OBJECTIVE:To establish the methods for content determination of lead and cadmium in Achyranthes bidentata from different habitats.METHODS:Graphite furnace atomic absorption spectrometry was applied.RESULTS:There were a good linear relationship between the concentration and absorbance of lead and cadmium.The linear range of lead were 0～80.00 ?g?L-1(r=0.999 6) with recovery of 95%～101%(RSD ranged from 0% to 5.00%,n=10).The linear range of cadmium were 0～7.000 ?g?L-1(r=0.998 2) with recovery of 98%～103%(RSD ranged from 1.00% to 5.00%,n=10).CONCLUSION:The method is simple,accurate,convenient and sensitive and the results are satisfactory.

Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g～325?g for imperatorin and in the range of 50?g～250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.

Objective To study the optimum inclusion process for volatile oil from Tongketing capsules. Methods The study was carried out with orthogonal test at different levels. The utilization ratio of volatile oil was used as the index to optimize the inclusion process. Results The optimum preparation conditions for inclusion were: oil: ?-cyclodextrin: water was 1: 4: 40 (V: V: V). The inclusion time was 10rain and inclusion complexes was dried at 70℃. Conclusion This optimized process is simple, efficient and can be used for the production of Tongketing Capsule on a large scale.

OBJECTIVE:To investigate the cognition of clinical pharmacists on the clinical pharmacy practice and their profes-sion,and provide reference for further developing clinical pharmacy practice. METHODS:A questionnaire was designed,and ran-dom sample was conducted for the clinical pharmacists from 30 secondary and tertiary hospitals in Shanghai,and the investigation results were analyzed statistically. RESULTS:Totally 130 questionnaires were sent out,and 102 were effectively received with valid response rate of 78.46%. 94.11% of the investigated subjects were willing to be a clinical pharmacist,but 17.65% of them consid-ered that they were incompetent for their work;29.41% of them thought the current situation of clinical pharmacy practice was not ideal;all the respondents considered that the clinical pharmacist system should be implemented in medical and health institu-tions;the cognition and evaluation of investigated subjects in tertiary hospitals on clinical pharmacy practice and their profession was generally higher than those in secondary hospitals(P<0.05). CONCLUSIONS:The clinical pharmacists in medical and health institutions in Shanghai showed high cognition on their profession,and the clinical pharmacy practice has already made some achievements,but many work still need to be improved. Therefore,clinical pharmacists should continuously study their profession-al knowledge and improve their professional skills and quality;hospitals and universities should strengthen the subject construction and personnel training of clinical pharmacy and deeply carry out the clinical pharmacist system;health administration departments should strengthen the related laws and regulations of clinical pharmacy;colleges and universities should strengthen the discipline construction and personnel training.

Objective:To regulate operation requirement for electric instrument using in surgery and eliminate hidden danger so as to ensure their safety in using.Methods: Through discussed the regulation of operative process for electric instrument using in surgery to develop a insulation detector which can adapt the particular case of hospital, and then to carry out relevant management for this kind of electric instrument.Results: The simplest insulation monitoring method got best reliable data, and it provide accuracy basis for clinical electric instrument. Therefore, it ensured the safety of clinical application of electric instrument.Conclusion: The special insulation monitor detects electric instrument in surgery has series of merits including low investment, fast detection and reliable performance, it not only can ensure the safety of surgery but also can provide traceable basis for preventing relevant medical dispute.

AIM: To establish the determination of baicalin and wogonin in Chantui Zhike Granules (Periostracum Cicadae, Radix Scutellariae, etc.) by HPLC. METHODS: The determination was carried out by HPLC. The mobile phase was acetonitrile 0.6% phosphoric acid(45∶55) and UV detection wavelength was set at 275nm. The flow rate was 1.0mL?min -1 . The column temperature was at 40 ?C . RESULTS: The contents of baicalin and wogonin in Chantui Zhike Granules could be determined by HPLC. There was a good linearity between the absorption peak area and the concentration in the range of 0.104?g～0.52?g for baicalin and in the range of 0.03?g～0.15?g for wogonin, respectively. The average recoveries were 100.69%( RSD =1.75%) and 98.8%( RSD =1.01%) respectively. CONCLUSION: The method is simple, accurate and can be used to determine the contents of baicalin and wogonin in Chantui Zhike Granules.

With ever-increasing knowledge of biomechanics and anatomy of pelvis,more and more findings have been made on the integrated sacroiliac joint system.The sacroiliac complex plays a very important role i n the functions of pelvis due to its specia l biomechanical features.The sacro iliac screw internal fixation is bio mechanically superior to other fixation methods.[

Objective To investigate the diagnostic value of intraductal ultrasonography (IDUS) for bile duct stones by comparing between magnetic resonance cholangiopancreatography ( MRCP), endoscopic retrograde cholangiography (ERC) and IDUS. Methods ERC was performed in 30 patients with suspected extrahepatic cholelithiotic after MRCP. IDUS was applied through guide wire after ERC, and stones were removed if necessary. Results MRCP detected bile duct stones in 22 cases, misdiagnosed bile duct floc as stones in 2 and missed stones in 2 others. The accuracy, sensitivity and specificity of MRCP in diagnosis of bile duct stone were 86. 7% (26/30), 91. 7% (22/24), and 66. 7% (4/6), respectively. ERC detected bile duct stones in 23 cases, misdiagnosed bile duct air bubble as stones in 2 and missed stones in 1. The accuracy , sensitivity and specificity of MRCP in diagnosis of bile duct stone were 90% (27/30), 92% (23/25) and 66.7% (4/6) , respectively. IDUS detected 24 cases of bile duct stones, 4 bile duct floc and 2 air bubble without any misdiagnosis. The accuracy, sensitivity and specificity of IDUS in diagnosis of bile duct stone were 100%. Conclusion IDUS, safe and reliable, is superior to ERC and MRCP in diagnosis of bile duct stone.

Objective To observe the effects of soybean isoflavone (SI) on the action potential of guinea pig papillary muscle and the sodium current in cultured neonatal rat cardiomyocyte.Methods The action potential of guinea pig papillary muscle was measured by glass suspension electrode and the sodium current in cultured neonatal rat cardiomyocyte was measured by whole cell patch-clamp technique. Results The SI reduced the action potential amplitude (APA) and shortened the 50% and 90% of duration of action potential (APD50 and APD90). The SI 0.1μg/ml inhibited sodium current in cardiomyocyte at a inhibition rate of 14.39 %, and the corresponding data of SI 1.0μg/ml at a inhibition rate of 23.48%. Conclusions The SI can reduce the APD of myocardiocyte and has a negative inotropic action on myocardium. These results indicate that SI inhibition of the sodium current may be one of mechanisms of its antiarrhythmic action.

Objective To explore the effect of the transplantation of intact follicle by punching with syringe needle on defect eyebrow reconstruction.Methods Fifty-two patients with eyebrow defects due to burn,trauma,resection and inappropriate eyebrow trimming were recruited,35 with bilateral defects and 17 with unilateral defect.The scalps strips from the safe donor area or temporal region were taken and made into 1 to 2 cm long hair with intact follicle under 3 fold magnifier.The recipient area was punched with 21G or 22G syringe needle.Then the hair shaft was clamped with mi croforceps and the follicles were transplanted to the defective area.Results Folliculitis were found in two burn patients after the transplantation and cured by alcohol inunction.Hairs of the rest patients transplanted grew well.The direction and appearance were satisfied.The survival rate of hair was more than 90 ％.Conclusions It is feasible to restore the partial eyebrow defect by intact follicular transplantation by punching with syringe needle and clamping the hair shaft with microforceps.