A new photochemical method was employed to cause regional cerebral throm-bosis in the rats by intravenous injection of the rose bengal (1mg/100g body weight)and focal illumination (with a filtered xenon lamp, ?560nm and △?60nm) of the intackskull surface. The regional cerebral blood flow (rCBF), stroke volume (SV), cardiac out-put(CO), heart rate(HR) and the regional blood flow of heart, liver, spleen, kidney andadrenal were used as indexes. The effects of cerebral hemodynamic alterations on cardiacfunction during photochemical reaction were discussed. The results showed that rCBF, SV,CO decreased markedly as compated with the contralateral (P

Waste printed circuit boards(W-PCBs) were multiple smashed and separated, then passed through a 60-mesh screen, treated with hydrochloric acid (2 mol/L), ultrapure water and dehydrated with acetone successively.The filter residue and filter paper were filled into the extraction pool, or inserted into Soxhlet Extraction tube parceled with new filter paper.After addition of 5 μL of internal standard substance, the filter residue above was respectively extracted by Soxhlet Extraction (SE) method or Accelerated Solvent Extraction (ASE) method, cleaned with multi-layer silica gel column and activated-charcoal column to obtained the dioxins samples.The samples were analyzed by isotope dilution high resolution gas chromatography-high resolution mass spectrometry (HRGC-HRMS).The effects of SE and ASE method and number of chlorines atoms on recovery of 15 kinds of 13C-2,3,7,8 PCDD/Fs were investigated, and the accuracy and precision of the two extraction methods were compared.The results show that, the recovery of 15 kinds of 13C-2,3,7,8 PCDD/Fs using ASE method is 54.3%-113.0%, and that of SE is 28.3%-77.7%, and the Toxic Equivalent Quangtity (TEQ) in W-PCBs is 0.075 ng/kg (TEQ) and 0.266 ng/kg (TEQ) using ASE and SE method respectively.Under the premise that accuracy and precision meet with the international standard, ASE is simple, rapid, solvent-free and accurate.

Objective To observe the effect of acupuncture at lumbar Jiaji (EX-B 2) points plus botulinum toxin injection on the seated function of patients with spastic cerebral palsy.Method Sixty patients aged 1-4 years old with spastic cerebral palsy were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by acupuncture at lumbar Jiaji (EX-B 2) points plus botulinum toxin injection, while the control group was by conventional acupuncture plus botulinum toxin injection. After 3 treatment courses, the seated function zone (zone B) of Gross Motor Function Measure (GMFM) was used for evaluation, and the clinical efficacies were compared.Result Respectively after 1, 2, and 3 treatment courses, the total effective rates were 46.7%, 73.3% and 83.3% in the treatment group, versus 33.3%, 46.7% and 60.0%. There were significant differences in comparing the total effective rate between the two groups after 2 and 3 courses (P<0.05). After the first treatment course, the seated function score of GMFM was significantly changed in both groups (P<0.01). After the second course, the seated function score was significantly different from that after the first course in both groups (P<0.01). After the third course, the seated function score was significantly different from that after the second course in both groups (P<0.01). There was a significant between-group difference in comparing the changes of the seated function score during the second treatment course (P<0.01). There was a significant between-group difference in comparing the changes of the seated function score during the second treatment course (P<0.05). Conclusion Acupuncture at lumbar Jiaji (EX-B 2) points plus botulinum toxin injection can produce a more significant efficacy in improving the seated function of patients with spastic cerebral palsy.

Objective:To investigate the effect of restraint stress on T, B lymphocyte transformation rate and phagocytic function of peritoneal macrophage.Method:Cells were extracted from the abdominal cavity of mice with S180 tumor and then were injected subcutaneously in the right oxter in 0.2 ml/mouse. Some of the mice were bound at the same time. There were four groups in all, they were normal control, tumor mice without restrain, restrained mice without tumor, and mice with both tumor cell injection and restrain. All mice were killed after 10 days, T and B lymphocyte transformation rates of spleen were examined, the phagocytic function of peritoneal macrophage was also tested. The weights of tumors were also recorded.Result:Compared with tumor mice without restrain, the mice with both tumor injection and restrain had lower T, B lymphocyte transformation rates and lower phagocytic function of peritoneal macrophage. The weight of tumor in the study group was also higher than that of tumor mice without restrain.Conclusion:Restraint stress aggravates the depression of the immune function of mice with S180 tumor and promotes the growth of the tumor.

Objective To investigate the clinical significance of antiendothelial cell antibodies (AECA) in systemic vasculitis. Method With Human umbilical vein endothelial cell (HUVEC) as substrate cell, sera from 129 systemic vasculitis patients [including 59 Behcet′s disease(BD), 28 Takayasu arteritis (TA), 20 Wegener′s granulomatosis (WG), 8 polyarteritis nodosa (PAN), 9 microscopic polyangiitis (MPA), 5 Churg-Strauss syndrome (CSS)] were screened for the presence of AECA by ELISA. Sera from SLE, RA and healthy donors were examined as controls. The association of AECA to clinical disease activity was analyzed. Result The prevalence of AECA by HUVEC cell-ELISA was 59% in systemic vasculitis [48% in BD,79% in TA, 65% in WG, 63% in PAN, 44% in MPA, 80% in CSS], 46% in SLE, 4% in RA, and 2.4% in control group. Compared with patients with RA and control group, AECA were more frequently found in patients with systemic vasculitis and SLE (P

Objective Mongolian gerbil can make themself urine concentration for saving water and adapt to the harsh desert environment, due to their very unique moisture control system in the body.Methods Mongolian gerbil is resistant to drought on account of their special kidney. Histology of the kidney, ureter and bladder in Meriones Unguiculataus were observed by light microscopy using HE staining.Results The results showed that compared with rats and mice, the Mongolian gerbils have more developed distal tubules, and well developed inner renal medulla.Conclusions We hope that the findings of this study enrich our understanding of the histology of urinary system in Mongolian gerbils and provide support for the laboratory animalization of this animal.

Objective To approach effect of Yiqihuoxue decoction on remnant stomach paralysis syndrome after subtotal gastrectomy. Methods A total of 44 patient of remnant stomach paralysis syndrome were randomly recruited into a control group and a treatment group, with 22 patients in each group. The control group was treated with TPN parenteral alimentation. On this basis, the treatment group was treated with Yiqihuoxue decoction and mental intervention. The clinical effects were observed in both groups after the treatment. Results The total effective rate was 54.55% and 100% in the control group and the treatment group respectively, showing statistic difference(P=0.00625 ＜0.01).Conclusion It was effective to treat remnant stomach paralysis syndrome with Yiqihuoxue decoction, TPN parenteral nutrition and mental intervention.

Objective To investigate the difference of impulsiveness between patients of obsessivecompulsive disorder(OCD) and healthy controls.Methods Seventy-one patients who met the DSM-Ⅳ criteria for OCD,and sixty-five healthy controls were recruited.Impulsiveness and the severity of symptom were assessed with Barratt Impulsivity Scale (BIS-11) and Yale-Brown Obsessive Compulsive Scale (Y-BOCS) before and the end of 16th week treatment.Results Before treatment,attentional impulsivity scores(14(12,16)) and non-planning impulsivity scores (26 (23,28)) were significandy higher (all P＜ 0.01) in OCD patients compared with controls (12 (11,13),23 (20.5,26)).Mter treament,the reduction rate of attentional impulsivity was positively correlated with the reduction rate of Y-BOCS (r=0.443,P=0.018).Logistic regression analysis reported that with the total scores of BIS-11 increased,the effect of treatment was improved (β=-0.149,P=0.002).Conclusion Increased attentional and non-planning impulsivity are found in OCD.As the improvement of symptom,the scores of attentional impulsivity are also decreased.The high total scores of BIS-11 are correlated with the efficacy of treatment.

Objective To observe the changes of ischemia modified albumin (IMA),high -sensitivity C -reactive protein (hs -CRP)after percutaneous coronary intervention (PCI).The factors related to intervention procedures to patients with IMA and hs -CRP elevation after PCI and impact of the risk of IMA and hs -CRP on the clinical outcomes were investigated.Methods IMA,hs -CRP from vein blood of 90 cases received PCI were detec-ted before operation and after operation at time of 30 min,2h,6h and 24h.The clinical characteristics of patients and factors related to intervention procedures were recorded.The number of lesion vessels and the relative factors in PCI were compared.The clinical outcomes of the patients were followed up.Results After operation,IMA was significantly different compared with that before operation (t =2.293,P <0.05).The concentration of IMA peaked at 2h after operation,which was significantly different from that before surgery (t =1.116,P <0.01).The increase of IMA and hs -CRP were closely related with the inflation pressure,total times of inflation,dilated times,length and diameter of stent and the number of stents (t =3.678,P <0.01 ).The angina onset was more common and length of stay was longer in the patients with the rise of IMA and hs -CRP.Conclusion PCI may cause myocardial injury,which is associated to inflation pressure,total time of inflation,dilated times,length and diameter of stent and the number of stents.To a certain extent the level of troponin can predict the outcomes.

Objective To investigate the effect of hydrogen sulfide (H 2 S or NaHS)on myo-cardial ischemia reperfusion injury induced in type 2 diabetic rats in vivo and the role of AMP-activated protein kinase (AMPK)signal pathway.Methods The induced type 2 diabetic rat models were anesthetized,left thoracotomy were performed.All the models were randomly divided into six groups (n = 14):group Sham;group IR:the left anterior descending artery was ligated 30 min, reperfused for 4 hours;group CC:prior to thoracotomy,compound c was intraperitoneally injected 250 μg/kg,then received the same treatment as group IR;group DMSO received the same treatment as compound c group but DMSO was injected intraperitoneally as control;group NaHS:the left ante-rior descending artery was injected NaHS 0.05 mg/kg then reperfused for 4 hours;group CC +NaHS:prior to thoracotomy,compound c was intraperitoneally injected 250 μg/kg,then NaHS 0.05 mg/kg injected intravenously and reperfused 4 hours.All the rat models euthanatized,infarcted area was detected by TTC assay.The AMPK,LC3 and p62 were analyzed by Western blot.Results Com-pared with group Sham,the infarcted area and concentration of AMPK,LC3 and p62 were increased in other groups (P <0.05).Compared with group IR,the infarcted area and concentration of LC3, p62 markablely decreased in group NaHS (P < 0.05 ).Compared with group NaHS,the infarcted area and concentration of LC3,p62 significantly increased but AMPK down-regulated in group CC+NaHS (P <0.05).Conclusion Hydrogen sulfide could alleviate myocardial infraction via AMPK sig-nal pathway in type 2 diabetic rats'IR models.