AIM: To evaluate the effect of replacing fetal cattle serum with umbilical cord plasma and application of OK-432 on dendritic cells from umbilical cord blood in vitro so as to offer a new technical way of preparation high-powered dendritic-cell-based vaccines for the cancer immunotherapy.METHODS: Human cord blood mononuclear cells(CBMCs) were cultured in RPMI-1640 containing 10% autologous plasma,GM-CSF and IL-4,some of which were supplemented with tumor lysates and/ or OK-432.MTT assay was applied to measure the antigen presenting ability of DCs in allo-MLR.Killing rates of autologous T lymphocytes induced by DCs on different target cells were measured by LDH method.RESULTS: Cells appeared typical morphology of DCs after culture and the allo-stimulate capacity of DCs and the CTL response in vitro were enhanced by treating with tumor lysates and OK-432.CONCLUSION: Mature DCs can be induced from human CBMCs by this means with fewer cytokines and less time.The tumor lysate antigens are captured by DCs treated with tumor lysates and OK-432,and presented to lymphocytes successfully,indicating a new way to develop dendritic-cell-based vaccines for clinical immunotherapy of gastric cancer and other tumors.

Objective To study the safety of hypothermia in neonates with hypoxic-ischemic brain damage after asphyxia. Methods Twenty four full term newborns with Apgar score 0.05). Conclusions The results suggest that the selective head cooling in full term newborn has no significant adverse effect on cardiac function, kidney function and coagulation function.

Objective To explore the effects of glargine combined metformin and repaglinide combined metformin treatment on metabolism of free radicals in elderly patients with type 2 diabetes(T2DM ) .Methods Selected 90 cases of elderly T2DM pa‐tients were divided into 2 groups:group A (glargine combined metformin treatment group) ,group B (repaglinide combined met‐formin treatment group) .Each group had 45 patients ,they were all treated for four weeks in antidiabetic therapy ,select 40 healthy people in physical examination center of hospital as controls .They were measured in patients with fasting plasma glucose (FPG) ,2h postprandial blood glucose(2hPG) ,malondialdehyde (MDA) ,glutathione peroxidase (GSH‐PX) ,8‐iso‐prostane (8‐iso‐PGF2a) , Hcy and so on ,before and after treatment .Results (1)Before treatment ,the FPG ,2hPG ,HbA1c ,MDA ,8‐iso‐PGF2a ,Hcy of group A and B were higher than the control group ,while the level of GSH‐PX was lower than the control group ,the difference was statis‐tically significant(P<0 .05);(2)After treatment ,the levels of FPG ,2hPG ,MDA ,8‐iso‐PGF2a ,Hcy were significantly reduced and the levels of GSH‐PX was significantly elevated in group A ,the differences were statistically significant (P<0 .05);(3)There were no obvious difference in reduce the levels of FPG ,2hPG and HbA1c between group A than group B(P>0 .05) ,While 8‐iso‐PGF2a , MDA ,Hcy of group A had a bigger decline rate than group B ,the GSH‐PX in group A increased more compared with group B ,the difference was statistically significant (P<0 .05) .Conclusion (1)There is a high oxidative stress state in elderly patients with T2DM ;(2)Both treatments could improve diabetics oxidative stress levels ,but glarginecombined metformin to reduce diabetics oxi‐dative stress is superior to repaglinide combined metformin .

BACKGROUND:Airborne pollen is the most important factor to induce the seasonal allergic diseases.The production and dispersal of pollen are closely correlated with the meteorological factors.OBJECTIVE:To observe the correlation between the seven meteorological factors (air pressure,air temperature,humidity,precipitation,wind speed,evaporation capacity and sunshine) and the airborne pollen in Nanchang so as to provide theoretic evidences for preventing and treating anaphylactic disease in that region.DESIGN:Observational experiment. SETTING:Department of Allergic Reaction,Jiangxi Medical College.MATERIALS:An investigation on airbome pollen was performed for a year by Durham gravity method.Data of airborne pollen and the seven meteorological factors in urban area of Nanchang city were collected.METHODS:The top of the 4th experimental building in the southern yard of Jiangxi Medical College located in the center of Nanchang city was taken as exposed point.The pollen was surveyed by Durham gravity method from April 1st,2000 to March 31 st,2001.Meteorological data of the seven factors were collected every day and supplied by the Weather Bureau of Jiangxi Province.The correlation between meteorological factors and airborne pollen was analyzed by the multivariate Jinear stepwise regression analysis with SAS 6.12.MAIN OUTCOME MEASURES:The type,amount,seasonal distribution of airborne pollen and the correlation between the seven meteorological factors and the airborne pollen in urban area of Nanchang city. RESULTS:There were airborne pollens in Nanchang city all the year round,which included about 47 types.The main pollen season of Cupressaceae was in March,Pinaceae in March and April, and Cunninghamia R.Br in March,Chenopodium-Amaranthus and Ambrosia from August to November, Artemisia from September to November and Humulus in September.Gramineae had been scattering all seasons but mostly from June to October.In the correlation analysis between dispersal of airbome pollen and seven meteorological factors, multivariate linear stepwise regression analysis was conducted in some major polien counting in steady dispersal period and seven meteorological factors. The positive correlation was found in Pinaceae with air pressure,air temperature,wind speed and sunlight,and the negative correlation was found with precipitation and evaporation capacity.The positive correlation was found in Cupressaceae with air pressure,air temperature and sunlight,and negative correlation was found with humidity,precipitation,wind speed and evaporation capacity.The positive correlation was found in Ambrosia with air pressure,air temperature and wind speed,while negative correlation was found with humidity,precipitation,evaporation capacity and sunshine.The positive correlation was found in Artemisia with air pressure,air temperature and wind speed,while negative correlation was found with humidity,precipitation, evaporation capacity and sunshine.The positive correlation was found in Gramineae with air pressure and air temperature,while negative correlation was found with humidity,precipitation and evaporation capacity.However,if using count of total pollen in a year or half a year to analyze the relationship,the correlated factors were much fewer than that mentioned above.CONCLUSION:The dispersal of airborne pollen is related with the seven meteorological factors.It is better to use the grain amount of single pollen in its main pollen season to analyze the relationship with meteorological factors.

membrane transporters are a cIass of functionaI membrane proteins and mediate the absorption,distribution and eIimination of many drugs. They are biomoIecuIes responsibIe for the homeo-stasis,and they,however,are easiIy reguIated by many kinds of chemicaIs. The IocaIizations,func-tions,substrates and seIective inhibitors of 18 more understanding transporters among the 26 known ones in the kidney are summarized in this review. The impact of these transporters on drug disposition and the typicaI drug-drug interactions concerned are aIso discussed.

Objective To explore the related factors of hepatitis B virus(HBV) drug resistance and their clinical significance . Methods The retrospective analysis was performed on the dinical data in 153 cases of CHB in our hospital during 2011-2013 .The 8 viral drug resistance loci of 4 known nucleosides analogues lamivudine ,adefovir ester ,entecavir and telbivudine were performed the direct sequencing by using the double DNA end termination method(Sanger method);the related clinical factors of drug‐resist‐ant virus genotypes were statistically analyzed and the correlation between prognosis and drug resistance was analyzed .Results A‐mong 153 patients with CHB ,47 cases(29 .8% ) appeared virus genotype drug‐resistant ,the common sites were 204M‐I(18 cases) , 204M‐V(8 cases) ,followed by 181A‐T(10 cases) ,181A‐V(5 cases) ,then 180L‐M (14 cases) .The proportion of viral drug resist‐ance in the patients with family history ,HBeAg positive ,using the nucleotide analogues in the past and the baseline ALT≥5 times was significantly increased ,the incidence rates were 38 .8% ,34 .8% ,34 .6% and 50 .0% respectively .The multivariable COX re‐gression found that HBV‐DNA genetic drug resistance increased the risk of progression to cirrhosis of the liver ,the OR value was 4 .704(95% CI:1 .199 -18 .454) .Conclusion the Sanger method for direct sequencing is reliable and accurate method of HBV‐DNA genotype drug resistance .The proportion of viral drug resistance in the patients with a family history of hepatitis B ,HBeAg positive ,using nucleotide analogues and baseline ALT≥5 times is significantly increased;HBV‐DNA genotype drug resistance also increases the risk of progression to cirrhosis of the liver .

Objective To investigate the levels of sICAM 1 and sE selectin in patients with chronic hepatitis(CHC) and to study their roles in judge of response to IFN ? 2b treatment. Methods sICAM 1 and sE selectin levels were measured in 32 cases of CHC before and after treatment of IFN ? 2b by enzyme linked immunosorbent assay(ELISA), levels of HCV RNA was detected by quantitative PCR and serum ALT activity was also detected. Results Levels of sICAM 1 and sE selectin in CHC patients were significantly higher than those in normal controls(P

Objective To establish hyperoxia retinopathy in premature rat model with SpragueDawley (SD),observe the pathological changes of angiogenesis and expression of vascular endothelial growth factor (VEGF),and compare the effect of bevacizumab periocular injection and intravitreal injection of retinopathy of premature rats.Methods Ninety preterm rat retinopathy models were randomly divided into 3 groups:high oxygen group,retrobalbar injection group,and vitreous cavity group (n =30 per group);36 healthy rats were chosen as the normal control group.Each group were randomly selected 10 rats 'right eyes to make retinal flat mount to observe the right eye retina vascular morphology;10 resections of right eyes were embedded in paraffin,and stained with hematoxylin eosin (HE);the number of right eye of retinal neovascularization was observed;the right eye RNA was extracted,and reverse-transcription polymerase chain reaction (RT-PCR) was used to detect the expression of VEGF.Results The normal group showed retinal vessels were around a reflection of distribution,good branch,shape rules,and clear structure.Intravitreous neovascularization occurred in the high oxygen group and peribulbar injection group.Retinal vascular endothelial cells in the high oxygen group were increased significantly compared to the normal group (P ＜ 0.05).The visible clusters of red blood cells in the periocular injection group and vitreous group were decreased compared to the high oxygen group (P ＜ 0.05),but no significant difference between the periocular injection group and vitreous group (P ＞ 0.05).The expressions of VEGF mRNA and HE staining had a consistent trend.Conclusions Bevacizumab inhibited the expression of VEGF that was increased in premature rats in hyperoxia retinopathy.The effects of bevacizumab periocular injection and intravitreal injection were similar,but the former was more secure than the latter.

Objective To evaluate the value of amplitude-integrated EEG(aEEG)as a quantitative predictor of neurodevelopment outcome of full-term infants with hypoxic-ischemic encephalopathy(HIE).Method EMBASE,Ovid,PubMed,Cochrane Library,Springer Database,CNKI,CSJD and Wanfang Database were searched from when the database was founded to June 30,2010 for studies about aEEG as a predictor of neurodevelopment outcome in full-term infants with HIE.Characteristic information of each articles was extracted and QUADAS items were used to evaluate the qualities of included studies.Meta-disc software 1.4 was used to perform the metaanalysis.All included studies were combined with weighted quantity; sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and their 95 ％ CI were calculated.Results Eleven studies were eligible for the primary meta-analysis and totally 717 subjects were included.There was an overall sensitivity of 86％(95％ CI:81％-89％),specificity of 90％(95％ CI:86％-93％),positive likelihood ratio of 7.70(95％ CI:5.69-10.43),negative likelihood ratio of 0.17(95％ CI:0.13-0.22)and the area under the curve(AUC)of summary receiver operating characteristic curve was 0.95 for aEEG tracings to accurately predict poor outcome of HIE in full term neonates.Conclusion aEEG is a valuable bedside tool for predicting long-term neurodevelopment outcome in term infants with HIE.

Objective: To observe the eff ect and mechanism of chronic high-fat diet on predation behavior in rats. Methods: Ten female SD rats with 4-week-old were randomly divided into a normal control group (NC group,n=5) and a chronic high-fat diet group (HF group,n=5). The rats in the NC group received the regular diet while rats in the HF group were fed with high-fat diet. Fitf een weeks later, the predation behavior of rats was evaluated by open if eld test and food foraging tests. At the end of experiments, the rats were killed and brain tissues were collected for evaluation of c-Fos protein expression in anterior cingulate cortex by immunohistochemical assay. Results: hT e predation behavior of rats in the HF group was signiif cantly impaired in the competitive or non-competitive food foraging test compared with the control rats (P<0.001). hT e c-fos protein expression in anterior cingulate cortex of rats from the HF group was signiif cantly decreased (P<0.001). Conclusion: Long time high-fat diet can aff ect the predation behavior of rats, which is related todysfunction of neuron in anterior cingulate cortex.