[Objective]To understand the alienation of contemporary col ege students casual situation and problems, discuss col ege student leisure time value of values education and role. [Method] Horizontal analysis between the U.S. and Chinese universities, col eges and universities within the longitudinal anal-ysis of the current situation of leisure education.[Results] Alienation of contemporary col ege students casual question has three aspects: the lack of leisure and skil s to form a single, low-level leisure and disorganized ,leisure concepts matter of this theory. [Conclusion] Propose to strengthen values education col ege of casual modern higher education development is a necessary requirement for students to achieve an effective way to cultivate harmonious person-ality , is the solution of recreational alienation practical needs of higher education which should not be overlooked as an important part.

OBJECTIVE To find out the knowledge and understanding level of prevention from needle-stab for practice nurses to train and educate their professional safety precautions. METHODS There were 105 practice nurses who practiced in the hospital from Jul 2003 to Apr 2004,and were asked for filling out the inquiry paper forms which made by ourselves. RESULTS We found that only 20 nurses were good at understanding their professional safety precautions which occupied 19.05%.Fifty nine nurses understood just so-so which occupied 56.19%.Twenty six nurses were poor-understood which occupied 24.76%. CONCLUSIONS The results are not satisfied with the practice nurses,so we suggested the medical school teachers and the hospital supervisors think highly of training and educating the practice nurses. Let them learn and understand the knowledge of the professional safety precautions.Restricted the training and education plan and countermeasures to avoid the blood infectious diseases.

Objective To evaluate the effect of different early nutrition support on nutritional status,immune function and stress condition in patients with severe craniocerebral trauma.Methods 90 postoperative patients with severe craniocerebral trauma were randomly divided into standard EN group,immune EN group and TPN group,30 cases in each group.Blood samples were obtained for measurement nutritional status,immune function and stress conditionin in patients before treatment and the first,seventh and twenty first day after surgery.Results Nutritional status,immune function and stress condition of patients in immune EN group improved significantly after seven days.Nutritional status,humoral immune function and stress condition of patients in standard EN group also improved.After 21days,nutritional status,humoral immune function and stress condition of patients in TPN group improved (P ＜ 0.05 or P ＜ 0.01).Conclusion Early immune EN nutrition support of patients with severe craniocerebral trauma can improve nutritional status,immune function and stress condition and maybe the best nutrition support method.

Objective To investigate the relationship among ultrafiltration (UF), diurnal rhythm of blood pressure (BP) and cardiac structure and function in hemodialyzed(HD) patients. Method 42 hemodialyzed patients were studied. Intensified UF during routine HD were given to them for 4 weeks. Before and after 4 weeks, everyone received 24 hours ambulatory blood pressure monitoring and cardiac doppler and recorded diameter of left atrium (LAD) ,left ventricle (LVEDD), thickness of posterior wall of left ventricle during end stage of diastolic period (LVPWT) ,thickness of interventricular septum(IVST) ,ejection fraction (EF). Result In hemodialyzed patients, non-dipper hypertension was much more than dippers (P < 0.05). There was significant difference between nocturnal BP and cardiac structure before and after 4 weeks of hemodialysis ( P < 0.05 ). Conclusion Nocturnal hyperten- sion of hemodialyzed patients is related to hypervolemia. Intensive ultrafihration can improve nocturnal hypertension and eardiac function.

Objective To analyze and evaluate the relationship between hepoatocelluar carcinoma incidence and hepatitis B e antigen (HBeAg) expression in choronic hepatitis B (CHB) patients with long-term treatment of nucleoside analogue therapy.Methods The clinical data of 56 CHB patients with long-term treatment of nucleoside analogue therapy except for hepoatocelluar carcinoma (experimental group)were summarized retrospectively,and all patients underwent anti-viral therapy with no less than 5 years.The clinical data of 56 CHB patients without anti-viral therapy in the same period (control group) were collected.The risk factors of hepoatocelluar carcinoma were analyzed.Results The incidence of hepoatocelluar caroinoma was 7.14％ (4/56) in experimental group and 19.64％ (11/56) in control group,and there was significant difference (P ＜ 0.05).The incidence of hepoatocelluar carcinoma was 0 in patients with positive HBeAg without cirrhosis (36 cases) in experimental group and 1/8 in patients with negative HBeAg without cirrhosis in experimental group,and there was significant difference (P＜ 0.05).Conclusions Long-term treatment of nucleoside analogue therapy can reduce but bot eliminate the hepatocellular carcinoma risk in patients with CHB,especially in those with negative HBeAg.HBeAg negativity is a significant risk factor for the development of hepallerular carcinoma in CHB patients without liver cirrhosis.

Objective To investigate the concentration of secreted protein acidic and rich in cysteine (SPARC) in body fluid of patients with autosomal dominant polycystic kidney disease (ADPKD) and the origin of its secretion. Methods The concentration of SPARC in plasma, urine and cystic fluid from patients with ADPKD and healthy individuals was measured with ELISA. The content of SPARC protein in the culture medium of cystic epithelial cells was detected by Western blot. Results The concentration of SPARC in cyst fluid of ADPKD patients [( 3 628.75? 1 445.90) ng/ml] was much higher than that in their plasma and urine (P

Aim To observe the effects of amlodipine on cell cycle,cell cycle-related genes and cyclin expression of human breast carcinoma MCF-7 cells.And to probe effects of amlodipine on cell cycle and its mechanism of human breast carcinoma MCF-7 cells.Methods In vitro growth inhibitory effects of amlodip-ine on human breast carcinoma MCF-7 cells were determined by MTT assay,cell cycle distribution was detected by flow cytometry,the Mrna expression levels of cell cycle-related genes cyclinD1 and p21 were treated by RT-PCR,and the protein expression of cell cycle protein cyclinD1 and p21 were assessed by Western blot.Results With dose and time dependently,amlodipine could inhibit the proliferation of human breast carcinoma MCF-7 cells in vitro.The IC_(50) was 14.439 μmol·L~(-1).When breast cancer cells MCF-7 were treated with 7.22 μmol·L~(-1)(1/2 IC_(50)),14.439 μmol·L~(-1)(IC_(50))and 28.88 μmol·L~(-1)(2IC_(50))amlodipine for 48 h,the ratios in the G_0/G_1 phase were significantly increased as compared with control group(P<0.05).Amlodipine inhibited the expression of Mrna and protein of cyclinD1,while increased the expression of Mrna and protein of p21.Conclusions Amlodipine exhibits obvious anti-tumor activities on human breast carcinoma MCF-7 cells and arrest cell cycle in G_1 phase.The mechanism of G_1 phase arresting may be related to modulating the Mrna and protein expression of cell cycle-related gene cyclinD1 and p21.

[Objective]To evaluate the effect of post-operative rehabilitative nursing of patients repaired with acellular nerve allograft.[Method]From April 2003 to April 2006,39 inpatients with peripheral nerve defect were subjected to receive acellular nerve allograft in order to repair nerve defect.The patients were rehabilitated with special nursing after being operated and discharged.Among of them,21 patients were followed up over 6 months,the effect of treatment was analyzed.[Result]Among 21 patients,16 people had excellent and good effect of treatment and the efficient rate was 71.4%.[Conclusion]Post-operative rehabili tative nursing is important and effective for rehabilitation patients of peripheral nerve injuries repaired with acellular nerve allograft.

AIM: To establish a novel method for the overall quality control of Fructus Gardeniae by HPLC digitized fingerprint and its normalization. METHODS: The chromatographic fingerprints were obtained by injecting 5 ?L of the sample solution each time on a Century SIL C_ 18 BDS column (20 cm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acid- acetonitrile. The flow rate was 1.0 mL /min, the column temperature was maintained at (30?0.15)℃ and the detection wavelength was set at 265 nm. The chromatographic fingerprints were evaluated by the digitized parameters, such as the chromatographic fingerprint index (F), quantitative similarity, etc,. The influence of big peak on similarity was elaborated by direction cosines, and its cancellation was achieved by the qualitative similarity of peak area ratio. RESULTS: 35 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae by taking Geniposide peak as the reference peak. The HPLC digitized fingerprint and its normalization were implemented. CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Fructus Gardeniae.

AIM:Capillary electrophoresis fingerprints(CEFP) and quantitative analysis methods of Radix Scutellariae were established. METHODS:All experiments were carried out in an uncoated fused silica capillary(75 cm?75 ?m i.d.,effective length 63 cm) with a 50 mmol/L sodium borate solution(contained 5% acetonitrile,pH 9.30) under 12 kV while the detection wavelength was set at 280 nm and naproxen was selected as the internal standard. RESULTS:8 co-possessing peaks were selected in the CEFP taking naproxen referential peak.The similarities between each of the ten places and the referential CEFP of Radix Scutellariae were evaluated both the qualitative similarity S_F and the quantitative similariy Q,the apparent quantitative similarity R. CONCLUSION:The method of the CEFP and quantitative analysis are rapid,simple and accurate with a good repeatability and can be used for the quality control of Radix Scutellariae.