Objective To explore the clinical effect of periodontic treatment in type 2 diabetic patients (T2DM ) with chronic periodontitis on basis of hypoglycemic therapy. Methods From September 2011 to January 2014 ,120 T2DM patients with chronic periodontitis were selected and divided into treatment group and control group with 60 cases in each group. Patients in control group were treated with conventional hypoglycemic therapy. Patients in treatment group were treated with initial periodontal therapy additionally on basis of conventional hypoglycemic therapy. Probing depth (PD) ,attachment loss (AL) ,plaque index (PLI) ,bleeding index (BI) and hypersensitive C reactive protein (hsC‐RP) levels were compared before and after 6 weeks treatment. Results PD ,AL ,PLI ,BI ,and hsC‐RP levels had no significant difference between two groups before treatment (P>0.05). PD ,AL ,PLI ,BI and hsC‐RP levels after treatment in treatment group were all significantly decreased [PD :(2.11 ± 0.28) vs (2.51 ± 0.68) and (2.42 ± 0.31) mm ;AL :(3.18 ± 0.41) vs (3.58 ± 0.83) and (3.49 ± 0.58) mm ;PLI:(0.41 ± 0.21) vs (0.96 ± 0.33) and (0.78 ± 0.26);BI:(0.97 ± 0.32) vs (2.65 ± 1.21) and (1.14 ± 0.30);hsC‐RP :(1.02 ± 0.63) vs (3.29 ± 2.88) and (2.72 ± 1.51) mg/L] as compared with those before treatment in treatment group and those after treatment in control group (P<0.05). Conclusion Levels of inflammatory factors will decrease when periodontic treatment is added to the antidiabetic therapy in T 2DM patients with chronic periodontitis.

Immunohistochemistry examination showed that SOX-2 was expressed in all 23 cases of odontogenic cyst and in 2 cases of poly-cystic ameloblastoma.

Intracellular [Ca+ + ]i was examined using Quin-2. Resting [Ca+ + ]i in platelets loaded with Quin-2 in media containing either 1 mmol/L CaCl2. or 2 mmol/L EGTA was 52.1?5.2nmol/L, 28.4+5.1 nmol/L, respectively. The concentration of external calcium appears to play an important role in cytosolic Cat++ hemeostasis in platelets. Stimulation with A2 3187( 25nmol/L ) caused [Ca++]i to peak at 145?30.8 nmol/L in the presense of extracellular 1mmol/L CaCl2 and 34.4?2.4 nmol/L after addition of 2 mmol/L EGTA. These data suggested that the most of this rise results from calcium entry across the plasma membrane, with a small contribution to the discharge of intracellular pool of Ca++.Anisodamine ( 100?mol/L ) had no effects on resting [Ca + + ]i and A23187-evoked [Ca++]i level in the absence of CaCl2, Anisodamine ( 100?mol/L, 25?mol/L), verapamil ( 10?mol/L ) decreased the A23187 -evoked [Ca++ ]i rises in the presence of CaCl2 by 38%, 34%, 25%, respectively. These results provided a mechanism for the calcium antagonistic effects of Anisodamine.

Objective To compare the durability and economic value of o-phthalaldehyde(OPA)and glutaraldehyde as endoscope disinfectors in continuous use of cleaning machine.Methods Compare the maximal number of endoseopes which could be disinfected by the two disinfectors at its minimal effective concentration,as well as the corresponding costs.The cost-effectiveness of two disinfectors were evaluated with Revenue-Incremental Cost Analysis.Results The number of endoscopes disinfected by OPA were 1.56 times as compared with glutaraldehyde,and an additional 2.95 yuan could be gained with OPA at the cost of 1 yuan.Conclusion When cleaning machine is full loaded,OPA is more durable than glutaraldehyde,and Can gain more benefit at the same cost.

Objective To investigate the role of large conductance calcium-activated potassium channels (BK) in neuronal Ca2+ overload following traumatic brain injury (TBI). Methods Neuronal cells of C57BL/6 mouse cortex were collected and cultured. Patch-clamp technique was applied to investigate the changes of intracellular free calcium [Ca2+] i and firing frequency of neuronal action potentials in rest condition or evoked by 100 pA electric current lasting 500 ms after perfusion of Iberiotoxin ( 100 nmmol/L), a BK specific blocker. The cells were divided randomly into experimental group ( plus Iberiotoxin) and control group. Extracellular solution of cultured neurons was further perfused with KCl (20 mmol/L) to induce elevation of [Ca2 +]i and influence of Iberiotoxin ( 100 nmol/L) on amplitude of [Ca2 +] i elevation was determined. Results No significant changes of neuronal spontaneous action potential frequency and [Ca2 +]i were observed in rest condition after perfusion of Iberiotoxin (P＞0.05).However, when evoked by electric current, the frequency of action potential was (10.4 ± 3.0) Hz,which was increased to ( 13.8 ± 3.7 ) Hz after perfusion of Iberiotoxin, with statistical difference (P＜0.05 ). The [Ca2 +] i level was ( 14.21 ± 16.98 ) nmol/Lbefore perfusion with Iberiotoxin but was increased to (44.07 ± 34. 4) nmol/L after perfusion of Iberiotoxin (P ＜ 0.05 ). Extracellular high concentration of KCl increased [Ca2 +] i of neurons, while perfudion of Iberiotoxin further elevated [Ca2 +]i (P ＜ 0.05).Conclusion BK may play an important role in the regulation of neuronal [Ca2+] i and in neuronal Ca2+overload following TBI.

Objective To explore the applicability of the Perceived Stress Scale(PSS) in Chinese medical students.Methods 773 medical students completed the PSS and the Depression Anxiety Stress Scale (DASS).The reliability and validity of the PSS were analyzed.Results The exploratory factor analysis showed that two factors were extracted from the scale,which could explain 53.74％ of the total variance.The confirmatory factor analysis verified that the structure of the PSS was two-dimensional (x2/df =2.863,CFI =0.943,TLI =0.924,GFI =0.951,AGFI =0.921,RMSEA =0.07).The internal consistency coefficient of the PSS,the perceived stress subscale and the perceived coping ability subscale were 0.858,0.852 and 0.750.The relationship between the PSS and the DASS was significant,which demonstrated that the PSS had a good criterion-related validity.In addition,t-test showed that girls had a higher level of the perceived stress than boys (t =-2.574,P =0.01),and students who lived in rural had a higher level of the perceived stress than students who lived in urban (t =2.788,P =0.005).Conclusion The study indicates that the PSS has a high level of the reliability and validity,and it is worthy of exploring the perceived stress in medical students.

Purpose:To further investigate the role of the novel negative immuno-regulatory molecules in tumor evasion by testing B7-H4 e xpression on tumor tissues and spleen macrophages induced by tumor antigen of mi ce and BTLA expression on T cells of the tumor bearing mice. Methods:Via RT-PCR, we evaluated the differences in the expres sion of B7-H4 mRNA of the tumor cell lines (3LL and Renca) in vitro,tumor t issues and the expression of BTLA mRNA of the tumor bearing mice T cells;via flo w cytometry, we evaluated the differences in the expression of B7-H4 surface pr otein of the spleen macrophages from tumor bearing mice and the normal spleen ma crophages after stimulation with the tumor antigen. Moreover, via immunohistoche mistry , we also evaluated the differences in the expression of B7-H4 surface p rotein of tumor tissues. Results:Tumor cell lines (3LL and Renca) did not express the B 7-H4 mRNA in vitro.All tumor tissues or spleen macrophages in tumor bearing mice have a high expression of both B7-H4 mRNA and B7-H4 protein. Compared wi th those from normal mice, T cells from tumor bearing mice showed a higher expre ssion of BTLA. Stimulated by tumor antigen in vitro, normal mice spleen macr ophages B7-H4 expression increased significantly; while no apparent difference was found in the groups stimulated by the corresponding normal tissue antigen. Conclusions:The aberrant expression of the costimulatory molecu les, B7-H4 and its receptor BTLA, may have a regulative effect in tumor evasion immunity.

0.05).The frequency of action potential evoked by electric current was higher after perfusion of iberiotoxin,as well as thei level during depolarization(n=9,P

OBJECTIVE:To investigate the effects of clinical pharmacists participating in the implementation of clinical path-way under the condition of disease diagnosis related groups-prospective payment system(DRGs-PPS),and to provide reference for promoting rational drug use in the clinic. METHODS:Patients with femoral neck fracture in the clinical pathway were collected from our hospital as research objects. The patient collected during Jan.-Dec. 2015 were included in control group(52 patients includ-ed,41 patients completed)and those collected during Jan.-Dec. 2016 were included in observation group(58 patients included,46 patients completed). Clinical pharmacists participated in the implementation of clinical pathway in observation group,and provided technological intervention and administrative intervention. No intervention was performed in control group. Hospitalization time, hospitalization cost,drug cost and ADR were observed in 2 groups. The rationality of antibiotics for prophylactic use,analgesic drugs,adjuvant drugs,anti-osteoporosis drugs and anticoagulant were compared between 2 groups. RESULTS:After clinical pharma-cists participating in the implementation of clinical pathway in observation group,there was no statistical significance in hospitalization time or the incidence of ADR between 2 groups(P>0.05); hospitalization cost and drug cost of observation group were significantly lower than those of control group,with statistical significance(P<0.05). Medication time and cost of antibiotics for prophylactic use,cost of analgesic drugs,medication time of adjuvant drugs in observation group were significantly shorter/lower than control group;type of anti-osteoporosis drugs was significantly more than control group,with statistical significance(P<0.05). CONCLUSIONS:Under DRGs-PPS,the participation of linical pharmacists participating in the implementation of femoral neck fracture clinical pathway can play an important role in regulating the clinical rational use of 5 kinds of drugs and ensuring the safety,effectiveness and econom-ics of drug use.

0bjective To observe the different brain activation of acupuncture and electroacupuncture on healthy subjects and healthy subjects.Methods The different brain activation involved in heroin addiction between healthy subjects and addicts was detected by fMRI.Acupuncture point used in present study Was Zusanli (ST 36).Results Different brain activations between healthy subjects and addicts during electroacupuncture were hypothalamus(X0,Y2,Z9,t=7.36,P<0.01),anterior cingulate(X5,Y49,Z8,t=4.11,P<0.01),tempo-ral gyrus(X61,Y12,Z8,t=3.05,P<0.01).The difference of activated regions during conventional acupuncture between healthy subjects and heroin addicts was thalamus(X2,Y16,Z12,t=2.87,P<0.01),parahippocampus (X17,Y52,Z3,t=3.14,P<0.01),and hypothalamus(X0,Y2,Z9,t=6.98,P<0.01).Conclusion Regions with significant activation detected by fMRI are different during acupuncture in heroin addicts and in the healthy subjects.Notably,the hypothalamus activation is more robust in the addicts than in the healthy subjects during ac-upuncture stimulation.