BACKGROUND:During clinical orthodontic correction, the self-ligating bracket is more helpful to maintain the periodontal healthy than the traditional bracket, but previous studies mainly focused on the clinical periodontal index and periodontal pathogens, and whether inflammatory cytokines are involved has not been reported. OBJECTIVE:To analyze the changes of the expression levels of interleukin 1β and tumor necrosis factor α in gingival crevicular fluid before and after the correction using self-ligating bracket and conventional bracket appliance. METHODS:Totaly 38 orthodontic patients without periodontal disease (including 20 males and 18 females; aged 11-25 years) were included in this study. Al the patients were randomly divided into control and test groups (n=19/group) and subjected to orthodontic correction using self-ligating bracket and conventional bracket appliance, respectively. The volumes of gingival crevicular fluid and expression levels of interleukin-1β and tumor necrosis factor-α were detected before correction and at 4, 8 and 12 weeks after correction. RESULTS AND CONCLUSION: The volume of gingival crevicular fluid and expression levels of interleukin-1β and tumor necrosis factor-α after correction were al higher than those before correction (P< 0.05). The volume of gingival crevicular fluid and expression levels of interleukin-1β and tumor necrosis factor-α after correction in the test group were lower than those in the control group (P< 0.05). These results demonstrate that self-ligating bracket appliance is more conducive to protect the periodontal tissue than the conventional bracket appliance.

Objective:To investigate the effect of chronic ethanol consumption on sensory information transmission in the cerebellar molecular layer and reveal the mechanism of chronic alcoholism on sensory information transmission and integration in the cerebellar cortex.Methods:Fifty healthy male ICR mice aged 6-8 weeks were randomly divided into saline group(control group)and ethanol consumption group(alcohol group) according to the random number table, with 25 mice in each group.The mice in alcohol group were injected intraperitoneally with 20% ethanol daily, while the mice in control group were injected with the same dose of normal saline. All mice were injected intraperitoneally once a day for 28 days.Through electrophysiological technology, patch-clamp amplifier and data acquisition software were used to record the changes in cerebellar molecular layer field potential of mice in the alcohol group and control group induced by sensory stimulation.Clampfit 10.3 software was used to record and analyze the electrophysiological data. SPSS 22.0 software was used for statistical analysis. Paired t-test and one-way ANOVA were used to analyze the differences before and after treatment. Results:After giving the stimulation of wind blowing, the amplitude of P1 in alcohol group was significantly higher than that in control group ((121.31±3.5)%, (97.2±2.7)%; t=26.08, P<0.05), and the area under the P1 curve (AUC) of the alcohol group was significantly lower than that of the control group ((127.1±4.2)%, (102.2±3.5)%; t=22.95, P<0.05). There was no significant difference in N1 amplitude between the two groups (P>0.05). When L-NNA, an inhibitor of nitric oxide synthase, was perfused into the brain surface of mice, the amplitude of P1 in alcohol group was significantly lower than that before administration ((76.2±4.8)%, (103.5±3.6)%; t=22.60, P<0.05), but there was no difference of the amplitude of P1 before administration and after elution ((101.5±4.6)%) ( t=1.70, P>0.05). After the L-NNA was perfused, the AUC of P1 was significantly lower than that before administration((72.4±5.6)%, (102.7±2.66)% ( t=24. 58, P<0.05), and there was no significant difference between before administration and after elution( (100.6±3.5)%, t=1.81, P>0.05). When L-NNA was perfused into the brain surface of mice, the amplitude of P1 in control group was (104.3±1.6)% and it had no differences compared with before administration(102.2±5.6)%, t=1.84, P>0.05) and after elution(102.5±4.5)%, t=1.92, P>0.05). And the AUC of P1 in control group after perfused L-NNA had no differences compared with before administration(103.5±2.6)%, (102.5±4.6)%) and after elution((101.9±3.7)%, t=0.99, 1.81, both P>0.05). When the mouse brain surface was perfused with NO donor SNAP, the amplitude of P1 in the control group was significantly higher than that before administration( (128.2±3.4)%, (103.5±2.6)%; t=28.89, P<0. 05) and there was no difference between before administration and after elution( (105.4±4.2)% , t=1.93, P>0.05). The AUC of P1((125.4±4.4)%) was higher than before administration((104.3±4.6)% , t=16.60, P<0.05) and there was no difference between before administration and after elution(103.5±4.2)%, t=0.65, P>0.05). Conclusion:Chronic ethanol consumption significantly enhances the inhibitory response, and the enhancement of inhibitory components stems from the activation of the NO signaling pathway.

Objective To evaluate the value of the magnetic resonance imaging (MRI)in the diagnosis and classification of plas-ma-cell mastitis (PCM).Methods The breast MRIs of eighteen pathologically confirmed PCM patients were retrospectively ob-served and analyzed.The manifestations of lesions,including shape,size,margin,and characteristic enhancement on T1 WI,T2 WI with fat suppression and contrast enhanced T1 WI were observed and documented respectively.Results PCM were divided into three types according to MRIs:the inflammation type 4 cases,the abscess type 9 cases,and the mixed type 5 cases.In the inflammation type,3 cases of lesions were wide extending while the other 1 limited in nipple and areola.All 4 cases of lesions were hypointensity on T1 WI and hyperintensity on T2 WI with fat suppression.And in the contrast enhanced scanning,2 cases showed patchy hyperin-tensity and 2 cases showed branch-like enhancement.In abscess type,6 cases of lesions were wide and 3 cases limited in one quad-rant.Of all 9 cases,there were 3 cases with single abscess and 6 with multiple abscess;and all cases showed hypointensity on T1 WI and hyperintensity on T2 WI with fat suppression.In the enhanced scanning,there were 4 cases with honeycomb-like hyperintensity and 5 cases with annular and patchy enhancement.In mixed type,inflammation was accompanied with abscess and fistula.All cases were hypointensity on T1 WI and hyperintensity on T2 WI with fat suppression.In the enhanced scanning,3 cases showed honeycomb-like enhancement,1 case showed multiple annular enhancement and 1 case showed annular and patchy enhancement.Regions of in-terest were selected and time-signal intensity curves were obtianed in every enhancement cases,all of which were influent.Of all ca-ses,5 showed high-protein deposition in the expansion ducts,exhibiting hyperintensity on T1 WI and hypointensity on T2 WI with fat suppression.Conclusion The abscess type is the most common in PCM and the second is the mixed type.Annular and honeycomb-like enhancement are characteristic appearance of these two types,which are helpful in the diagnosis and classification of PCM.

Objective To translate the English version of the Nursing Profession Self-Efficacy Scale into Chinese,and to test the reliability and validity of the Chinese version. Methods The reliability and validity of the Chinese version of scale was tested among 480 nurses from Tianjin First Central Hospital. Results The revised Chinese version of the Nursing Professional Self-Efficacy Scale contained 19 entries, the Cronbach′s α coefficient was 0.95, the test-retest reliability was 0.91. Conclusions The revised Chinese version of the Nursing Profession Self-Efficacy Scale has acceptable reliability and validity. It can be used to measure the Nursing Profession Self-Efficacy among nurses in China.

Objective To find an ideal method of DNA isolation from blood and especially from clotted blood and to minimize the volume of blood collected for laboratory and clinical tests.Methods DNAs were isolated from antiagglutinated and agglutinated blood samples from auricular veins of 30 healthy subjects. The DNAs of these samples were obtained by a nonenzymatic, nontoxic procedure optimized by us and determinated by agarose gel electrophoesis and PCR. Results The yields of DNA isolated from clotted blood and antiagglutinated blood were (40.2?8.86)mg DNA/L and (39.1?10.2)mg DNA/L, and purities were 1.87?0.11 and 1.92? 0.12. The DNAs that we isolated from all samples had high molecular weight and by PCR the dimorphism of ALU alleles of the 8th intron of t-PA was easy to be obtained, so they were complete and reliable. Conclusion This method is rapid, easy, efficient and nontoxic for isolation of DNA from clotted and fresh blood and meets requirements for clinical testing and molecular biology study.

Objective To explore the effect of modified type B lynch uterine suture combined with calcium in the prevention and treatment for uterine atony postpartum hemorrhage.Methods 63 pregnant women with postpartum hemorrhage risk factors which would be underwent cesarean delivery were randomly divided into the study group (32 cases) and the control group (31 cases).The study group was given intravenous calcium gluconate (10ml) 10 min before the operation,and modified type B lynch uterine suture in the operation.The control group was only given the same dose of oxytocin of the study group.Results The amount of bleeding intraoperation and 24h after the operation was (182.6 ± 47.7) ml and (214.1 ± 63.8) ml,respectively,the time of intraoperative uterine contractions was (3.8 ± 1.3)min,the degree of hemoglobin decreased 24h after the operation was (9.2 ±2.0) min in the study group,which were less than those of the control group(t =7.26,2.97,2.39,4.01,all P ＜ 0.05).There was no statistically significant difference in puerperal morbidity and neonatal asphyxia between the two groups (all P ＞ 0.05).Conclusion The modified type B lynch uterine suture combined with calcium could effectively prevent and treat uterine atony postpartum hemorrhage,and could not increase the rate of neonatal asphyxia and puerperal morbidity.

Objective: To study the current situation and distribution characteristics of pesticide poisoning in Ji'nan area, and to provide the basis for formulating the policy of scientific prevention and control of pesticide poisoning. Methods: The cases of pesticide poisoning from 2012 to 2016 were collected from medical institutions in Ji'nan, and the data was subjected to statistical analysis. Results: From 2012 to 2016 in Ji'nan reported a total of 2 237 cases of pesticide poisoning, non productive pesticide poisoning cases (72.78%, 2 149/2 237) and mortality (17.73%, 381/2 149) was significantly higher than that of productive pesticide poisoning. The average age is 46.78±18.57. The highest mortality rate of pesticide poisoning is more than 70 age group of the non productive pesticide poisoning. Organophosphorus pesticides (67.68%, 1 514/2 237) are the main pesticides causing poisoning, followed by herbicide (23.74%, 531/2 237). The highest mortality rate of pesticide was Paraquat (36.45%, 160/439), the second is the dichlorvos (19.19%, 170/886). Conclusion Pesticide poisoning is a public health problem and social problem which is harmful to the health of the residents in Ji'nan. It is necessary to strengthen the control and management of high toxic pesticides. Pay attention to the psychological intervention of elderly people to reduce the incidence of pesticide poisoning.

Objective To observe the effect of panaxadiol saponins (PDS) on intellectual decline and cerebral lesion induced by destabilization of cervical vertebra in mice.Methods44 mice were randomly divided into the normal control group, model group, high PDS doses group and low PDS doses group with 11 animals in each group. The model of destabilization of cervical vertebra was established by operating and intraperitoneal injection of PDS performed in the PDS high doses group (14 mg/kg) and low doses group (7 mg/kg) once everyday for 50 days. The memory ability of mice was evaluated by the water-maze test and tittup platform experiment. 50 days later, all mice were executed and the activities of superoxide dismutase (SOD), lactic dehydrogenase (LDH) and the content of maleic dialdehyde (MDA), nitric oxide (NO) in encephalon were tested.ResultsIn the PDS groups, the incubation period and error times in water-maze test shortened significantly ( P<0.05), aod the reaction period in tittup platform experiment shortened and wrong times decreased obviously ( P<0.01) compared with the model group. The activities of SOD, LDH increased ( P<0.01), and the content of MDA and NO decreased ( P<0.01, P<0.05) in the PDS group compared with the model group.ConclusionPDS has certain protective and improving effect on the decline of memory ability and cerebral lesion induced by destabilization of cervical vertebra in mice.

BACKGROUND:Twin block appliance has been shown to effectively promote mandibular bone growth of Class Ⅱ malocclusion patients and improve lateral appearance of mandibular retrusion.The other improvement of Twin block appliance on hard-soft tissues remains unclear.OBJECTIVE:To assess the hard-soft tissue changes following Twinblock appliance treatment.METHODS:Atotal of 48 adolescent patients,30 males and 18 females,aged 11.6 years (range 9.58-12.37) with Class Ⅱ malocclusion were selected from Department of Orthodontics,Affiliated Stomatology Hospital of Hebei Medical University.Of them,27 were treated with Twinblock appliance as the treatment group,and 21 adolescent patients without treatments were selected as the control group.Cephalonetric analysis was used to compare the changes before and after treatment.RESULTS AND CONCLUSLON:Comparative analysis showed that Twinblock could effectively adjust or improve the effect of the natural growth,reduced upper lip convexity and mentolabial furrow angle.Moreover,Twinblock inhibited facial vertical distance reduction and facial convexity increase to decrease the potential differences between maxillary and mandibular bones in sagittal plance.Results show that Twinblock could effectively improve the profile of soft-tissues of Class Ⅱ malocclusion cases,

Objective To explore the mechanism of immunomodulatory activity of triptolide on healthy volunteers peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs). Methods Healthy volunteers-derived pDCs were sorted by flow cytometry, then incubated with triptolide (0, 5, 10, 30 μg/L). After 24 hours, we detected the concentration of IFN-α, IL-6, TNF-α using ELISA. After 5 days, the cultrural cells were collected and analyzed by flow cytometry, light microscope and electron microscope scanning. Results Triptolide-treated pDCs secreted lower level of IFN-α,IL-6 ,TNF-α, triptolide could inhibit pDCs differentiation to DCs which displayed more immature morphology and immunophenotypes than untreated-pDCs. Conclusion Triptolide could decrease the immune function of pDCs, inhibit differentiation and maturation of pDCs.