Objective To investigate the relationship between PCNA and nm23 expression in breast cancer tissue and its prognosis. Methods Detected the expression of PCNA and nm23 of 278 cases of breast cancer by immunohistochemical technique S-P and analyzed it combined with the clinicopathology. Results PCNA positive rate was 37.4%(104/278),nm23 positive rate was 59.7%(166/278).PCNA and nm23 genes expression had no obvious correlation with the breast cancer pathological type,patients age and clinical stage (P > 0.05). PCNA expression had the positive correlation with the axillary lymph nodes meta-sitasis but negative to 5-year survival rates(P < 0.01 ),nm23 expression had the negative correlation with the axillary lymph nodes metasitasis but positive to 5-year survival rates(P < 0.01 ). PCNA and nm23 expression had the obvious negative correlation between them(P < 0.01 ). Conclusion Combined detection the expres-sion of PCNA and nm23 in breast cancer tissue will be important reference to forcast and evaluate the prognosis of the breast cancer patients.

Background and purpose: MicroRNAs are 19–25-nucleotide noncoding RNA molecules that regulate gene expression at the level of transcription and translation. The study aimed to confirm whether miR-216a suppresses cell proliferation and invasion by targeting PRKCA, thus to reveal molecular mechanism that miR-216a functions as a tumor suppressor in glioma. Methods: PRKCA 3’ untranslated region (UTR)-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-216a on luciferase activity. U251 cells were transfected with miR-216a mimics, and next Western blotting was performed to detect the expression of PRKCA protein. The effects of PRKCA downregulation on cell proliferation and invasion were observed after PRKCA siRNA was transfected into U251 cells. U251 cell proliferation assays were performed when cotransfected with miR-216a mimics. Results:The result demonstrated miR-216a could bind to the 3’UTR of PRKCA and inhibited the luciferase activity by 41%. PRKCA protein expressions were significantly down-regulated when miR-216a was overexpressed in U251. siRNA-mediated downregulation of PRKCA could suppress the potentials of cell proliferation and invasion. Conclusion:miR-216a suppresses cell proliferation and invasion by targeting PRKCA in glioma.

Objective To study the effect of transsphenoidal approach operation for pituitary adenoma.Methods Transoral and transsphenoidal approach operations were performed in 17 patients with pituitary adenoma,and transnosal and transsphenoidal approach operations were performed in 31 patients with pituitary adenoma,from January 2000 to December 2005.For transoral and transsphenoidal approach operations,the upper lip mucous membrane was opened to introduce into the nasal cavity.For transnosal and transsphenoidal approach operations,the instruments were inserted by way of the right nostril,and the nasal septum was opened.During both of the approaches,a tunnel was established between both sides of the nasal septum,through which the sphenoidal sinus was opened,for entering the sella through the bottom.Results Total resection was completed in 37 patients,subtotal resection was completed in 6,and partial resection,in 5.No surgical-related death occurred.Postoperatively,transient hyperuresis was found in 15 patients,and cerebrospinal fluid rhinorrhea was seen in 7 patients.Seventeen patients were followed for 3～24 months(mean,12 months).Recurrence was encountered in 4 patients at 6 months after operation.Four patients were pregnant and delivered of a child.Conclusions For pituitay adenoma not obviously extending to the parasellar,transsphenoidal approach is a safe and effective option.

Objective To construct a RhoC-siRNA expression vector, and study its role on the biological behaviors of hepatoma carcinoma cells. Methods RhoC-siRNA gene was synthesized and cloned into the expression vector pSilencer2.1. The constructed RhoC-siRNA expression vector was stably transfected into hepatoma carcinoma cell line SK-Hep1. The inhibitory effect of RhoC-siRNA on the expression of RhoC in transfected cells was detected by Western blotting. The morphous, growth velocity and the ability of cell adhesion, cell migration, cell invasion before and after transfection was observed. Results Enzyme digestion and DNA sequencing confirmed that the RhoC specific siRNA expression vector was constructed successfully. After transfection, RhoC expression was inhibited by 60%, and no marked difference was observed in cellular morphous and growth curve, while the ability of cell adhesion, cell migration, and cell invasion were markedly decreased. Conclusion The RhoC-siRNA expression vector can effectively suppress RhoC expression in transfected hepatoma carcinoma cells. Although having no effect on the morphous and growth velocity of hepatoma carcinoma cells, it decreases the potentiality of cell invasion and cell metastasis, which may provide a novel applicable strategy for gene therapy on hepatocellular carcinoma.

Objective To establish a subcutaneous and orthotopic transplatation tumor model of human esophageal cancer in nude mice .Methods Fresh tissues of human esophageal cancer were collected and transplanted into subcutaneous places of nude mice .The successfully transplanted primary generation tumor was passed down three times .Then tumor were implanted into the subcutaneous and esophageal submucous tissues of nude mice .The formation rate ,morphology ,invasion and metastasis of tumor were observed .Results The tumor formation rate of subcutaneous transplatation model was 60% ,it was low grade squamous cell carcinoma without lymph node and distant organ metastasis .The tumor formation rate of orthotopic transplatation model was 80% , the morphological characteristics were consistent with human original tumor .Lymph node and organ metastasis could be found ex-tensive .Conclusion We preliminary established effective subcutaneous and orthotopic transplatation tumor model of human esopha-geal cancer in nude mice .It might be an available model for further research of esophageal cancer .

Objective: To know the psychological health status of the college students with guarded experience and to supply reasonable suggestion for psychological health education and consultation.Methods: SCL-90 questionairs were applied to survey the psychological health status of the college students in grade 2 and 3.Results: The psychological characteristic of the students with guarded experience involve.(1) Compared with the national college students norm,the scores of interpersonal sensitivity,depression anxiety and horror are higher(.2) Compared with the college students without guarded experience,the scores of anxiety and horror are higher.(3) Inside the students with guarded experience,the scores of interpersonal sensitivity,depression,anxiety and horror in females are higher than those in males.The above outlined have statistical significance.Conclusion: The guarded experience has positive influence on college students,psychological health,especially on females,We should enforce psychological health education,improve the psychological quality and reduce the psychological disease.

OBJECTIVE To explore how to realize the superior effect of Hospital Information System(HIS) in management of Nosocomial Infection(NI).METHODS Based on the platform of HIS,the prospective investigation,prevalence surveying,targeted surveillance and usage of antibiotics were performed.Drug-resistance was also monitored in real time.RESULTS Applying the HIS,infection control personnel could perform the overall surveillance on patients′ nosocomial infections.The hospital infection control process was optimized and time was saved for all medical personnel.CONCLUSIONS The application of the HIS enhances the hospital infection management level and decreases the incidence rate of NI,which may deserve to be generalized.

Objective:To observe the effect of Governor Vessel-unblocking and mind-refreshing needling technique on diffusion tensor imaging (DTI) in cerebral palsy (CP) children with periventricular leukomalacia (PVL) and investigate the underlying mechanism.
Methods:A total of 18 CP children with PVL received modern rehabilitation training and scalp acupuncture. The before-after study was conducted to compare the fractional anisotropy (FA) of the white matter fiber tracts, gross motor function measure (GMFM) score, Gesell developmental scale and changes in muscle tone.
Results:For the comparison before and after the first course of treatment, there were no statistical differences in DTI findings and FA values (both P>0.05); there were statistical differences in GMFM dimensions A and B (bothP<0.05) and no statistical differences in dimensions C, D and E (allP>0.05); and there were statistical differences in six items of Gesell development test (P<0.01 orP<0.05). For the comparison before and after two courses of treatment, there were statistical differences in DTI findings and FA values in 8 cases between before and after 30 and 60 treatments (bothP<0.05); there were statistical differences in GMFM dimensions A, B and C between before and after 30 and 60 treatments and between 30 and 60 treatments (allP<0.05); there were no statistical differences in GMFM dimensions D and E (allP>0.05); and there were statistical differences in six items of Gesell developmental test between before, after 30 and 60 treatments and between 30 and 60 treatments (P<0.05 or P<0.01).
Conclusion:The Governor Vessel-unblocking and mind-refreshing scalp acupuncture has no significant efficacy on the repair and regeneration of white matter fibers in children with PVL and CP in a short period of time; however, it gradually works along with the prolonged treatment time. In addition, it can improve the motor function and mental development and the efficacy is closely associated with the time of treatment.

Objective: To observe the clinical effect of acupuncture plus occupation therapy for fine motor functions in children with spastic cerebral palsy. Methods:A total of 80 cerebral palsy kids with fine motor dysfunction were allocated into two groups by envelop, 40 cases in each group. Cases in the control group were treated with occupation therapy. Based on the therapy given to the control group, cases in the treatment group were supplemented with acupuncture at the points of the three yang meridians of hand. The treatment was done every other day and 10 times for a course. There was a 15-day interval between two courses, and 3 courses in total. The therapeutic efficacies were evaluated using Peabody developmental motor scale 2 (PDMS-2) and modified Ashworth scale (MAS) before and after treatment. Results:After treatment, the standard score for grasping (Grs), standard score for visual-motor integration (Vis), fine motor quotient (FMQ) and modified Ashworth scale (MAS) were significantly improved in both groups (P<0.05). The improvement in the treatment group was more obvious than that in the control group (P<0.01). Conclusion:Acupuncture plus occupation therapy can achieve better effect than occupation therapy alone in improving fine motor functions of upper limbs in cerebral palsy kids.

Objective:To observe the clinical effect of Nie-pinching spine coupled with point application and injection for cerebral palsy (CP) in children with spleen deficiency.
Methods:A total of 70 CP children with spleen deficiency were randomly allocated into a treatment group (n=35) and a control group (n=35). Children in the treatment group were treated with comprehensive rehabilitation training, Nie-pinching spine, point application and point injection, whereas children in the control group were treated with comprehensive rehabilitation training and oral administration of multi-enzyme tablets. One course of treatment made up of 20 d. There was a 20-day interval between two courses. The cases were treated for a total of 3 courses. Then the therapeutic efficacies were evaluated using scores of spleen-deficiency symptoms and gross motor function measure (GMFM).
Results:After 3 courses of treatment, the total effective rates for spleen-deficiency symptoms and rehabilitation were 85.7% and 77.1% respectively in the treatment group, versus 51.4% and 57.1% respectively in the control group, showing statistical differences (bothP<0.05).
Conclusion:In addition to comprehensive rehabilitation training, Nie-pinching spine combined with point application and injection obtained better effects than oral administration of multi-enzyme tablets.