1.Vitamin D and cerebral infarction
International Journal of Cerebrovascular Diseases 2016;24(4):356-359
Vitamin D is a group of steroid hormones.Studies in recent years have shown that vitamin D is associated with the incidence of cerebral infarction,the severity of infarction,and functional outcome.In addition to the indirect effect of vascular risk factors,vitamin D may also play the direct protective effect by inhibiting inflammation and oxidative stress after cerebral infarction.
2.The Experience of Gynecological Probation Teaching of Foreign Student
Chinese Journal of Medical Education Research 2003;0(02):-
To prove the clinical teaching of gynecology of medical foreign students,this paper concludes and analyses the problems of gynecological probation teaching of foreign student through the clinical teaching work,including difficulties in language communication,no unified textbook,particularity of gynecology,and cultural differences.
3.Simultaneous Determination of Homovanillia Acid and Vanillymandelic Acid in Human Urine by HPLC
Wenjing ZHANG ; Jie LI ; Zhihu ZHANG
Journal of Environment and Health 1993;0(03):-
Objective To establish an HPLC-FLD method for simultaneous determination of homovanillic acid(HVA) and vanilmandelic acid(VMA) in human urine.Methods After being filtered with 0.45 ?m membrane,the samples of urine were injected directly into an ODS column(250 mm?4.6 mm,5.0 ?m) at the room temperature.The samples of urine were carried with the mobile phase comprised of methanol-0.1mol/L phosphate buffered solution(20:80,V/V).The flow rate was 1 ml/min,the injection volume was 10 ?l,the detection was taken at ?ex=277 nm,?em=320 nm.Results The determination was finished in 15 min,the retention time was 3.18 min for VMA and 6.72 min for HVA respectively.The detection limit of HVA was 0.15 ?g/ml,the linear range was 0-25 ?g/ml,the recovery rates were between 84.53%-106.1%,the relative standard deviation(RSD)
4.Clinical analysis of magnesium sulfate combined with nifedipine in treatment of pregnancyinduced hypertension
Jian ZHANG ; Wenjing ZHANG ; Hongxia LI
Chinese Journal of Postgraduates of Medicine 2012;35(9):29-31
ObjectiveTo investigate the clinical eflicacy of magnesium sulfate combined with nifedipine in treatment of pregnancy-induced hypertension.MethodsOne hundred and fifty pregnancyinduced hypertension patients were divided into two groups by random digits table with 75 cases each.The observation group was treated with magnesium sulfate combined with nifedipine.The control group was treated with magnesium sulfate.Then the blood pressure before and after treatment and the pregnancy outcome were observed.ResultsThe systolic blood pressure,diastolic blood pressure after treatment in observation group were lower than those in control group [ (133.2 ± 7.9) mm Hg (1 mm Hg =0.133 kPa) vs.(142.5 ± 8.0)mm Hg,(80.6 ± 6.1 ) mm Hg vs. (86.6 ± 6.1 )mm Hg],and there were significant differences between two groups (P < 0.05).The spontaneous labor rate in observation group was higher than that in control group [ 65.3% (49/75) vs.41.3% (31/75 )],and there was significant difference between two groups (P < 0.05 ).The incidence of placental abruption,hypoproteinemia,postpartum hemorrhage,fetal growth retardation,fetal distress,premature delivery,asphyxia neonatorum,perinatal mortality in observation group were lower than those in control group [ 1.3%(1/75) vs.12.0%(9/75),13.3%(10/75) vs.33.3%(25/75),8.0%(6/75)vs.16.0%(12/75),2.7%(2/75) vs.9.3%(7/75),2.7%(2/75) vs.10.7%(8/75),2.7%(2/75) vs.17.3%(13/75),1.3% (1/75) vs.5.3% (4/75),0 vs.5.3% (4/75)],and there were significant differences between two groups(P <0.05). Conclusion Magnesium sulfate combined with nifedipine in treatment of pregnancy-induced hypertension can effectively reduce the adverse pregnancy outcome,and reduce the asphyxia neonatorum rate and caesarean section rate.
5.Clinical analysis of methotrexate combined with mifepristone in the treatment of unruptured ectopic pregnancy
Jian ZHANG ; Hongxia LI ; Wenjing ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(7):981-982
ObjectiveTo observe the clinical effect of methotrexate combined with mifepristone in the treatment of unruptured ectopic pregnancy.Methods96 cases of unruptured ectopic pregnancy patients were randomly classified into two groups,observation group with 48 cases was treated with methotrexate combined with mifepristone and control group with 48 cases was only treated with methotrexate.The treatment effect and adverse reaction between two groups were compared.ResultsThe time of blood β-HCG recovery to normal and hospitalization time in observation group were significantly shorter than those in control group( P < 0.05 ),and mass diameter was significantly smaller than that in control group( P < 0.05 ).The adverse reaction incidence in observation group was 10.4% ( 5/48 )while 6.3% (3/48) in control group.ConclusionMethotrexate combined with mifepristone in the treatment of unruptured ectopic pregnancy could enhance clinical effect,shorten treatment time,decrease adverse reaction,which could be applied in clinic.
6.Analysis of clinical characteristics and prognostic factors in 92 cases of endometrial cancers
Wenjing ZHANG ; Jian ZHANG ; Qingbing WANG
Chinese Journal of Postgraduates of Medicine 2011;34(12):11-12
Objective To study the dependablity of clinical characteristics and prognosis in patients with endometrial cancer,so as to apply the basis for the treatment. Methods The clinical and pathological data of 92 patients with endometrial cancer were analyzed retrospectively, 5-year survival rate was followed and observed. Using Cox proportional hazard regression analysis to evaluate the factors related to overall survival. Results The overall 5-year survival rate was 72.41%. Using univariate analysis, the survival time was correlated with histologic type, pathological staging, histologic grade, myometrium invasion,cervical invasion and lymph node metastasis (P<0.05).Using multivariate analysis, the survival time was correlated with histologic type,histologic grade and myometrium invasion. Conclusions Histologic type, histologic grade and myometrium invasion are independent prognostic factors for survival time of patients. In order to improve the prognosis of the patients, they should be treated by individual therapies.
7.Role of angiotensin Ⅱ receptors in development of neuropathic pain in rats
Wenjing ZHANG ; Xiaolin ZHANG ; Yanqing CHEN
Chinese Journal of Anesthesiology 2014;34(6):715-717
Objective To evaluate the role of angiotensin Ⅱ receptors in the development of neuropathic pain (NP) in rats.Methods Seventy-five male Sprague-Dawley rats,weighing 230-270 g,were randomly divided into 5 groups (n =15 each) using a random number table:sham operation group (group S),group NP,telmisartan (angiotensin Ⅱ receptor antagonist) 2 mg group (group T1),telmisartan 5 mg group (group T2),and telmisartan 10 mg group (group T3).NP was induced by chronic constrictive injury (CCI) in chloral hydrateanesthetized rats.The sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread.In S group,the sciatic nerves were exposed but not ligated.Telmisartan 2,5 and 10 mg/kg were perfused via stomach once a day for 14 consecutive days in T1,T2 and T3 groups,respectively,while the equal volume of normal saline was given instead of telmisartan in S and NP groups.Mechanical and thermal pain thresholds were measured at 1 day before CCI and 3,7 and 14 days after CCI in each group.Results The mechanical and thermal pain thresholds were significantly decreased after CCI in NP,T1,T2 and T3 groups as compared with group S.The mechanical and thermal pain thresholds were significantly increased after CCI in T1,T2 and T3 groups as compared with group NP.The mechanical and thermal pain thresholds were significantly higher after CCI in T2 and T3 groups than in group T1.There was no significant difference in the mechanical and thermal pain thresholds between T2 and T3 groups.Conclusion Angiotensin Ⅱ receptors are involved in the development of NP in rats.
8.Experimental Study on Relation between Analgesic Action of Alcohol Extraction of Natrix tigrina Lateralis and Central Nervous System
Wenjing ZHAO ; Qiao ZHANG ; Shulian LI
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(05):-
Objective To Study the relation between analgesic action of alcohol extraction of Natrix tigrina Lateralis and central nervous system. Methods Manifold ache models of experimental nerve centre were established to observe the analgesic action and degree of alcohol extraction of Natrix tigrina Lateralis to the ache models. Results Alcohol extraction of Natrix tigrina Lateralis had analgesic action to all ache models of nerve centre, and the high-dose group was obviously. Conclusions Analgesic action of alcohol extraction of Natrix tigrina Lateralis is related to effecting on central nerves system, which effects antinociceptive in central nervous.
9.Investigations on Air Quality in the Basement of High Sciece-Technologic Buildings
Jin ZHANG ; Yaoming CHEN ; Wenjing LUO
Journal of Environment and Health 1992;0(02):-
Objective To investigate air quality in the basements of high science-technologic buildings. Methods Continuous tests of air quality parameters of physics, chemistry and microbiology were conducted in 11 rooms of the basement used for working and living. Results At every test point the average room temperature was 25.5 ℃, the average relative humidity was 78.7 per cent, the average wind velocity was 0.057 m/s. Between the relative humidity and temperature(r=-0.850, P
10.Establishment of topotecan-resistant SKOV3/TPT cell line of human oophoroma and a study of its biological features
Youzhen ZHOU ; Hongxia LI ; Wenjing ZHANG
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To establish the topotecan(TPT)-resistant cell line of human oophoroma (SKOV3/TPT), and to investigate its biological features. Methods To simulate the condition in clinical chemotherapy, drug resistant SKOV3/TPT cell line of human oophoroma was established by challenging SKOV3 with a large dose of TPT (2160ng/ml). Drug sensitivity was assessed by MTT assay. Light microscopy, electronic microscopy, trypan blue staining rejection, and cell counting were employed to determine the biological features of the cell line. Flow cytometry (FCM) was used to study the cell cycle distribution, the accumulation of intracellular drug, and the expression of P glucoprotein (Pgp). Results SKOV3/TPT cells were established successfully, and the resistance index (RI) was 10.67. They showed an obvious cross-resistance to CPT-11, MX and VCR, but still sensitive to taxol, cis-platin, VP16, MTX and 5-Fluorouracil. Comparing with the parent cells, their morphology was unchanged, but multiplication time was prolonged. The apoptosis rate and the proportion of G2+M phase cells of resistant cells were significantly higher than that of their parent cells (P