Computer programming courses is generally considered as the most difficult courses to understand and study.We have carried out some reforms in teaching experiment:making learning objective clearer to stimulate students' learning desire;adjusting teaching content to enhance the students' learning interest;combining teachers'accessible elucidation with students' operation in the class to improve both the teaching effect and students' comprehensive application capability.

Objective To observe conditioned enhancement of antibody production against influenza vaccine. Methods 36 female BALB/c mice were injected with 3 μg/mouse influenza virus hemagglutinin (HA) as the unconditioned stimulus (UCS),and camphor odor was served as the conditioned stimulus (CS). After a CS/UCS pairing was made,animals were re-exposed to the CS at Weeks 6. Results Through one conditioned stimulus,the optical density of anti-HA antibody of the conditioned group (Weeks 9:0.68±0.06; Weeks 10:0.60±0.06)was significantly increased compared with the unconditioned group (Weeks 9:0.53±0.06; Weeks 10:0.48±0.04) ( P <0.01). The level of anti-HA antibody of the conditioned group was also significantly greater than other controlled groups( P <0.05). Conclusion Through a single exposure to camphor odor which was paired with immunization of influenza virus HA in a single trial learning protocol,a significant conditioned anti-HA IgG production occurred.

Objective To investigate the effects of pretreatment with remifentanil on hemorrhagic shock (HS)-induced acute liver injury in rabbits.Methods Thirty-two New Zealand white rabbits,weighing 2.0-2.5 kg,were randomly divided into 4 groups (n=8 each): sham operation group (group S) ; group HS,low-dose remifentanil group (group RL) ; high-dose remifentanil group (group RH).Remifentanil was infused at 0.66 and 1.32 μg· kg-1 · min-1 for 145 min in groups RL and RH respectively,while the equal volume of normal saline was infused in group C.HS was induced by withdrawing blood from the left femoral artery at 15 min after continuous infusion of normal saline or remifentanil and mean arterial pressure (MAP) was reduced to 40 mm Hg.MAP was reduced to 35-45 mm Hg and maintained at this level for 120 min in groups HS,RL and RH.Blood samples were taken for determination of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities before administration (T0),immediately before blood letting (T1),and at 10,30,60 and 90 min after blood letting (T3-6).The animals were then sacrificed and the livers were immediately removed for microscopic examination.Results Compared with group S,the serum ALT activity at T5 and T6 and serum AST activity at T4-T6 were significantly increased in the other three groups (P ＜ 0.05).Compared with group HS,the serum ALT activity at T5 and T6,and serum AST activity at T4-T6 were significantly decreased in group RH,and no significant change was found in group RL (P ＞ 0.05).Compared with group RL,the serum ALT activity at T5 and T6,and serum AST activity at T4-T6 were significantly decreased in group RH (P ＜ 0.05).The serum ALT activity at T5 and T6 and serum AST activity at T4-T6 were significantly increased in groups HS,R and RH than those at T0 (P ＜ 0.05 or 0.01).The patho1ogical injury was attenuated in group RH compared with groups HS and RL.Conclusion Remifentanil pretreatment can attenuate HS-induced acute liver injury in rabbits,and the effect is related to the dose.

Objective To develop a new quasi-zero stiffness isolator for the vibration isolation of the sick and wounded on the ambulance stretcher.Methods The isolator was designed and verified on the basis of the quasi-zero stiffness of slotted disk spring, vibration isolation design method with high static while low dynamic stiffness and co-laminated structure of the spring.Results The theoretical analyses and tests proved the force-displacement curves of the new quasi-zero stiffness isolator fit well. The frequency-weighted acceleration at the buttock and head under different speeds on the asphalt roads was less than 0.315 m/s2.Conclusion The new quasi-zero stiffness isolator developed behaves well in vibration isolation and comfortability for the ambulance stretcher sick and wounded.

Objective: To explore the application value of pulmonary ultrasound for the treatment of severe acute respiratory distress syndrome in children with extracorporeal membrane oxygenation(ECMO). Methods: Seven children with severe acute respiratory distress syndrome (ARDS) who were treated with ECMO in the PICU ward of Hunan Children Hospital from August 2018 to March 2019 underwent pulmonary ultrasound before treated with ECMO, after each bronchoscopy and lavage, before ECMO withdrawal, and within 24 hours after ECMO withdrawal. Performance of pulmonary ultrasound and clinical data were retrospectively reviewed. Results: Seven patients treated with ECMO for 11.5(3-27)days, and 1 patient underwent ECMO transport. Six (86.7%) patients were successfully weaned from ECMO, and 1 (14.2%) patient failed to exit ECMO successfully. Four(57.1%) patients were discharged from hospital, and 3(42.8%) patients eventually died. In various diagnostic signs of pulmonary ultrasound were detected in 7 patients, including pulmonary edema (7 cases), lung consolidation (5 cases), followed by pleural effusion (4 cases), atelectasis (3 cases) and pneumothorax (3 cases). Pulmonary ultrasound showed that the lungs were re-expanded and suggested that the child could attempt to evacuate the ECMO. Conclusions Pulmonary ultrasound can comprehensively evaluate the various lung lesions in children with ECMO treatment, and can dynamically monitor the process of lung recruitment in real time, providing a reference for guiding the evacuation of ECMO.

Objective To explore the effects of different processing parameters of mobile digital radiography (DR) on image quality of bedside chest radiography.Methods Post processing parameter on 150 cases bedside DR chest image like multi frequency laplasse transform (MLT),image processing (IP) were modified,and the optimization scheme of post-processing parameters were analyzed and summarized.The paired sample t test was used to compare the difference of the imaging quality score and optical density between the default parameter group and the post processing parameter optimization group through measure the optical density of lung related area.Results Brightness,contrast and the low density in dynamic range were the main factors affecting the quality of bedside chest radiography.After modifying and optimizing the post-processing parameters,the density value of the second intercostal area was 1.68±0.04,the density value of the hilum was 0.77±0.46,the density value of pulmonary peripheral (near chest wall 1 cm and overlap with a single rib) was 0.62± 0.06,the density value of cardiac shadow was 0.44 ± 0.04,the density value of subphrenic was 0.37 ± 0.04,the lung markings was clearly showed in the lateral field of lung and was visible in the spine and heart shadow area which meet the diagnostic requirement.The density value of those parts before modify were lower than the modify value which was more closer to the reference density value,and the difference had statistically significant.The image quality score of the postprocessing parameter optimization group was 3.53 ± 0.14,which was higher than 3.02 ± 0.23 of the default parameter group,and the difference had statistically significant.Conclusion The appropriate post-processing parameters is the key to guarantee the reasonable density of lung areas image quality and the optimization post-processing parameter can improve the image quality of bedside chest radiography by the mobile DR.

Objective To investigate the filtration roles of microporous membranes with different pore sizes in the tumor cells with different diameters,and effects on the filtered cells.Methods Three kinds of tumor cells with different cell diameters and same concentrations,including Jurkat,K562 and A549,were filtered by the polycarbonate microporous membranes with different pore sizes such as 1,3,5,8 and 10 μm,respectively,and their filtration rates were determined.The diameters of three kinds of tumor cells before and after filtration,and the fixed K562 cells with formaldehyde,were measured by an optical microscope.The activity of the filtered K562 cells were detected by the trypan blue staining.After the filtered K562 cells were re-cultured,their proliferation activity was analyzed by the growth curve.Results Jurkat,K562 and A549 cells couldn't pass the filter membrane with 1 μm of pore size.The filtration rates of three kinds of tumor cells passing the fliter membranes with 3 μm,5 μm,8 μm and 10 μm of pore sizes increased in turn.The survival rate of K562 cells filtered by 3 μm of pore size of membrane was 92.0％,and the proliferation acticity of re-cultured K562 cells was still strong.The filtration rate of the fixed K562 cells with formaldehyde was significantly decreased,and the average diameter of the filtered cells had no obvious change.Conclusion The living cells are able to pass the membranes with the pore sizes less than their diameters.The living cells passed the filter membranes may still maintain their growth and proliferation activity.However,the fixation of formaldehyde may significantly reduce the number of cells passed the membrane.

Objective To establish a monitoring database of historical Oncomelania hupensis environments at grass?root lev?el for schistosomiasis prevention based on Google Earth(GE),so as to improve the management efficiency of historical O. hu?pensis environments. Methods GE was labeled with the information about the historical O. hupensis environments through add?ing landmark,path and polygon. Meanwhile,POCO web album was used to storage the practice situation pictures of the environ?ments,which could be imported into GE so that the environments could be monitored dynamically. Results There were 553 historical O. hupensis snail environments in Yangjian Town,Xishan District,Wuxi City and the accumulative area was 506 000 m2. Among these environments,224(40.5%)were Class IV environments,accounting for 40.5%of accumulative area. Conclu?sions The monitoring database of historical O. hupensis environments based on GE at country level is established successfully and it can be used to manage the historical snail environments visually and monitor the changes dynamically.

Objective To determine the risk factors contributing to deep vein thrombosis (DVT) in the lower extremity after orthopedic surgery.Methods Five thousand one hundred and thirty-three patients of both sexes,aged 18-89 yr,undergoing orthopedic surgery,were included in the study.Visits to patients were performed 1 day before surgery,and age,gender,body weight,diagnosis of diseases,smoking history,complications,medical history,and results of laboratory tests were recorded.General anesthesia,epidural anesthesia or combined spinal-epidural anesthesia,or field block (brachial plexus block or femoral nerve block) was performed during surgery.Ultrasound was performed on 7th day after surgery for detection of DVT.The patients were divided into DVTfree group and DVT group.The method of anesthesia,type and duration of surgery,postoperative use of low molecular heparin for anticoagulation,time for patients resumed ambulation were recorded.Multivariate logistic regression analysis was used to examine the relationship between these factors and to pick out the risk factors for DVT.Results Two hundred and twelve patients developed DVT after surgery.The incidence of DVT was 4.13 %.Logistic regression analysis showed that age ＞ 64 yr,body mass index≥25 kg/m2,complications such as diabetes and hypertension,history of phlebeurysma,triglyceride ≥ 1.7 mmol/L,D-Dimer≥ 500 μg/L,duration of surgery ＞ 4 h,general anesthesia,time for patients resumed ambulation ≥ 5 days were independent risk factors for DVT after orthopedic surgery.Conclusion Old age,obesity,history of phlebeurysma,complications such as diabetes and hypertension,high triglyceride and D-Dimer levels,long surgery,general anesthesia,prolonged duration of bed rest after operation are independent risk factors contributing to DVT in the lower extremity after orthopedic surgery.

Objective To explore the application value of pulmonary ultrasound for the treatment of severe acute respiratory distress syndrome in children with extracorporeal membrane oxygenation( ECM O ) . Methods Seven children with severe acute respiratory distress syndrome ( ARDS ) w ho were treated with ECM O in the PICU ward of Hunan Children Hospital from August 2018 to M arch 2019 underwent pulmonary ultrasound before treated with ECM O , after each bronchoscopy and lavage , before ECM O withdrawal ,and within 24 hours after ECM O withdrawal . Performance of pulmonary ultrasound and clinical data were retrospectively reviewed . Results Seven patients treated with ECM O for 11 .5( 3－27) days ,and 1 patient underwent ECM O transport . Six ( 86 .7% ) patients were successfully weaned from ECM O ,and 1 ( 14 .2% ) patient failed to exit ECM O successfully . Four( 57 .1% ) patients were discharged from hospital , and 3( 42 .8% ) patients eventually died . In various diagnostic signs of pulmonary ultrasound were detected in 7 patients ,including pulmonary edema ( 7 cases ) ,lung consolidation ( 5 cases ) ,followed by pleural effusion ( 4 cases) ,atelectasis ( 3 cases) and pneumothorax ( 3 cases ) . Pulmonary ultrasound showed that the lungs were re‐expanded and suggested that the child could attempt to evacuate the ECM O . Conclusions Pulmonary ultrasound can comprehensively evaluate the various lung lesions in children with ECM O treatment ,and can dynamically monitor the process of lung recruitment in real time ,providing a reference for guiding the evacuation of ECM O .