonary tuberculosis.MethodsIn Jiashan Second People's Hospital from January 2011 to December 2016 were 100 cases of multi drug resistant tuberculosis patients as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.Patients in the control group were given routine drug treatment, and the patients in the experimental group were treated with psychological intervention on the basis of drug treatment.Comparison of the experimental group and the control group after 12 months of treatment, depression score and quality of life score.ResultsBefore treatment, there was no significant difference between the experimental group and the control group in the scores of life quality and the scores of depression and anxiety, which was not statistically significant.After treatment in December, the anxiety score of the patients in the experimental group was (41.2±8.2), and the score of depression was (36.4±4.5).The total score of quality of life was (75.2±11.2).The anxiety score of the control group was (52.3±7.4), and the score of depression was (45.4±5.6).The total score of QOL was (64.2±9.3).Thus, after the corresponding treatment, the experimental group of patients with depression and anxiety scores were significantly lower than those in the control group, the quality of life scores of the control group was significantly lower than the experimental group, with statistical difference (P<0.05).ConclusionThe drug combined with psychological intervention in treatment of multi drug resistant pulmonary tuberculosis can improve the therapeutic effect to a great extent, help the patients to relieve anxiety and depression and other negative emotions, improve the quality of life, with further clinical promotion and application significance.

Objective:To study the expression and clinical relevance of IRAK-M in monocytes in patients with systemic lupus erythematosus. Methods: Real-time quantitative PCR was performed for IRAK-M mRNA measurement and enzyme-linked immunosorbent assay was used for anti-double-stranded DNA antibody ( dsDNA ) and anti-single-stranded DNA antibody ( ssDNA ) . Dynamic scattering turbidimetric immunoassay was applied for complement 3(C3),complement 4(C4) and C-reactive protein(CRP), while Westergren method for erythrocyte sedimentation rate (ESR). Correlation analysis of IRAK-M with SLEDAI,dsDNA,ssDNA,C3, C4,CRP and ESR was computed by Pearson or Spearman. Results:①The result showed that the mRNA expression of IRAK-M in SLE patients was significantly lower than healthy controls (P<0. 05);and the mRNA expression of IRAK-M in active group was significantly lower than stable group ( P<0. 05 ) . ②The levels of dsDNA, ssDNA, CRP and ESR in SLE patients were significantly higher than healthy controls( P<0. 05 );and the levels of C3 and C4 in SLE patients were significantly lower than healthy controls ( P<0. 05 ) . Meanwhile,the levels of dsDNA,ssDNA,CRP and ESR in active group were significantly higher than stable group (P<0. 05),and the levels of C3 and C4 in active group were significantly lower than stable group (P<0. 05).③Correlation analysis showed that the mRNA expression of IRAK-M had a positive correlation with C3 (P<0. 05),and had a negative correlation with SLEDAI,dsDNA,CRP and ESR (P<0. 05),but had no correlation with ssDNA and C4(P>0. 05). Conclusion: This study indicated that IRAK-M play certain significant roles in the pathogenesis of SLE. We can monitor SLE disease activity and prognosis by quantitative detection of mRNA expression of IRAK-M. Meanwhile,it is very necessary to routinely test and regularly monitor the levels of dsDNA,ssDNA,C3,C4,CRP and ESR in SLE.

Objective To study the phase transformations and chemical composition changes of Pyritum Preparata (PP) influenced by different processing. Methods To analyse the changes of microstructure, morphology, mineral phase, and chemical compositions in different PP by ore microscope, electron probe microanalyzer technique. Results Greater changes have taken place in chemical compositions of PP after calcination: pyrite (FeS_2) changed into pyrrhotite (Fe_(1-x)S), even changed into magnetite (Fe_3O_4); and chalcopyrite generated mutation to be low-copper chalcopyrite, the contents of Cu reduced, the rate of Cu/Fe changed. At the same time, in the pyrrhotite around the mutative chalcopyrite, the contents of Cu increased. That is to say, the Cu got into pyrrhotite and generated solid-solution with high dispersion, and then ore became loose and porous. In the hard-burned spot of calcined quenched samples by vinegar, the contents of Pb and S are far lower than non-burned spot, the minimum estimate of Pb is less than 1/10 of crude ore. Conclusion There is a co-relativity between phase transformations and dissolution of chemical compositions before and after calcining PP.