1.Direct activation of expression vector withβ-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts
Wenhui PI ; Long LIANG ; Hong TANG ; Yiyuan ZHANG ; Yanhua GUO ; Limin WANG ; Chunhe XIANG ; Ping ZHOU ; Shouren LIU
Acta Laboratorium Animalis Scientia Sinica 2014;(5):13-16
Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.
Result Analysis
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