With the development and wide application of antibiotics, the number of resistant strains keeps growing and there is a trend towards a higher isolation rate of methicillin-resistant staphylococcus aureus (MRSA) year by year, accompanied by the increasing drug resistance.As reported in recent years, vancomycin resistant or intermediate staphylococcus aureus was isolated from different geographical regions.The prevalence of MRSA infection has become a serious public health problem, which is also the global focus in the medical field.This review focuses on the prevalence of MRSA colonization and infection, drug resistance mechanism,mode of transmission, prevention and efficacy in neonatal intensive care unit.

Suppurative Samples from 52 CSOM patients were analysed for its e.tiological elements with the methods of aerobic culture, L-phase-bacteria culture and anaerobic culture, the detective rates of which were 76.9%, 76.9% and 23% respectively. There was no significant relationship between the detective rates and clinical types of CSOM. The detective rate of mixed infection was 69%, and simple infection 60.5%, which was significantly lower than those of caries and cholesteatoma, 92.7% (P

The infrared spectra of benzoic acid in its solid form and in various solvents were determined.The result confirmed that the carbonyl bond of the dimer form of benzoic acid occurs at 1694 cm~(-l),but not at 1653 cm~(-1).The influences of hydrogen bond (polar solvent) and solvent effect (nonpolar solvent) on the vibrational frequency of C=O bond was also studied.In the nonpolar solvent,owing to the increment of the dielectric constant (?) of the solvent,C=O stretching frequency decreases slightly and the relationship between them can be expressed with the following formula:Here K and C are two contants,V_v and V_s represent the vibrational frequencyof C=O bond of benzoic acid in vapor and in solvent respectively.

Objective To develop and verify an indirect ELISA method for determination of specific IgG antibody of rhesus monkey serum against SARS-CoV-2 variant strain. Methods An indirect ELISA method for the determination of specific IgG antibody was developed using inactivated SARS-CoV-2 Beta variant strain inactivated vaccine as coating antigen,and optimized for the coating antigen concentration(1,2 and 4 μg/mL),dilution of serum(1∶800～1∶12 800),blocking solution(PBST containing 1% BSA,2% BSA,1% skim milk,2% skim milk and 1% BSA + 1% skim milk),blocking time(30,60 and 90 min),dilution of secondary antibody(1∶5 000,1∶10 000,1∶15 000 and 1∶20 000),incubation time of serum and secondary antibody(30,60 and 90 min),and TMB reaction time(5,10,15,20,25 and 30 min). 60 negative serum samples of rhesus monkeys were detected by the developed method,and the negative and positive critical values were determined. The sensitivity and precision of the methodology were verified. In addition,the specific IgG antibody and neutralizing antibody against SARS-CoV-2 Beta variant strain in 44 serum samples of rhesus monkey were detected by the developed method and microneutralization method,and the correlation and consistency between the two methods were compared. Results The optimum detection conditions were determined:the coating antigen concentration was 1 μg/mL;the blocking solution was PBST containing 1% skim milk,and the blocking time was 30 min;the serum samples to be tested were diluted to 1∶1 600 and incubated for 90 min,and the secondary antibody was diluted to 1∶10 000 and incubated for 30 min;the color development time of substrate was 25 min. The positive critical value and negative critical value of the method was 0. 093 and 0. 084 respectively,and the detection values between them were judged as suspicious. The dilution of5 positive serum samples that showed positive results was 1∶51 200;the coefficients of variation(CVs)of precision were all less than 15%. There was a strong correlation between IgG antibody titer and neutralizing antibody level in the 44 rhesus monkey serum samples(r = 0. 858 0,P < 0. 000 1);the total coincidence rate of the two methods was 90. 9%,the positive coincidence rate was 93. 6%,and the negative coincidence rate was 84. 6%;the consistency test Kappa value was 0. 783 8(95% CI:0. 586 5～0. 981 0). Conclusion The developed indirect ELISA method for eletermination of specific IgG antibody against SARS-CoV-2 Beta variant strain in rhesus monkey serum has good sensitivity and precision,and has strong consistency with microneutralization method,which can be used for the determination of IgG antibody in rhesus monkey serum.

In this work we studied systematically the correlation between the electrone-gativity, n/r or n*/r*, and the vibrational frequency of the chemical bond X-H, vX-H, in the covalent molecular hydrides. The results showed that there is good linear relationship between them.For the molecules of the type HnX-H, two cases were discussed.Case I, when the elements (X)are situated in the same group in the periodic table, the linear relation may be expressed in the following formala:(1)Here K is a constant which varies with the number of valence electrons on the atom X.Case II , when the elements(X) are situated in the same period in the periodic table, the empirical equation can be written as follows:(2)Here A and B are constants determined by the principle quantum number and the number of valence electrons in atom X respectively.For the molecules of the type YnX-H there is also a good linear relationship between vX-H and ∑n*/r*(Y), that is,vX-H?10-2=a + b∑n*/r*(Y)(3)Here a and b are constants determined by the position of the atom(X) in the periodic table.Using the above equations we have calculated the vibrational frequences, vX-H The values obtained are close to those obtained by other authors. Therefore, the results also confirm that the values of electronegativty calculated by our methods published in the previous papers(11) are trustworthy.In addition some characteristics of these equations were analyzed and discussed preliminarily.

Objective To investigate the clinical effect of uterine arterial embolization combined with hysteroscopes in the treatment of cesarean scar pregnancy(CSP).Methods Seventy CSP patients were divided by willingness of patients and their familiality into treatment group and control group with 35 cases each.The treatment group was treated with uterine arterial embolization combined with hysteroscopes,while the control group was treated with uterine curettage after crug therapy.The intraoperative and postoperative situations of two groups were observed.Results The intraoperative blood loss,erythrocyte suspension transfusion,plasma transfusion,preparation time before curettage,hospital stay and pestoperative serum β- human chorionic gonadotropin in treatment group were all obviously lower than those in control group [(420.7 ± 18.9) ml vs.(1893.9 +39.8) ml,(2.1 + 1.3) U vs.(11.1 ±1.4) U,(400.2 ±23.6) ml vs.(1700.3 ± 31.4) ml,(3.9 ± 2.3) d vs.( 18.8 ± 6.7) d,( 14.32 ± 3.89) d vs.(37.83 ± 8.98) d,(312 ± 54) U/L vs.(532 ± 85) U/L,P＜ 0.01].There was 1 case with massive hemorhage in treatment group,while 3 cases in control group.Conclusions Uterine artery embolization combined with hysteroscopes is a safe and effective treatment with less trauma for CSP.It is worthy of clinical promotion and application.

Objective To develop the quality standard for Lajiao Fengshi Plaster. Methods Identification for menthol and borneol were established by gas chromatography SP-2000A. The content of capsaicin, an activity component from capsicum which is the main ingredient of Lajiao Fengshi Plaster, was determined by Kromasil C18 chromatogram columniations(250 mm?4.6 mm, 5 ?m)mobile phase of aqueous acetonitrile-0.17%phosphoric with gradient elution method, velocity of flow: 1.0 ml/min, and detection wavelength: 209 nm. Results The retention time of menthol and borneol is consistent with that of the main ingredient of the reference substance. The good liner range of capsaicin reference substance on calibration curve was 0.03952～0.3952 ?g (r=0.9995), and the average recovery was 96.61%, RSD=0.30%. Conclusion The method is sensitive, specific, and repeatable. It could be used in quality control for Lajiao Fengshi Plaster.

To explore the effect of Ruiqi pill on neuronal calcium level of cerebral cortex in brain of rat embryos.Methods: The cerebral cortex of rat embryos pregnanted for 16-17 days were dissected.The neural cells were divided into Ruiqi pill group and control group randomly after 4-5 days in vitro culture.Effect of Ruiqi pill on neuronal calcium level of primary culture of cortical neurons in rat embryos was measured by laser scanning confocal microscopy.Results:Ruiqi pill could reduce neuronal calcium concentration,no change was found in the control group.Conclusion: Ruiqi pill reduced neuronal calcium concentration,which might be one of the mechanisms of Ruiqi pill in improving memory function.

Objective To analyze the clinical manifestations of placental abruption ,and try to provide evidence‐based data for early diagnosis .Methods Retrospective cases of placental abruption from January 2008 to March 2014 were analyzed ,the clinical characteristics ,etiological factor and outcomes were compared .Results There were 58 cases in Ⅰ degree ,45 cases in Ⅱ degree and 21 cases in Ⅲ degree .The incidence of PIH in Ⅰ degree group was significantly lower than that in Ⅱ degree group ,the incidence of PROM in Ⅰ degree group was significantly higher than that in Ⅱ and Ⅲ degree groups(P< 0 .05) .The main symptoms of Ⅰ degree group were vaginal bleeding .The main performances of Ⅱ and Ⅲ degree group were abdominal pain with or without vaginal bleed‐ing .The cesarean section rate of Ⅰ degree group was significantly lower than that of Ⅲ degree group ,and the rate of maternal‐fetal adverse outcomes was statistically lower than Ⅱ and Ⅲ degree groups (P< 0 .05) .Conclusion It is helpful for early diagnosis of placental abruption and improving prognosis by regular prenatal care ,analyzing and combining with history or risk factors ,ultra‐sound ,physical examination ,and paying attention to clinical performance like abdominal pain and vaginal bleeding ,as well as impro‐ving the ability to identify the abnormal FHR .

Objective To evaluate the short-term effect of open and laparoscopic cholecystectomy surgery on body.Methods Forty patients with cholecystectomy were divided into open surgery group and laparoscopic surgery group by random digits table method with 20 cases each.The clinical index was compared between two groups.The immunoglobulin G(IgG) and procalcitonin (PCT) before surgery and 4 d after surgery were determined.Results The operative blood loss,anal exhaust time,length of stay was (135.80 ±26.74) ml,(1.6 ±0.9) h,(6.7 ±3.2) d in open surgery group,which was better than that in laparoscopic surgery group [(63.41 ± 15.06) ml,(0.4 ± 0.3) h,(2.8 ± 1.9) d],and there was significant difference (P ＜ 0.05).The incidence of heat was 15％(3/20) in open surgery group,which was higher than that in laparoscopic surgery group (0),and there was significant difference (P ＜0.05).There was no significant difference in the IgG and PCT between two groups before surgery (P ＞ 0.05).The IgG and PCT at 4 d after surgery was (13.93 ± 1.98) g/L,(2 153.2 ± 1 452.1) mg/L in open surgery group,which was higher than that in laparoscopic surgery group[(9.23 ± 2.87) g/L,(768.0 ± 237.0) mg/L],and there was significant difference (P ＜ 0.05).Condmion Laparoscopic cholecystectomy has smaller short-term effects on body,while the body of the humoral and cellular immune function in mild,conducive to the body's recovery.