Objective To compare and evaluate the defect-repaired capabilities of human bone morphogenetic protein-2(hBMP-2) gene modified tissue engineered bone in the segmental bone defect model of rabbit's radius.Methods Rabbit's bone mesenchymal stem cells (BMSCs)were transferred with hBMP-2 gene through Adeno-XTM adenoviral expression systems,then seeded onto the compound scaffold of calcium phosphate cemept(CPC)and fibrin glue(FG)to construct a new kind of gene modified tissue engineered bone after proliferation in vitro for three weeks(Group A).Meanwhile,the compound scaffold of calcium phosphate cement(CPC)and fibrin glue(FG),which were seeded by rabbit's bone knesenchyrmal stem cells(BMSCs) after proliferation in vitro for three weeks(group B)and the compound scaffold without cells(Group C)acted as control groups.Then,three kinds of reconstructive modalities were implanted into segmental bone defect of donator rabbit's radius.Besides these three groups,bone defect model of rabbit's radius without treatment(Group D)represented blank group.The defect-repaired capabilities were assessed by gross observation,radiograph,Single Photo Emission Computed Topography (SPECT)and histological analysis in the 4th week,8th week and 12th week after operation.The rates of bone healing in the different groups were compared each other.Results All defects that had been treated with implants(Group A,B,C)exhibited new bone formation and could attain osseous tissue healing 12 weeks after operation,but defects in blank group(Group D)were repaired only by fibrous tissue.The defects in the Group A regenerated more new bone,bridged earlier and stronger than those in the Group B and Group C.The quantity and rate of new bone formation in the Group B and Group C had no significant difference and the rates of bone healing in different groups showed the same results.Conclusion hBMP-2 gene modified tissue engineerod bone have better potential to form new bone and the rate of bone healing in repairing bone defects is higher,so this way is an optimal kind of material for artificial bone graft.

Objective:To investigate the effect of silver diamine fluoride(SDF)on the bonding strength between dentine and glass ion-omer cement(GIC).Methods:1 2 extracted sound molars were prepared into dintine samples and distributed into sound dentine group and demineralized dentine group.According to the treatment methods,the samples in each group were respectively divided into 3 sub-groups:A(control group),B[coated with 38% Ag(NH3 )F2 ]and C(SDF treatment with additional lighting-curing)(n =20).Then a hand-mixed conventional glass ionomer cement Fuji IX was placed on the dentine surface.After 24 h,micro tensile bond strength test and scanning electron microscope (SEM)analysis were conducted.Results:The bonding strength of demineralized dentine was higher than that of sound dentine(P <0.01 ).SDF with additional lighting-curing treated dentine showed a higer bonding strength value than only SDF treated dentin(P <0.01 ).Conclusion:SDF may improve the bonding between dentine and GIC.

Gastrointestinal stromal tumors (GISTs) are relatively common mesenchymal tumors of the digestive tract characterized by c-kit mutations and the stomach is the commonly involved site.Clinical and pathological diagnosis of GISTs can be achieved by B sonography,computed tomography and immunohistochemical detection of marker CD117.Surgical resection of GISTs has been the most effective therapy.Target therapy with tyrosine kinase inhibitors may reduce the development of recurrence or progression of GISTs.The optimized method of diagnosis and treatment of huge GISTs is still controversial.In this paper,the diagnosis and treatment for huge GIST combined with large right inguinal hernia were discussed.

ObjectiveTo investigate the correlation between HBsAg, prothrombin time activity (PTA), and indocyanine green retention rate at 15 minutes (ICG R15) in patients with HBeAg-positive chronic HBV infection. MethodsA total of 92 patients with HBeAg-positive chronic HBV infection who were admitted to The First Hospital of Lanzhou University from December 2015 to April 2016 were enrolled and divided into chronic hepatitis B (CHB) group (24 patients), compensated liver cirrhosis group (38 patients), and decompensated liver cirrhosis group (30 patients). Serum HBsAg quantitation, PTA test, and liver reserve function test (ICG R15) were performed for all patients. The chi-square test was used for comparison of categorical data between groups, an analysis of variance was used for comparison of continuous data between multiple groups, and a Pearson correlation analysis was also performed. ResultsThere were significant differences between the three groups in serum HBsAg quantitation (3.82±0.43 log10IU/ml vs 2.88±0.36 log10IU/ml vs 2.60±0.27 log10IU/ml, F=25.19, P＜0.001), ICG R15 (7.51%±3.10% vs 9.57%±8.18% vs 24.13%±14.28%, F=24.00, P=0.001), and PTA (8100%±1762% vs 83.08%±9.64% vs 62.32%±16.90%, F=13.42, P=0.009). The correlation analysis showed that PTA was negatively correlated with ICG R15 in all three groups (r=-0.948, -0.602, and -0.735, all P＜0.01). In the compensated liver cirrhosis group and decompensated liver cirrhosis group, HBsAg was positively correlated with PTA (r=0.410 and 0.473, both P＜0.05) and negatively correlated with ICG R15 (r=-0.427 and -0.768, P＜0.01). ConclusionIn HBeAg positive patients, there are certain correlations between HBsAg, PTA, and ICG R15, which, to a certain degree, reflects the liver reserve function in patients with chronic HBV infection.

Toll-like receptor 4 (TLR4) is an important pattern recognition receptor and plays an important role in the inhibition of hepatitis B virus (HBV) infection. It identifies the HBV-related proteins and produces inflammatory factors, such as tumor necrosis factor-α, interleukin-6, and interleukin-12, and type I interferon through the myeloid differentiation factor 88 (MyD88) and the non-MyD88 pathway, and thus TLR4 exerts its antiviral effect. Meanwhile, it has been found that TLR4 regulates the balance between T helper 1 and T helper 2 cells, promotes the adhesion of CD8+ T lymphocytes in the liver, induces the secretion of inducible nitric oxide synthase, and thus inhibits HBV. If overexpressed, TLR4 will aggravate liver inflammation by excessively activating the body's immune system, aggravating liver ischemia/reperfusion, and activating hepatic stellate cells. This article reviews related articles published at home and abroad in recent years, discusses the mechanisms of action of TLR4 in the pathogenesis of hepatitis B, and summarizes the association between TLR4 and hepatitis B, in order to find new therapies for hepatitis B and new protocols which delay the progression of hepatitis B to liver cirrhosis and liver cancer through the studies on the association between TLR4 and hepatitis B.

Objective? To explore the distribution of Myers and Briggs Types Indictor(MBTI) personality type and status quo of professional identity in senior nursing undergraduates. Methods? Totally 230 full-time nursing undergraduates from 4 medical universities in Beijing were selected by convenient sampling between March and July 2017 and investigated with the general information questionnaire, MBTI-M Personality Type Scale and Professional Identity Scale for Nursing Undergraduates. Totally 230 questionnaires were distributed and 201 valid questionnaires were collected. Results? The professional identity score of the 201 senior nursing undergraduates was (83.40±10.47). Multivariate linear regression analysis revealed that registered county household, first choice, choice of nursing for personal love, positions of class or university and ESFJ personality had effects on professional identity of senior nursing undergraduates (P＜ 0.05). Conclusions? The professional identity of senior nursing undergraduates remains at an average level. The professional identity of nursing students with ESFJ personality is higher than that of nursing students with other personality types. Nursing educators should pay attention to the difference of professional identity caused by nursing students' personality type, improve the professional identity of nursing undergraduates, and stabilize the nursing team with targeted measures.

Objective:To analyze the research status of the midwifery in China from 2012 to 2021, and to explore the research hotspots and research trends in the midwifery in China in the past 10 years.Methods:The CiteSpace software was used to analyze the time distribution, authors, institutions and keywords of related studies in the field of midwifery published in nursing core journals in China. The search period was from January 2012 to December 2021.Results:A total of 442 articles were included, with the largest number of papers published in 2014 and 2015, and no increase in the number of papers published after 2016. The core author group in the field of midwifery was basically formed, with close cooperation among authors and high output from the five cooperative groups, but little cooperation among teams. Cooperative research with regional institutions began to take shape, and inter-regional collaborative research was relatively scarce. The hotspot keywords in the field of midwifery were "midwife", "pregnant woman", "natural childbirth", "obstetric nursing" and "delivery outcome".Conclusions:The development of research in the field of midwifery in China is slow, and cooperation between teams, institutions and regions needs to be further strengthened in the future. Strengthening the capacity building of midwifery professionals is the key direction of future research, and obstetric nursing is the main trend of future research.

Objective:To explore the research hotspots and frontiers of heart failure nursing in the past 10 years in the Web of Science database.Methods:The studies on heart failure nursing included in the Web of Science database from January 1, 2011 to December 31, 2020 were eligible for the study. The CiteSpace visualization software was used to analyze the included articles to determine the development context and research hotspots of heart failure nursing.Results:A total of 1 949 articles were included. In the past 10 years, the number of articles published in the field of heart failure nursing maintained an upward trend year by year. The United States had the most publications, highest importance and centrality of heart failure nursing. In the past 10 years, the research hotspots of heart failure nursing focused on the quality of life, self-care and palliative care in patients with heart failure. Research frontiers focused on readmissions of heart failure patients, symptom clusters, and caregivers.Conclusions:Heart failure nursing developed rapidly. Research efforts should be further intensified, the areas of cooperation should be broadened, and research hotspots and frontiers of heart failure nursing should be focused, so as to promote the development of heart failure nursing research in China.

The gentamicin sulfate carried calcium sulfate (GSCS) implants were fabricated by the coagulating method, and the release rate of the gentamicin tested by UV-spectrometer and the absorbing rate of the calcium sulfate carrier in vitro were studied. The release patterns of two types of GSCS were compared. The trend of daily weight loss of GSCS was found being similar to that of pure calcium sulfate, which suggested that the gentamicin part has little effect on the absorbing pattern of calcium sulfate. The release rate of gentamicin is controlled by the erosion rate of calcium sulfate, so GSCS with different amount of gentamicin has the same release patterns. The DRP value of ED is higher than that of CO during the early stage, while the DRP value of ED is lower than that of CO during the late stage. The GSCS samples were implanted into the defect mold on the radii of the rabbits to investigate the potential for the use of GSCS implants as bone fillers, and the results revealed that new bone had been induced in a great part of the defect at 14 weeks after operation.
Animals ; Anti-Bacterial Agents ; administration & dosage ; pharmacokinetics ; Bone Regeneration ; drug effects ; Calcium Sulfate ; administration & dosage ; Gentamicins ; administration & dosage ; pharmacokinetics ; Implants, Experimental ; Rabbits ; Radius Fractures ; therapy

Compared with people with non-sensitive skin,people with sensitive skin are more likely to have some undesirable feelings and symptoms in response to stimuli from the external environment,such as itching and pain sensations,and even erythema after chemical stimulation of the skin.Few reviews are related to evaluation methods for sensitive skin in China and other countries.This review introduces subjective,semi-subjective and objective evaluation methods for sensitive skin,summarizes evaluation methods suitable for different types of sensitive skin,and analyzes advantages and limitations of various evaluation methods.