Objective To quantify the effect of rehabilitation training on cardiopulmonary function and the quality of life of patients with coronary heart disease after percutaneous coronary intervention (PCI). Methods Sixty-nine patients after PCI were randomly divided into three groups. Those in group A were given conventional medical therapy; group B had additional walking exercise; group C had both additional respiratory training and the additional walking exercise. Treadmill exercise tests, pulmonary function examinations and a health questionnaire were used to evaluate cardiopulmonary function and quality of life after three months of treatment. Results After three months of treatment cardiopulmonary function and quality of life had improved significantly in groups B and C, and there was significant difference between groups B and C. Conclusion Walking and respiratory function training can improve cardiopulmonary function and quality in life after PCI.

Objective To research and analysis the ocular health condition of Chongqing city inhabitants .Methods We col‐lected and analysised 16 548 ocular health datas of Chongqing city inhabitants that had been to our hospital for taking physical ex‐amination or other non‐ocular diseasea during 2012 to 2014 .Results The prevalence rate of the ocular disease was 22 .92% in Chongqing city inhabitants .The highest prevalence rate was the conjunctivitis among the many diseases ,the second highest one was the dry eye syndrome .Conclusion The ocular health condition of Chongqing city inhabitants still need to be improved .

Juvenile dermatomyositis is a systemic autoimmune disease primarily affecting muscle and skin.The recent researches on proposed criteria based on specific clinical and laboratory features are summarized,recommendations for a new criteria are provided and aggressive treatment approaches,including multiple initial therapies,new immunosuppressive and biological therapies for refractory disease are discussed as well.

The hyper immunoglobulin M syndromes(HIGM) are a heterogeneous group of genetic disorders resulting in defects of immunoglobulin class switch recombination,with or without defects of somatic hypermutation.They can be classified as defects of signalling through CD40 causing combined immunodeficiency,or intrinsic defects in B cells of the mechanism of class switch recombination resulting in a pure humoral immunodeficiency.This review summarizes the molecular pathogenesis of HIGM.

Objective To study the relationship between carotid atherosclerotic plaque and levels of plasma IL-18,CRP,adiponectin(APN)in cerebral infarction patients with diabetes mellitus(DM).Methods Fouty cases of cerebral infarction patients with DM(A group)and 40 case without DM(B group)were randomly selected.All patients with CI were examined on carotid arteries using color Doppler Ultrasonography.Levels of plasma IL-18,CRP and APN were examined and analyzed.Results There were 36 patients(90％)with atberosclerotic plaque and 4 patients(10％)without in group A,among them,28 cases(70％)with vulnerable plaque and 8 cases(20％)with non-vulnerable plaque.There were 26 patients(65％)with atherosclerotic plaque and 14 patients(35％)without in group B,among them,16 cases(61.4％)with vulnerable plaque and 10 cases (38.6％)with non-vulnerable plaque.The level of plasma adiponectin([5.22 ± 1.79]mg/L)in group A was significantly lower than that([7.24 ± 2.31]mg/L)in group B,and there was significant difference between these two groups(t =2.42,P ＜ 0.05).The levels of IL-18([213.5 ± 25.24]μg/L)and CRP([5.42 ± 2.14]mg/L)in group A were obviously higher than those([187.86 ± 23.13]μg/L and[3.57 ± 1.12]mg/L)in group B,and there was significant difference between these two groups(t =5.72,3.551 resectively,P ＜ 0.05)Conclusions Adipenectin might be a protective factor in the development of atherosclemsis in diabetes mellitus.The elevation level of IL-18 and CRP may related with instability of plaque and the occurrence of cerebral infarction.

OBJECTIVE To find out the knowledge and understanding level of prevention from needle-stab for practice nurses to train and educate their professional safety precautions. METHODS There were 105 practice nurses who practiced in the hospital from Jul 2003 to Apr 2004,and were asked for filling out the inquiry paper forms which made by ourselves. RESULTS We found that only 20 nurses were good at understanding their professional safety precautions which occupied 19.05%.Fifty nine nurses understood just so-so which occupied 56.19%.Twenty six nurses were poor-understood which occupied 24.76%. CONCLUSIONS The results are not satisfied with the practice nurses,so we suggested the medical school teachers and the hospital supervisors think highly of training and educating the practice nurses. Let them learn and understand the knowledge of the professional safety precautions.Restricted the training and education plan and countermeasures to avoid the blood infectious diseases.

[Objective]To understand the alienation of contemporary col ege students casual situation and problems, discuss col ege student leisure time value of values education and role. [Method] Horizontal analysis between the U.S. and Chinese universities, col eges and universities within the longitudinal anal-ysis of the current situation of leisure education.[Results] Alienation of contemporary col ege students casual question has three aspects: the lack of leisure and skil s to form a single, low-level leisure and disorganized ,leisure concepts matter of this theory. [Conclusion] Propose to strengthen values education col ege of casual modern higher education development is a necessary requirement for students to achieve an effective way to cultivate harmonious person-ality , is the solution of recreational alienation practical needs of higher education which should not be overlooked as an important part.

Objective To evaluate the efficieency of nursing care for mild Alzheimer's disease.Methods 42 cases of patients with mild Alzheimer'sdisease were randomly divided into 2 groups: the nursing care group and the control group.MMSE and ADL tests were applied at the baseline and after 2 year.Results Cognitive ability and activities of daily living of the nursing care group comparatively maintained than those of the control group after 2 year(P＜0.01).Conclusion Nursing care at early stage may help to delay the progression of Alzheimer's disease.

Cancer can induce venous thromboembolism by many ways. It influences tumor patients life quality and prophylaxis. Venous thromboembolism can also indicate carcinogenesis, so the association between cancer and thrombosis is paid more and more attention. Proper treatment to prevent thrombus can not only reduce compilation, but improve patients prognosis.

Objective To culture human sapovirus(HuSaV) GⅠ.1 in vitro and prepare polyclonal antibody against the capsid protein VP1.Methods HuSaV GⅠ.1 positive stool specimens preserved in diarrhea department of National Institute for Viral Disease Control and Prevention were inoculated with HuTu-80 cells supplemented with different bile acid salts[glycine chenodeoxycholic acid(GCDCA) and glycine cholic acid(GCA)],and the infection,proliferation and passage of the virus were determined by PCR and RT-qPCR.The VP1 gene was amplified by PCR and cloned into prokaryotic expression vector pGEX-6P-1.The constructed recombinant expression plasmid pGEX-6P-1-VP1 was transformed into E.coli BL21(DE3) and induced by IPTG.Two female New Zealand white rabbits were immunized with the purified recombinant VP1 protein for 4 times.The blood samples were collected 18,28,38 and 48 d after immunization,and the serum titers were detected by ELISA.Results HuTu-80 cells were effectively infected by HuSaV GⅠ.1 in the presence of bile acid salt GCA,and the proliferated virus were stably and continuously transmitted for three generations in HuTu-80 cells.The expressed recom-binant GST-VP1 protein showed a relative molecular mass of about 86 000,and about 60 000 after purification(GST tag excision).The titer of polyclonal antibody against HuSaV VP1 protein was over 1:12 800.Conclusion HuSaV was successfully isolated and cultured in vitro using HuTu-80 cells supplemented with bile acid salt,and polyclonal antibody with high titer against HuSaV VP1 protein was prepared,which laid a foundation of in-depth research of HuSaV identification,infection and pathogenesis.