[Objective]To study the role and the effect of establishing the plan of meeting emergency in the rehabilitation and reestablishment of high-octane body hurt.[Method]Eighty six patients of high-octane body hurt were collected from Shi He Zi people's hospital during 1998 to 2007.According to Gustilo-Anderson categorize of open fracture and mark of ISS,high-octane body hurt can be divided into three parts.Meanwhile,different part can be taken different methods to rehabilitate and reestablish.Therefore,we can analyze pertinence between cure principle and curative effect.[Result]Eighty three patients were observed during the period of fifteen and thirty-three months,the average were nineteen months.Five of them contracted double floating knee injury,and their knee joint appeared anchylosis,abnormality and function failure afterfracture healed up.We did one-off knee joint release while extracting internal fixation,and used CPM machine to assist exercise.Fortunately,the function of knee joint could be renewed.Another five patients' fracture did not heal up very well.However,after experiencing the apply of oneself bone autograft,fracture could be healed up perfectly.The time of allograft bone union ranged from five to sixteen months,the average time were 7.5 months.There are three patients contract paraplegia,and three patients dead.By autopsy,some of them belongs to multiple fracture and multiple viscera burst.Mortality was 3.48%.[Conclusion]By establishing the plan of meeting emergency of high-octane body hurt,using the Gustilo-Anderson categorize of open fracture and mark of ISS,we can systemic classify damage degree,and make scientific treatment by stages,therefore,attaining our goal of early diagnoses,early treatment,and decreasing mortality and decreasing the proportion of disabled.After all,the plan of meeting emergency plays an essential role in traumatie surgery.

[Objective]To detect the expression of tumor necrosis factor-?(TNF-?)and interleukin-18(IL-18)protein in herniated intervertebral disc,and discuss the role of TNF-? and IL-18 protein in the course of the intervertebral disc degeneration and to clarify the mechanism of the lumbar intervertebral disc degeneration and herniation. [Method]Forty nine specimens were studied from December 2005 to April 2007 in the First Affiliated Hospital of Shihezi Medical College.Experimental group contained thirty-four herniated intervertebral disc and control group contained fifteen discs,which were collected from the patients with spine fracture or autopsy.The specimens were divided into two parts,one part was fixed in 10% formalin about 24 hours and was embedded in paraffin.Four ?m serial sections and S-P method were used.The sections were stained with TNF-? and IL-18 monoclonal antibodies,and the results were observed by OLYMPUS microscope.Another part was changed into well-distributed liquid,which was determined by Elisa method respectively,in order to detect density of TNF-? and IL-18 protein.Software SPSS 13.0 was used for statistical analysis.[Result]1 TNF-? and IL-18 protein were expressed mostly in cytoplasma.The expression of IL-18 in the experiment group was obviously higher than in the control group.There were statistical significances(P

Objective To assess the value of ECG in the diagnosis of acute pulmonary embolism (APE). Methods Twenty-eight patients with APE were included,with mean age of 56?16,and PaO 2,electrocardiogram (ECG),echocardiogram (ECHO) and ventilation/perfusion lung scan were performed. Results 96.4% patients had risk factors,and had different degree of hypoxia. 92.9% patients had ECG changes,most of whom (57.1%) showed ST-T changes. The ventilation/perfusion lung scan confirmed the diagnosis. Conclusion Considering the clinical symptom,arterial blood gas analysis,the early changes of ECG are useful in the diagnosis of the APE.

Objective To differential the biologic characteristics and multiple differentiation potential of mesenchymal stem cells in nucleus pulposus in (NP-MSC) scoliosis patient and patient with degenerative interverthral disc.Methods The human nucleus pulposus-mesenchymal stem cells were isolated and cultured with enzyme digestion from 2 patients of scoliosis and 2 patient with degenerative intervertbral disc separately.Cellular proliferation was detected with MTT assay and trypan blue.The immunophenotype expression of NP-MSC was detected by flow cytometry in scoliosis and degenerative group.The multiple differentiation ability of cells was assessed respectively using alizarin red dye,Oil red O dye and immunohistochemical staining.Results The primary cell morphology of scoliosis NP-MSC was the shape of spindle,while the degenerative NP-MSC was inhomogeneous.However,both of them became spindle shape after passages.The scoliosis NP-MSC was stronger than degenerative one in metabolic activity and proliferation.The percentage of positive antigen expression of CD44,CD105 and CD29 was 97％-100％ in scoliosis NP-MSC group,but 88.7％-97％ in the degenerative group.The expression of mature cell marker CD24 was negative in both groups.Furthermore,the MSC i.isolated from both groups differentiated along the osteogenic,chondrogenic but not adipogenic lineages.Conclusion The scoliosis and degenerative NP contains mesenchymal stem cells.Moreover the scoliosis NP-MSC had stronger ability of cell metabolic activity and proliferation.These cells have multiple differentiation potential with the exception of their adipogenic differentiation ability.

BACKGROUND:Deluxe-PS knee prosthesis is designed based on the anatomical characteristics of the Chinese peoples’ knee joint, especial y the morphological features of femoral condyle. The distance between median and lateral knee prosthesis femoral condyle is less than that of the imported prosthesis for 3.5 mm.
OBJECTIVE:To explore the short-term efficacy of Deluxe-ps knee prosthesis in simultaneous bilateral total knee arthroplasty.
METHODS:Fifteen knee osteoarthritis patients (30 knees) were included as the experimental group, and treated with simultaneous bilateral knee arthroplasty by Deluxe-ps knee prosthesis. Twenty patients (40 knees) in the control group were treated with simultaneous bilateral knee arthroplasty by PFCSigma knee prosthesis. The knee society score, hospital for special surgery knee score and knee joint range of motion were compared between two groups;the operation time and the intraoperative blood loss were compared between two groups.
RESULTS AND CONCLUSION:The patients in both groups were fol owed-up for 12 to 24 months, with an average of 16 months. Postoperative knee pain was relieved, and the joint function was recovered satisfactorily. The knee society score, hospital for special surgery knee score and knee joint range of motion in two groups were improved significantly after treatment (P<0.05). There were no significant differences in postoperative knee society score, hospital for special surgery knee score and knee joint range of motion between two groups (P>0.05), and there was no significant difference in intraoperative blood loss between two groups (P>0.05), while there was significant difference in operation time between two groups (P<0.05). The results showed that the short-term effect of Deluxe-ps knee prosthesis in simultaneous bilateral total knee arthroplasty was satisfied.

Objective To investigate the serum C-type lectin domain family 4 member G(CLEC4G)level and its clinical value in patients with Atopic Dermatitis(AD).Methods The blood samples of 60 AD patients and 29 control patients were collected,and CLEC4G,Interleukin-33(IL-33),total immunoglobulin E(tIgE),specific IgE(specific IgE),and eosinophil levels were detected.The correlation between CLEC4G level and clinical data of AD patients and IL-33 was analyzed.The risk of AD was evaluated by Logistic regression analysis of CLEC4G,IL-33 and other indicators.Results Compared with the control group,the serum CLEC4G level in AD patients was significantly decreased(359.4±57.3 vs.521.8±48.1)pg/mL.There was no significant difference in CLEC4G level between child-hood,adolescent and adult,male and female AD patients.Compared with tIgE≤100 kU/L group,CLEC4G level was significantly decreased in 100～200 kU/L group and tIgE≥200 kU/L group,but there was no significant difference between 100～200 kU/L group and tIgE≥200 kU/L group.Serum CLEC4G level decreased significantly only in the moderate AD group,but had no significant difference among the other groups.The serum level of IL-33 was increased in AD patients,but there was no significant correlation between CLEC4G and IL-33(r = 0.090,P = 0.495).Age less than 14 years old and IL-33 were risk factors for the incidence of AD,with OR values of 2.756 and 1.241,95%CI of 1.076～7.060 and 1.030～1.495,respectively.CLEC4G was a protective factor for AD(OR = 0.890,95%CI:0.809～0.979).Conclusion CLEC4G may be a protective factor independent of IL-33 mediated AD pathogenesis.