OBJECTIVE:To probe into effect of atorvastatin on Intima-media thickness and endothelial function.METHODS:60 patients with mild and moderate angiostegnosis of internal carotid were randomized into control group (n=30,0.1 g aspirin enteric-coated tablet) and experiment group (n=30,0.1 g aspirin enteric-coated tablet and 20 mg atrovastatin).Intima-media thickness and endothelial function were compared in 3 and 6 months before vs.after treatment.RESULTS:As compared with before treatment,intima-media thickness and endothelial function in experiment group were improved significantly after treatment (P0.05).CONCLUSION:Atorvastatin shows good effect on,improvement of intima-media thickness endothelial function,stabilization of atheromatous plaque,delay and prevention of angiostegnosis.

BACKGROUND: Intravascular stent implantation is one of the main methods for intra-extracranial artery stenosis. The safety and efficacy of stent implantation has been elevated, and symptoms or ischemic advert events with artery stenosis has been reduced. However, risks of thrombosis, cerebralhemorrhage, restenosis, and hyperperfusion still existed. OBJECTIVE: To investigate the influence of atorvastatin on ischemic adverse events following cerebral middle artery stent implantation.METHODS: Twenty-four patients received cerebral middle artery stent implantation were randomly divided into 2 groups. In the treatment group, patients were received atorvastatin 40 mg + plavix 75 mg + aspirin enteric-coated tablet 300 mg, once per day; in the control group, patients were received plavix 75 mg + aspirin enteric-coated tablet 300 rag, once per day. Ischemic adverse events, such as in-stent restenosis, transient ischemic attack, cerebral infarction, or re-interventional therapy were observed by transcranial Doppler, digital subtraction angiography, nuclear magnetic resonance imaging, diffusion weighted imaging, blood lipid level and C-reactive protein level examinations prior to and at months 1, 3, 6 and 12 after stent implantation.RESULTS AND CONCLUSION: Compared with the control group, the levels of blood lipid and C-reactive protein were obviously decreased at months 1, 3, 6 and 12 after treatment (P<0.05, P<0.01 ), with dramatically declined ischemic adverse events (P<0.05, P<0.01 ). Compared with before operation, the levels of blood lipid and C-reactive protein were decreased after operation in the treatment group (P<0.05, P<0.01). No server adverse events occurred in patients treated by atorvastatin, which showed 40 mg was safe for patients. The results revealed that atorvastatin combined with antiplatelet therapy can prevent ischemic adverse events following cerebral middle artery stent implantation.

Objective To evaluate the application value of magnetic resonance cholangiopancreatography (MRCP) in the pa‐tients suffering from obstructive jaundice .Methods 126 patients diagnosed as obstructive jaundice underwent the abdominal MRCP examination and MRI scanning .The diagnostic results were recorded and the follow‐up observation was persisted .The MRCP diag‐nosis results were compared with the pathological or ERCP results obtained by follow up .Results Comparing the MRCP diagnostic results with the follow up results ,the coincidence rate of location diagnosis was 100% .The MRCP and follow up results in the qual‐itative diagnosis had no statistically significant difference .M RCP had the higher sensitivity ,specificity ,positive value and negative value in the judgement of different causes of obstruction .Conclusion M RCP has the higher accuracy for the qualitative and location diagnosis of obstructive juandice and also has the higher diagnostic value for judging the different obstructive causes .

Objective:To discuss the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image.Methods: To separate anatomical structure and function parameter information from Doppler ultrasound medical image, and then combines with the three-dimensional distribution to reconstruct it and fuse imaging.Results: Ultimately, it reveals the relationship between function parameter and anatomical structure. The dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image is also expounded in this paper.Conclusion: It scientifically completed the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image by combining both tissue Doppler imaging and ultrasound medical image reconstruction technique. It is of great significance in clinical medicine.

Objective:To study the expression and clinical relevance of IRAK-M in monocytes in patients with systemic lupus erythematosus. Methods: Real-time quantitative PCR was performed for IRAK-M mRNA measurement and enzyme-linked immunosorbent assay was used for anti-double-stranded DNA antibody ( dsDNA ) and anti-single-stranded DNA antibody ( ssDNA ) . Dynamic scattering turbidimetric immunoassay was applied for complement 3(C3),complement 4(C4) and C-reactive protein(CRP), while Westergren method for erythrocyte sedimentation rate (ESR). Correlation analysis of IRAK-M with SLEDAI,dsDNA,ssDNA,C3, C4,CRP and ESR was computed by Pearson or Spearman. Results:①The result showed that the mRNA expression of IRAK-M in SLE patients was significantly lower than healthy controls (P<0. 05);and the mRNA expression of IRAK-M in active group was significantly lower than stable group ( P<0. 05 ) . ②The levels of dsDNA, ssDNA, CRP and ESR in SLE patients were significantly higher than healthy controls( P<0. 05 );and the levels of C3 and C4 in SLE patients were significantly lower than healthy controls ( P<0. 05 ) . Meanwhile,the levels of dsDNA,ssDNA,CRP and ESR in active group were significantly higher than stable group (P<0. 05),and the levels of C3 and C4 in active group were significantly lower than stable group (P<0. 05).③Correlation analysis showed that the mRNA expression of IRAK-M had a positive correlation with C3 (P<0. 05),and had a negative correlation with SLEDAI,dsDNA,CRP and ESR (P<0. 05),but had no correlation with ssDNA and C4(P>0. 05). Conclusion: This study indicated that IRAK-M play certain significant roles in the pathogenesis of SLE. We can monitor SLE disease activity and prognosis by quantitative detection of mRNA expression of IRAK-M. Meanwhile,it is very necessary to routinely test and regularly monitor the levels of dsDNA,ssDNA,C3,C4,CRP and ESR in SLE.

Objective To explore a new project-integrated teaching method and evaluate its effect in the course of optometry special examination techniques. Methods Class Three of Optometry 2011 was chosen as experimental group and used the new project integrated teaching method while Class Four was chosen as control group and used traditional teaching method , both class having 30 students. After the end of the course the students of both class were assessed the theoretical knowledge and operating skills of the same standard. The self-designed questionnaire was used to investigate stu-dents' self evaluation of the teaching effect. After students of both groups practiced in the same depart-ment of ophthalmology optometry clinic special inspection post for half a year, teachers' evaluation of the students' training performance was collected. Final examination scores, experimental examination scores and the teachers' evaluation scores of the two groups were compared by t-test respectively and the self-assessment results of the students of both groups were compared by chi-square test. Results The final examination and experimental examination scores of the experimental group were both higher than those of the control group significantly(P=0.001,0.002 respectively). The experimental group students had a significantly better self-assessment than the control group in learning interest ( P=0.007), understanding(P=0.046), self-learning ability(P=0.000), summary ability(P=0.009), ana-lytical ability(P=0.000) and innovation ability(P=0.000). The clinical teachers' evaluations of the ex-perimental group were better than the control group. The differences were statistically significant in standardization of practice, application ability, examination report reading ability and case analytical ability(P=0.000). Conclusion The project-integrated teaching method can help to improve the students' leaning motivation and cultivate students' autonomous learning ability and comprehensive analysis capa-bility and so on, which is helpful to reaching a good teaching effect and ensuring the teaching quality.

Objective To study the phase transformations and chemical composition changes of Pyritum Preparata (PP) influenced by different processing. Methods To analyse the changes of microstructure, morphology, mineral phase, and chemical compositions in different PP by ore microscope, electron probe microanalyzer technique. Results Greater changes have taken place in chemical compositions of PP after calcination: pyrite (FeS_2) changed into pyrrhotite (Fe_(1-x)S), even changed into magnetite (Fe_3O_4); and chalcopyrite generated mutation to be low-copper chalcopyrite, the contents of Cu reduced, the rate of Cu/Fe changed. At the same time, in the pyrrhotite around the mutative chalcopyrite, the contents of Cu increased. That is to say, the Cu got into pyrrhotite and generated solid-solution with high dispersion, and then ore became loose and porous. In the hard-burned spot of calcined quenched samples by vinegar, the contents of Pb and S are far lower than non-burned spot, the minimum estimate of Pb is less than 1/10 of crude ore. Conclusion There is a co-relativity between phase transformations and dissolution of chemical compositions before and after calcining PP.

Objective To evaluate the complementary value of 11C-choline (CHO) PET/CT to 18F-FDG PET/CT in the staging of locally advanced nasopharyngeal carcinoma (NPC) and diagnosis of HCC.Methods From December 2007 to January 2010,15 patients with locally advanced NPC and 76 patients with HCC were prospectively enrolled into this study.The research was approved by the ethics committee,and all patients signed informed consents.Whole body 18 F-FDG PET/CT scans were performed on all patients and regional 11C-CHO PET/CT was conducted in 43 patients (15 with NPC,28 with HCC).A lesion with increased uptake of either 11 C-CHO or 18F-FDG was considered positive.SUVmax,tumor/brain (T/B) ratio and tumor/liver (T/L) ratio were calculated for semi-quantitative analysis.Two-sample t test,x2 test,Fisher exact test and linear correlation analysis were used for statistical analysis.Results (1) The lesion SUVmax of 18 F-FDG was higher compared to 11C-CHO (12.81 ± 5.00 vs 6.84 ± 2.76 ; t =6.416,P ＜0.01) in NPC patients.However,11C-CHO PET/CT had a much higher T/B ratio than 18F-FDG (18.62 ±7.95 vs 1.38 ±0.59 ; t =8.801,P ＜ 0.01).Significant correlation was found between the 2 tracers with regard to NPC lesion uptake (r =0.712,P ＜0.01).Compared with 18F-FDG PET/CT,11C-CHO PET/CT had better delineation of intracranial invasion in 50.0％ of patients (12/12 vs 6/12; x2 =8.000,P ＜0.05),skull base invasion in 4/14 patients and orbital invasion in 3/3 patients.(2) 18F-FDG PET/CT showed positive findings in 63.1％ (48/76) of HCC patients.In 28 HCC patients with negative findings on 18F-FDG PET/CT,11 C-CHO PET/CT was positive in 71.4％ (20/28) of patients.The dual-tracer PET/CT improved the diagnostic sensitivity (89.5％,68/76) of HCC compared with 18F-FDG PET/CT (63.1％,48/76) alone (x2 =14.559,P ＜0.01).11C-CHO PET/CT was more sensitive than 18F-FDG PET/CT for the detection of well differentiated HCC (6/9 vs 35.7％ (5/14) ; P =0.214).For the detection of moderately differentiated HCC,the sensitivity of 11C-CHO and 18F-FDG PET/CT was similar to each other (6/7vs 72.0％ (18/25),P =0.648).11C-CHO PET/CT was more sensitive than 18F-FDG for the detection of HCC lesions ＜5.0 cm (72.7％ (16/22) vs 42.1％ (16/38) ; x2 =5.249,P ＜0.05),especially for lesions ＜ 2.0 cm (5/7 vs 0/7; P =0.021).Conclusions 11 C-CHO PET/CT could improve the accuracy in T staging of NPC.It might also play a complementary role for 18 F-FDG PET/CT in the detection of HCC.

Objective To investigate the effect of radix paeoniae rubra (RPR) on expressions of p38MAPK, NF-κB and iNOS in ltpopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats with ALI were divided randomly into five groups: saline control group (Group A) , LPS group (Group B), RPR for treatment group (Group C), RPR for prevention group (Group D) and SB203580 group (Group E). The effects of RPR on protein content and the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), contents of malondidehyde (MDA) and serum NO in lung tissue were observed. Arterial blood was collected for blood gas analysis. Expressions of p38MAPK, NF-κB and iNOS in rat lung tissues were detected. Results Compared with Group A, expressions of p38MAPK, NF-κB and iNOS were significantly increased (P <0.01 orP< 0.05) , the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly higher (P<0.01 or P<0.05) in Groups B, C, D and E. There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in Groups B, C, D and E (P<0.01 or P<0.05). Compared with Group B, the expressions of p38MAPK, NF-κB and iNOS were significantly lower, and the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly decreased, while the levels of arterial bicarbonate and partial pressure of oxygen were significantly higher in Groups C, D and E (P<0.05 or P<0.01). Compared with Group B, the lunginjury of Groups C, D and E was significantly alleviated, while there was no statistical difference among Groups C, D and E in the indices of lung injury. Conclusion Protective effect of RPR on LPS-induced ALI is closely related to the inhibited expressions of p38MAPK, NF-κB and iNOS.

Objective: Combined detection of anti-mutated citrullinated vimentin ( anti-MCV ) antibodies, anti-cyclic citrullinated peptide ( anti-CCP) antibodies and rheumatoid factor ( RF-IgM) levels to investigate the diagnostic value of anti-mutated citrullinated vimentin (anti-MCV) antibodies for rheumatoid arthritis(RA).Methods: A total of 359 patients with RA,128 patients with other rheumatic diseases and 90 healthy controls were involved.Enzyme linked immunosorbent assay ( ELISA) was used to detect anti-MCV and anti-CCP, and dynamic immune nephelometry was applied to detect RF-IgM .The sensitivity and specificity were obtained from the experimental data.Results:The sensitivities of anti-MCV,anti-CCP and RF-IgM were 85.1%,76.7% and 82.7%in RA respectively.The specificities were 93.2%,95.1%and 80.1%respectively.Combined detection of anti-MCV and anti-CCP,the sensitivity decreased to 70.2%; but the specificity increased to 98.7%.The sensitivity reached to 89.5% with specificity 97.6%when the union of anti-MCV and anti-CCP positivity was used as criterion.Conclusion:Anti-MCV and anti-CCP are novel makers for RA diagnosis with high sensitivity and high specificity.Combination of anti-MCV and anti-CCP is more helpful for RA diagnosis.