OBJECTIVE: To assess the influence of FLT3 expression on the prognosis of patients with acute myeloid leukemia (AML) by cell experiment and clinical data analysis. METHODS: Models for FLT3 over-expression and interference-expression in AML cells were constructed. The level of BAK gene expression and its protein product was determined, along with the proliferation and apoptosis of leukemia cells. FLT3 gene expression and FLT3-ITD variant were determined among patients with newly diagnosed AML. RESULTS: Compared with the interference-expression group, the level of BAK gene expression and its protein in FLT3 over-expression AML cells was significantly lower (P < 0.001), which also showed significantly faster proliferation (P < 0.001) and lower rate of apoptosis (P < 0.001). The expression level of FLT3 gene among patients with newly diagnosed AML was also significantly higher compared with the healthy controls (P < 0.001). The FLT3 gene expression of FLT3-ITD positive AML patients was higher than that of FLT3-WT patients (P = 0.002). Survival analysis showed that AML patients with high FLT3 expression in the medium-risk group had a lower complete remission rate and overall survival rate compared with those with a low FLT3 expression (P < 0.001). CONCLUSION Over-expression of FLT3 may influence the course of AML by promoting the proliferation of leukemia cells and inhibiting their apoptosis, which in turn may affect the prognosis of patients and serve as a negative prognostic factor for AML.
Humans ; Apoptosis/genetics* ; Data Analysis ; Leukemia, Myeloid, Acute/genetics* ; Gene Expression ; fms-Like Tyrosine Kinase 3/genetics*

Objective:To explore the effect of emotional ABC theory-based cognitive education on the emotions of lung cancer patients and their families and quality of life of patients.Methods:From January 2019 to January 2022, 111 lung cancer patients and 111 main caregivers admitted to the First Affiliated Hospital of Zhengzhou University were selected as the study subject by convenience sampling. 55 patients and 55 main caregivers enrolled from January 2019 to June 2020 were assigned to the control group, and 56 patients and 56 main caregivers enrolled from July 2020 to January 2022 were assigned to the experimental group. The main caregivers in the control group adopted conventional cognitive education, while the main caregivers in the experimental group adopted cognitive education based on emotional ABC theory on the basis of the control group. A survey was conducted on patients and their main caregivers using the Positive and Negative Affect Scale (PANAS) and the Functional Assessment of Cancer Therapy-Lung (FACT-L) to compare the negative emotions of the two groups of main caregivers and patients, as well as quality of life of patients before and after intervention.Results:There was no statistically significant difference in PANAS scores between the two groups of main caregivers and patients before intervention ( P>0.05). After intervention, the PANAS scores of the main caregivers and patients in both groups decreased, and the scores in the experimental group were lower than those in the control group, with statistical differences ( P<0.05). There was no statistically significant difference in scores of physical well being, social/family well being, relationship with doctor, emotional well being, functional well being, additional attention, and total score in FACT-L between the two groups of patients before intervention ( P>0.05). After intervention, the scores of physical well being, social/family well being, relationship with doctor, emotional well being, functional well being, additional attention, and total score in FACT-L of the experimental group patients were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Emotional ABC theory-based cognitive education for lung cancer patients and their main caregivers can alleviate the negative emotions of the main caregivers and patients, and improve patients' quality of life.