OBJECTIVE: This study was designed to find out the impact of micro-ecological environment on the incidence of allergic rhinitis after developing a model of allergic rhinitis on mice. METHOD: Sixty mice were randomly divided into GF group (n=30) and SPF group (n=30). Mice of GF group were fed in the germ-free environment and mice of SPF group were fed in the specific pathogen-free environment. Then each group were randomly divided into model group (20 mice) and control group (10 mice). Establish allergic rhinitis model in the mice of model group using ovalbumin (OVA) at the age of 6 weeks, observe and score the corresponding symptoms and signs that could been induced. Stain with hematoxylin eosin (HE) staining method for nasal mucosa to observe the morphological changes. Using enzyme linked immunosorbent assay to detect the concentration of IgE, IFN-γ and IL-4 in the peripheral blood serum. RESULT: The chi square test showed that the incidence of allergic rhinithis in the mice of GF group was significantly higher than that in the SPF group (P< 0. 05). HE staining showed that the nasal mucosas of allergic rhinitis positive reaction mice were highly congestive and edematous and had a large number of inflammatory cell infiltration, while there was no abnormal morphology of nasal mucosas in mice with no allergic rhinitis reaction. EOS counting displayed that the number of eosinophilic cells in nasal mucosa of positive allergic rhinitis reaction mice was increased significantly. The concentration of IgE and IL-4 in the serum of positive allergic rhinitis reaction mice was highly increased (P <0. 05), and IFN-γ was significantly decreased (P< 0.05). CONCLUSION The difference of micro-ecological environment may play a key role in the occurrence of allergic rhinitis in mice.
Animals ; Disease Models, Animal ; Environment ; Incidence ; Interleukin-4 ; Mice ; Mice, Inbred BALB C ; Nasal Mucosa ; Ovalbumin ; Rhinitis ; Rhinitis, Allergic ; etiology

Objective:To explore the effect of the postoperative rehabilitation model based on the hierarchy of needs theory in patients with ovarian cysts.Methods:From February 2018 to July 2020, convenience sampling was used to select 151 patients with ovarian cysts in Fuyang Cancer Hospital as the research object. The patients were divided into the control group ( n=72) and the observation group ( n=79) by the random number table. The control group conducted routine postoperative nursing, and the observation group implemented postoperative rehabilitation model based on the hierarchy of needs theory. The two groups of patients were compared in terms of getting out of bed, exhausting gas, defecation, hospital stay, and scores of the Life Events Scale (LES) , Frankl Compliance Scale (FCS) and Exercise of Self-Care Agency Scale (ESCA) before and after the intervention. Results:The time of getting out of bed, exhausting gas, defecation and hospital stay in the observation group were shorter than that in the control group, and the differences were statistically significant ( P<0.01) . After intervention, the LES score of the observation group was lower than that of the control group, and the FCS score was higher than that of the control group, with statistical differences ( P<0.01) . The ESCA scores of self-concept, health knowledge, self-care skills and self-care responsibility in the observation group were higher than those in the control group after intervention, and the differences were statistically significant ( P<0.01) . Conclusions:The postoperative rehabilitation model based on the hierarchy of needs theory helps to speed up the physical recovery of ovarian cyst patients, relieve mental stress, and improve treatment compliance and self-care ability.

Objective:To explore the application effect of cognitive-behavioral pathway intervention based on empowerment theory in patients with cervical cancer surgery.Methods:Using the convenient sampling method, a total of 70 patients with cervical cancer who underwent surgical treatment in Fuyang Cancer Hospital from January 2019 to December 2021 were selected as the research objects. According to the time of admission, they were divided into the control group and the experimental group, with 35 cases in each group. The control group was given routine nursing intervention, while the experimental group was given cognitive-behavioral pathway intervention based on empowerment theory on the basis of the control group. The scores of Self-care Ability Assessment (ESCA) , Baker Anxiety Inventory (BAI) , Baker Depression InventoryⅡ (BDI-Ⅱ) and Quality of Life were compared between the two groups before the intervention and after 4 weeks of intervention.Results:After 4 weeks of intervention, the total score of ESCA and scores of health knowledge, self-concept, self-responsibility and self-care skills in the experimental group were higher than those in the control group [ (93.62±18.19) vs. (83.87±18.30) , (34.26±6.23) vs. (31.26±5.61) , (18.25±5.34) vs. (15.34±4.26) , (18.24±4.28) vs. (16.04±3.78) , (22.87±2.34) vs. (21.23±4.65) points, all P<0.05]. The BAI score and BDI-Ⅱ score of the experimental group were lower than those of the control group [ (42.29±3.59) vs. (45.26±4.65) , (12.67±3.48) vs. (14.26±4.15) points, all P<0.05]. In the Quality of Life scale of the experimental group, the scores of disease concerns, activity limitation, mood and energy, life out of control, self-focus and sexual function were all higher than those of the control group [ (19.94±4.89) vs. (17.34±4.22) , (30.11±5.13) vs. (27.49±4.22) , (28.49±5.16) vs. (25.48±4.87) , (17.74±4.88) vs. (16.34±4.25) , (11.65±3.95) vs. (10.34±3.45) , (7.69±3.15) vs. (5.68±2.89) points, all P<0.05]. Conclusions:The cognitive-behavioral pathway intervention based on the empowerment theory for patients with cervical cancer surgery can improve the self-care ability of patients, reduce anxiety, depression and traumatic stress symptoms, promote rehabilitation and improve the quality of life.

Objective To assess the outcomes of ischemic stroke patients with atrial fibrillation (AF).Methods Six thousand six hundred and ninety-five patients with acute ischemic stroke,admitted to our hospital from May 2005 to December 2013,were recruited consecutively.These patients were divided into combined AF group (n=583) and non-combined AF group (n=6112).The clinical data,including stroke subtypes,stroke severity,risk factors of stroke,NIHSS scores and Barthel index,and outcomes,including mortality,unfavourable prognosis and recurrence at 3,12,and 36 months after stroke were analyzed.Results The prevalence rate of AF in the patients enrolled in this study was 8.7％ (583/6695).There was a higher frequency of AF in female than that in male,with significant difference (45.8％ vs.33.0％,P＜0.05).The patients from combined AF group were older than those without AF.The percentage of severe stroke in AF patients (34.8％) was significantly higher than that in non-combined AF group (8.3％,P＜0.05).The patients with AF were less likely than the patients without AF to have hypertension (63.8％ vs.73.3％),diabetes (24.9％ vs.32.3％),dyslipidemias (26.1％ vs.31.5％),artery stenosis (17.2％ vs.23.4％),current smoking (22.6％ vs.39.0％),and alcohol consumption (7.5 ％ vs.18.9％),with significant differences (P＜0.05).After adjusting age,gender,stroke subtype,and severity,and risk factors,multivariate analysis showed that there was a higher recurrence risk in combined AF group at 3 months after stroke than that in non-combined AF group (P＜0.05);patients with AF had significantly higher mortality,dependency,and recurrence rates at 12 and 36 months after stroke than those without AF (P＜0.05).Conclusion The long-term prognosis of patients with stroke complicated with AF is poor;therefore,normalized anticoagulant therapy should be taken to decrease the recurrence rate and burdens of stroke in China.

Objective:To explore the potential categories and influencing factors of online learning behavior in Junior college nursing students in post epidemic era.Methods:A total of 978 nursing students from Heilongjiang Nursing College in December 2021 were investigated by the general information questionnaire, Online Learning Behavior Scale, Online Learning Self-Efficacy Scale, Proactive Personality Scale and Online Learning Attitude Scale. The latent profile analysis and ordered multi-classification Logistic regression analyses were applied to identify the factors associated with the potential categories of online learning behavior.Results:The online learning behavior of nursing students were divided into 3 categories: outstanding(12.68%, 124/978), good(75.66%,740/978)and mediocrity(11.66%, 114/978). Gender, online learning self-efficacy, proactive personality and online learning attitude were predictors that influence the classification of potential profiles of online learning behavior in nursing students( P<0.05). Conclusions:There are 3 potential categories of online learning behavior in nursing students, and nursing educators should pay attention to the characteristics of online learning behaviors of different nursing students and take various effective measures to improve online learning behaviors of nursing students.

Objective To investigate the clinical application value of wrist joint ulnar deviation supination 45° palmar oblique posi-tion in the diagnosis of scaphoid waist fracture and displacement.Methods The imaging and clinical data such as digital radiography(DR),CT of 93 wrist joint trauma patients were analyzed.The four position views including wrist joint anteroposterior+lateral view,scaphoid position,wrist joint ulnar deviation supination 45° palmar oblique position,scaphoid position+wrist joint ulnar devia-tion supination 45° palmar oblique position were analyzed by three readers.The consistency of the evaluation among different readers and the diagnostic efficiency of the diagnosis of scaphoid fracture and displacement were compared.Results The inter-observer agreement,sensitivity,specificity,accuracy,and other diagnostic efficiency of scaphoid waist fracture and displacement was evaluated,wrist joint ulnar deviation supination 45° palmar oblique position+scaphoid position and wrist joint ulnar deviation supination 45° palmar oblique position were better than those of scaphoid position and wrist joint anteroposterior+lateral view.The combination of wrist joint ulnar deviation supination 45° palmar oblique position+scaphoid position obtained the best diagnostic efficiency.Conclusion The wrist joint ulnar deviation supination 45° palmar oblique position shows the long axis of the scaphoid,which has a high diagnostic efficiency in the diagnosis of scaphoid waist fracture and displacement and would be used as a useful supplement to other scaphoid imaging.

Objective To explore the effect of thymic stromal lymphopoietin (TSLP) on transformation of dendritic cell (DC) and T cell in vitro.Methods Mouse-derived immature dendritic cells and T lymphocytes were co-cultured in vitro,which were divided into 4 groups (TSLP stimulation group,TSLP stimulation and its receptor blocking group,ovalbumin stimulation group and ovalbumin stimulation and TSLP receptor blocking group).IL-4,IL-8 and IFN-β in cell culture supernatant were detected after 2 days by ELISA.SPSS 13.0 software was used to analyze the data.Results IL-4 levels of TSLP receptor blocking groups [(48.84 ± 1.56) pg/ml,(52.53 ± 2.36) pg/ml] were significantly lower than those of corresponding TSLP stimulation group and ovalbumin stimulation group [(72.55 ± 7.76) pg/ml,(80.47 ± 21.93) pg/ml ; t =5.994,P ＜ 0.05 ; t =2.534,P ＜ 0.05].However,there were not significant differences of IL-8 and IFN-β expression between corresponding two groups of whether or not TSLP receptor blocking (all P ＞ 0.05).Conclusion TSLP receptor blockade in vitro can inhibit T lymphocyte transformation to Th2,which may provide a new therapeutic strategy for clinical Th2 dominant diseases such as allergic rhinitis and asthma.

Objective:To investigate the regulatory role of defferentially expressed LOC107987438 in the pathogenesis of depressive disorder and provide a theoretical basis for its clinical application in depressive disorder.Methods:Differential expression of LOC107987438 was verified by quantitative real-time polymerase chain reaction(qRT-PCR)in peripheral blood monocular cells(PBMCs)of 60 patients with depressive disorder and 60 health controls. In addition, its diagnostic value was assessed by receiver operating characteristic(ROC)curves. Based on the ceRNA mechanism of lncRNA, the miRDB database was applied to predict the target miRNAs of LOC107987438, and the miRNAs with target score ≥ 80 among them were screened out.The screened miRNAs were then used to predict their potential target mRNAs through four databases which were TargetScan 8.0, miRTarBase, mirDIP and miRPathDB. Moreover, the predicted target mRNAs were annotated for gene ontology(GO)function annotation and tokoyo encyclopedia of genes and genomes(KEGG) pathway enrichment analysis via ClusterProfiler 4.0.5 package of R 4.1.1. Finally, a protein-protein interaction network was constructed using the STRING 11.5 platform to retrieve the interacting genes.Results:The qRT-PCR results showed that normalized expression of LOC107987438 in PBMCs of patients with depressive disorder was higher than that in health controls(depressive disorder: 2.084±1.357, health controls: 1.000±0.660, P<0.001). The ROC curve results showed that the area under curves(AUC)of LOC107987438 was 0.759(95% CI: 0.675-0.842, P<0.05), indicating its high potential diagnostic value. Bioinformatics analysis showed that hsa-miR-4670-3p, hsa-miR-619-3p, hsa-miR-6721-5p and hsa-miR-297 were the miRNAs with high bindings to LOC107987438. The results of KEGG signaling pathway enrichment revealed that hypoxia-inducible factor 1(HIF-1)signaling pathway, phosphatidylinositol 3-kinase-AKT(PI3K-Akt) signaling pathway and erythroblastic oncogene B(ErbB) signaling pathway were closely associated with depressive disorder. Among the top ten key genes screened by the protein-protein interaction network, kirsten rats arcomaviral oncogene homolog(KRAS), androgen receptor(AR), cyclic-AMP response binding protein1(CREB1), insulin-like growth factor 1(IGF1), cyclin-dependent kinase inhibitor 1B(CDKN1B) and calcium/calmodulin-dependent protein kinase type Ⅱ alpha(CAMK2A)were strongly associated with depressive disorder. Conclusion:The establishment of ceRNA regulatory network of LOC107987438 provides a theoretical basis for exploring the pathophysiology of depressive disorders.

Objective To compare the clinical efficacy and short-term prognosis of laparoscopic radical resection of right colon cancer guided by superior mesenteric artery and superior mesenteric vein.Methods 80 patients with right colon cancer of cT2-4 and/or N0-2M0 admitted from January 2020 to October 2022 were selected as the research objects,and they were randomly divided into observation group and control group,with 40 patients in each group.The observation group was treated with SMA-oriented laparoscopic radical resection of right colon cancer,while the control group was treated with SMV-oriented laparoscopic radical resection of right colon cancer.The curative effect and prognosis of the two groups were compared.Results There was no significant difference between the two groups in general condition,operation time,gastric tube placement time,recovery time of farting,postoperative fasting time,postoperative drainage time,postoperative nutritional index,total incidence of complications and postoperative hospitalization time(P>0.05).The lymph nodes in the observation group were significantly more than those in the control group,and the difference was statistically significant(P<0.05).In the observation group,the lymph nodes in the anterior and left side of superior mesenteric artery were examined(No.D3),and 273 lymph nodes were detected,and Seven patients(17.5% )were diagnosed with D3 metastasis,and 13 lymph nodes were positive(5.2% ).Conclusion Laparoscopic radical resection of right colon cancer guided by superior mesenteric artery,without increasing the incidence of complications and high safety,can more thoroughly clean lymph nodes and reduce tumor recurrence,which is expected to significantly improve the prognosis of patients.