The prevalence of childhood primary hypertension is increasing each year,not only bring-ing symptoms to these children,but also causing damage to multiple target organs such as heart,kidney,blood vessels,eyes and nerve system. Childhood hypertension is a risk factor of adult hypertension. Paying atten-tion to and preventing childhood hypertension has become a public health challenge. There are many associat-ed risk factors for childhood primary hypertension,including parental hypertension,overweight and obesity, low birth weight,and so forth. From the analysis of these factors,suggestions can be given about how to pre-vent childhood primary hypertension.

Cognitive function is the advanced features of brain and neonatal cognitive function has its own development characteristics. Quantitative electroencephalogram can reflect the state of brain function directly and objectively and can be used in cognitive research. Event-related potential,induced by stimulation,is the neu-roelectrophysiologic of brain cognitive processing and time-locked to cognitive events. In recent years,research-ers have made great progress in exploring the neonatal cognitive function with the help of quantitative electroen-cephalogram and event-related potential. This review summarizes the progress of these two technologies in neo-natal brain cognitive function.

Objective To investigate the aesthetic efficacy of dental implant employed to the functional reconstruction of ectodermal dysplasia (ED) patients. Methods Dental implants were inserted into the jaw bone of ED patients according clinical and X-ray examination. The dentures were made after the osseous integration of implants. The proper attachments were employed to increase the retention of the dentures. The aesthetics, denture retention, phonation, mastication, and the health of soft and hard tissues of peri-implant were evaluated in the 6~48 months follow-up period. Results 12 dental implants were inserted into jaw bone in 5 ED patients and all implants were osseointegrated and remained in the 6-48 months follow-up period. Aesthetics, denture retention, phonation, mastication, and confidence were significantly improved. Conclusions Dental implants can be used to functionally reconstruct the dentition of ED. The aesthetics and functions are improved significantly.

Objective To survye the relationshi p between blood pressure and body mass index ( BMI) ,waist-to-height ratio( WHtR) and body surface area( BSA) amogn 7 to 13 years old children in Jin-pan id strict,Haikou city.Methods A total of 1 018 school-age children aged from 7 to 13 years old were se-lected from two elementary schools in Jinpan distir ct,Haikou cti y.Blo odp ressure,height, weight and wa ist circumferencew ere measuredi n all children.BMI,WHtR,BSA were calculated and statistci al analyis s was performed by SPSS 22.0 software.Results The Pearson Correlation Coefficient of BMI and bloodp er ssure showed that BMI was posti vely correlatde with ob th systolic and id astoil c blood pressure(r=0.489,0.454, P<0.01,respective ly).The Pearos n Correlation Cofe ficient of WHtR and blood pressure showed that WHtR was positively correlated with both systolic and diastolic blood pressure(r=0.294,0.310,P<0.01,respec-tively) .The Pearson Correlation Coefficient of BSA and blood pressure showed that BSA was positively cor-related with both systolic and diastolic blood per ssure(r=0.603,0.520,P<0.01,respectively).The WHtR adn BSA of hypetr ension group were higher than those of the non-hypertension group( P<0.01) in both male and female children.Conclusion The blood pressure in 7 to 13 years old school-age children of Jinpan dis-trict,Haikou city is related to the BMI,WHtR and BSA respectively.

Coding region instability determinant (CRD) is one of the influence factors of oncogene c-myc.Coding region instability determinant-binding protein (CRD-BP) can connect with CRD in order to protect CRD from nuclease attack,prevent rapid degradation of c-myc mRNA,and increase c-myc protein content.Beta-transducin repeats-containing protein (β-TrCP) can affect cell growth,differentiation,apoptosis and oncogenesis by regulating multiple signaling pathways and cell cycle.The overexpression of CRD-BP can upregulate the expression of β-TrCP and both of them play important roles in the tumorigenesis,progression,metastasis and invasion of colorectal cancer.

Objective To investigate the feasibility of using superparamagnetic iron oxide(SPIO)as MRI contrast agent to assess rat nonalcoholic steatohepatitis Kupffer cells(KC)function.Methods Twenty male SD rats were randomly divided into A and B groups,group A(n=10)was the experimental group fed high fat diet,group B(n=10)was the control group fed normal diet After 8 weeks,plain MR and SPIO enhanced MR were performed in all the rats.Blood lipids were measured,and HE and Perl's blue staining in all livers specimen was done.The related results of the staining were analyzed with t test Results Group A TC and TG levels[(6.58±1.25)and(1.53±0.23)mmol/L respectively]were significantly higher than group B[(1.64±0.22)and(0.55±0.14)mmol/L respectively](t=11.716 and 11.588,P＜0.01).Group A and B groups hepatic signal intensity decreased in all sequences after SPIO enhanced,but in group A the level of decline[(34.78±4.51)% and(60.38±3.49)% respectively]was less than group B[(64.96±2.42)% and(81.08±1.66)% respectively]on PDWI and T_1WI,and statistically significant differences(t=-18.451 and-16.240,P＜0.01)were found.In group A the ratio of signal intensity of liver to spleen(1.002±0.141,5.000±0.516,20.004±1.490 and 2.601±0.077 respectively)was more than group B(0.400±0.102,1.500±0.115,0.503±0.105 and-0.300±0.058)before and after contrast enhancement on PDWI,T_2WI,T_2~* WI andT_1WI(t=10.745,19.800,39.168 and 92.785,P＜0.01).Typical histological hepatic lesions of NASH were observed in group A,Perl's staining-positive particles in group A(2.33±0.50)were fewer than in group B(4)(t=-10.000,P＜0.01).Conclusion The high-fat diet induced model of SD rats was close to the human NASH and was easy to establish.Clinical application of SPIO enhanced MR successfully assessed the phagocytic activity of KC in the study,and it suggested that the pathogenesis of NASH was related to the decreased phagocytic activity of KC.

Objective To construct markless gene deletion mutant at the clpP loci on the chromosome of Streptococcus mutans(S.mutans).Methods ASp resistance gene was amplified by PCR,to construct the Sp resistance cassette where the Sp resistance gene was flanked with two loxP site.After the clpP gene was cloned into the pGEM-T-Easy TA cloning vector,it was digested and linked with the Sp resistance cassette,yielding homologous recombination vector pIB △ clpP-Sp.The vector was linearized and used for the transformation of S.mutans UA159,with transformants selected on TPY plates containing Sp.The selected strain was transformed with the thermosensitive plasmid pCrePA to excise the Sp resistance gene.The pCre-PA was then easily eliminated at nonpermissive temperature,resulting in a markless mutant strain carrying a deletion at the clpP loci,which was verified by PCR and DNA sequencing.Results The result of the PCR analysis and DNA sequencing indicated that a part of the clpP gene was deleted.There was a loxP at this loci without the Sp resistance gene.Conclusion The markless clpP-deletion mutant of S.mutans was constructed successfully,which laid a foundation for further study of its biological function and its influence on the cariogenicity of S.mutans.

Objective To evaluate the interference of 13 kinds of β‐lactam antibiotics in fructosamine detection .Methods A se‐ries of solution of antimicrobial drugs were formulated with water for injection ,and added into the mixture fresh serum volume by 5% .Interfere with antimicrobial drugs on the test results of fructosamine were evaluated .Results When the blood concentrations of cefathiamidine and ticarcillin sodium/clavulanate potassium were up to 37 .5 mg/L and 247 .5 μg/mL respectively ,they began to produce positive interference on fructosamine detection .However ,the degree of interference was not correlated to the serum concen‐tration of fructosamine .Conclusion Cephathiamidine and ticarcillin sodium/clavulanate potassium have positive interference on fructosamine detection .

Objective To investigate the characteristics of cognitive development in different aged neonates.Methods Sixty-two newborns were randomly selected from relatively normal full-term babies in Changzhou Children's Hospital from December 2013 to September 2015.Electroencephalogram (EEG) and event-related potentials (ERP) were recorded with the auditory Oddball paradigm.Cognitive EEG delta power and the N2 wave area of different ages (1-10,11-20 and 21-28 days) were compared.Paired t test,analysis of variance and the LSD test were used for statistical analysis.Results (1) Delta power in the resting and cognition state:neonatal cognitive delta power in the 11-20 and 21-28 days old groups was (268.22± 132.09) and (236.01±97.40) μ V2,respectively,significantly higher than the resting delta power of the same groups [(175.80 ± 80.80) and (178.78 ± 104.74) μ V2,t=2.539 and 2.845,P=0.020 and 0.010,respectively].(2) Cognitive delta power in different aged neonates:cognitive delta power in the 11-20 and 21-28 days old groups was (268.22± 132.09) and (236.01 ±97.40) μ V2,respectively,higher than that of the 1-10 days old group [(116.70± 56.70) μV2],with statistically significant difference (LSD test,both P＜0.05).(3) Neonatal ERP:ERP of the 1-10 days old group presented with multiple peaks of a flat composite wave,in the 11-20 and 21-28 days old groups,the N2 wave showed a regular and rising trend,gradually to a single wave,and became gradually mature.The N2 wave area in the 11-20 and 21-28 days old groups was (6 435.08±2 212.34) and (6 536.75± 1 969.86) ms · μ V,respectively,which was larger than that in the 1-10 days old group [(4 230.04± 1 550.55) ms · μ V] (LSD test,both P＜0.05).Conclusions Neonatal cognitive development is enhanced with age and there may be a period of more rapid cognitive development,especially at 11-20 days of age.

Objective To investigate the effects of Zuogui Jiangtang Yishen decoction (ZGJTYS) on the expression of nephrin and podocin in podocyte of MKR mice with diabetic nephropathy (DN), and explore its mechanism. Methods Forty MKR mice (8 weeks old) were randomly divided into four groups as follows:negative control group (group A), DN model group (group B), ZGJTYS group (group C) and positive control group (group D, Gliquidone and benazepri). All mice from group B, C and D were received high-fat diet feed and unilateral nephrectomy. Four weeks after operation, all mice were received drug intervention, and four weeks later, all mice were put to death. The levels of UmAlb were observed by ELISA, the serum BUN and Cr by biochemical, and the FBG by electrochemical detection method. The nephrin and podocin mRNA expression were measured by RT-PCR and the protein expression by western blotting. The morphological structure changes of the podocytes were observed by transmission electron microscopes. Results As compared with group A, FBG, BUN, SCr and urine UmAlb in the mice of group B increased significantly (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly decreased (P<0.01). After intervention of drugs, all biochemical indicators above in the mice of group C and D significantly decreased (P<0.01), the expression level of nephrin and podocin mRNA and protein were markedly increased (P<0.01, P<0.05), compared with group B. The renal pathological lesions of group C and D were significantly improved compared with group B. Conclusion ZGJTYS decoction exerts reno-protective effect via reinstating nephrin and podocin expression to repair the damaged podocyte.