1.Significance and change of SCF/KIT following cerebral ischemia reperfusion
Yun LUO ; Wenjing ZHU ; Yun XU
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To study the expressions and significance of stem cell factor(SCF) and tyrosine kinase receptor(KIT) in neurons after cerebral ischemia reperfusion injury.METHODS: Western blotting was used to examine the expression of SCF and KIT in the cortex after cerebral ischemia and oxygen glucose deprivation(OGD) neurons,then the OGD neurons preincubated with SCF and SCF-Ab were assessed the cell viability by MTT to determine its significance.RESULTS: ①Compared to control group,the expressions of M-SCF and S-SCF in cortex increased markedly while KIT decreased significantly in ipsilateral hemisphere.However,no obvious change in the contraleteral hemisphere was observed.②Compared to control group,the levels of S-SCF and M-SCF were increased significantly in the OGD neurons while KIT was not obvious changed.③The cell viability of OGD neurons decreased significantly.Preincubation with SCF inhibited cell damage while SCF-Ab deteriorated OGD injury.CONCLUSION: The expression of SCF may be induced by cerebral ischemia reperfusion,which can protect the neurons from ischemia injury.
2.Determination of Methyl Tertiary Butyl Ether in Cerebrospinal Fluid of Rats by Gas Chromatography
Yun ZHANG ; Wenjing LUO ; Ting YAO
Journal of Environment and Health 2007;0(12):-
Objective To establish a method for the determination of methyl tertiary butyl ether (MTBE) in the cerebrospinal fluid of rats by gas chromatography and to know whether MTBE can penetrate the blood brain barrier. Methods 9 male SD rats were randomly divided into 3 groups and then exposed to MTBE by gavage at a dose of 1000 mg/kg?bw and by inhalation at a dose of 8 ml/L for 30 days. The cerebrospinal fluid was collected at 6 h, 12 h and 24 h respectively at the end of treatment and the MTBE in rats cerebrospinal fluid was determined by gas chromatography. Results MTBE was detected in the cerebrospinal fluid of rats after subchronic exposure of MTBE through gavage and inhalation. Conclusion MTBE is able to penetrate the blood brain barrier and reach the brain.
3.SCF protects the cortical neuron from apoptotic cell death in diabetic mice
Yun LUO ; Wenjing ZHU ; Fengnan NIU ; Yun XU
Chinese Journal of Nervous and Mental Diseases 2009;35(12):730-733
Objective To investigate the role of SCF on neuronal apoptosis induced by diabetes and its possible mechanism.Methods Twenty-seven male C57 mice were randomly divided into control group,diabetes group,and diabe-tes plus stem cell factor(SCF)group.The diabetic mice were induced by streptozotocin.TUNEL staining was used to assess neuronal apoptosis and western blot were used to detect the protein level of BCL-2,BAX,CASPASE 3 and P-ERK/ERK.Results Compared with the controls,the number of apoptotic neuron death and the protein levels of active CASPASE 3 were significantly increased in the cortex of diabetic mice.Treatment with SCF significantly reduced apoptotic neuron death and attenuated the increased in protein levels of active CASPASE 3 in the cortex of diabetic mice.The levels of BCL-2 and BAX were significantly increased in the diabetic animals compared to the controls.Treatment with SCF could significantly attenuated the increase in the expression of BAX but could not affect the level of BCL-2 in the cortex of diabetic mice.P-ERK was significantly decreased in the diabete group but not in dibete plus SCF group.Conclusions SCF can protect a-gainst diabete-induced apoptotic neuron death through increasing the phosphorylation of ERK and influencing the expression of BCL-2/BAX.
4.Effect of Chronic Episodic Hypoxia on Liver CYP3A_2 and CYP2E_1 in Rats
Wenjing ZOU ; Binghua WANG ; Yun WANG ; Lida CHEN ; Ying ZHENG
China Pharmacy 2001;0(12):-
OBJECTIVE: To investigate the effects of episodic hypoxia(EHYP) on liver CYP3A2 and CYP2E1. METHODS: Healthy masculinity rats were divided randomly into five groups: control group, 3 -day EHYP group, 7 -day EHYP group, 14 -day EHYP group, and 28 -day EHYP group. 24 h after the management of EHYP, the rats were anesthetized by intraperitoneal injection, and the eyeball blood 2ml was drawn to prepare serum. The activities of ALT and AST in the serum were determined by enzymatic methods. The activities of liver microsomal erythromycin demethylase(ERD) and aniline hydroxylase(ANH) were detected by spectrophotography. The levels of mRNA expression of CYP3A2,CYP2E1 were assayed with RT-PCR. RESULTS: The serum activities of ALT,AST showed no remarkable change. However, after 7 days of hypoxia, ERD and ANH activities were remarkably elevated, and at 28 days induction rates reached up to155.5% and 42.2%, respectively. Meanwhile, the levels of mRNA expression of CYP3A2,CYP2E1 also increased by 220.5% and 102.8%, respectively. CONCLUSION: Chronic EHYP can remarkably increase the activities of ERD and ANH, whose mechanism is probably related to up-regulating of CYP3A2 and CYP2E1 expression at the transcriptive level.
5."Based on ""pulmonary gangrene"" warming yang and supporting, promoting blood circulation and Huoxuegongtan method for treatment of pulmonary alveolar proteinosis"
Xu JIAO ; Mo ZHANG ; Wanyun HE ; Wenjing LAI ; Yun LU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2017;24(3):320-322
Pulmonary alveolar proteinosis (PAP) is a rare disease clinically, and at present no western medicine can effectively cure the disease. After the analysis of literatures related to Traditional Chinese Medicine (TCM) for treatment of the disease in the China National Knowledge Internet Database, it was discovered that the syndrome differentiation showed the disease closely associated with phlegm, blood stasis and yin deficiency. In this study, from view point of pulmonary gangrene TCM theory and treatment combined with PAP pathophysiological characteristics, it is emphasized that phlegm accumulation, blood stasis and yang deficiency are the key pathological manifestations of PAP, thus Yanghe decoction, Tounong powder, Kongxian pellet were used for treatment of one such case, the rational thinking in diagnosis and treatment of this patient being consistent with the idea learning western medicine for China use.
6.Multiresidue determination of fluoroquinolones in eggs by solid-phase extraction-LC-MS/MS
Yuan TIAN ; Zunjian ZHANG ; Jing LI ; Wenjing LI ; Yun CHEN
Journal of China Pharmaceutical University 2010;41(1):60-65
Aim: To establish an analytical method for the simultaneous determination of norfloxacin,ofloxacin,pefloxacin,ciprofloxacin,lomefloxacin,danofloxacin,enrofloxacin,sarafloxacin and difloxacin in eggs using solid-phase extraction-LC-MS/MS.Methods: Egg samples were deproteinized with acetonitrile,followed by defatting with hexane.Then the samples were processed by solid-phase extraction and analyzed by LC-MS/MS using an electrospray source.The separation was carried out on a Shimadzu Shim-pack VP-ODS C_(18) column,with a mobile phase consisting of acetonitrile-0.1% formic acid(13: 87).Results: The validated method was proved to be of high specificity,accuracy and sensitivity.Conclusion: The established method is suitable for the routine residual monitoring of fluoroquinolones.
7.Correlation study of multimodal ultrasound characteristics with HCK and MRPL13 expression in breast cancer
Yunfang DU ; Yuwang ZHOU ; Yun FANG ; Wenjing TONG ; Hongmei ZHOU
Chinese Journal of Endocrine Surgery 2021;15(2):134-140
Objective:To investigate the correlation between hematopoietic cell kinase (HCK) and the expression level of the mitochondrial ribosomal protein L13 (MRPL13) and hematopoietic multimode ultrasound.Methods:204 female breast cancer patients treated by surgery in Quzhou people’s Hospital from Jan. 2017 to Sep. 2020 were selected as study subjects. Breast cancer tissues and adjacent normal tissues were extracted intraoperatively. Preoperative conventional ultrasound, shear wave elastography (SWE) and contrast-enhanced Ultrasonography (CEUS) were used to detect HCK and MRPL13 expression levels. Univariate analysis and binary Logistic regression were used to analyze the correlation between multi-mode ultrasonic features and HCK and MRPL13.Results:The positive expression ratios of HCK and MRPL 13 in breast cancer tissues were significantly higher than those in adjacent tissues ( χ2 was 5.625, 7.197; P was 0.018, 0.007) . In conventional ultrasound features, the proportions of HCK-positive breast cancer patients with irregular mass edges, microcalcifications, and grade II to III blood flow classification were significantly higher than those of HCK-negative patients ( χ2 was 7.437, 16.684, 23.262; P was 0.006, <0.001, <0.001) ; The proportion of MRPL13-positive breast cancer patients with a maximum diameter of ≥2 cm, irregular edges of the tumor, and grade II-III blood flow classification was significantly higher than that of MRPL13-negative patients ( χ2 was 4.676, 11.118, 8.389; P was 0.031, 0.001, 0.004) . For SWE signs, the proportion of HCK positive breast cancer patients with hard ring sign was significantly higher than that of HCK negative patients ( χ2=11.220, P=0.001) ; the proportion of MRPL13 positive breast cancer patients with hard ring sign and black hole sign was significantly higher than that of MRPL13. Those who were negative ( χ2 was 4.482, 8.775; P was 0.034, 0.003) . Among CEUS characteristics, the proportion of HCK-positive patients with high enhancement was significantly higher than that of HCK-negative patients ( χ2=7.356, P=0.007) ; the proportion of MRPL13-positive patients with high enhancement and late regression was significantly higher than that of MRPL13-negative patients ( χ2 was 9.165, 7.631; P was 0.002, 0.006) . The results of binary logistic analysis showed that there was microcalcification ( OR=4.619, 95% CI=2.657-8.119, P=0.009) , blood flow classification II to III ( OR=4.150, 95% CI=2.547-7.954, P=0.015) and high enhancement of CEUS ( OR=4.150, 95% CI=2.547-7.954, P=0.015) are independent risk factors for positive expression of HCK; blood flow grade II to grade III ( OR=4.213, 95% CI=3.145-8.557, P=0.012) , appearance of black hole sign ( OR=5.246, 95% CI=2.864-10.378, P<0.001) and high enhancement of CEUS ( OR=3.872, 95% CI=1.887~6.438, P=0.026) were the independent risk factors for the positive expression of MRPL13. Conclusion:The multimodal ultrasonographic features of breast cancer are helpful to predict the expression levels of HCK and MRPL13, so as to provide new imaging ideas for early diagnosis of breast cancer, the designation of treatment options and the preoperative non-invasive assessment of breast cancer prognosis.
8.Induction of apoptosis in PC12 cells by homocysteine
Jinxiu CAO ; Binghua WANG ; Yun WANG ; Lida CHEN ; Wenjing ZOU ; Ying ZHENG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To explore the effects of homocysteine on the apoptosis in PC12 cells and the relationship between the apoptosis and the expression of the bcl-2 as well as bax gene. METHODS: Cell viability was determined by MTT assay. Cell apoptosis was assessed by phase-contrast microscope, fluorescence microscopy and flow cytometry (FCM). The expression of bcl-2 and bax mRNA was measured by semiquantitative reverse transcription polymerse chain reaction (RT-PCR). RESULTS: Treatment of PC12 cells with Hcy plus 10 ?mol/L copper for 12 h, in the range of 0.125, 0.25, 0.5, 1.0 mmol/L, caused a great decrease in cell viability: 81%, 79%, 69%, 57%, and induced typical morphological changes of apoptosis in a dose-dependent manner. The apoptosis ratios were respectively 8.00%, 10.37%, 17.26% and 20.19%, respectively. The expression of bcl-2 was significantly decreased (from 0.517 to 0.198) whereas bax was significantly increased (from 0.302 to 0.619). CONCLUSION: Homocysteine plus copper may induce apoptosis in PC12 cells through the down-regulation of bcl-2 and the up-regulation of bax gene expression.
9.Research on Application of Evidence-based Medicine Education Pattern in the General Practitioners' Standardized Training
Hongmei LI ; Hujiaaihemaiti MINAWAER ; Jie QIN ; Lijuan MA ; Wenjing JI ; Yun WU
Chinese Journal of Medical Science Research Management 2015;28(6):475-477
Objective To compare the application of evidence-based medical education and traditional medical education in the general practitioners' standardized training.Methods 80 students accepting general practitioners' standardized training were researched to compare the teaching effect of evidence-based and traditional medical education.Results Application of evidence-based medical education help to improve learning interests and learning abilities subjectively,and it helps improve exam results objectively.Conclusions Application of evidence-based medical education in the general practitioners' standardized training receives good learning effect and can help achieve the learning goal.
10.Characteristics of spatial distribution of water fluoride in Heze City, Shandong Province based on inverse distance weighted
Wenjing ZHANG ; Jianchao BIAN ; Zhongjie YUN ; Peizhong CHENG ; Guodong SUN ; Hongxu GAO ; Jie GAO
Chinese Journal of Endemiology 2014;33(2):178-181
Objective To evaluate the inverse distance weighted(IDW) in revealing the characteristics of spatial distribution of water fluoride in Heze City,Shandong Province.Methods A geographic information system (GIS) database of water fluoride was established in Heze City of Shandong Province using the data of endemic fluorosis surveys collected by Endemic Disease Prevention Institute in Shandong Province during 2005-2007.IDW spatial interpolation was applied to predict the distribution of fluoride in drinking water in 139 towns of Heze City.Sensitivity and specificity were calculated.Results Mean water fluoride levels in 10 counties of Heze City were all higher than 1.0 mg,/L,and the water fluoride in Cao County,Juye,Mudan District and Juancheng were higher than 2.0 mg/L.Of all 139 townships of Heze City,129 were higher fluoride townships where fluoride was > 1.0 mg/L,10 were lower fluoride townships(≤ 1.0 mg/L).IDW spatial interpolation showed that the water fluoride of most areas in Heze City were > 1.0-2.0 mg/L.The areas with water fluoride of > 2.0-3.0 mg/L were mainly located in eastern Juancheng,northern Mudan District,north-central Chengwu,central and southern Juye,southeastern part of Caoxian and eastern part of Shan Town.Regions of water fluoride > 3.0 mg/L were mainly distributed in Xieji and Wanfeng towns of Juye County,Jishan town of Juancheng County,Sunlaojia town of Caoxian and Dusi town of Mudan County.The internal verification results showed that the sensitivity,specificity and overall accuracy rate of IDW used for predicting water fluoride content was 100.00% (129/129),10.00% (1/10) and 93.53% (130/139),respectively.While the external verification results showed that the sensitivity,specificity and overall accuracy rate of IDW for predicting water fluoride content was 100.00%(31/31),11.11%(1/9) and 80.00%(32/40),respectively.Conclusion With the application of IDW interpolation,it is feasible to infer the overall spatial distribution based on the monitoring data,and to reveal the spatial characteristics of water fluoride in Heze City,Shandong Province.